A method for predicting allergodermatosis the child of early age

 

The invention relates to medicine, in particular to Pediatrics. The method allows to predict allergodermatozy before the appearance of clinical signs with high accuracy, and it's easy to use, non-invasive, retraumatized. In children of early age are conducting a study of the biological fluid, while at the age of 3-6 months in saliva determine the content of slono-spermatozoa globulin (SHBG) and when it is equal to 16 g/ml and above predict the development allergodermatosis. table 1.

The invention relates to medicine, namely to Pediatrics, and can be used to predict allergodermatosis in children of early age in terms of slono-spermatozoa globulin (SHBG) in the saliva.

The urgency of development of this method is determined by a high level and a significant increase of allergic diseases in children (I-III place in the structure of morbidity), among whom young children 57-90% are allergic (2, 5), and a pronounced increase in the number of severe allergic chronic relapsing course, which are difficult to treat (Smirnova, I., 1998).

A known method for predicting the development allergies the koi table including the parents of occupational hazards, pollution of the ambient air, the presence of potential allergens in the home, cases of congenital hereditary diseases in the family, complications of pregnancy and childbirth, diseases of the child in the neonatal period, take into account the age, child's gender, blood group, its housing, feeding (1).

The disadvantages of the method: 1. The use of a large number of historical figures (more than 12).

2. Subjective evaluation anamnestic risk factors.

3. The lack of an objective marker, indicating a high probability of allergic diseases.

4. The lack of data about the accuracy of the method.

The closest technical solution is the method of determining the form allergodermatosis in children of early age by examining the spontaneous adhesion of neutrophils in the peripheral blood of a child with spontaneous adhesion 53,753,48% diagnosed atopic dermatitis, and when the indicator 29,181,83% diagnosed eczema (6).

The disadvantages of the method: 1. Invasiveness.

2. Painful injury of a child.

3. You want a fence at the child's blood.

4. The absence of a Pro who is not all forms allergodermatosis (atopic dermatitis and eczema are found only 24.5-26.3% of cases allergodermatosis in children of early age (5).

6. The lack of data about the accuracy of the method.

These drawbacks are expected to be eliminated in the present method.

The technical solution of the proposed method is forecasting allergodermatosis the child's early age by determining in its saliva at the age of three to six months of detention, slune-spermatozoa globulin (SHBG) and when its level is equal to 16 g/ml and above forecast allergodermatozy accuracy of 86.1%.

SHBG was first identified in 1982, P. O. Shevchenko and co-authors. This is a thermostable alpha-1-alpha-glycoprotein with molecular weight of 60000D. SSG provides women only in the saliva and tissue of the parotid gland, and men in tissues of the testis and sperm. Serum protein does not contain (8).

In earlier studies noted increased levels of SHBG in women with late gestosis (3), as well as in the sperm of men with varicocele and chronic prostatitis (4).

The novelty of the method: the Relationship of the contents of this protein in the saliva of children with development allergodermatosis traced for the first time.

Significant differences: the Establishment in the saliva of children aged three to six months prognostic factor, SHBG (equal to 16 g/ml and above), when coatom.

In three to six months is taken saliva 0.2 ml in glass vial. The analysis is standard in 1% agar gel, where there is a precipitation reaction between the standard test system and saliva samples, serially diluted, respectively- 1:1, 1:2, 1:4, 1:8, 1:16 and so on (7).

Molten agar is poured on fat-free slides. After solidification of the agar on glass, it punched holes special standard stamp "seven". Agar from the wells is removed with a thin needle. In the Central hole of the "sevens" fill in anticigarette against SHBG, and two opposite - standard antigen. In the remaining four investigated saliva at different dilutions. The glass is placed in a Petri dish with a moist environment. The results are read after 18 hours. Considered the last ACC line precipitation near the wells with the appropriate dilution. This breeding is multiplied by the sensitivity of the method - 4 g/ml, i.e., the result is read as 4, 8, 16, 32, etc. g/ml.

The essence of the method is illustrated by the following examples.

Example 1. The child And was born from the first pregnancy, complicated by the threat of miscarriage, mild anemia. Delivery at 40 weeks. Estimation on Apgar scale 8-9. Breastfeeding up to 4 months. At the age of 3 months the child Prognos development allergodermatosis. On the sixth month, the baby developed changes on the skin with pronounced exudative and weeping, lokalizovalsya on the face and on the hands. Diagnosed allergodermatozy, atopic dermatitis. The prediction was confirmed.

