A method for predicting the generalization breast cancer

 

The invention relates to medicine, in particular to Oncology. The method provides early preclinical detection generalization of breast cancer. Conducted biochemical research, that you are carrying out dynamic observation of patients with breast cancer, determined in 0.2 ml of blood plasma of patients with platelet activity of monoamine oxidase (MAO-B), with normal platelet activity of MAO-B state a state of remission, and by reducing platelet activity of MAO-B in 4-6 times with respect to the norm predict the generalization process. table 1.

The invention relates to medicine, namely to biochemical research in Oncology, and can be used for planning effective methods of prevention and treatment of generalized forms of breast cancer.

The famous "a Method for predicting cancer of the vulva (see patent 2153175 from 30.06.88, bull. 20), including dynamic observation of patients with vulvar cancer in III clinical group after the cancer treatment; determine the daily excretion of melatonin in the urine, and severe in 2-3 times in comparison with the norm, the increased levels of melatonin in daily urine predict reci is about: a duration analysis, the use of toxic reagents chloroform, concentrated hydrochloric acid, the use of expensive reagents.

Known "Method of predicting recurrence of vulvar cancer (see patent 2023266 from 15.11.94, bull, 21), we have chosen as a prototype, including the determination of prolactin in the serum of patients before treatment, and when the value of its concentration in excess of the 400 ╬ťA/ml, predict recurrence of vulvar cancer as soon as possible.

However, this method has disadvantages, namely: to conduct research requires special equipment, special conditions for radioimmunoassay analysis, expensive, imported test kits RIA; the execution mode of serial studies, accumulation of material required for a long period of time is 10-15 days.

Thus, the methods of forecasting and although fairly efficient, but complex in execution. In addition, these methods of forecasting have been developed and applied for the treatment of patients with cancer of the vulva, making them impossible to use in this aspect of the study of cancer patients at other sites.

The aim of the invention is the early pre-clinical detection of generalization process when R is m, that conduct dynamic monitoring of patients with breast cancer, determine the activity of MAO-B in 0.2 ml of blood plasma of patients with normal activity of MAO-B state a state of remission, and by reducing platelet activity of MAO-B in 4-6 times with respect to the norm predict the generalization process.

The invention of "a Method for predicting the generalization breast cancer" is new, as the set of essential features not known from the level of medicine in the field of biochemical research in Oncology for planning effective treatment methods.

The novelty of the invention lies in the fact that conduct dynamic monitoring of patients with breast cancer, determine the activity of MAO-B in 0.2 ml of blood plasma of patients with normal activity of MAO-B state a state of remission, and by reducing the activity of MAO-B in 4-6 times in relation to normal, predict the generalization process.

Of the analog prototype and it is known that prolactin and melatonin are labile hormonal indicators of the hypophysis and epiphysis and sensitively react on both endogenous and exogenous stimuli. Patients during the examination should not be Podgornoe MAO-B is a stable indicator of monoaminergic system and does not depend on the above factors. According to modern concepts, the Central nervous system (CNS) plays a crucial role in ensuring the homeostasis of the organism. It is established that the failure of homeostatic regulation occurs as a result of lower level neuroamine in the brain and particularly in the hypothalamus. Physiological levels of biogenic amines in the brain are largely supported catalytic activity of monoamine oxidase (MAO). In brain MAO is present in neurons, glia, nerve fibers and the capillary walls. The similarity of metabolism of biogenic amines platelets and neurons in the brain allows the use of platelets as a model for studying the dynamics of MAO in the Central nervous system in a number of diseases. However, such studies in Oncology was conducted. We found that patients with locally advanced breast cancer (Art. III, II CL gr.) there is a decrease in the activity of MAO-B of platelets in 2 times in comparison with the control (healthy women) (table).

The invention of "a Method for predicting the generalization breast cancer" involves an inventive step, as for a specialist therapist oncologist is not obvious from the level of medicine in the field of forecasting rasprostranennye forecasting in malignant diseases, not known in the medical literature and clinical practice, neither in Russia, nor in the CIS or abroad.

