The method of obtaining a medicinal product "squeaky"
The invention relates to medicine, namely to pharmaceutical technology get drugs from raw materials of animal origin. The method consists in the fact that the liver of the shark Katran homogenized, degrease by cold sludge in 1 - +5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.0 to 6.0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros and autoclave. The technical result is the stabilization of biologically active organo-preparation, the maximum recovery of cell SAP and quality of a medicinal product from shark liver Katran with a long shelf life for comprehensive methods of prevention, treatment and rehabilitation of various types of pathology of prostate cancer and as adjuvant in cancer practice. 1 Il. The invention relates to pharmaceutical technology get drugs from raw materials of animal origin and can be used for complex methods of prevention, treatment and rehabilitation of various liquid extract biologically active water-soluble components, including the stage of grinding, homogenizing to a particle size of less than 500 μm, the centrifugation of the homogenate for separating particles from a liquid crude extract with subsequent separation, with the final liquid extract with a molecular weight components of the cartilage is less than or equal to 500 kilodaltons (KD) [see RF application 98110005 (PCT/SA 96/00549, PCT/WO 97/16197), CL IPC7A 61 K 35/60, publ. 20.06.2000,].The disadvantage of this drug is a short shelf life, which ranks him among the elite drugs.Closest to the claimed method is a method of producing a drug that has anticancer and analgesic activity, including homogenization of biosera, centrifugation and subsequent membrane filtration (see RF patent 2113224, IPC7A 61 K 35/12, 35/38, 35/407, publ. 20.06.98 year).The disadvantages of the prototype is a short shelf life due to lack of stabilization.The technical result of the invention is the creation of a quality of a medicinal product from shark liver Katran with a long shelf life (not less than 3 years) for complex methods of prevention, treatment and rehabilitation of various types of PV stabilization of biologically active organo-preparation and maximum extraction of cell SAP.This technical result is achieved by the fact that in the known method of obtaining a medicinal product for the treatment of diseases of the prostate gland, which consists in homogenizing biosera, centrifugation, and subsequent membrane filtration according to the invention as biosera use shark liver Katran, which after homogenization degrease by cold sludge at 1-5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.06,0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros and autoclave.This method will increase the shelf life and maximum extract of shark liver Katran cell SAP.Cold sucks homogenate at a temperature below the 1oWith affects the process of separating solid and fat fractions from the homogenate, and at temperatures above 5oWith the process of samorazrusheniya fat (prothane), which dramatically reduces the medicinal properties of the drug and makes it unusable.At the time of sediment less than 20 h nepo is the act of completely cleared.Chemical coagulation carried out at pH<1,0 irrational, and at pH>6,0 worse medicinal properties of the drug and increases the pain of the injection.Second sucks at room temperature exercise 5-6 h, which proved sufficient clearance from the film fats and residual proteins.All modal parameters obtained experimentally.The name "Squeaky" is a combination of the first four letters of the Latin words "Wells" - Squalus - shark and the "akan" - acanthias - edged, i.e. for drug use liver sharks edged, what is the species name black shark Katran.An example of a specific implementation of the method.For obtaining a medicinal product "Squeaky" use the black sea shark liver Katran, which is ground and the resulting minced add distilled water for the extraction of biologically active substances in aqueous solution.For maximum extraction of cell SAP spend homogenization of the extract when the rotation speed of the rotary knife 6000 rpm for 5 min, followed by cold sucks at a temperature of +5oC for 22 h, homogenate section is practical coagulation proteins (stabilization) in the non-fatty extract is carried out until the temperature of the coagulation protein with subsequent acidification chloride-hydrogen acid extract and bringing the pH to 5.5 and 5.6, as the acidic environment contributes to the suppression of microbial growth and is the most physiologic, contributing less painful injection.Second sucks at room temperature is carried out for 6 h, resulting in a top faction - coagulated protein (waste) and lower - stabilized extract.Centrifugation stabilized extract is carried out at a rotor speed of 6000 rpm for 30 min followed by filtration of the supernatant through filter calico to free extract fat from the surface of the film.Thin vacuum filtration produced through a membrane or capilano-membrane filter to obtain the extract (drug, "Squeaky") that meets the requirements of injection lekforma. A purified preparation "Squeaky" bottled in vials, sealed and subjected to autoclaving at an operating pressure of 1.2 ATM for 30 min with obtaining sterile injecting the drug. Mode autoclaving and time are set experimentally. Experimental verification showed that the shelf life is at least 2 years, and the storing for longer periods of time did not lead to precipitation.
