Therapeutic and prophylactic leaf linden folia tilia and the method for the extractives

 

The invention relates to medicine, namely to homeopathic, treatment and prevention tools for internal and external use, has tonic, anti-inflammatory, wound healing, detoxifying, anti-microbial, antianginal, anti-atherosclerotic, antioxidant, antihypoxic, neuroprotective, sedative, cardioprotective, anti-arrhythmic, angioprotective, stress-protective, immunotropic, antifungal, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, megaproducer, radioprotective, and retinoprotective anticoordination action. The tool is an aqueous extract from leaves harvested in phase from early Bud to leaf fall, in the following ratio, wt. %: extractives of 0.1-47, 10-95% ethyl alcohol and purified water rest. The extraction is carried out first with water, then 15-96% ethyl alcohol at 40-90oWith the method of fractional maceration or percolation within 1-168 h at a ratio of raw materials-extractant 1:(5-100), resulting extract combine and allocate the amount of extractives. The invention allows realitv is, namely the creation of pharmacological agents of broad-spectrum plant-based raw materials and the method of its production, namely, therapeutic and preventive tool for internal and external use with tonic, anti-inflammatory, wound healing, analgesic, detoxatrim, antimicrobial, antianginal, anti-atherosclerotic, antioxidant, antihypoxic, neuroprotective, sedative, cardioprotective, anti-arrhythmic, angioprotective, stress-protective, immunotropic, antifungal, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, megaproducer, radioprotective, and retinoprotective anticoordination effect and to a method of making funds on the basis of extractives of lime leaves Folia Tilia, harvested in phase from early Bud to leaf used to increase the protective properties of the body, disorders of the liver, urinary system, chronic, indolent processes of the gastrointestinal tract, musculoskeletal system, diseases of the upper respiratory tract, organs blood, Central nervous system,od.

A genus of trees of the family of lime (Tilia) has about fifty species in the Northern hemisphere and about seventeen species of trees growing in Russia and in CIS countries [2].

There are a large number of pharmacological preparations of vegetable raw materials lime with therapeutic-preventive effect mainly from the flowers of the Linden, including various ways of obtaining them, but most of them have a small range of pharmacological activity and are used for the prevention and treatment of specific diseases, which significantly limits their use[1, 7, 8, 9].

Know the use for medicinal purposes plant materials - buds, leaves and flowers with bracts Linden heart. In folk medicine chopped fresh buds and fresh leaves are used as external analgesic and anti-inflammatory agents. In addition, it is known the use of this raw material for the treatment of venous ulcers and wounds, to treat hemorrhoids, rheumatism and gout, as a sedative in migraine, Linden flowers are used as a sedative, anticonvulsant and analgesic agent. In official medicine they use the on-prophylactic, namely alcohol extract of the plant material, which includes the flowers of the Linden, the product has detoxifying, adaptogenic, anti-inflammatory action.

This tool requires a significant set of herbs, and has a limited pharmacological spectrum of activity [10].

It is known for the treatment of diabetes, representing the emulsion on the basis of extractive substances from the mixture of plant material, which consists of lime flowers, heart [11].

Known means, containing an extract from the powdered bark of the Linden, and its production method [12] . Anti-inflammatory, wound healing, antibacterial, detoxifying agent is a water extract obtained by extracting the crushed bark of the Linden. The extraction was carried out for 98 hours at a temperature of 95oWhen the ratio of water, plant material and urea 1:0,4:0,08. To obtain dry extract liquid fraction is dried at a temperature of 60oC.

The disadvantage of the drug is not sufficiently wide spectrum of pharmacological activity and the presence of negative side effects, while using bark of Linden for preparation detrimental effect on the condition the rum was described therapeutic agent from the leaves of the Linden Folia Tilia with a broad spectrum of activity and method of its production, thus the proposed means of leaf Linden Folia Tilia and method thereof do not have a prototype.

