The method for determining the functional state of the ovaries in farm animals

 

The invention relates to the field of veterinary medicine. Since 25-30-day postpartum period, examine the contents of total cholesterol and indicators of albumin-globulin ratio in the blood; then in animals with total cholesterol level of 5.3-7.5 mmol/l and having a value of albumin-globulin ratio in the range from 1.0 to 2.2, which show persistent immobility reflex, additionally determine pH and refractometric index of refraction of cervico-vaginal discharge and when the pH value to 5.0-6.8 and refraction the index of refraction of cervico-vaginal discharge, equal 1,3342-1,3352, by the content of the actual surface of cells with a distinct nucleus on the vaginal swabs to 7.5% diagnose the presence of functionally active follicle with Mature eggs in the ovaries caliraya cows and judged favorable conditions for fertilization. The method allows available in terms of livestock farms to diagnose the functional state of the ovaries in female farm animals, more accurately detect the hunt and ovulation in ruminants. 5 table.

The invention relates to the field of veterinary medicine, namely to Antonych animals in particular, can be used to identify the optimal time of insemination in caliraya cows in the postpartum period.

The method allows available in terms of livestock farms to diagnose the functional state of the ovaries in cows.

There is a way to determine the optimum time of insemination of females ruminants, which involves the study of a sample of cervico-vaginal secretions from the genital tract of cows using refractometry (Antonyuk B. C., garcin centuries, Y. Gorbunov A. Method of determining the optimal time of insemination in cattle. A. C. 1146036, USSR). Measurement of refraction index of refraction of cervico-vaginal secretion reflected light is judged optimal time of insemination of cows, which corresponds to a refractive index TACs equal 1,3351-1,3370.

There is a method of determining the functional state of the ovaries in animals with elongated vagina (Vasilenko I. F. Method for determining the functional state of the ovaries in animals with elongated vagina. RF patent 2155016), such as cows, which involves taking a swab from fernebukovoe fornix at the level of the cervix by removing the cell mass from the walls of the pipette on the Alo illuminated under visual control. Then samples a thin layer is applied on a glass slide, the cellular material is fixed, paint, differentiate and count different types of cells. To diagnose animals determine the percentage of the actual surface of cells with a distinct nucleus and "naked" nuclei and their "shadow". When the content of the actual surface of cells not more than 7.5% and "naked" nuclei and their "shadows" not more than 1.3% diagnose preovulatory the period of the sexual cycle and judged favorable conditions for insemination of cows. When the content of the actual surface of cells not more than 7.5% and "naked" nuclei and their "shadows" in the range of 3.1-8.5% of diagnosed pregnancies and judged favorable conditions for the development of the fetus. This method was taken as a prototype.

The main disadvantage of the previous methods is that the definition of the functional state of the ovaries in cows is based on studies of individual indicator only the state of the vaginal environment, i.e., these methods allow to identify preovulatory the period of sexual hunting, but not enough to fully determine the functional condition of the ovaries in cows.

The present invention is to develop a method with icebath functional condition of the ovaries of females of farm animals and to judge the presence of functionally Mature follicle in the ovaries of cows and therefore, to more accurately detect the hunt and ovulation in caliraya female ruminant animals (cows).

This is the technical result, which is in causal connection with the essential features of the invention.

The technical result of the method is achieved in such a way that starting from 25-30-day postpartum period is the sampling of blood from the jugular vein of cows three hours after taking their morning feed and examined the contents of total cholesterol and indicators of albumin-globulin ratio in the blood; then animals with high total cholesterol and having a value of albumin-globulin ratio of blood in the optimal range of values for this indicator are selected for further examination, selection of cows carried out in the presence of persistent immobility reflex in preovulatory period of the estrous cycle, when the animals allow themselves your batch of other cows in the herd, and during this period, perform the fence, cervico-vaginal secretions from the vagina and then examine the parameters characterizing the condition of the vaginal environment - pH and refractometric index of refraction of cervico-vaginal discharge in reflected light; although the interest content of different types of cells according to the prototype. Animals that have a total cholesterol level of 5.3-7.5 mmol/l and the values of albumin-globulin ratio of blood in the range of 1.0-2.2 and which preovulatory the period of sexual hunting show persistent immobility reflex and admit to yourself your batch of other cows in the herd are selected for further examination; diagnostic cows determine pH and refractometric index of cervico-vaginal discharge and calculate the content of superficial epithelial cells in vaginal smears; however, when the pH value of cervico-vaginal secretion at the level of 5.0-6.8 and refraction of the refractive index of cervico-vaginal secretion equal 1,3342-1,3352 and when the percentage of the actual surface of cells with a distinct nucleus on the vaginal swabs to 7.5%, diagnose the presence of functionally active follicle with Mature eggs in the ovaries caliraya cows.

