Prophylaxis and treatment of degenerative changes of joints and method thereof

 

(57) Abstract:

The invention relates to medicine, namely to pharmacy, in particular to the drugs used for the prevention and treatment of osteoarthritis and other lesions of the joints, accompanied by degenerative changes (DDI) articulatory and paraartikulyarnye tissues. The invention lies in the fact that the proposed composition comprises uronic acid, hexosamine, calcium, phosphorus, magnesium, amino nitrogen in the form of free amino acids and oligopeptides. In obtaining pharmaceutical compositions used for bone mineral and hydrolyzed cartilage, which after discharge are mixed and dried. To obtain the ingredients of the pharmaceutical compositions used acid hydrolysis of cartilage and recalcination bone. The invention provides the possibility of making compositions in clinical and laboratory conditions according to individual indications. 2 S. p. f-crystals.

The invention relates to medicine, namely to pharmacy, in particular to the drugs used for the prevention and treatment of osteoarthritis and other lesions of the joints, accompanied by degenerative changes (DDI) articulary and a couple of the disruption of the normal processes of degradation and synthesis of chondrocytes, extracellular matrix and subchondral bone. The last involvement in the pathological process is often accompanied by symptoms of osteoporosis [1, 2].

Currently known food additive "Inoltra", which is a synthetic tool that combines components of articular cartilage glucosamine and chondroitin sulfate, analgesic and anti-inflammatory ingredients - essential polyunsaturated acids and antioxidants, and is used for treatment of degenerative and inflammatory changes in the joints [3].

However, the use of expensive drug "Inoltra" is not always justified, since di joints may not be accompanied by signs of inflammation and disruption of the balance of interactions oxidation reactions and antioxidative. In such cases, the use of antioxidants and anti-inflammatory components is not advisable. In particular, this applies to deforming osteoarthritis associated with age-related changes in the joints. In addition, the mineral component of such drugs should be easily assimilated in the body form.

A method of obtaining vysokochastotnogo and easily digestible calcium - Stimson-4" based on the si precipitate with acid hydrolysate of collagen [4].

However, the known pharmacological composition "Stimson - 4" has in its composition of hydrolyzed glycosaminoglycans in the articular cartilage, and the method of its production is too complicated.

The objective of the invention is to develop a pharmacological composition and its preparation, which includes hydrolyzed glycosaminoglycans, providing for the manufacture of a composition in the clinical laboratory environment according to individual indications.

This task is solved in that in a method of producing a pharmaceutical composition for the prevention and treatment of degenerative changes of the joints, including declinatio bone matrix, acid hydrolysis, the components are mixed and dried, hydrolyzed glycosaminoglycans in the articular cartilage obtained from cartilage purified trachea bovine (cattle), for which they are subjected to acid hydrolysis in hydrochloric acid to dissolve, then decalcomania solution of hydrochloric acid and precipitation of calcium oxide from peeled and crushed bones of cattle receive bone mineral followed by mixing mineral phases with hydrolyzed cartilage and h is VNO-degenerative changes of the joints, including calcium, phosphorus and mixtures of amino acids, which comprises uronic acid and hexosamine, when this composition per 100 g of product is: uronic acid is 2.8-3.2 g; hexosamine 4,8-5,2 g; calcium 42,0-45,0 g; phosphorus of 27.0-30.0 g; magnesium 2,6-2,8 g; else amine nitrogen in the form of free amino acids and oligopeptides.

When the prophylaxis and treatment of di joints sore for 3-4 weeks oral takes calculated in accordance with body weight daily dose of the drug.

The invention being patented explain detailed description, specific examples of execution.

The method of obtaining the pharmaceutical composition is as follows.

Must be cleaned of soft tissue and powdered cartilage of the trachea cattle (cattle) is subjected to acid hydrolysis under vacuum under reflux until complete dissolution of cartilage to obtain uronic acids and hexoses.

Bones of cattle, cleaned of soft tissue and crushed, pour a solution of hydrochloric acid, incubated at room temperature. Bone matrix is separated from the supernatant on a Buechner funnel. To the hydrolysate dobavlyaut distilled water, wring out and dry. The obtained components are mixed. The proposed method allows to obtain a pharmaceutical composition with the following composition composition per 100 g of product, such as uronic acid, 2,8-3,2 g; hexosamine 4,8-5,2 g; calcium 42,0-45,0 g; phosphorus of 27.0-30.0 g; magnesium 2,6-2,8 g; else amine nitrogen in the form of free amino acids and oligopeptides.

