Device for dividing embryos
The invention relates to biotechnology, in particular, to the design of devices for handling of cells and embryos. The device includes a cutting element and micropipette fitted sleeve with a concave hemispherical socket, and a cylindrical hole, and placed in the sleeve with a rectangular frame. Micropipette made in the form of semi-cylinders with pistons and rods, provided with lugs and the joint plane sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve, with the formation of the cylinder inner wall. The upper ends of the flat sides of micropipettes made in the form of concave semicircle. In moving the frame over a hemispherical socket sleeve is cutting element, and under the lower ends of micropipets rods which, by means of detachable locks mounted on the rectangular frame - feed screw. The device is comfortable to use and does not require much time. 1 Il. The invention relates to biotechnology, in particular to a device for carrying out manipulations on the reconstruction of the embryo.A device for dividing embryos, including the pipette when the covered ZP and micropipette for the expulsion of the embryos from the shell [the book. "Embryo transfer and genetic engineering in livestock". A. C. kvasnitsky etc. - Kiev, "Harvest", 1980, S. 167 - prototype] . When working with device requires high qualification of the operator, and a great time.The known device in connection with the need for high skill of the operator and the great complexity of working with him when dividing embryos do not permit this operation is required for agricultural production volumes. You need a device which is simple, convenient and provide a minimal investment of time.This technical result is achieved in that the device for dividing embryos, including a cutting element and micropipette fitted sleeve with a concave hemispherical socket, and a cylindrical hole, and placed in the sleeve can move it with a rectangular frame. Micropipette made in the form of semi-cylinders with pistons and rods. In addition, micropipette equipped with stops and merged flat sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve, with the formation of the cylinder inner wall. The upper ends of the flat sides of micropipets m Fulci is cutting element, and under the lower ends of micropipets rods which, by means of detachable locks mounted on the rectangular frame - feed screw.The drawing shows a device for dividing embryos, including two semi-cylindrical micropipette 1 being folded flat sides form a cylinder with an internal partition. The upper ends of micropipets 1 provided with lugs 2 and bonded to each other through their input in the Central cylindrical hole of the fixing sleeve 3 with a concave hemispherical socket 4, located above the ends of the inserted micropipettor 1. The upper ends of the flat sides of micropipets 1 made in the form of concave semicircle. In the sleeve 3 can move fixed, movable frame 5 made in the form of a rectangle, one side of which (over the nest 4) fixed removable cutting element 6 with an adjusting screw 7, is rigidly connected with the base of the cutting element and providing the required distance from the edge of the cutting element to the embryo when setting up the device. On the opposite side of the frame 5 is fixed by a screw connection of the feed screw 6, which interacts with the lower ends of micropipets 1, in which the mouth is a device may be fastened by a screw connection screw 8 in the stand 12, providing free access to the screw head 8. The embryo 13 is located in the cavity of the upper ends of micropipets 1, based on their (micropipettes) dual flat sides. The Assembly of the device is performed as follows: two micropipette 1, United flat sides, are introduced into the fixing hole of the sleeve 3 to the stop 2, while the piston 9 micropipettor 1 are in the top position, and releasable locks 11 of the rod 10 is fixed on the frame 5, the feed screw 8 when it is screwed all the way into the lower ends of micropipets 1. After Assembly, the device can be fixed by fixing sleeve 3 in the tripod and secure on the stand 12 of the screw. 8. In the first case, the design of the development should provide a sufficient coefficient of friction between the bushing and frame, and in the second case, the screw connection 8 with stand 12 should be a screw. The whole device is operable in a manual version, i.e. without pinning. In this case, the operator holds the assembled device for micropipette, lowers the embryo into the slot 4 and powercivil screw 8. Frame 5 slides over the screw 8 down together with the cutting element, cutting the embryo.Adjustment of the cutting elem: while in the upper cavity of micropipets 1, the embryo 13 being in the shell and based on twin-selected in the form of a semicircular flat side of micropipets 1, ready for operation. The cutting element 6 by means of a screw 7, rigidly connected