0 patients voluntarily agreed to accept supportive therapy with Squatina". As a result, it was obtained the confirmation of the high adjuvant and antitoxic properties of the drug, "Squeaky significantly superior activity known and widely used in medical practice diseases of the prostate, bio-stimulants that can increase the effectiveness of treatment of diseases of the prostate, benign prostatic hyperplasia and chronic prostatitis. Increase therapeutic effect under the effect of the combined therapy with Squatina" lasts for 6 months of observation after the end of treatment and due to this therapeutic effect Squeaky" long and has a long life increased nonspecific resistance, high cell-mediated immunity and stress resistance. The introduction of Squatina" before surgery reduced postoperative complications and reduced the period of rehabilitation for 2-4 days compared to patients who were only on the basic therapy. On the basis of the conducted research "Squeaky" can be recommended for inclusion in the pathogenetic conservative complex t the completed order in the period of possible complications and prevent deterioration of urological symptoms of patients with chronic prostatitis and benign prostatic hyperplasia, be used in the surgical treatment of patients during preoperative preparation and postoperative rehabilitation.The use of the drug, "Squeaky" in comprehensive prevention, treatment and rehabilitation of various types of pathology of the prostate, as well as adjuvant in cancer practice will allow for a comparison with the prototype to maximize the biologically active components of the cell juice shark liver Katran and increase the shelf life of the medicinal product due to their stabilization.
ClaimsA method of obtaining a drug for the treatment of diseases of the prostate gland, which consists in homogenizing biosera, centrifugation, and subsequent membrane filtration, characterized in that as biosera use shark liver Katran, which after homogenization degrease by cold sludge with 1 to 5oC for 20-24 h, and then fat-free homogenate direct thermal and chemical coagulation of proteins at pH of 1.0 to 6.0, defend at room temperature for 5-6 h with obtaining the stabilized extract after centrifugation and membrane filtration ampoliros, autoclave
FIELD: medicine, pharmaceutics.
SUBSTANCE: raw soft roe should be reduced, treated at certain temperature with 0.2%-acetic acid solution, dehydrated with alcohol, dried to obtain granules, granules should be extracted with sulfuric acid solution at three stages, proteins should be precipitated out of extract by adding triple volume of ethanol, residue should be dissolved, solution should be treated with barium hydroxide up to pH being 7.0-7.5 followed by addition of ammonia up to pH being 10.5-11.5 to separate the residue due to decanting and centrifuging. Solution obtained after decanting and centrifugate should be treated with kieselguhr at heating up to 80 C and filtered upon a Nutch filter, one should apply filtrate through cationite in "H+"-form, eluate should be concentrated due to vacuum evaporation or by applying baromembranous technique, then one should precipitate the product out of eluate with triple volume of alcohol due to a 5-fold reprecipitation along with centrifuging and drying the residue to remove residual moisture and dry ready-to-use product at 55-65 C for 24 h. The present innovation enables to increase the degree of purification of protamine sulfate due to purifying against accompanying foreign proteins.
EFFECT: increased anti-heparin activity.
FIELD: medicine, drug production.
SUBSTANCE: claimed method for production of heterogeneous deoxyribonucleic acid (DNA) sodium salt from animal male gonads and/or sperm thereof includes grinding and homogenization of starting raw materials in detergent solution, solution treatment at elevated temperature with simultaneous stirring, liquid phase separation by filtering and product precipitation with alcohol. Homogenization is carried out in 1-4 % sodium dodecyl sulfate solution in distilled water at 35-60°C with continuous stirring for 1.5-4 h followed by addition of coagulation agent, namely alkali or earth alkali metal permanganate to produce coagulation agent concentration in solution of 0.0005-0.03 M. Obtained solution is stirred at 35-60°C for 25-90 min, than ionic force is increased up to 1-3 M, solution is heated up to 60-62°C and stirred at abovementioned temperature for 1-3 h. Further contaminant precipitate obtained in coagulation process is separated by filtration thought filter having pore size from 1 to 25 mum. Filtered solution of non-fragmented (high molecular) DNA is cooled to 0-20°C, alcohol is added to obtain finished concentration of 27043.5 vol. %, Then obtained solution is agitated and deposited with alcohol preparation is filtered and separated, washed with 96 % ethanol and stored under ethanol. Obtained DNA preparation is dissolved in 0.01-4.5 M NaCl aqueous solution at 50°C or less; obtained solution is passed through high pressure homogenizer under pressure up to 2000 kg/cm2, obtained fragmented DNA is treated with alcohol wherein deposited with alcohol fermented sodium DNA is centrifuged and separated. Separated precipitate is rewashed with alcohol and dried in desiccator at 50°C or less to produce powder.
EFFECT: simplified technology for DNA sodium salt production with increased yield without losses of quality.
1 ex, 3 tbl
SUBSTANCE: claimed method includes utilization of acetic acid as cartilage eluating solvent. According the second embodiment method includes additional filtration of solution containing crude proteoglycan to remove precipitate from said solution. Then filtered solution is centrifuged, ethanol saturated with sodium chloride is added to obtained supernatant, and supernatant is centrifuged to concentrate abovementioned crude proteoglycan in precipitate. According the third embodiment method additionally includes dissolution of said precipitate with acetic acid. Proteoglycan obtained by method of present invention may bi administered perorally.