The exemplary embodiment of the pharmaceutical composition and method of reception.

Trachea cattle cleaned of soft tissue, is crushed to pieces by 5x5 mm

120 g of crushed cartilage tissue is placed in a round bottom flask, equipped with reflux condenser, flask pour 200 ml of 0.8 M HCl solution. The reaction mass is heated on a sand bath until t=102oC and hold under vacuum acid hydrolysis of the cartilage tissue within 24 h until complete dissolution of cartilage to obtain uronic acids and hexoses.

400 g peeled from soft tissues, washed using detergent, repeatedly rinsed with distilled water, crushed to the size of 5x5 mm bones of cattle placed in a container made of chemically resistant material, pour 2 liters of 2N Hcl solution, incubated at room temperature in the Bone matrix is separated from the supernatant on a Buechner funnel. To the hydrolysate added 160 g of calcium oxide, the pH of the reaction mass when this is set ravnim to 8.0, and the resulting solution drop crystals cream color. Separate them with a Buechner funnel, washed repeatedly with distilled water, squeezed and dried at t= 60oC.

The resulting hydrolyzed cartilage mixed with mineral isolated from bone tissue of cattle. The result is 120 g of the composition, which is Packed to 0.5 g in hard gelatin capsules. In 100 g of finished product contains: uronic acids of 2.8-3.2 g; hexosamine 4,8-5,2 g; calcium - 42,0-45,0 g; phosphorus of 27.0-30.0 g; magnesium 2,6-2,8 g; else amine nitrogen in the form of free amino acids and oligopeptides.

The proposed method of producing a pharmaceutical composition technologically simple, low cost raw materials, reagents and equipment, does not require significant financial investments and allows it to be used in the laboratory of the medical institution according to individual indications for patients with diseases of the joints.

The proposed pharmaceutical composition allows to compensate the basic components of articular and paraartikulyarnye tissues suffering from joint, Moi

1. Boonen , S., Aerssens J., Mbuyi-Muamba J. M., Dequeker J. Generalized osteoarthritis protecting against osteoporetic fractures: review of the evidence. J French Brasserie Rheumatol. 1996; 9: 69-73.

2. Arden, N. K., Nevitt, M. C., Lane, N. E. et al. Osteoarthritis and risk of falls, rates of bone loss, and osteoporotic fractures. Arthritis Rheum. 1999; 42: 1378-85.

3. U.S. patent 5840715; application Number: 952272; filing date: 6.01.98; Assignee: Inoltra.

4. Application 95117043, Russia. IPC7AND 61 IN 17/56. The drug Stimson-4" for the correction of metabolism in the body of calcium phosphate and method of its production/ Desatnick K. S. (RF), Luneva S. N.(RF); RISC "RTO" them. Acad. G. A. Ilizarov (RF) - application 95117043/14 (029705); claimed 05.10.95; publ. 10.02.98.

1. Pharmaceutical composition for the prevention and treatment of degenerative changes of the joints, including calcium, phosphorus and mixtures of amino acids, characterized in that its composition it contains uronic acid and hexosamine, when this composition per 100 g of product is, g:

Uronic acid - 2,8-3,2

Hexosamine - 4,8-5,2

Calcium - 42,0-45,0

Phosphorus - 27,0-30,0

Magnesium - 2,6-2,8

the rest of amine nitrogen in the form of free amino acids and oligopeptides.

2. A method of obtaining a pharmaceutical composition for the prevention and treatment of degenerative-dystrophic changes sustainabilityis fact, what cartilage purified trachea cattle (cattle) is subjected to acid hydrolysis in hydrochloric acid to dissolve, thus receive hydrolyzed glycosaminoglycans in the articular cartilage, and then spend declinatio solution of hydrochloric acid and precipitation of calcium oxide peeled and crushed bones of cattle, you get a bone mineral and mixed mineral phase with hydrolyzed cartilage.

 

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