The method of obtaining the substance of natural honey

 

The invention relates to the pharmaceutical industry. Carry out heating honey to a temperature of more than +40oC. Next, honey defend, clarify, and mixed with bidistilled water during boiling in a mass ratio of 2: 5. The invention allows to obtain the substance, intended for the treatment of chronic hepatitis and cirrhosis of the liver and has immunomodulatory effects. table 1.

The invention relates to the pharmaceutical industry, namely the method of production of the substances isolated from natural honey, for further manufacture on the basis of the substance of the drug for the treatment of chronic hepatitis and liver cirrhosis.

A method of obtaining medicines, which includes freezing to a temperature of -20oWith distilled water, followed by coating the surface of the ice layer was kept at room temperature non-crystalline floral honey in a weight ratio of 4:1 and keeping within 20 days at -20oC. During this period the dissolution occurs honey in ice with subsequent crystallization. Crystallized honey solution incubated at positive tergiverse at a temperature of -14oWith up to ice and subjected to gamma irradiation [1].

The biological activity of the obtained product has a pronounced hepatoprotective effect.

Also known is a method of obtaining an aqueous solution of honey, the essence of which is that the solvent used bidistilled water, which also freeze to get ice at a temperature of not higher than -20oSince, then, on the surface of the ice put a layer of honey in the ratio of 20-25% to the total mass. The resulting mass is kept in the freezer to dissolve the honey in the solvent and the formation of crystals in the whole volume of the solution. Next, the resulting crystals are separated from the solvent and when they get heated solution having bactericidal properties, superior to similar properties of the original product [2].

However, the use of such products and solutions, for example, when cancer will stimulate the growth of both benign and malignant cells of the body due to the presence in the above mentioned drugs, vitamins and enzymes present in honey that trigger the growth of any tissue, including pathological.

Well-known bactericidal anantaraman sugars. As a result of studying slabokontsentrirovannye sugars and appeared preparation of honey consisting of sugars low concentrations, without enzymes and vitamins. Based on identified antiviral effect of the drug, especially its protective effect on metabolic function of the liver was conducted clinical study of the drug in chronic hepatitis and cirrhosis as the least promising for medicamental therapy of diseases.

The task of the invention is to create a substance, which by cultivation saline solution can be obtained the drug from sugars low concentrations without enzymes and vitamins.

The invention consists in the removal of honey vitamins and enzymes, leaving in it the sugar and micronutrients.

This is solved by heating honey to a temperature at which the vitamins and enzymes are destroyed and upon further defending the fall in the insoluble residue (this temperature for honey more than +40oC). Then the honey is clarified (filtered or centrifuged), with sediment leaving the vitamins and enzymes, is a solution of sugars and minerals. This solution was poured into the process of boiling in bidistilled water in proportions 2:5, Phil is nomadology effect.

The final product is a drug for parenteral injection, used in the treatment of chronic hepatitis and cirrhosis of the liver.

The method is as follows.

Natural whole honey in the amount of 255 g is heated in a flask in a microwave oven to a temperature of 80oWith, defend honey at room temperature before the formation of insoluble sludge for about 7 hours. Obtained after settling a lot of honey lighten, such as filter in the suction flask standard filter 10 under vacuum using a vacuum pump, and weigh the remaining amount of honey.

Quantity 250 g of clarified honey poured with stirring in 625 g of boiling bidistilled water (ratio 2:5) receiving 40% solution. After cooling the resulting solution is filtered standard glass filter 3 under vacuum using a vacuum pump.

The resulting solution is substance to receive the above medication, which remained sugar and micronutrients, and vitamins and enzymes are removed as insoluble precipitate when processing honey.

In comparative controlled studies have shown that the resulting substance possesses a strong hepatoprotectant on the effectiveness of the drug "Essentiale", for example, on carbon tetrachloride (tests using CCL4) received substance exceeds "Essentiale" 8 times clinicomorphological indicator that corresponds quantitatively-death criterion. The severity of intoxication toxic hepatitis drug reduces 12 times.

For the study of hepatoprotective activity of matter experiments were conducted at 25 white rats-males (n=25), weighing 250-280 grams.

The test substance was administered to rats intranasal (1 drop in each nostril) at a dilution of 1:50 and 1:100. The course of treatment was 10 days. Standard served as a hepatoprotector "Essentiale" (Yugoslavia), which was used in a dose of 80 mg/kg orally. Duration of treatment was 4 days. Substance and hepatoprotector was applied for two hours prior to the introduction of carbon tetrachloride.

Liver damage in rats caused by carbon tetrachloride (CCL4), administered orally in a dose of 2.5 mg/kg in the form of oil solution for 4 days.

All animals were divided into 4 groups.

For microscopic evaluation of changes in the liver of her pieces were fixed in 10% formalin solution was frozen and stained.

The severity of the morphological changes in the liver of Wistar rats received is a negative change, in the pathological process involved is not more than 1/3 of the slices; (III) the degree of changes in the average weight, in the pathological process involved 2/3 of lobules; IV degree - heavy changes in the pathological process involved clove all over.

The integral assessment of the severity of liver changes in each group of observations was calculated the number of animals with the changes I-II degree (K1), the number of animals with changes of III-IV degree (K2) and found the ratio of these numbers (K1/K2).

The coefficient of the integral evaluation of the morphological changes of rat liver are shown in table.

As can be seen from the table, pathological changes in the liver of the least pronounced (I-II stage) in groups of 3, 4, where we used the test substance, and in animals treated with the test substance in the dilution 1:100, a protective effect was highest.

This substance at a dilution of 1:100 has superior efficacy of the drug "Essentiale" 8 times clinicomorphological indicator that corresponds quantitatively-death criterion. The severity of intoxication toxic hepatitis drug reduces 12 times.

Sources of information 1. RF patent 2155051 C1 from 01.11.99, publ. 27.08.2000.

2. RF patent 2017435 C1 from 23.08.91, publ.La get hepatoprotector, including heating honey to a temperature above the 40oWith upholding it to obtain an insoluble precipitate, lightening and blending with bidistilled water during boiling in a mass ratio of 2:5.

 

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FIELD: pharmaceutical and food industry.

SUBSTANCE: invention proposes the composition that comprises the following components, wt.-%: bee breed homogenate, 98.5; sorbic acid, 0.75, and citric acid, 0.75. The proposed composition of bee breed preserved homogenate is more stable in storage.

EFFECT: improved properties of composition.

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