to the base of the cutting element is lowered and fixed in the immediate vicinity of the shell of the embryo 13. The need to adjust the position of the cutting element may occur after leveling (grinding) or replacement of the cutting element. The adjustment is done under a microscope. Assembled and adjusted, the device is ready to work.The device operates as follows. The embryo 13 with a drop of medium, placed in a hemispherical socket 4 is rolled into a recess (cavity) over the ends of micropipets 1. Screwing the feed screw 8, placing the movable frame 5 in the lower position, fixed on the frame 5, the rod 10 of the piston 9 is moved together with the frame 5 down, creating a vacuum in the cavities of micropipets 1 under the embryo 13. Fixed embryo 13 dissected cutting element 6, since the movement of the frame 5 down cutting element 6 under its influence also moves down. Half of the embryo 13 are sucked into the micropipette. For removal of micropipets 1 detachable locks 11 are exempt from semiplane with movable frame 5, the Ute and conduct further manipulation, associated with quality assessment, cultivation, transplanting, and so on,Next is the Assembly of the device with new or vacated used micropipette and is the process of dividing the next embryo.Thus, this device allows you to simplify the process of division of the embryo so that it does not depend on skill of the operator.This simplicity and the minimum time of the entire process of dividing allow you to use this device in an industrial environment.
ClaimsDevice for dividing embryos, including a cutting element and a micropipette, characterized in that it is provided with a sleeve with a concave hemispherical socket and a cylindrical bore and placed in the sleeve can move it with a rectangular frame, and micropipette made in the form of semi-cylinders with pistons and rods and provided with stops, United plane sides by entering their upper ends to lugs and locks into the cylindrical hole of the sleeve to form a cylinder with an inner wall, the upper ends of the flat sides of micropipettes made in the form of concave semicircle, in moving pramogos, the rods which, by means of detachable locks mounted on the rectangular frame - feed screw.
FIELD: medicine; medical engineering.
SUBSTANCE: device has casing having an opening for introducing culturing medium, one or several ovocytes and sperm with following embryo(s) taking away using removal catheter and self-sealing closure means for selectively opening or closing the opening in container casing. The casing has basic chamber for placing culturing medium, ovocytes and sperm therein and micro-chamber for collecting and following taking out one or several embryos. The casing has means for restricting access to the micro-chamber with removal catheter or pipette. The micro-chamber is connected to the main chamber. Method involves preparing container, filling the container with culturing medium and introducing one or several ovocytes and sperm into the culturing medium, closing the opening, incubating the closed container for fertilizing and cultivating ovocytes, creating conditions favorable for produced embryo(s) migrating into the micro-chamber and taking one or several embryo(s) from the micro-chamber using catheter or pipette.
EFFECT: simplified method.
22 cl, 15 dwg
FIELD: medicine, embryology, in particular isolation of inner cell mass to produce human embryonic stem cell (hESC) lines.
SUBSTANCE: claimed method includes step of immobilization of embryo having pellucid zone, trophoblast and inner cell mass on blastocyst stage; providing of opening in embryo on blastocyst stage by using laser ablation; and removing of inner cell mass through said opening. Opening is crosses through pellucid zone and trophoblast. Laser ablation is carried out using contactless diode laser. Inner cell mass, isolated from embryo on blastocyst stage is useful in production of human embryonic stem cell lines.
EFFECT: simplified method for isolation of inner cell mass.
30 cl, 2 ex
SUBSTANCE: invention relates to medicine, in particular to gynecology, and deals with extracorporal fertilisation. After induction of superovulation for puncture of follicle into vagina cavity introduced is heated to body temperature tubular element with ball-like tip, which has guide canal on axis for puncture-biopsy needle. By means of it respective side of posterior vaginal fornix is pressed into abdominal cavity for maximally possible distance. Ovary is moved with side of mature follicles to contact of selected mature follicle with posterior fornix wall. Follicle centre is superposed with axis of tubular element canal, follicle is fixed in said state, distance from place of puncture of vaginal mucous membrane from the side of its cavity to follicle centre is determined and needle is moved out of the canal for said distance. After that follicular fluid is aspirated.