Example 2. Baby B was born from the second pregnancy, I genera. History 1 med. the abortion. The mother during pregnancy was noted the threat of miscarriage and mild anemia. Delivery at 39 weeks. Score Apgar 8-9. Breastfeeding up to 4.5 months. At the age of 4 months was symptomatic allergodermatosis, determined the level of SHBG in the saliva, which amounted to 8 mcg/ml was Predicted absence allergodermatosis at an early age. Upon further observation of the child within three years of allergies there was no prediction was confirmed.

Example 3. The child was born from the first pregnancy going on in the background vegetative-vascular dystonia and mild anemia. Delivery at 38 weeks by caesarean section, about a narrow pelvis of the mother. Score Apgar 6-8 points. Breastfeeding up to 3 months. The mother in the history of urticaria, hypersensitivity to the antibiotic penicillin. At the age of 3 months specified level of SHBG in the saliva, it amounted to 64 g/ml gives a forecast on the development of allergodermatit skin, the trunk and extremities, predominantly on the extensor surfaces. The prediction was confirmed.

Advantages of the method: 1. The possibility of forecasting allergodermatosis long before the appearance of clinical signs of disease.

2. Allows timely preventive measures.

3. Distribution forecast at an early age.

4. Non-invasive, retraumatized.

5. Does not require expensive equipment and reagents.

6. The simplicity of the method allows its use in any clinical laboratory.

7. The accuracy of the forecast of 86.1%.

The specified method were examined 70 children. The research results are summarized in the table.

LITERATURE 1. Kazan state medical. University. C. C. Kurashova "a method for predicting the development of allergic diseases in children. Patent 2108582 from 10.09.1998,

2. Clinical immunology and Allergology. Ed., the Lawlor Jr., T. Fisher, D. of Adelman. - M., Practice, 2000. - 806 S.

3. Moiseeva L. C., Panov, I. A. "a Method for the diagnosis of late preeclampsia". Patent 2138817 from 27.09.1999,

4. Sidorov A. P. the Role of male factor infertility and miscarriage in a couple. //Abstract. Diss.... Kida. the honey. Sciences. Ivanovo. - 1997. - 21 S.

5. Smear The Fadeeva O. Y. "a method for the diagnosis form allergodermatosis in children of early age. Patent 2140082 from 20.10.1999,

7. Khramkova N. And., Abelev, And. the Limits of sensitivity of the method precipitation in agar. //Bull. the experts. biology. - 1961. - 12. - S. 25.

8. Shevchenko O. P., Tatarinov, Y. S., Petrunin, D. D. Immunochemical identification and physical-chemical characterization of specific proteins of seminal plasma. //Bull. the experts. biology. - 1982. - So 94. - 10. - S. 80-81.

Claims

A method for predicting allergodermatosis in children of early age by examining the biological fluid, characterized in that in the age of 3-6 months in saliva determine the content of slono-spermatozoa globulin (SHBG) and when it is equal to 16 g/ml and above, predict the development of allergodermatosis.

 

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FIELD: medicine, analytical biochemistry.

SUBSTANCE: invention relates to laboratory methods of investigations. Method involves sampling specimen from patient to be inspected, extraction of serotonin and histamine from a specimen, chromatography of extract and determination of concentration of serotonin and histamine by the fluorescence intensity value. Saliva is used as biological fluid. Saliva by volume 1 ml is extracted with 4 ml of 1 N hydrochloric acid solution, 2 g of anhydrous potassium carbonate and 5 ml of mixture of butanol and chloroform in the ratio 3:2 are added, extract is shaken up and centrifuged. Organic phase (4 ml) is sucked off from extract and passed through chromatography column (diameter is 3 mm, height is 16 mm) filled with ion-exchange resin KB-4 or KB-4P-2 or Bio Rex-70 in H+-form, size of granules is 0.1 0.02 mm. Histamine is eluted with 4 ml of 0.1 N hydrochloric acid at the rate of eluting solution 0.4 ml/min. Histamine concentration is determined by reaction with ortho-phthalic aldehyde dissolved in ethanol. Serotonin concentration is determined by reaction with ninhydrin in organic passed through column. Method provides assaying the saliva concentration of serotonin and histamine with high precision.

EFFECT: improved assay method.

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