A method of treating a vast circular burns iiib-iv in a mammal in experiment

 

(57) Abstract:

The invention relates to experimental medicine and can be used to treat a wide circular burns IIIB-IV in mammals in the experiment. Produce selection burned skin area not less than 30% of the entire surface of the skin by applying two encircling polnoletnyh cuts to the superficial fascia along the edge of the burn. Beyond the inner edge of the wound pull together a single set of skin and subcutaneous tissue in the form of a cylindrical pipe with superficial fascia, twisting out of the inner side surface. Move turned edge in the longitudinal direction up to a complete separation of burned tissue. Then after hemostasis immerse the animal's body, excluding the head, into the container periodically replaced with a sterile salt water solution. The temperature of the solution to 36.8oC. In a salt solution add a combination of chemotherapy drugs. The method allows to extend the life of experimental animals with better quality. 1 C.p. f-crystals.

The invention relates to experimental medicine.

The literature describes that the removal of burned skin on the entire thickness without subsequent cutaneous Century, Nasilowski Century , Sitkiewicz K. - M.: Medicine, 1980, S. 376). When comparing the effects of thermal burn 15-20% of the body surface and remove the appropriate number of healthy skin was found significant similarities adverse changes arising in either case (Kochetygova N. And. Comparative characterization of the effects of thermal destruction and removal of the skin on the vast square in the experiment. The experts. Hir., 1963, 4, S. 6-9).

Given the strong trauma and profuse bleeding, early necrectomy over an area of 10% of the entire surface of the body do not produce (yudenitch centuries the Treatment of burns and their consequences: the Atlas. - M.: Medicine, 1980, S. 192).

Meanwhile, it is known that early amputation burnt limbs, when you remove the source of intoxication and it is not accompanied by the formation of extensive wound surface, leads to a reduction in mortality of animals (Kochetygova N. And. Early necrectomy extensive burns - experimental study. Journal of surgery, 1963, 5, S. 88-92). The essence of this method consists in the surgical removal (amputation) body parts - rear limbs together with burned skin.

The technical result of the claimed invention avleen with better quality at the expense of one-stage surgical removal of burned skin, selected two surrounding Dolnoslaskie surgical incisions to the superficial fascia that runs along the edge of the burn. Skin removal is carried out by the contraction of the single complex of skin and subcutaneous tissue in the form of a cylindrical pipe with the superficial fascia. This force is applied to the inner edge of the wound, twisting him out of the inner side surface of the complex and moving the turned edge in the longitudinal direction up to a complete separation of burned tissue. Then after hemostasis immerse the animal's body, excluding the head, into the container periodically replaced, sterile salt water solution temperature 36,8oWith containing chemotherapeutic agents.

The essence of the proposal is that in the present method under anesthesia, soon after the heat defeat, is simultaneously Dolnoslaskie surgical removal of burned skin and subcutaneous tissue disorders common complex to the superficial fascia. After hemostasis, the animal's body (except the head) immersed in a container filled with salt water solution (solution temperature 36,8oC) ion concentration (mEq/l): Na+- 144; K+- 4; With the left solution is added to the combination of chemotherapy drugs namely metronidazole at a concentration of 16 mg per 1 l of saline and ciprofloxacin at a concentration of 12 mg per 1 l of saline solution. This combination of chemotherapy drugs eliminates the infection and inflammation. Change salt solution with chemotherapeutics are changed every 4 hours. As a result, the source of burn intoxication radically removed, no intoxication, pain impulses are reduced, the animal swims in the solution, which prevents bedsores, as evenly distributed fluid pressure less than the pressure of the body on the surface under normal conditions. Conclusion the wound surface in a salt solution prevents necrosis of the exposed tissue, the maceration of the epidermis and reduces pain. In aqueous isotonic environment significantly reduced the plasma loss. No scab makes movement painless, easy care and observation of the wound surface, thus eliminating the need for bandaging. All together improves the condition of the animal and increases life expectancy.

In the available literature on the biology and medicine of references about the simultaneous destruction of the complex burned skin and podem animal body for the entire period after necrosectomy in a special saline with chemotherapeutics (while maintaining a certain temperature of the solution) is not met, that allows us to consider the claimed invention meets the criterion of "novelty."

Example.

The experiment was conducted on 15 white rats-males line "Wistar" weighing 160-170 g with a pre-amputated tails. All animals under General anesthesia was performed plucking skin on the entire surface of the body, excluding the head. Then to exclude burns of internal organs under the skin and the subcutaneous fat by well-known methods (Kochetygova N. And. Burn disease. (Essays on pathological physiology). - L.: "Medicine" - 1973, 248 C.) using a syringe was injected air. In boiling water successively immersed dorsal, lateral and ventral surface of the body. The time of immersion of each side was 9 seconds. The total area of the burn IIIB-IV degree was not less than 30% of the total body surface. Next, the animals were divided into 3 groups of 5 rats each. The first group of animals after applying burn additional impacts were not exposed, and the rats were placed in sterile cell-heater (as the litter was used previously dried and sterilized fine pine shavings) with a temperature gradient in diversity-remoted from the panel of heater points from 22 to 3i. Remove the burnt outer cover by scalping in "stocking". To do this, on the limits of the burn spend 2 polnoletnyh encircling skin incision, after which the complex tissue in the form of a cylindrical pipe is pulled together with the superficial fascia. This technique allows to reduce blood loss. After hemostasis, the animals are placed in the same sterile cells heaters, as the animals of the first group. Animals of the third group after removal of burnt tissue in the same manner as in the second group, is the immersion of the animal in a container in a vertical position or at an angle not exceeding 45o. The container filled with sterile salt water solution (solution temperature 36,8oC) with the concentration of ions (mEq/l): Na+- 144; K+- 4; Sa2+to 2.5; Mg2+- 1,5; CL-- 114; HCO3-- 30; NRA42-- 2; SO42-- 1. In saline is added to the combination of chemotherapeutic agents, namely metronidazole in the amount of 16 mg per 1 l of saline and ciprofloxacin in the amount of 12 mg per 1 L. of Change of the solution was performed every 4 hours around the clock. The head of the animal was maintained above the surface of the solution.

Animals glucosamin").

Rats of the first two groups were formed dry scab, animals were lethargic, ate a little food, almost did not make motions. In contrast, the rats of the third group immediately after recovery from anesthesia began to move, to eat. After 5-7 days started to actively try to leave the container with a solution. Manipulation of the rats in this group did not arouse bouts of severe aggression.

The life span of rats of the first group was 1-2 days, the second group of 5-7 days, the third group of animals all rats lived for 12 days and were removed from the experiment by an overdose of ether.

1. A method of treating a vast circular burns IIIB-IV in mammals in the experiment, including one-stage surgical removal of burned tissue, characterized in that the produce selection burned skin area not less than 30% of the entire surface of the skin by applying two encircling polnoletnyh cuts to the superficial fascia along the edge of the burn, then for the inner edge of the wound pull together a single set of skin and subcutaneous tissue in the form of a cylindrical pipe with superficial fascia, twisting out of the inner lateral surface, and move turned the animal, excluding the head, into the container periodically replaced, sterile salt water solution temperature 36,8With containing chemotherapeutic agents.

2. The method according to p. 1, characterized in that the salt water solution use the following ion concentration (mEq/l): Na+144; K+4; Sa2+2,5; Mg2+1,5; CL-114; HCO-330; NRA2-42; SO2-41, a salt solution is added a combination of chemotherapeutic agents, namely metronidazole at a concentration of 16 mg and ciprofloxacin at a concentration of 12 mg per 1 l of solution, respectively.

 

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