Medical-preventive cosmetic

 

The invention can be used in the field of cosmetology. The product contains water, auxiliary components, preservative and antimicrobial additive consisting of 0.1 to 1.0 wt.% lysozyme egg white and 0.05-0.5 wt.% ethylenediaminetetraacetic acid or its salts. Cosmetic made on the proposed ratio of ingredients, can effectively deal with the skin microflora responsible for the occurrence of acne and pimples, and oily scalp. 8 table.

The present invention relates to the field of cosmetology and can be used for the prevention and treatment of skin diseases caused by microorganisms.

Known use in medical cosmetics as a natural antimicrobial additives - farnesol [1].

The disadvantages of the use of farnesol can be attributed to the narrow range of actions conditionally pathogenic and pathogenic microorganisms, in particular, according to the company "Dragoco" farnesol absolutely ineffective against pathogenic microorganism E. coli (Escherichia coli) is the causative agent of enteric infections and pathogen Candida albicans will excite the whether in medical cosmetics antimicrobial additives - ketoconazole [2], for example, in the form of "Nizoral-shampoo".

The disadvantages of the use of ketoconazole include a wide range of side effects on the human body.

Contraindications: hypersensitivity, severe liver disease, pregnancy, lactation.

Restrictions for use: the reduced function of the adrenal cortex and pituitary gland, severe renal and hepatic failure.

Side effects: nausea, vomiting, diarrhea, toxic hepatitis, transient increase in transaminases in the blood, headache, dizziness, drowsiness, photophobia, gynecomastia, decreased libido, oligospermia, hair loss, allergic reactions (urticaria, itching, arthralgia, paraesthesia, hyperthermia, and others).

The aim of the invention is the extension of the spectrum harmless natural antimicrobial additives and effectiveness of preventive and curative cosmetics.

To achieve this goal it is proposed to enter into their composition of 0.1 to 1.0 wt. % of egg white lysozyme, as well as to stabilize the activity of lysozyme to use 0.05 to 0.5 wt.% ethylenediaminetetraacetic acid or its salts.

A certain ratio of these ingredients positronic cells (including conditionally pathogenic microorganism E. coli (E. coli) cause intestinal infections and pathogen Candida albicans is the causative agent of candidiasis of the mucous membranes), which is a natural protective component of the human body and animals, closely related with the normal functioning of the immune complex organism [3-6] , as well as to increase the effectiveness of preventive and curative cosmetics.

Implementation of the proposal is demonstrated by the following examples: Example 1.

1.1. To determine the effectiveness of lysozyme in cosmetic products (test method).

Assuming that the level of activity of lysozyme, a measure of the initial rate of the enzymatic hydrolysis of the substrate) in medical cosmetics is not necessary, but more important parameter is the efficiency of its actions (measure the depth of the enzymatic hydrolysis of the substrate), it is proposed test method for the determination of its effectiveness in finished form.

The method is based on the ability of lysozyme to lyse (dissolve) the biomass of the test object. The effectiveness of lysozyme determined by the level of decrease in optical density of the suspension of the microorganism Micrococcus lysodeikticus (test object).

1.2. Testing.

1.2.1. The preparation of an aqueous extract of preventive and curative cosmetics.

The hanging cosmetic products (gel, cream) or an exact volume (liquid) containing 0.1 to 1.0 wt.% lysozyme, placed in a glass chemical glass, make this amount of distilled water to the final concentration of lysozyme in solution was 0.5 mg/ml of the resulting mixture is stirred on a magnetic stirrer for 30 min for extraction of lysozyme.

1.2.2. Preparation of suspension of the test object.

The microorganism Micrococcus lysodeikticus suspended in 0.05 M Tris-buffer pH 7.0. The optical density of the suspension is measured on fotoelektrokalorimetry type ck-2 or spectrophotometer at a wavelength of 590-600 nm in a cuvette with the distance between the walls 1 see the Optical density of the suspension of the test object must be in the range of 0.7-0.8.

1.2.3. Testing.

The reaction is carried out in ultramontane at a temperature of 37oC. For testing use four glass tube, into which is poured on to 9.0 ml of a suspension of the test object. Tubes thermostatic for 15 minutes In three experimental tubes poured in 1.0 ml of extract cosmetics, and in contraindicate stirring the contents.

The test result is assessed visually. In the experimental tubes suspension of the test object should be dissolved. Slight opalescence of the solution caused by the auxiliary components of the studied cosmetics.

Example 2.

The compositions of preventive and curative cosmetics with egg white lysozyme.