The method is as follows: approximately 25-30-day postpartum period, taking blood samples from the jugular vein of cows three hours after taking their morning feed and determine the content of total cholesterol and indicators of albumin-globulin ratio in the blood; then animals with sa blood in the range from 1.0 to 2.2, and in the presence of persistent immobility reflex (animals allow themselves your batch of other cows in the herd) are selected for further surveys; preovulatory period of estrous cows from the vagina, using a pelvic mirror for animals with elongated vagina, collect cervico-vaginal secretion, which is collected in a glass tube with the lower branches of a mirror, then the tube is closed with a sealing cap and after 10-15 min in the laboratory are exploring the parameters characterizing the condition of the vaginal environment - pH using pH meter and refractometric index of refraction of cervico-vaginal discharge in reflected light on the Refractometer. At the same time carry out the capture of smears: a pipette for artificial insemination with processed rough end is inserted through the vaginal mirror with lights under visual control. Then samples a thin layer is applied on a glass slide, the cellular material is fixed, paint, differentiate and count different types of cells of the prototype. The optimal time of insemination (the presence of functionally active follicle with Mature eggs in the ovaries of cows) corresponds to the pH value of cervico-vaginalnogo the 3342-1,3352, and the percentage of the actual surface of cells with a distinct nucleus on the vaginal swabs to 7.5%.

Experiments to determine the optimal time estrous and ovulation in cows was conducted at the livestock farm Belgorodskoi scientific experimental biological station (WIBS), Komi scientific center, Ural branch of RAS (, Syktyvkar). Tested the proposed method was carried out on cows livestock farms farms "Suburban" and "Syktyvkar". Used high quality breeding cows Kholmogory breed with an average annual milk yield of 4000 kg and above in satisfactory conditions and feeding, walks in the paddock sites.

Because there is evidence of correlation between the timing of resumption of estrous activity in the ovaries of cows after birth and cholesterol content of the blood (Grummer, R. R., Carroll D. J. A review of lipoprotein cholesterol metabolism: importance to ovarian function// J. Anim. Sci. - 1988. - V. 66, 12. - P. 3160-3173; Kafidi N., Leroy R., Chapaux Ph., Iatasse L., Hanzen Ch. , Y. Laurent, Antoine 0. Etude de 1'influence de facteurs alimentaires sanitaires on d'exploitation sur la production laifiere et les performances de reproduction du betail laifier. Analyse statistigue// Ann. Med. Vet. - 1990. - V. 734, 35. - R. 83-91) and the interrelation between time normalization biorhythm estrous activity in cows and albumin is entrations in the blood of dairy cows in late pregnancy and early lactation and relationships with subsequent fertility// J. Agr. Sci. - 1980. - V. 94, 3. - R. 517-527), studies have been conducted content of total cholesterol and blood values of albumin-globulin ratio in cows during different periods of the reproductive cycle.

At the end of pregnancy and at certain intervals after birth in clinically healthy animals farm VIBS determined the content of total blood cholesterol. In the eighth, the ninth month of pregnancy and in the first month after birth in animals showed low concentrations of blood cholesterol within 3-4 mmol/l During the passage of the postpartum period in cows without obvious complications to the end of the first - the beginning of the second month after birth was celebrated the first manifestation of the estrous cycle, the content of cholesterol was increased and amounted to 5.4-6.2 mmol/l (table.1, subgroup 1). In animals with increased cholesterol content of blood was noted the resumption of the first astral cycle with normal sexual hunting - with obvious stage of proceedings at the beginning of the hunting and persistent immobility reflex in preovulatory the period at the end of the hunt.

