Method of preparation of bioactive bone-plastic material "costme"
(57) Abstract:The invention relates to medicine, namely to the harvesting of bone tissue, and may be applied in the "bone banks" to ensure bone-plastic material health care institutions. To produce bioactive bone-plastic material "Costme" plastic consistency with osteogenic properties, preventive antibacterial effect bone meal are combined in a ratio of 2:1 with a 9% solution of gelatin at 38-40oWith the addition of antibacterial agents, such as refline. Antibiotics added at a concentration corresponding to the optimal local antibiotic dose. The method allows to create the material, stimulating osteogenesis, profiling purulent complications. 1 C.p. f-crystals. The invention relates to medicine, namely to the harvesting and preservation of bone tissue, and may be applied in the "bone banks" to ensure bone-plastic material health care institutions.Known method of preparing bone meal bone-plastic surgery (Preservation and transplantation of tissues and organs. Edited, Kristinova. Sofia, 1975. the N. a method of obtaining a hemostatic, wound healing and osteoplastic means (Istrana L. P., Astranova E. C., Kurdyumov, S., and others, the patent of the Russian Federation N2091083), the main components of which are collagen, hydroxylapatite and the medicinal substance. In addition to the obvious advantages of this material has certain drawbacks, it includes hydroxylapatite ceramics obtained in the production process, and does not contain cellular elements and morphogenetic protein, which normally are found in the bone tissue.The use of collagen as a liaison not always indifferent to the immune system as a possible manifestation of immune immunity on the part of the body.The amount of medicinal substance (antiseptics) is up to 30% by weight of funds that exceeds the minimum required number several times and can hardly be justified.The objective of the invention: obtaining bioactive bone-plastic material, plastic consistence, having osteogenic properties, preventive antibacterial effect.Achieving these objectives will help to increase the final results of the bone and plastic surgery.
- strengthening of reparative processes in the damaged tissues;
- fast healing and restoration of bone structure;
- prevention of suppurative complications.
FIELD: medicine, in particular bioactive complex for organogenesis.
SUBSTANCE: claimed complex represents multicomponent, bulk, three-dimensional structure, containing human allogene mesenchyme and epithelial cells and at least one layer of biocompatible polymer in form of collagen structure on network matrix. Biological complex of present invention is useful in regenerative and reparative reconstruction of any biological structures of mesemchyme-epothelial or mesodermal and ectodermal origin. Also disclosed are unified method for rebuilding of three-dimensional tissue defects and effective method for treatment of various gullet, urinary bladder parries, skin, gorge, eardrum, kidney, etc. defects. Complex also may be used in production of donor tissue equivalent bank.
EFFECT: active complexes for organogenesis useful in medicine.
3 ex, 1 dwg, 24 cl
SUBSTANCE: method involves applying hydroxyapatite collagen material plate so that plate size is selected to be by 5-8 mm greater than trepanation opening.
EFFECT: enhanced effectiveness in making plastic repair of maxillary sinus; excluded allergic responses.
SUBSTANCE: method involves introducing sharp tip of hollow needle into bone cortical layer crack. The crack is filled through needle lumen by introducing Collapan gel mixed with radiopaque substance in minimum concentration providing radio contrast range excess above injured bone contrast. Soft tissues are pierced with the needle in the vicinity of crack filling area center. Needle bevel plane is set in crack plane projection and introduced into the crack. Then, needle tube is rotated with its bevel plane directed into crack space. The cerebral covering soft tissues are pressed to bone along the crack path above positioned needle tip projection and the mixture is introduced in the amount equal to crack cavity region under pressed sift tissue. The needle end is pulled and directed towards the opposite crack region without removing it from the soft tissues and the region is also filled. Collapan introduction into crack fissure is carried out under X-ray optoelectronic transducer control.
EFFECT: enhanced effectiveness of treatment; reduced risk of postoperative complications.
FIELD: medicine, ophthalmology.
SUBSTANCE: the present innovation deals with a drainage for treating glaucoma that includes collagen types II-III immobilized in polymer of acrylic and vinyl rows, includes a cytostatic covalently bound with collagen types II-III, hypotensive preparation and not less than one rigidity rib at certain ratio of components. The innovation provides improved ocular hydrodynamics, suppression of reparative and cicatricial processes around the drainage suggested.
EFFECT: higher efficiency.
6 dwg, 3 ex
SUBSTANCE: the present innovation deals with the method to accelerate mucosal healing due to the following technique: one should apply a membrane consisted of purified collagenic material obtained out of natural collagen-containing tissue onto the part of affected mucosa to provide the chance for mucosal reconstruction in this part and, also, it deals with mucosa-regenerating preparation and application of purified collagenic material obtained out of collagen-containing natural tissue for preparing mucosa-regenerating preparation. The innovation provides more modified method that accelerates mucosal regeneration, as a whole, and, particularly, after surgical operations associated with the plasty of oral fornix.
EFFECT: higher efficiency.
12 cl, 3 dwg, 5 ex
SUBSTANCE: bone-and-mineral product contains porous bone mineral particles produced from natural bone and having crystalline structure practically corresponding to natural bone structure and practically containing no endogenous organic material. The particles have fibers of physiologically compatible type II resorbable collagen at least on their surface. Mass proportion of type II collagen fibers and porous bone mineral is at least equal to approximately 1:40.