The objective of the invention (the required technical result achieved when using the invention is the creation of new therapeutic and preventive tool with a wide range of actions which do not have contraindications to the use, and it has tonic, anti-inflammatory, wound healing, analgesic, detoxatrim, antimicrobial antianginal, anti-atherosclerotic, antioxidant, antihypoxic, neuroprotective, sedative, cardioprotective, anti-arrhythmic, angioprotective, stress-protective, immunotropic, antifungal, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, megaproducer, radioprotective, and retinoprotective anticoordination action as well as the development of the technology specified funds on the basis of extractives from leaves of Linden Folia Tilia used to increase the protective properties of the organism in the disruption of the liver, urinary and musculoskeletal system, chronic processes in the gastro-intestinal the Pnom diabetes, and also for the prevention of influenza and acute respiratory diseases in autumn and spring.

The tasks are solved and the required technical result is achieved in that therapeutic agent based on the extractive substances of vegetable raw materials lime according to the invention is a water and alcohol extract of extractives of lime leaves Folia Tilia prepared in phase from early Bud to leaf fall, in the following ratio, wt.%: Extractives including: flavonoid compounds, phenolic acids, amino acids, saponins, coumarins, tannins, polysaccharides, and 0.1 - 47 10-95% ethyl Alcohol and water as the Rest of the tool primarily contains 40% alcohol removing extractives of lime leaves cordate Folia Tilia cordata Mill prepared in phase from the beginning stage to the end of fruiting.

The task and the required technical result is also achieved by the fact that by way of obtaining funds, has tonic, anti-inflammatory, wound healing, detoxifying, anti-microbial, antianginal, anti-atherosclerotic, anti-oxidant, antigipoksicheskim, immunotropic, antifungal, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, megaproducer, radioprotective, retinoprotective, anticoordination action, according to the invention as plant materials used Linden leaves Folia Tilia prepared in phase from early Bud to leaf fall, and the extraction is carried out successively with purified water and 15-96% ethyl alcohol by the method of fractional maceration at a temperature of 40-90oWith or percolation at room temperature for 1-168 hours when sootnoshenii raw material:solvent 1:(5-100), the resulting aqueous extract is filtered and concentrated and combined with the filtered alcohol extraction, the combined water and alcohol extraction defend followed by separation of the amount of extractives. Mainly the extraction of lime leaves cordate Folia Tilia cordata Mill prepared in phase from early stage to late fruiting, carried out successively with water and 40% ethanol at a temperature of 90oMethod three maceration or percolation at 20oWith successively purified water and 40% alcohol, this volume, delanie amount of extractives from the United water and alcohol extraction carried out with the concentration of the extract up to 5-30% of airborne solids and drying the concentrate to obtain a dry extract.

The proposed group of inventions combined to form a single inventive concept, the second invention is intended to obtain the first. Both inventions solve the same task with achieving the same technical result.

It is found experimentally that as a result of this, claimed as the invention of the technology is possible in a maximum degree of extraction of biologically active substances (BAS).

A characteristic feature izobreteniya is the use of lime leaves Folia Tilia prepared in phase from early Bud to leaf fall, mainly as a vegetable raw material use Linden leaves cordate Folia Tilia cordata Mill prepared in phase from early stage to late fruiting, used fresh or dried leaves with petioles.

It was established experimentally that the accumulation of BAS Linden leaves grows from Bud break to early fall, with the largest accumulation of BAS proshlimi presented in table 1).

In addition, it is found experimentally that the Linden leaves do not have the irritating and sensitizing effect, and products based on the characteristic nespecificnomu action, low toxicity. They have a unique set of properties and virtually no contraindications. (The results of the pharmacological characteristics of drugs from the leaves of the Linden presented in table 4).

As follows from the above review of the prior art, the claimed therapeutic agent from the leaves of the Linden method thereof are not derived explicitly from known modern art, i.e., the proposed solutions are not obvious to a person skilled and inventive.

The invention is illustrated below by examples of implementation of the invention in Saint-Petersburg chemical-pharmaceutical Academy.

The Linden leaves cordate Folia Tilia cordata Mill prepared in phase from early stage to late fruiting, in the form of whole and ground leaves, on the edge dentate, upper side dark green, and the bottom - bluish color, with the content of extractives not less than 3%, yellowed and blackened leaves not more than 5%, other parts of rastenii crusher, sift through a sieve 2 and 3.

Liquid extractant different concentrations are prepared in a reactor with a stirrer. In the reactor serves 96% ethyl alcohol and there served in the required amount of purified water.

Example 1.