Increased cholesterol in the blood of cows was also noted during the passage of ovulatory 'washing cycle immediately before oplodotvoreniia cholesterol values (p<0,001), that on average 27% lower quantities of cholesterol in the blood of cows with normal course of metabolic processes and the postpartum period (table.1 and 2, the second subgroup). In postnatal animals, having a cholesterol content of the blood is below the optimal values, noted the resumption of ovulatory estrous cycles later after birth, and therefore, they have lengthened the period from birth to fertilization (Vasilenko I. F. cholesterol in the blood Kholmogory cattle. In the book: Nutrient absorption in ruminants. Syktyvkar, 1987. C. 92-97. (Proceedings of the Komi branch of the USSR Academy of Sciences, 85).

Research performance of albumin-globulin ratio blood animal farm VIBS in preovulatory period of the estrous cycle before insemination and subsequent fertilization and in the first months of fruiting showed that these values should be within the optimal values for this indicator (table. 3). Therefore, the ability of cows to keep the value of albumin-globulin ratio of blood within the optimal values - 1,0-2,2 - is one of the conditions necessary for the normal functioning of the individual structures of the ovary in preovulatory period.

is azaela of refraction of cervico-vaginal discharge in reflected light in cows farm VIBS in preovulatory period of the estrous cycle during the insemination and subsequent fertilization showed what the number actually is the surface of cells lining the epithelium were in the range of 7.5%, and intermediate cells was less than 1.2%. The pH of cervico-vaginal discharge ranged from 5.0 to 6.8, and the refraction index in a more narrow borders - from 1,3342 to 1,3352 (PL.4).

Example. The proposed method for the determination of the functional state of the ovaries in farm animals with the study of total cholesterol and values of albumin-globulin ratio in the blood and indicators characterizing the state of the vaginal environment - pH, and refraction index of genital secretions and the percentage of surface epithelial cells in vaginal smears was tested on cows livestock farm complex "Syktyvkar". To control the correct selection of cows for artificial insemination surveys were conducted 7 animals in preovulatory period of the estrous cycle, when the animals showed persistent immobility reflex and allowed themselves your batch of other cows in the herd. Examined the content of total cholesterol, albumin-globulin ratio in the blood and simultaneous determination of pH and repakov (PL.5). The level of serum cholesterol was below normal in two animals 2 and 4. The value of albumin-globulin relationship was above normal in two animals 1 and 3, and one animal - 4 are significantly reduced compared to the optimal level. The pH and refractometric index corresponded to the optimal values in all 7 animals, while the cell smears from the cranial division of the vagina is not consistent with the norm in 6 cows, one of them expressed a form of inflammation (endometritis) diagnosed two. From 7 animals examined only one cow, all the studied parameters were within the range of values that are optimal for the functioning of the individual structures of the ovaries, and this animal were fertilized.

Taking into account the experimental results, we can conclude that the selection of cows with a high content of total blood cholesterol and the optimal values of albumin-globulin ratio of blood ranging from 25-30 days after birth, and manifesting persistent reflex immobility during the true estrous (preovulatory period of the estrous cycle), in conjunction with the optimum values of pH and refractometric the Telia on smears allows you to identify animals with functionally Mature follicles in the ovaries and with a high ability to fertilize. The study proposed parameters can also be used for accurate diagnosis of metabolic disorders or diseases of the reproductive system (endometritis) in cows in the early stages after birth.

Claims

The method for determining the functional state of the ovaries in farm animals, including the capture of smears from the walls of the upper lateral fornix, counting the percentage of certain types of superficial epithelial cells, and the judgment of the phases of the reproductive cycle and functional condition of the ovaries in cows, characterized in that the pre-starting with 25-30-day postpartum period, examine the contents of total cholesterol and indicators of albumin-globulin ratio blood, then in animals with total cholesterol level of 5.3-7.5 mmol/l and having a value of albumin-globulin ratio in the range from 1.0 to 2.2, showing persistent immobility reflex, additionally determine pH and refractometric index of refraction of cervico-vaginal secretions and Sachen, by the content of the actual surface of cells with a distinct nucleus on the vaginal swabs to 7.5% diagnose the presence of functionally active follicle with Mature eggs in the ovaries caliraya cows and judged favorable conditions for fertilization.

 

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