EFFECT: method ensures targeted puncturing of follicle centre, which results in reduction of traumaticity and increase of extracorporal fertilisation efficiency.
SUBSTANCE: group of inventions relates to medicine and can be used for extraction, in particular oocyte, from organism of human patient or animal patient. Vaginal needle for oocyte extraction includes first tubular section, which is in hydrodynamic connection with second tubular section. First tubular section has guide end for penetration into patient's tissue, and second tubular section, which includes tracking end for hydrodynamic connection with means of fluid reception. First tubular section has external diameter, which is smaller than external diameter of second tubular section, and first tubular section has internal diameter, which is smaller than internal diameter of second tubular section. Second tubular section has length for passing vagina and reaching tissue with oocyte. First tubular section has lengthsuitable in application for penetration into said tissue for reaching oocyte, but without penetration of second tubular section into said tissue. Method of taking sample from patient's organism includes introduction of needle for oocyte extraction into area, from which sample is to be extracted, and extraction of sample from patient's organism.
EFFECT: it is possible to extract sample which is located in area in patient's organism which is difficult to access.
26 cl, 15 dwg
SUBSTANCE: patient's vaginal mucosa and cervix are treated with antiseptic solution 1-2 days before transfer in preparation for transfer of frozen thawed embryos at blastocyst or morula stage or in transfer of fresh embryos at blastocyst or morula stage. Mucus is removed from cervical canal. Not less than 500 international units of human chorionic gonadotropin and not less than 30 international units of granulocyte macrophage colony-stimulating factor are introduced into uterus cavity by means of catheter; with transfer of embryos into uterus cavity being carried out 1-2 days after said procedure.
EFFECT: method makes it possible to increase pregnancy rate in patients undergoing treatment from infertility, suffering from intrauterine pathology and impairment of endometrium receptivity, provide possibility of reducing quantity of transferred embryos, reduce number of gestation complications, and improve health indicators of newborns.
SUBSTANCE: invention relates to medicine, particularly to obstetrics and gynaecology, and can be used for embryo transfer (ET) after extracorporeal fertilisation (EF). For this purpose, method includes forming 2 cohorts of oocytes, zygotes and embryos, originating from right and left ovary. In case of successful cultivation and development of at least 2 prime quality embryos (PQE) to obtain singleton pregnancy PQE is transferred from one cohort. To produce dizygotic pregnancy, method includes transfer of at least 2 PQE from different cohorts.
EFFECT: method enables to control onset of singleton or multi-zygotic (dizygotic) pregnancies in ET and EF programs.
1 cl, 1 tbl, 7 ex
SUBSTANCE: to determine in vitro promising embryos for subsequent implantation into the uterus during the procedure of in vitro fertilization (IVF) by means of the time-lapse video recording in 2-cell embryos, the sequence and orientation of the planes of division (horizontal/equatorial E, vertical/meridional-M) of each blastomer. It is taken into account that such planes can be: successive equatorial (EE), successive meridional (MM), meridional, then equatorial (ME), equatorial, then meridional (EM). And if the sequences of divisions in these planes correspond to the variants (EM) or (MM), then such embryos are considered promising for subsequent implantation into the uterus during the IVF procedure.
EFFECT: early detection and transfer of the most promising embryos for implantation, which increases the frequency of implantation and pregnancy.
2 dwg, 2 tbl, 2 ex
SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.
EFFECT: enhanced effectiveness in producing living descendants.
39 cl, 5 dwg, 1 tbl
SUBSTANCE: the present innovation deals with individual matching donor sheep to recipient sheep at similar antigenic composition of blood types being correspondent to the value of antigenic similarity index being ra=0.51-1.00, where ra - antigenic similarity index. Moreover, the mentioned antigenic similarity index should be calculated by the following formula: where S - the number of similar antigens in a donor sheep and in a recipient sheep, n1 - the number of detected antigens in a donor sheep, n2 - the number of detected antigens in a recipient sheep. The present innovation enables to increase the level of adaptability of transferred ovine embryos by 25%.
EFFECT: higher efficiency of embryo transfer.
3 ex, 4 tbl