Method of preparation of bioactive bone-plastic material "costme"

 

(57) Abstract:

The invention relates to medicine, namely to the harvesting of bone tissue, and may be applied in the "bone banks" to ensure bone-plastic material health care institutions. To produce bioactive bone-plastic material "Costme" plastic consistency with osteogenic properties, preventive antibacterial effect bone meal are combined in a ratio of 2:1 with a 9% solution of gelatin at 38-40oWith the addition of antibacterial agents, such as refline. Antibiotics added at a concentration corresponding to the optimal local antibiotic dose. The method allows to create the material, stimulating osteogenesis, profiling purulent complications. 1 C.p. f-crystals.

The invention relates to medicine, namely to the harvesting and preservation of bone tissue, and may be applied in the "bone banks" to ensure bone-plastic material health care institutions.

Known method of preparing bone meal bone-plastic surgery (Preservation and transplantation of tissues and organs. Edited, Kristinova. Sofia, 1975. the N. a method of obtaining a hemostatic, wound healing and osteoplastic means (Istrana L. P., Astranova E. C., Kurdyumov, S., and others, the patent of the Russian Federation N2091083), the main components of which are collagen, hydroxylapatite and the medicinal substance. In addition to the obvious advantages of this material has certain drawbacks, it includes hydroxylapatite ceramics obtained in the production process, and does not contain cellular elements and morphogenetic protein, which normally are found in the bone tissue.

The use of collagen as a liaison not always indifferent to the immune system as a possible manifestation of immune immunity on the part of the body.

The amount of medicinal substance (antiseptics) is up to 30% by weight of funds that exceeds the minimum required number several times and can hardly be justified.

The objective of the invention: obtaining bioactive bone-plastic material, plastic consistence, having osteogenic properties, preventive antibacterial effect.

Achieving these objectives will help to increase the final results of the bone and plastic surgery.


- strengthening of reparative processes in the damaged tissues;

- fast healing and restoration of bone structure;

- prevention of suppurative complications.

The positive effect of the proposed method for the preparation of biologically active bone-plastic material is achieved by the use of crushed bone (bone meal), which contains both organic and mineral components of bone tissue. Organic component contains proteins osteogenesis stimulators (protein gradients), and the mineral component is a hydroxyapatite of biological origin (i.e., depot minerals). As the link uses a 9% solution of gelatin (product of partial hydrolysis of collagen) in preservative fluid (11% sucrose solution, 0.9% NaCl, and others). It is important that the amino acid composition of gelatin refers to defective proteins and contains many residues of glycine, Proline, hydroxyproline, alanine (i.e., substances which take part in the building of new bone), which reduces the risk of immunological reactions. In addition, the biomaterial is injected antibiotic, for example, reflin (the product of the first order for hee is cha is solved by they use native bone allamuchy and the product of partial hydrolysis of the collagen - gelatin. Bone allamuchy are combined in a ratio of 2:1 with a 9% solution of gelatin on the preserving solution at 38-40oWith the addition of antibacterial agents, such as refline. Antibiotics, for example, reflin add in a dose corresponding local antibacterial.

The method is performed in the following way. Allogenic diaphyse tibial bone cut on a plate, in the process of sawing is formed bone flour with a particle size of 0.1-0.5 mm

To prepare galatarasay environment in a heat resistant flask containing a particular preservative, for example 11% solution of sucrose, gelatin is made from the calculation to get a 9% solution. Food gelatin is better to take the first or higher grade.

The number konserviruyushchie solution is determined by the volume of the prepared material. Leave for 1-1 .5 hours at room temperature before swelling of gelatin.

The flask placed in a water bath and heated until complete melting of gelatin. During heating it is necessary to make periodic stirring of the contents of the flask by means ot the Lin in a ratio of 0.5 mg to 10 ml of a 9% aqueous gel.

The next stage in the ratio of 2:1 (6 g flour 12 ml of a 9% gel=3 g gel) connect allamuchy with heladeria environment and cooled to room temperature. Packed in 0.25 and 0.5, Preserved by freezing at -20oWith or lyophilizer. Then sterilize with gaseous ethylene oxide or Rg-radiation.

1. Method of preparation of biologically active bone-plastic material by use of collagen of animal origin, characterized in that the use of native bone meal and the product of partial hydrolysis of the collagen - gelatin (9% solution), which are combined in a ratio of 2: 1 on the preserving solution at 38-40oWith the addition of antibacterial agents, such as refline.

2. The method according to p. 2, characterized in that antibacterial drugs, such as reflin add in a dose corresponding optimal local antibacterial.

 

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