# The stereological method of determining the anisotropy of interest

The invention relates to the field stereological analysis of the spatial organization of objects, in particular when studying objects in their planar images. His use of light, transmission electron and confocal laser microscopy and computed x-ray, magnetic resonance and ultrasound imaging allows to obtain a technical result increased accuracy stereological analysis. This result is achieved due to the fact that the objects are subjected to interaction with the n-dimensional stereological probe, make measurement of the size of the output images of objects, the measurement results array in the form of the size distribution of the object images, which are approximated by setting the parameters of interest and stereological probe. 19 C.p. f-crystals, 3 tab., 4 Il. Description text in facsimile form (see graphic part)

Claims

1. The stereological method of determining the anisotropy of objects that have the shape described by geometric shapes, characterized in that the test object is subjected to interaction with the n-dimensional stereological probe, Proline size of the images of objects which are approximated by setting the parameters of interest and stereological probe.2. The method according to p. 1, characterized in that the distribution of the size of the images of objects are approximated by specifying any of the following, or any combination of the following: (a) distribution of forms of objects, b) the distribution of the size or sizes of objects in) the distribution of the angles describing the mutual orientation of the objects and stereological probes, g) distribution of the relative position of objects and stereological probes, d) distribution parameters stereological probes and (e) the distribution properties of the wave flow, used when projecting.3. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with nomernym (0D) stereological probe section.4. The method according to any of paragraphs. 1 and 2, characterized in that the objects are interacting with one-dimensional (1D) stereological probe section and asked, in the particular case of zero curvature.5. The method according to any of paragraphs. 1 and 2, characterized in that the objects are interacting with two-dimensional (2D) stereological probe section and asked, in the particular case of zero curvature.6. The method according to any of paragraphs. 1 and 2, otlichalis the particular case zero, the curvature.7. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with a combined n-dimensional stereological probe section and asked, in the particular case of zero curvature.8. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with stereological probe through the projection of objects on nullary (0D) stereological probe.9. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with stereological probe through the projection of objects on one-dimensional (1D) stereological probe with asked, in the particular case of zero curvature.10. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with stereological probe through the projection of objects on two-dimensional (2D) stereological probe with asked, in the particular case of zero curvature.11. The method according to any of paragraphs. 1 and 2, characterized in that the objects are subjected to interaction with stereological probe through the projection of objects in three-dimensional (3D) stereological probe with asked, in the particular case of zero curvature.12. The method according to any of paragraphs. 1-11, characterized in that the in straight lines (as measured by the size of the image is length), b) the field at the cross-section of the object planes (measured by the size of the image is any of the following: the diameter, area), in) sphere in the section of interest thick slices (measured by the size of the image is the diameter), d) spheroid in section objects thick slices (measured by the size of the image is the axial ratio), d) ellipsoid of rotation when the cross-section of objects with straight lines (as measured by the size of the image is length), e) ellipsoid of rotation when the cross section of the object planes (measured by the size of the image is the axial ratio), W) the ellipsoid of rotation of the projection of objects on a plane (measured by the size of the image is any of the following: area, perimeter), C) a circular cylinder with cross-section of the object planes (measured by the size of the image is any of the following: a small radius large radius, axial ratio, area, perimeter), and round the cylinder when the projection of the objects on the plane (measured by the size of the image is any of the following: area, perimeter), to) elliptic cylinder with cross-section of the object planes (measured by the size of the image is any of the following: small radius, big radius image size is any of the following: square, the perimeter), m) cube when the cross section of the object planes (measured by the size of the image is square), h) cube for the projection of objects on a plane (measured by the size of the image is any of the following: area, perimeter), on the right tetrahedron with the projection of the objects on the plane (measured by the size of the image is any of the following: area, perimeter), p) round cone for the projection of objects on a plane (measured by the size of the image is any of the following: area, perimeter).13. The method according to any of paragraphs. 1-12, characterized in that the images of the objects when the objects interact with stereological probe is obtained using any of the following: a) light microscopy, b) electron microscopy, confocal laser microscopy, x-ray computer tomography, magnetic resonance tomography, ultrasound tomography.14. The method according to any of paragraphs. 1-13, characterized in that the distribution of the size of the images of objects present in any of the following: (a) the curve of the distribution function, b) the curve of the probability density, cumulative frequency histogram, g) histogram of the frequencies d) other graphics functions raspredeleniya, C) the mathematical expectation and variance, to another moment of the distribution, l) another parameter of the distribution function.15. The method according to any of paragraphs. 1-14, characterized in that the approximation when evaluating the degree of matching of the model with the obtained actual size distribution of images of objects provide visual and/or statistical methods (for example, using the criterion^{2}).16. The method according to any of paragraphs. 1-15, characterized in that the size of the objects is any of the following ways: a) specific numerical value, b) set specific numerical values) variable that takes values in the interval of values with a given probability density, g) combinations of the above methods set the size of objects.17. The method according to any of paragraphs. 1-15, characterized in that the relative position of objects and planes section is any of the following ways: a) specific numerical value, b) set specific numerical values) variable that takes values in the interval of values with a given probability density, g) combinations of the above methods of defining the relative position of objects and planes section.18. SPO the bone cross-section, is any of the following ways: a) specific numerical value, b) set specific numerical values) variable that takes values in the interval of values with a given probability density, g) combinations of the above methods of defining the relative orientation of the objects and planes section.19. The method according to any of paragraphs. 1-18, characterized in that the distribution of the size of the images of objects are approximated by the use of modal effect, consisting in equal fashion the size distribution profiles of the cross section of interest to the true value of the size of objects.20. The method according to p. 18, characterized in that the distribution of values of any two angles used to describe the relative orientation of the objects and planes section, set the Gaussian (normal) distribution, or distribution of Dimroth-Watson.

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