The Linden leaves cordate Folia Tilia cordata Mill in the amount of 10 kg load in percolator and fill in 75 l of extractant (purified water), carry out the extraction process (percolation) in the first hour, then the resulting aqueous extract of extractives is drained into the sump, then concentrated to one third of its original volume. Then this raw material is poured 75 l of extractant (mostly 40% ethyl alcohol), the extraction process is conducted similarly. Condensed filtered water removing extractives mixed with alcohol. The combined aqueous and alcoholic extract of extractives defend at a temperature of 5oWith in 24 hours or at a temperature of 15oC for 120 hours. The result is a therapeutic tool, which is a tincture brown, bitter taste with a sense of slizistosti, peculiar smell, containing 47% extractives (23% extractives in 40% alcohol etroite or content of 20% extractives in 10% ethyl alcohol).

Example 2.

The Linden leaves cordate Folia Tilia cordata Mill in the amount of 10 kg load in percolator and fill in 500 l of extractant (purified water), carry out the extraction process (percussie) during the first hour, then the resulting aqueous extract of extractives is drained into the sump, then concentrated to one third of its original volume. Then this raw material is poured 500 l of extractant (mostly 40% ethyl alcohol), the extraction process is conducted similarly. Condensed filtered water removing extractives mixed with alcohol. The combined aqueous and alcoholic extract of extractives defend at a temperature of 5oWith in 24 hours or at a temperature of 15oWith over 120 hours In the receive therapeutic and prophylactic agent representing a homeopathic tincture brown, bitter taste with a sense of slizistosti, peculiar smell, containing 0.1% extractives.

Example 3.

The Linden leaves cordate Folia Tilia cordata Mill in the amount of 10 kg load in the extractor with the water jacket and fill in 25 l of extractant (purified water) and the extraction is carried out (infusion) for 10 h, then the resulting odnosterzhnevaya (maceration) of treated water carried out three times at a temperature of 90oC. Straining carried out on a vacuum filter. Then this raw material is poured 25 l of extractant (40% ethyl alcohol) and similarly spend triple the maceration. The entire extraction process is 168 hours. First, the water extract is evaporated to one third of its original volume and mixed with alcohol. Therapeutic and prophylactic (alcohol extract) is a brown liquid, bitter taste with a sense of slizistosti, peculiar smell, containing 47% extractives.

Example 4.

Allocation of the amount of extractives produced from combined water and alcohol recovery obtained by the method of fractional maceration or percolation concentration using a vacuum distillation process, and then the concentrate is dried in a vacuum spray dryer to obtain a dry extract. The resulting powder from the leaves of the Linden Folia Tilia cordata Mill can be used for the manufacture of tablets, ointments, pastes, creams, suppositories, solutions.

Example 5.

Our research phytochemical composition proved that for therapeutic and prophylactic substances from the leaves of the lime can be characterized by the presence takihi, tannins, polysaccharides. For the determination of flavonoids conducted a qualitative color reaction (reaction with aluminium chloride) means add 1% alcoholic solution of aluminum-chloride in 95% ethyl alcohol, appears yellow-green staining, namely in the tool contains flavonoids.

Example 6.

Identification means for a group of biologically active substances like tannins, performed with the use of the ability to give a color reaction with ferric salts. The tool adds solution gentoomaniac alum, appears black and green coloring.

Example 7.

To prove the authenticity means a group of biologically active substances, such as polysaccharides, carried out the reaction of deposition of 95% ethanol. Appear flaky clots precipitated upon standing.

Example 8.

Identification of coumarins contained in the tool, perform chromatography, on the chromatogram see two spots of yellow.

Pharmacological study of treatment-and-prophylactic means on the basis of lime leaves Folia Tilia.

Studies of therapeutic and preventive tool for all types of pharmacological effects (acute and chronic toxicity, cumulative effects, aubrietia (see table.1, 2, 3, 4), that allowed us to test the tools on volunteers. Tests conducted more than 100 volunteers. Daily for 2 months each of them took 300 mg of dry extract (100 mg three traza a day after meals), every 10 days studied blood, urine, registered General condition. We observed no side effects. 38 volunteers, regularly carrying out physical activity of moderate intensity, improved, indicating the favorable impact of tools on the oxygen supply of the cells. 26 volunteers with various forms of vegetative-vascular disorders in the first two weeks of taking the means stabilized blood pressure and stopped the headaches. All volunteers over the age of 50 years reported improvement of vision, hearing, health, improved alertness, and memory. In the period of receiving funds from the children 10-15 years no cases of colds, revealed a significant improvement in immunological parameters (vosstanovlenie normal levels of immunoglobulins A, G, M and indicators of T-cell immunity).

