The way pathogenetic therapy of chronic liver diseases

 

(57) Abstract:

The invention relates to medicine, in particular to a gastroenterologist, and can be used for pathogenetic treatment of chronic liver diseases. Use a suspension of fetal cells, optionally subjected to at least two irradiation of the laser radiation. A suspension of fetal liver cells and/or spleen and/or thymus injected intraperitoneally. The method increases the content of biologically active substances in fetal tissue. This speeds up the regeneration processes in the liver. 2 C.p. f-crystals.

The invention relates to medicine, in particular to a gastroenterologist, and can be widely used for the treatment of cirrhotic changes and other chronic liver diseases.

There is a method of treatment of chronic diffuse liver diseases involving injection of a suspension of fetal cells in human tissue, and the introduction is carried out subcutaneously in 4-5 points of the anterior abdominal wall, and the course contains 2-4 injections (see Coll. articles edited, So Dry "Transplantation of fetal tissue and human cells". - M., international Institute of biological medicine, 1996, pp. 84-86).

PR is proishodit their transportation, bypassing the liver, lower or upper hollow veins, the right half of the heart, then in the capillary network of the lungs into the left departments of heart and finally systemic blood flow they get to the liver.

In this regard, the number of fetal tissues, reaching the liver is greatly reduced compared with the number of these tissues, the source is introduced subcutaneously to the patient, resulting in their effects on the liver weakens.

Therefore, the above method is not effective enough.

The known method of pathogenetic therapy of cirrhosis of the liver, is selected as the closest analogue, providing injection intraperitoneal injection of a suspension of live fetal hepatocytes (see patent RU 2161036, M. class. And 61 To 35/407, 35/48, publ. 27.12.00).

According to this way of injecting reach the adipose tissue of the round ligament of the liver, where and enter, for example, 8107suspension of live embryonic hepatocytes in 5 ml of physiological solution. To achieve a comprehensive restorative effect and for the stimulation of protective forces of an organism of the patient optionally in the subcutaneous tissue of the anterior abdominal wall, for example the left iliac region, injecting impose a suspension of other fetal is trustwor).

According to the authors of this method, introduced embryonic hepatocytes possess a powerful germ potential, they contain biologically active substances that stimulate cell regeneration of the damaged liver. Coming into contact with cells of a diseased liver, implanted cells stimulate their development and functioning.

Improvement of liver function in objective and subjective data occurs 3-4 weeks after the procedure with subsequent persistent remission of the pathological process.

However, as can be seen, this method is quite complicated, as it involves the introduction of fetal hepatocytes directly into the round ligament of the liver, i.e., requires precision operation. In addition, this method is more time consuming, because it requires the introduction of other fetal cells are additionally not in the subcutaneous tissue.

The known method is also less effective because when the specified number of input suspension improvement over quite a long period of time.

The technical result for the solution of which directed this invention is to simplify the method, the reduction of its duration and higher up the scale the way pathogenetic therapy of chronic liver disease, introducing a recipient of a suspension of fetal cells according to the invention for use of a suspension of fetal cells, optionally subjected to at least two irradiation of the laser radiation. Thus carry out the injection of the suspension of fetal liver cells and/or spleen and/or thymus. And the introduction of a suspension of fetal cells carry intraperitoneally.

Eksperimentalno determined that additional at least two irradiation of a suspension of fetal cells by laser radiation can significantly improve the speed and quality of the regenerative processes in the damaged tissues of the liver by increasing intracellular and cellular regeneration of hepatocytes, increasing number of multi-core and krupnomernyh hepatocytes containing the coarse chromatin, 1-3 nucleoli, and also due to the increasing proliferation of stellate reticulocytes.

The experiments also revealed that it is subjected to laser irradiation fetal cells are able quickly to stimulate the regeneration of damaged liver tissue due to more active actions contained biologically active substances, anti-inflammatory, bacterias the quarterly also detected, the best results are achieved when using a suspension of fetal liver cells and/or spleen and/or thymus.

In addition, the best results are achieved when it intraperitoneal administration declared suspension.

The set of essential features of the claimed object "method" is different from the nearest similar and should not be explicitly studied the prior art that demonstrates its compliance with the criteria of "novelty" and "inventive step".

The invention can be applied in health care that demonstrates its compliance with the criterion of "industrial applicability".

The way pathogenetic therapy of chronic liver diseases such as cirrhosis of the liver, as follows.

The suspension in the experiment was prepared as follows.

Fetal liver tissue taken from a 20-day-old fetuses after slaughter of pregnant female rats of Wistar line.

Suspension of fetal liver cells prepared on the basis of physiological saline in a glass homogenizer with a special play to preserve the integrity of the cells.

Cell breeding 31051 is

Willing suspension of fetal hepatocytes is placed in the tripod in sterile glass test tubes.

The diameter of the fiber is selected so that it fits included in the test tube.

The fiber laser is lowered into the tube and is installed at some distance from the surface of the suspension. The processing is defocused beam. Contact with the surface of the suspension not.

For irradiation was used in the experiment helium-neon laser "Beehive".

The irradiation was performed at least twice for 2 minutes with a break of 5 minutes at a power density of radiation of 75 mW/cm2.

Irradiated suspension injection injected intraperitoneally biological object with a diagnosis of cirrhosis in the amount of 1 ml with dilution 3105. The weight of the biological object 200,

Positive results were observed after 5-7 days.

Similarly prepared and suspensions of fetal cells in the spleen and thymus, and their mixture.

Experiments using the proposed methodology, conducted on animals have shown that the speed and quality of the regenerative processes in tissues of the liver was significantly increased at the expense inside containing the coarse chromatin, 1-3 nucleoli, as well as the increasing proliferation of stellate reticulocytes.

It was confirmed histologically and histochemically.

Example 1. Biological object weighing 200 g of Diagnosis: cirrhosis of the liver stage of remission.

Prepared and irradiated twice for 2 minutes with a break of 5 minutes to a suspension of fetal liver cells breeding 31051 ml 1 ml injected injected intraperitoneally given biological object.

A week later, showed positive results. Animal scored after 3 weeks. Histological and histochemical methods study confirmed the high regenerative capacity of the transplanted fetal cells.

Example 2. Biological object weighing 250 was Diagnosed with cirrhosis of the liver. Remission stage.

The difference from example 1 is used for introducing a suspension of fetal liver cells, spleen and thymus. The suspension was subjected to three times of the irradiation of laser light for 1.5 minutes with an interval of 4 minutes.

Positive results were observed after the fifth day. The results were confirmed histologically and histochemically.

Example 3. Patient E., 38 years. Diagnosis: Chronionych human tissues.

Cooked and processed by the claimed method, the suspension of fetal liver cells and spleen (exposure four times for 1 minute with an interval of 1.5 minutes) cell breeding 3105was administered to the patient intraperitoneally in an amount of 5 ml of suspension.

The patient's health has improved in 1.5 weeks. The results also showed positive shifts. The patient was discharged from hospital after 3 weeks.

1. The way pathogenetic therapy of chronic liver diseases, introducing a recipient of a suspension of fetal cells, characterized in that for use of a suspension of fetal cells, optionally subjected to at least two irradiation of the laser radiation.

2. The method according to p. 1, characterized in that carry out the injection of the suspension of fetal liver cells and/or spleen and/or thymus.

3. The method according to p. 1 or 2, characterized in that the injection of the suspension of fetal cells carry intraperitoneally.

 

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