Synthetic medium for dilution of semen of birds
The invention relates to agriculture. Synthetic medium for dilution of sperm poultry includes components, g: sucrose 3,0-5,0, glucose 0.1 to 1.5, sodium acetate 0,8-1,2, sodium bicarbonate 0.1 to 0.2, potassium phosphate disubstituted 0.05 to 0.15, oxalic acid 0.01 to 0.05, antibiotic Claforan 50-150 IU 100 cm3, distilled water 100 cm3. In this environment not only destroyed the microbial body in the semen, but still fertilizing ability of sperm during prolonged storage. table 2. The invention relates to agriculture, in particular the artificial insemination of poultry.There are various synthetic environment for sperm dilution birds (A. C. 638334, MKI A 61 D 7/02; A. S. 1291140, MKI A 61 D 7/02).As a prototype of the selected synthetic medium for dilution of semen of birds, including sucrose, sodium acetate, glucose, sodium bicarbonate, potassium phosphate (disubstituted), oxalic acid and distilled water (ed. A. D. Davtyan, A. C. Ivanov, A. A. Semenov. Patent of Russia IPC A 61 D 7/02, 2099025, are recorded. 20.12.97).However, a significant drawback is that it lacks bactericidal drugs. When dlitelnii in the developing sperm microorganisms and accumulation in it of waste products of metabolism.Due to the specifics of the device of the genital organs of male birds in the sperm obtained by the method of massage, significantly more often than in mammals, there is contamination by microorganisms (from 15 to 100 thousand microbial cells in 1 cm3).To destroy the microflora in the semen during the breeding together with the staff of the Moscow Institute for control of veterinary drugs was studied (1986-1989,) and recommended range of products, consisting of penicillin, polymyxin b and kanamycin (CPD). However, in small doses, these antibiotics were not effective, but higher doses were toxic to sperm birds.The present invention is the task of creating a synthetic environment for dilution, long-term storage and transportation of semen of birds, contributing to the destruction of the microflora in the semen, increase security of sperms due to the inclusion of low-antibiotic Claforan.The technical result consists in preserving the activity and survival of sperm, the destruction of the microflora of the sperm and, consequently, increase the fertilizing ability of sperm and chick.Announced Wednesday includes: sucrose 3,0-5,0 g, glucose 0.1 to 1.5 g sodium acetate 0,8-1,2 g, duuu water 100 cm3, characterized in that the composition of the medium additionally includes low-toxic antibiotic Claforan (50-150 ME 100 cm3environment).Example. The sperm get from 10 males, diluted in known environments and in the proposed environment in the ratio of 1:3. Check the impact of the new environment on the activity and survival of sperm and microbial contamination of the sperm. The results are presented in table 1.As can be seen from table 1, the introduction of medium composition for dilution of the sperm poultry antibiotic Claforan significantly increased the survival rate of sperm and killed up to 98% of microbial phoneAfter inspection of the environment on the survival of spermatozoa and the number of microbial cells in the semen after 1 hour storage conducted an assessment of the fertilizing ability of diluted semen. What obtained sperm from 12 males was diluted with a known and a new environment, kept the samples for 24, 48 and 72 hours and seminal chickens for 30 goals in each group. Insemination was performed in the second half of the day after oviposition (15-16 h) dose of 0.1 cm3with an interval of 1 time per 7 days. Oplodotvorennogo eggs was determined on the 7th day of incubation. The results of insemination are presented in table 2.As can be seen from table 2, sanitizing drug CPD (panic is In the new environment of low antibiotic Claforan not only destroyed the microbial body in the semen, but kept the fertilizing ability of sperm during prolonged storage. This is especially important when transporting sperm to the far distance from one household to another.
ClaimsSynthetic medium for dilution of sperm poultry, including sucrose 3,0-5,0 g, glucose 0.1 to 1.5 g sodium acetate 0,8-1,2, sodium bicarbonate 0.1-0.2 g, potassium phosphate disubstituted 0.05 to 0.15 g of oxalic acid 0.01 to 0.05 g, distilled water 100 cm3, wherein the medium further comprises a low-toxic antibiotic Claforan in the amount of 50-150 IU 100 cm3environment.
SUBSTANCE: method involves using product containing spermatozoa treated by drying with freezing to humidity level of 1% and having injured membrane or spermatozoon head so that spermatozoon nucleus retains its genetic validity enough for fertilization. The spermatozoon heads fertilize an isolated oocyte after rehydration and microinjection being done. The retained genetic integrity is enough for fertilizing an oocyte and producing living descendants. Method involves collecting living mature spermatozoa, making spermatozoa suspension in special purpose physiological medium, freezing the spermatozoa suspension for producing frozen spermatozoa, drying the frozen spermatozoa or spermatozoa heads in vacuum to humidity level of 1%, making rehydration of spermatozoa or spermatozoa heads with injured membrane and selecting those retaining nucleus of genetic integrity. The selected spermatozoa or spermatozoa heads are used for fertilizing isolated oocytes with living descendants being produced.
EFFECT: enhanced effectiveness in producing living descendants.
39 cl, 5 dwg, 1 tbl