The above detailed description of examples of the practical implementation of the group's image is surveillance technology have confirmed the consistent achievement of objectives. Created a new therapeutic tool based on leaf Linden Folia Tilia has tonic, anti-inflammatory, wound healing, detoxifying, anti-microbial, antianginal, anti-atherosclerotic, antioxidant, antihypoxic, neuroprotective, sedative, cardioprotective, anti-arrhythmic, angioprotective, stress-protective, immunotropic, antifungal, antibacterial, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, roprotective, radioprotective, retinoprotective, anticoordination effect and can be used to enhance the protective properties of the body, disorders of the liver, urinary system, chronic processes of the gastrointestinal tract, musculoskeletal system, in diseases of the upper respiratory tract, organs blood, Central nervous system, diabetes, as well as for the prevention of influenza and acute respiratory diseases in autumn and spring.

In General, given the novelty and non-obviousness of the invention, shown in section, of the invention, the materiality of all public and private signs image is the usefulness of" practicability of the invention and the achievement of invention tasks, the claimed group of inventions meets all the requirements of the inventions.

REFERENCES 1. Plant resources of Russia and adjacent countries: Part I - Family Family - Ephdraceae, part II - Supplement to 1-7-mu volumes. SPb.: World and family. - 1995, 96, - 571 S.

2. Plant resources of the USSR: Flowering plants, their chemical composition and use; family Paeoniaceae - Thymelanaceae. - L.: Nauka, 1985, - s.

3. State Pharmacopoeia of the USSR XI ed. - M.: Medicine, 1989, vol.2 - 400C.

4. The state register of medicines. - M., 2000, - 1202 S.

5. On the main page. Drugs in Russia, ed. 7th revised and enlarged more. - Reference. M: Attraversare. - 2001, - 1536.

6. Ant I. A., the Technology of medicines M., Medicine, 1980, page 186, 198, 202.

7. The Pastushenkov". L. C. , Lesiovskaja E. E. Plant - antihypoxants (phytotherapy). - SPb.: Chem.-Pharm. inst., 1991. - S. 26.

8. Popov A. P. Medicinal plants in folk medicine. - Kiev: Health, 1969, - 315 S.

9. The Pastushenkov " L. C. "Plant - friends of health". Lenizdat, 1989, page 84.

10. Patent RU 2085580, a 61 K 35/78, published 27.07.97.

11. Patent RU 2070409, A 61 K 35/78, published 20.12.96.

12. Patent RU 2038093, A 61 K 35/78, published 13.07.93.

Claims

3. The method of obtaining funds, has tonic, anti-inflammatory, wound healing, detoxifying, analgesic, antimicrobial, antianginal, anti-atherosclerotic, anti-oxidant, antigipoksicheskim, immunotropic, antifungal, nephroprotective, hepatoprotective, gastroprotective, thermoprotector, lipoic, megaproducer, radioprotective, retinoprotective, antisentimental action, characterized by the fact that as the plant materials used chopped fresh or dried leaves of Linden Folia Tilia prepared in phase from early Bud to leaf fall, and the extraction is carried out successively with water and purified 15-96% ethyl alcohol at a temperature of 40-90oWith the method of fractional maceration or percolation at room temperature for 1-168 h at a ratio of raw materials-extractant 1:(5-100), the resulting aqueous extract is filtered, concentrated and combined with the filtered alcohol extraction, the combined water and alcohol extraction defend followed by separation of the amount of extractives.

4. The method according to p. 3, characterized in that the extraction of lime leaves cordate Folia Tilia cordata Mill prepared in phase from the beginning stage to the end of the fruiting perform primarily successively with water and 40% ethanol by the method of triple maceration at 90oWith or percolation posledovatelnostei water and alcohol extraction defend at a temperature of 5-15oFor 24-120 h in the dark.

6. The method according to p. 5, characterized in that the selection of the amount of extractives from the United water and alcohol extraction carried out with the concentration of the extract up to 5-30% of airborne solids and drying the concentrate to obtain a dry extract.

 

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