Currently, there are many cosmetic products for various applications. However, information about the principles of creation health care means using bio-tests for quality control and efficiency of their finished forms could not be found. Based on the above, the authors propose the principle of creating an entire field of preventive and curative cosmetics, based on the use as antimicrobial additives of egg white lysozyme using test method for monitoring the quality and effectiveness of the finished cosmetic products. The use of various auxiliary materials (emulsifiers, preservatives, thickeners, geleobrazovanie, substances which improve the hair structure, nutrients and others) can improve the decorative properties of the generated cosmetics / products is responsible for the effectiveness of cosmetic products in General.

Example 3.

The study of stability of lysozyme in the finished forms of preventive and curative cosmetics.

To study the stability of lysozyme in the finished forms of preventive and curative cosmetics used the method of "rapid storage", the principle of which is that the higher the storage temperature of 10oWith the speed of a chemical reaction doubles.

3.1. The stability of lysozyme in the liquid cosmetics (lotions, milk).

To study the stability of lysozyme in the liquid cosmetics used the composition presented in table. 1.

Test liquid cosmetics was carried out at different temperatures, the incubation time of the samples corresponds to 12 months of storage under normal conditions. The stability of lysozyme was evaluated by the test method. The results are given in table. 2.

From the above table.1 data shows that during storage of the samples liquid cosmetics at temperatures up to 40oSince lysozyme is stable in the time period corresponding to 12 months of storage under normal conditions. At higher temperatures, it rapidly loses its activity.

To improve stability Lys ratios. The experiment was carried out at a temperature of 50oWith in a period of time corresponding to 12 months of storage under normal conditions. The results are given in table. 3.

From the data given in table. 3, it is seen that ethanolamines acid at a concentration of from 0.05 wt. % and above is a stabilizing agent for lysozyme in liquid cosmetics.

3.2. The stability of lysozyme in a gel-like cosmetic products (gels, balms, shampoos).

To study the stability of lysozyme gel in cosmetics used the composition presented in table. 4.

Test gel-like cosmetic products was carried out at different temperatures, the incubation time of the samples corresponds to 12 months of storage under normal conditions. The stability of lysozyme was evaluated by the test method. The results are given in table. 5.

From the above table.5 data shows that during storage of the samples gel cosmetics at temperatures up to 40oSince lysozyme is stable in the time period corresponding to 12 months of storage under normal conditions. At higher temperatures, it rapidly loses its activity.

To improve the stability of lysozyme ISP conducted at a temperature of 50oWith in a period of time corresponding to 12 months of storage under normal conditions. The results are given in table. 6.

From the data given in table. 6, it is seen that ethanolamines acid at a concentration of from 0.1 to 0.5 wt.% is a stabilizing agent for lysozyme gel in cosmetics.

Example 4.

The study of the biological effects of preventive and curative cosmetics.

4.1. The study of the action cleansing lotion containing lysozyme egg white on the skin.

In experiment took part 15 volunteers (men and women) from 13 to 45 years. The lotion used twice a day, rubbing my face in the morning and evening during the month. The lotion is given in table. 7.

For reviews of subjects lotion did not cause irritation to the skin, facilitated the removal of acne (especially in adolescents), smoothing the skin, removed the irritation after shaving (men) and gave the skin a healthy appearance.

4.2. Studying the effects of therapeutic balm containing egg white lysozyme, seborrhea of the scalp.

The experiment involved 10 volunteers (men and women) from 13 to 40 years with varying degrees of defeat hairy is amitie hair, massage gently rubbed into the scalp and distributed throughout the length of the hair, stood in for 10-15 minutes and washed with water. Processing conducted in the month. Part of the healing balm is given in table. 8.

Reviews on the subjects of healing balm did not cause discomfort. The scalp after 3-4 treatments was clean (except for people with a strong degree of destruction seborrhea). People with severe defeat seborrhea processed healing balm of the same composition, but containing 1 wt.% lysozyme. By the end of the processing differences between people with varying degrees of defeat seborrhea is almost not observed. Thus, therapeutic effect of applying the balm obvious.

Sources of information 1. Farnesol - natural bacteriostatic selective effect - Beauty for professionals, 2000, 9 (85), S. 39-40.

2. Ketoconazole - Register of medicines. 2000.

3. Jolles P. - the Relationship between chemical structure and biological activity of lysozyme, chicken eggs and litzkow from other sources - in collection: protein Chemistry, M., Mir, 1969, S. 5-22.

4. Podbornov C. M. - Comparative characteristics of litzkow different origin, Antibiotics and chemotherapy, 1990, T. 35, 8, S. 22-25.

7. Malamy, M., et al.: Biochemistry, v.3, 1964, p.1893.

Claims

Medical-preventive cosmetic product, including water, auxiliary components, preservatives and natural antimicrobial additive, characterized in that as an antimicrobial additives it contains egg white lysozyme in an amount of 0.1 to 1.0 wt. % and additionally as a stabilizing additive - ethylendiaminetetraacetic acid or its salts in a quantity of 0.05-0.5 wt. %.

 

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