The invention is industrially applicable and can be used in health care in Oncology, in various medical institutions of cancer profile, cancer research institutes, oncological dispensaries.

Based on the results of the research on the Method for predicting the generalization breast cancer" we installed: after conducting a comprehensive treatment, including chemotherapy, remote gamma-therapy is recommended (DHT), a radical mastectomy in patients with III clinical group platelet MAO activity was normalized. In patients who are in remission from 1.5 to 5 years, a study was conducted platelet activity of MAO-B levels corresponded to the normal value of 0.670,08. In patients with generalization process (IV CL gr.) observed decrease of enzyme activity more than 4 times (0,140,016) relative to controls and patients III clinical group.

The specific examples of the application of the Method for predicting the generalization breast cancer" Example 1 Patient Thorin L. A., 1940 gelez with metastasis in the left axillary lymph nodes (T2N2M0), stage III, group II. IN V-VIII. 99, a comprehensive treatment (preoperative chemoradiotherapy, VIII.99, radical mastectomy on Pati left, followed DHT on the path of lymphatic drainage). Histological analysis 541513-19 6. VIII.99, - infiltrating ductal cancer. 3.XI 23.XII held on 3 adjuvant chemotherapy according to the scheme CMFAV, hormonal therapy with tamoxifen 20 mg/day long-term. Is under the supervision of a currently diagnosed with cancer of the left breast. Condition after complex treatment (ITRS VIII.99 p T1N0M0), gr. III. Signs of generalized disease is not identified.

If the turnout at the next inspection 12.X.2000 performed a study on the determination of the activity of MAO-B of platelets, which amounted to 0.64 nmol/mg protein/min, which corresponds to normal levels and confirms the remission involves proactive monitoring and do not require treatment.

Example 2 Patient Krokhmal N. In., 1953 R., history 10905, menopause 4 years. From the anamnesis: in 1995-1996, was combined treatment for cancer of the right breast. Histological analysis 5888-95 from 23.1.96, - poorly-differentiated adenocarcinoma. Until the autumn of 2000 was the surveys of the activity of MAO-B, platelet revealed a significant decrease in 6 times (0.1 nmol/mg protein/min). On osteointegrated bones 14.X.2000 showed signs of osteodistraction pubic bone right at the level of 173%. On radiographs of the pelvis from 19. H. 2000 - metastases in the right thigh, in the right pubic bones, the symphysis. Linkincremental examination of other lesions of the visceral organs did not reveal. Started chemotherapy scheme CMAV, hormonotherapy. In the treatment of disease progression to metastatic lesion and root tissue of the right lung. On FLO lung tomograms 14.XI. 2000, performed in the dynamics 17.X.2000, at the root of the right appeared the large size of the node metastases in broncho-pulmonary the lymph node) with the advent of infiltrative changes in S6and in the middle portion of the intermediate wall and the lower lobar bronchus look thickened, infiltrated. The final diagnosis: a Generalization of cancer of the right breast (combined treatment 1995-1999 gg ) with multiple metastases in the bones of the pelvis, the root and the fabric of the right lung, gr. IV. If you continue chemohormonal treatment marked stabilization of the process.

Technical and economic efficiency "Means prognose the Yong; platelet MAO activity-B is defined in a small volume of blood plasma, is performed very quickly (within 3 h) chemicals and equipment are available to provide research, its implementation is possible at any biochemical laboratories. Early diagnosis generalization process to clinical manifestations allows you to plan adequate treatment of the sick, the rational use of medicines.

Claims

A method for predicting generalization of breast cancer, including biochemical, characterized in that conduct dynamic monitoring of patients with breast cancer, determined in 0.2 ml of blood plasma of patients with platelet activity of monoamine oxidase (MAO-B), with normal platelet activity of MAO-B state a state of remission, and by reducing platelet activity of MAO-B in 4-6 times with respect to the norm predict the generalization process.

 

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FIELD: medicine, psychiatry.

SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.

EFFECT: more objective prediction of disease development.

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