EFFECT: enhanced effectiveness in recovering combined injuries of cartilage and bone tissue in articulations having defects.
8 cl, 6 dwg
SUBSTANCE: collagen tubes can be used for connection and regeneration of damaged nerves. Tube is formed of single sheet of collagen-containing material. Sheet has smooth small-sized external barrier surface preventing connecting tissue from growing through it. Tube also has internal sift fibrous surface which helps nerve to grow at higher speed.
EFFECT: higher speed of regeneration of damaged nerves' prevention of scars.
18 cl, 4 att, 4 dwg
SUBSTANCE: method of biomedical material production based on titanium and cobalt alloy in mode CBC includes as follows. Exothermal mixture of raw reagents made of powdered titanium and cobalt is prepared, added with max. 4 wt % titanium hydride and max. 15 wt % amorphous nanodisperse powdered calcium hydroxyapatite (CHA) or nanocomposite calcium hydroxyapatite with biopolymers. This powdered raw mixture is pressed to preparation blanks placed to reactor CBC. Preparation blank is preheated to temperature 350-580°C. Combustion action is initiated in inert atmosphere with following release of end product. Produced biomedical material represents mushy alloy of formula TiCo of total porosity 55-70% at open porosity portion 90-98% with prolate pores sized 200-800 mcm. Pore partitions are also porous with prevailing round pores of diameter ca 100 microns. Surface of mushy alloy is coated with calcium, phosphor and oxygen compounds which are hydroxyapatite decomposition products with prevailing content of calcium perovskite.
EFFECT: material is noncytotoxic, cells are characteristically split and actively migrate inside of mushy space.
4 cl, 4 dwg, 3 ex
SUBSTANCE: bone of natural origin is cleaned, sawed up to 0.2-2.0 cm thick plates, washed with heated to 65°C 0.1 M pH 5.8-6.0 phosphate buffer, digested in 0.1-0.4% activated papain solution at 65°C during 24 hours, then washed in five volumes of water at 40-80°C, treated with 0.4 N alkali at room temperature during 10-24 hours, rinsed in running water, degreased in ethanol/chloroform mixtures in ratio 1:2 firstly, and 2:1 secondly, decalcified in 0.4-1 N hydrochloric acid, treated with 1.5-3% hydrogen peroxide during 4 hours, washed with purified water, then with ethanol, dried at room temperature, packed up and sterilised. Material for osteoplasty and tissue engineering represents compound, in which native collagen matrix space structure and natural bone mineral component are preserved, containing 25% collagen and 75% mineral matter. According to dry material analysis it includes less than 1% non-collagen proteins.
EFFECT: method improvement.
3 cl, 5 ex
SUBSTANCE: group of inventions refers to pharmaceutics and medicine and concerns method of producing bioabsorbable matrix containing native nonreconstructible collagen type I, same bioabsorbable matrix used for filling defected soft and hard tissues, as implant, also method of treatment, reparation or replacement of tissue with using the disclosed bioabsorbable matrix bioabsorbable matrix. Method of producing the bioabsorbable matrix includes after treatment of animal skin; herewith derma treated with aqua-alkali solution containing sodium hydroxide, potassium dihydrogen phosphate and aqueous or anhydrous borax at temperature 1 to 10°C; herewith derma treated with aqueous solution of sodium sulphate and sodium hydroxide; herewith derma treated with aqueous solution of sodium sulphate; herewith derma treated with aqueous solution of boric acid. At the third fourth and fifth stages the derma is treated with periodic agitation of the solution periodically cooled to temperature 1 to 10°C; and each stage two to five includes water flushing of derma to ensure neutral pH of rinsing water.
EFFECT: invention ensures production of bioabsorbable matrix containing native nonreconstructible collagen type I with collagen fibre structure identical to natural collagen-containing tissue, possesses considerable mechanical properties and is applied as a matrix for growth, directional regeneration, improvement of soft and hard tissue trophism and structures.
32 cl, 1 ex, 3 dwg
FIELD: medicine, pharmacy.
SUBSTANCE: invention proposes implant prepared by mixing a carrier material with components of the preparation antibiotic/antibiotics with delayed release of an active substance (aminoglycoside, lincosamide antibiotics, 4-quinolone antibiotics and tetracyclines), and a method for preparing the implant. Release of an active substance from implant during from some days to some weeks doesn't dependent from a carrier material and adsorption effects of a carrier-material surface.
EFFECT: improved and valuable properties of preparation.
13 cl, 1 tbl, 6 ex
FIELD: medicine; therapeutic dentistry.
SUBSTANCE: new biological material for layer has low antigeny properties and keeps physical and mechanical properties of initial donor tissue. Biological laying has powder-like modified dentine with unblocked chemical bonds among collagen, chondrointin sulfates and mineral salts and Alloplant biological materials which have to be osteogeny stimulator, vasculogeny stimulator, phagocytosis stimulator at definite content of components. Laying for curing pulpitis has biological active matters to stimulate angiogenesis, dentinogenesis which matters activate phagocytosis.
EFFECT: improved efficiency of treatment.
1 dwg, 2 ex