EFFECT: simplified and environmentally friendly method for proteoglycan production.
3 cl, 3 dwg, 1 ex, 2 tbl
FIELD: cosmetology, esthetic surgery, applied biopharmacology.
SUBSTANCE: invention relates to medicative and cosmetic agents, and uses of biologically active substances based on natural biological complexes. Claimed method includes application of Mirralgin balm having analgesic, anti-inflammation and resolving action, Reventon balm, having vessel restorative action, for edema reducing and microcirculation improving. In process of blepharoplastic eye boundary bandage microemulsion having recovery and animative action on eaves skin is applied, and in process of facelifting cream mask having bactericide, vessel restorative and recovering action, and enhancing of local immunity is applied. Mirralgin balm and Reventon balm treatment is carried out sequentially or alternatively. In another embodiment after skin cleaning and treatment of damaged zone with anti-septic agent Bacteriophage gel, which disinfects skin surface, prevents biofilm forming, accelerates cicatrizing process is applied, then posttraumatic zone is treated with cleaning milk, toned up with tonic lotion and cream mask having bactericide, vessel restorative and recovering action, and enhancing of local immunity is applied.
EFFECT: method for skin structure recovering without skin trauma.
4 cl, 7 ex, 1 tbl
SUBSTANCE: method involves administering Eucanol at a daily dose of 3-4 g and Ispradin at a daily dose of 1.2-1.3 mg twice a day. Eucanol is taken during a meal and Ispradin is taken 30-40 min before meals.
EFFECT: enhanced effectiveness of treatment; reduced drug consumption.
FIELD: medicine, obstetrics and perinatology.
SUBSTANCE: invention relates to a method for prophylaxis of the pregnancy gestosis. Method involves administration of aspiring beginning from the second trimester of pregnancy and additional administration of the preparation "Omeganol" in the dose 2 capsules, 3 times per a day for 1 month. Then 2 months later the course of administration of the preparation "Omeganol" is repeated. Method provides improving circulation in the system mother-placenta-fetus and anticoagulant and fibrinolytic effects that results to decreasing hypercoagulation that accompanies gestosis in pregnancy.
EFFECT: improved method of prophylaxis of gestosis.
3 tbl, 2 ex
FIELD: medicine, dermatovenerology.
SUBSTANCE: on establishing the diagnosis at the first stage of therapy it is necessary to prescribe derinate as 1.5%-solution per 5.0 ml intramuscularly 5 times every 48 h followed by magneto-infrared-laser impact: wave length being 0.85-0.95 mcm, impulse frequency being 50 Hz, power of light diodes radiation being 50 mW, magnetic induction corresponds to about 20-80 mTl, dosage of energetic impact ranges 0.8-1.07 J/sq. cm. Total time for procedure - 10 min. One should affect the area of thymus gland for 2 min, spleen - 2 min, inguinal lymph nodes - per 2 min, lumbosacral - 2 min. Therapy course consists of 10 daily procedures. The innovation enables to shorten therapy terms, improve quality of life in patients with urogenital herpes-viral infection in combination with chlamydiosis and increase the number of medicinal preparations for treating the above-mentioned combination of infections.
EFFECT: higher efficiency of therapy.
1 dwg, 1 ex, 2 tbl
FIELD: veterinary science.
SUBSTANCE: one should inject medicinal preparation for animals simultaneously externally and internally, moreover, as medicinal preparation it is necessary to apply antiseptic preparation based upon technical fish oil. The preparation mentioned should be applied twice or thrice daily onto animal's skin. At first, affected part of skin should be washed with preparation's solution at concentration of 1:4 - 1:5, correspondingly, antiseptic preparation : solvent with pulsing stream at alternating increased and decreased pressure. Then it is necessary to lubricate with the preparation in its pure form or with its solution at concentration being 1:1 up to 1:3, correspondingly, antiseptic preparation : solvent. Simultaneously, the preparation should be given for oral intake as a dietary constituent at 5 ml/100 kg body weight in animals. The innovation provides inhibition of microflora, the decrease of bacterial seeding of a wound and shortens therapy terms.
EFFECT: higher efficiency of complex therapy.
4 ex, 2 tbl
SUBSTANCE: method involves carrying out combined chemotherapy according to AC-scheme of adriamycin+cyclophosphane. Bioorganic preparation of animal origin is intramuscularly introduced in parallel to chemotherapy to a group of rats at a dose of 0.3 ml/100 g of body weight every day during 30 days.
EFFECT: enhanced effectiveness of treatment.
SUBSTANCE: method involves introducing bio-organic animal preparation in advance during 4 days at daily intramuscular dose of 0.3 per 100 g of animal mass, before introducing cancerogenic agent. Then, the cancerogenic agent injections are started and continued during 30 days.
EFFECT: increased tissular resistance, antitumor immunity and phagocytic macrophage activity.