The method of obtaining a medicinal product "squeaky"

 

The invention relates to medicine, namely to pharmaceutical technology get drugs from raw materials of animal origin. The method consists in the fact that the liver of the shark Katran homogenized, degrease by cold sludge in 1 - +5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.0 to 6.0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros and autoclave. The technical result is the stabilization of biologically active organo-preparation, the maximum recovery of cell SAP and quality of a medicinal product from shark liver Katran with a long shelf life for comprehensive methods of prevention, treatment and rehabilitation of various types of pathology of prostate cancer and as adjuvant in cancer practice. 1 Il.

The invention relates to pharmaceutical technology get drugs from raw materials of animal origin and can be used for complex methods of prevention, treatment and rehabilitation of various liquid extract biologically active water-soluble components, including the stage of grinding, homogenizing to a particle size of less than 500 μm, the centrifugation of the homogenate for separating particles from a liquid crude extract with subsequent separation, with the final liquid extract with a molecular weight components of the cartilage is less than or equal to 500 kilodaltons (KD) [see RF application 98110005 (PCT/SA 96/00549, PCT/WO 97/16197), CL IPC7A 61 K 35/60, publ. 20.06.2000,].

The disadvantage of this drug is a short shelf life, which ranks him among the elite drugs.

Closest to the claimed method is a method of producing a drug that has anticancer and analgesic activity, including homogenization of biosera, centrifugation and subsequent membrane filtration (see RF patent 2113224, IPC7A 61 K 35/12, 35/38, 35/407, publ. 20.06.98 year).

The disadvantages of the prototype is a short shelf life due to lack of stabilization.

The technical result of the invention is the creation of a quality of a medicinal product from shark liver Katran with a long shelf life (not less than 3 years) for complex methods of prevention, treatment and rehabilitation of various types of PV stabilization of biologically active organo-preparation and maximum extraction of cell SAP.

This technical result is achieved by the fact that in the known method of obtaining a medicinal product for the treatment of diseases of the prostate gland, which consists in homogenizing biosera, centrifugation, and subsequent membrane filtration according to the invention as biosera use shark liver Katran, which after homogenization degrease by cold sludge at 1-5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.06,0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros and autoclave.

This method will increase the shelf life and maximum extract of shark liver Katran cell SAP.

Cold sucks homogenate at a temperature below the 1oWith affects the process of separating solid and fat fractions from the homogenate, and at temperatures above 5oWith the process of samorazrusheniya fat (prothane), which dramatically reduces the medicinal properties of the drug and makes it unusable.

At the time of sediment less than 20 h nepo is the act of completely cleared.

Chemical coagulation carried out at pH<1,0 irrational, and at pH>6,0 worse medicinal properties of the drug and increases the pain of the injection.

Second sucks at room temperature exercise 5-6 h, which proved sufficient clearance from the film fats and residual proteins.

All modal parameters obtained experimentally.

The name "Squeaky" is a combination of the first four letters of the Latin words "Wells" - Squalus - shark and the "akan" - acanthias - edged, i.e. for drug use liver sharks edged, what is the species name black shark Katran.

An example of a specific implementation of the method.

For obtaining a medicinal product "Squeaky" use the black sea shark liver Katran, which is ground and the resulting minced add distilled water for the extraction of biologically active substances in aqueous solution.

For maximum extraction of cell SAP spend homogenization of the extract when the rotation speed of the rotary knife 6000 rpm for 5 min, followed by cold sucks at a temperature of +5oC for 22 h, homogenate section is practical coagulation proteins (stabilization) in the non-fatty extract is carried out until the temperature of the coagulation protein with subsequent acidification chloride-hydrogen acid extract and bringing the pH to 5.5 and 5.6, as the acidic environment contributes to the suppression of microbial growth and is the most physiologic, contributing less painful injection.

Second sucks at room temperature is carried out for 6 h, resulting in a top faction - coagulated protein (waste) and lower - stabilized extract.

Centrifugation stabilized extract is carried out at a rotor speed of 6000 rpm for 30 min followed by filtration of the supernatant through filter calico to free extract fat from the surface of the film.

Thin vacuum filtration produced through a membrane or capilano-membrane filter to obtain the extract (drug, "Squeaky") that meets the requirements of injection lekforma. A purified preparation "Squeaky" bottled in vials, sealed and subjected to autoclaving at an operating pressure of 1.2 ATM for 30 min with obtaining sterile injecting the drug. Mode autoclaving and time are set experimentally. Experimental verification showed that the shelf life is at least 2 years, and the storing for longer periods of time did not lead to precipitation.

0 patients voluntarily agreed to accept supportive therapy with Squatina". As a result, it was obtained the confirmation of the high adjuvant and antitoxic properties of the drug, "Squeaky significantly superior activity known and widely used in medical practice diseases of the prostate, bio-stimulants that can increase the effectiveness of treatment of diseases of the prostate, benign prostatic hyperplasia and chronic prostatitis. Increase therapeutic effect under the effect of the combined therapy with Squatina" lasts for 6 months of observation after the end of treatment and due to this therapeutic effect Squeaky" long and has a long life increased nonspecific resistance, high cell-mediated immunity and stress resistance. The introduction of Squatina" before surgery reduced postoperative complications and reduced the period of rehabilitation for 2-4 days compared to patients who were only on the basic therapy. On the basis of the conducted research "Squeaky" can be recommended for inclusion in the pathogenetic conservative complex t the completed order in the period of possible complications and prevent deterioration of urological symptoms of patients with chronic prostatitis and benign prostatic hyperplasia, be used in the surgical treatment of patients during preoperative preparation and postoperative rehabilitation.

The use of the drug, "Squeaky" in comprehensive prevention, treatment and rehabilitation of various types of pathology of the prostate, as well as adjuvant in cancer practice will allow for a comparison with the prototype to maximize the biologically active components of the cell juice shark liver Katran and increase the shelf life of the medicinal product due to their stabilization.

Claims

A method of obtaining a drug for the treatment of diseases of the prostate gland, which consists in homogenizing biosera, centrifugation, and subsequent membrane filtration, characterized in that as biosera use shark liver Katran, which after homogenization degrease by cold sludge with 1 to 5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.0 to 6.0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros, autoclave

 

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