Device for dividing embryos

 

The invention relates to biotechnology, in particular, to the design of devices for handling of cells and embryos. The device includes a cutting element and micropipette fitted sleeve with a concave hemispherical socket, and a cylindrical hole, and placed in the sleeve with a rectangular frame. Micropipette made in the form of semi-cylinders with pistons and rods, provided with lugs and the joint plane sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve, with the formation of the cylinder inner wall. The upper ends of the flat sides of micropipettes made in the form of concave semicircle. In moving the frame over a hemispherical socket sleeve is cutting element, and under the lower ends of micropipets rods which, by means of detachable locks mounted on the rectangular frame - feed screw. The device is comfortable to use and does not require much time. 1 Il.

The invention relates to biotechnology, in particular to a device for carrying out manipulations on the reconstruction of the embryo.

A device for dividing embryos, including the pipette when the covered ZP and micropipette for the expulsion of the embryos from the shell [the book. "Embryo transfer and genetic engineering in livestock". A. C. kvasnitsky etc. - Kiev, "Harvest", 1980, S. 167 - prototype] . When working with device requires high qualification of the operator, and a great time.

The known device in connection with the need for high skill of the operator and the great complexity of working with him when dividing embryos do not permit this operation is required for agricultural production volumes. You need a device which is simple, convenient and provide a minimal investment of time.

This technical result is achieved in that the device for dividing embryos, including a cutting element and micropipette fitted sleeve with a concave hemispherical socket, and a cylindrical hole, and placed in the sleeve can move it with a rectangular frame. Micropipette made in the form of semi-cylinders with pistons and rods. In addition, micropipette equipped with stops and merged flat sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve, with the formation of the cylinder inner wall. The upper ends of the flat sides of micropipets m Fulci is cutting element, and under the lower ends of micropipets rods which, by means of detachable locks mounted on the rectangular frame - feed screw.

The drawing shows a device for dividing embryos, including two semi-cylindrical micropipette 1 being folded flat sides form a cylinder with an internal partition. The upper ends of micropipets 1 provided with lugs 2 and bonded to each other through their input in the Central cylindrical hole of the fixing sleeve 3 with a concave hemispherical socket 4, located above the ends of the inserted micropipettor 1. The upper ends of the flat sides of micropipets 1 made in the form of concave semicircle. In the sleeve 3 can move fixed, movable frame 5 made in the form of a rectangle, one side of which (over the nest 4) fixed removable cutting element 6 with an adjusting screw 7, is rigidly connected with the base of the cutting element and providing the required distance from the edge of the cutting element to the embryo when setting up the device. On the opposite side of the frame 5 is fixed by a screw connection of the feed screw 6, which interacts with the lower ends of micropipets 1, in which the mouth is a device may be fastened by a screw connection screw 8 in the stand 12, providing free access to the screw head 8. The embryo 13 is located in the cavity of the upper ends of micropipets 1, based on their (micropipettes) dual flat sides. The Assembly of the device is performed as follows: two micropipette 1, United flat sides, are introduced into the fixing hole of the sleeve 3 to the stop 2, while the piston 9 micropipettor 1 are in the top position, and releasable locks 11 of the rod 10 is fixed on the frame 5, the feed screw 8 when it is screwed all the way into the lower ends of micropipets 1. After Assembly, the device can be fixed by fixing sleeve 3 in the tripod and secure on the stand 12 of the screw. 8. In the first case, the design of the development should provide a sufficient coefficient of friction between the bushing and frame, and in the second case, the screw connection 8 with stand 12 should be a screw. The whole device is operable in a manual version, i.e. without pinning. In this case, the operator holds the assembled device for micropipette, lowers the embryo into the slot 4 and powercivil screw 8. Frame 5 slides over the screw 8 down together with the cutting element, cutting the embryo.

Adjustment of the cutting elem: while in the upper cavity of micropipets 1, the embryo 13 being in the shell and based on twin-selected in the form of a semicircular flat side of micropipets 1, ready for operation. The cutting element 6 by means of a screw 7, rigidly connected to the base of the cutting element is lowered and fixed in the immediate vicinity of the shell of the embryo 13. The need to adjust the position of the cutting element may occur after leveling (grinding) or replacement of the cutting element. The adjustment is done under a microscope. Assembled and adjusted, the device is ready to work.

The device operates as follows. The embryo 13 with a drop of medium, placed in a hemispherical socket 4 is rolled into a recess (cavity) over the ends of micropipets 1. Screwing the feed screw 8, placing the movable frame 5 in the lower position, fixed on the frame 5, the rod 10 of the piston 9 is moved together with the frame 5 down, creating a vacuum in the cavities of micropipets 1 under the embryo 13. Fixed embryo 13 dissected cutting element 6, since the movement of the frame 5 down cutting element 6 under its influence also moves down. Half of the embryo 13 are sucked into the micropipette. For removal of micropipets 1 detachable locks 11 are exempt from semiplane with movable frame 5, the Ute and conduct further manipulation, associated with quality assessment, cultivation, transplanting, and so on,

Next is the Assembly of the device with new or vacated used micropipette and is the process of dividing the next embryo.

Thus, this device allows you to simplify the process of division of the embryo so that it does not depend on skill of the operator.

This simplicity and the minimum time of the entire process of dividing allow you to use this device in an industrial environment.

Claims

Device for dividing embryos, including a cutting element and a micropipette, characterized in that it is provided with a sleeve with a concave hemispherical socket and a cylindrical bore and placed in the sleeve can move it with a rectangular frame, and micropipette made in the form of semi-cylinders with pistons and rods and provided with stops, United plane sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve to form a cylinder with an inner wall, the upper ends of the flat sides of micropipettes made in the form of concave semicircle, in moving pramogos, the rods which, by means of detachable locks mounted on the rectangular frame - feed screw.

 

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