The way to increase solsticetm rice

 

(57) Abstract:

The invention is intended for use in agriculture to improve solsticetm of Fig. The method includes pre-sowing seed treatment with a solution of saleproscar based on the double-amide copolymer of acrylic acid and triacilglycerides-1,3,5-triazine (SPAA) in the following ratio of ingredients, wt. %: SPA 0,0001-0,005, water - the rest. The method allows to increase the productivity of plants in conditions of chloride salinity. table 2.

The invention relates to the field of agriculture, particularly to a method of increasing solsticetm rice.

Known heterochain double amide copolymer of acrylic acid and triacilglycerides-1,3,5-triazine (SPA) as a foaming agent for pre-treatment of corn seed (see N.In. Stukalova, C. N. Sapony, G. M. Poghosyan. Seed treatment of maize with the foaming agent-based hetero-chain water-soluble copolymer. - Proceedings Of The Cube.Kai, 1990, vol. 307(335), 10-13). The use of this copolymer as saleproscar to increase solsticetm of rice plants is not known.

Also known is a method of obtaining salt-tolerant forms of sunflower with premeditation N-nitrosodiethylamine concentration 0,008-0.12% 20-25oC for 6-12 h (see Y. D. Beletsky, E. K. Razorflame, L. I. Sizova. The method of obtaining salestracking forms of sunflower. - A. S. 1576063 (USSR); M. class. 5 a 01 N 1/06, bull. Fig. 1988, 25 - prototype). The disadvantages of this method is the need for lengthy pre-soaking seeds, used for treatment of seeds toxic, mutagenic and carcinogenic N-nitrosodiethylamine, as well as a very weak increase solsticetm other crops, such as rice, and the impossibility of increasing the activity of photosynthesis, the length and weight of roots and seedlings, panicle weight and rice yield when growing it in saline sodium chloride soil (according to the authors).

Technical solution to the problem of increasing the length of sprouts, length and weight of roots, photosynthesis productivity, weight of grain in a panicle, weight of 1000 grains and rice yield when grown on the background chloride salinization is the application tool that eliminates the need for lengthy pre-soaking of seeds in water, non-toxic, does not possess mutagenic and carcinogenic effect, with more active calprotectin effect and is able to increase the activity of photosynthesis.

The task of dostigao the si grains in the panicle, mass of 1000 grains, grain productivity, and to increase the activity of photosynthesis in growing rice plants in the background chloride salinity using a method based on pre-treatment of rice seeds by the tool based on the double-amide copolymer of acrylic acid and triacilglycerides-1,3,5-triazine (SPAA) at a concentration of 0.0001-0.005 wt.% when the norm at the pre-processing 10 l/tonne of seed.

The novelty of the claimed proposal is seen that the use of SPA allows you to extend the length of sprouts, length and weight of roots, a lot of grains in the panicle, 1000-grain weight and grain productivity, and increase the photosynthesis of rice plants when growing them in the background chloride salinization; exclude by prolonged pre-soaking of seeds in water, non-toxic, mutagenic and carcinogenic effect and stronger calprotectin action.

At the same time, SPAA receive and purify the chain-radical copolymerization in solution, in water amide of acrylic acid, triacilglycerides-1,3,5-triazine at a concentration below the threshold are stapled by a known method (see N.In. Stukalova, C. N. Zaplishny, G. M. Poghosyan. About the B. Kai, 1990, vol. 307(335), S. 10-13). For comparison, using the sales of the drug N-nitrozometilmochevinu brand "chemically pure" in known concentration of 0.12 wt.%. On the basis of SPAA and N-nitrosodiethylamine prepare aqueous solutions of funds for presowing treatment of seeds. In the case of the known method and the preparation of N-nitrosodiethylamine (prototype) subjects in seeds subjected to preliminary swelling in water for 16 h, whereas in the case of the proposed method and SPA this operation is excluded.

As object of research chose grown in flooded conditions grain rice varieties "Spalicek". For control used seeds, soaked in water. Specific application examples are presented below.

Example 1. Prepared for the experience of rice seeds in quantity 240 pieces are placed in a Petri dish, pour 62,8 ál (10 l/t seed) of water, mix thoroughly until complete wetting and dried at 20-25oC for 0.5 hours Then for laboratory experiences seeds are sown in filled with soil standard sizes germinators (60 seeds/restyling in triplicate) and grown according to the standard technique. For the growing experience of the seeds are sown in filled with soil standard razmerov> Example 2. Preparation and cultivation of rice seeds is carried out in conditions similar to those in example 1, with the only difference that the seeds sown in the soil, with a specially-made sodium chloride in the amount of 0.2 wt.% (2 g 998 g of soil).

Example 3. Prepared for the experience of rice seeds in quantity 240 pieces are placed in a Petri dish, pour 62,8 ál (10 l/t seed) aqueous solution of SPAA concentration of 0.01 wt.%, mix thoroughly until complete wetting and dried at 20-25oC for 0.5 hours Then the seeds are sown and grown in saline (0.2 wt.% sodium chloride) soil under conditions analogous to example 2.

Other examples (examples 4-9) and the results of the experiments are presented in table. 1-2. In examples 4-9 seeds of rice grown in saline (0.2 wt.% NaCl) soil, and in example 9 seeds subjected to preliminary swelling in water (16 h at 20oC) and pre-treatment with an aqueous solution of N-nitrosodiethylamine (to 0.12 wt.%).

The length of sprouts, length and weight of roots of rice plants is determined on the 10th day of the results of laboratory experience according to the standard technique.

The value of the second maximum (m) of the induction curve of the delayed fluorescence (IR PD) as an indicator of activity prostoy method Tretyakov (see G. I. Tretyakov. Physiological studies of salinity tolerance of rice: Diss. Kida Biol Sciences. Tbilisi, 1983, 186 S.).

The structure parameters of the yield (weight of grain in a panicle, 1000-grain weight and yield) is determined by achieving full ripeness in pot experiment.

Obtained by counting and averaging the three replicates in each example, the data mathematically accurate and summarized in table.1 and 2.

As can be seen from the data table. 1 and 2, the application of the proposed method improve solsticetm rice (examples 1-9) provides in comparison with control marked increase in the resistance of rice to salinity. Thus, from the comparison of examples 1 and 2 shows that in the absence of pre-sowing seed treatment of SPAA, rice cultivation in the conditions of chloride salinity leads to a sharp (17-30% in comparison with control) decline all of the vital indicators of the development of the vegetation and productivity. Pre-sowing treatment of seeds of rice on the proposed method with an aqueous solution of SPAA in optimal (of 0.0001 to 0.05 wt. %) the concentration of provides in comparison with control, increasing the length of shoots and roots of 3.5 to 10.5 and 48.1 - 62,2%, respectively (128,5-137,2 and 102,8-112,6 instead 124,2 and 69.4 mm, respectively), while processing is the mass of sprouts and roots during seed processing, SPAA in comparison with control occurred 24.4-53,1 and 15.9 38.2 percent, respectively (40,3-49,6 and 40,1-to 47.8 mg instead 32,4 of 34.6 mg, respectively), while processing the N-nitrosomethylamino increases these figures 4.3-4.6 per cent respectively.

The value of the second maximum (m) IR PD in examples 4-7 (seed treatment, SPA in optimalnoe concentration) 11.6-36,4% superior to that in control (27,0-33,0 instead 24,2 Rel.ed, respectively), while the processing of N-nitrosomethylurea (example 9) did not increase this index

The weight of grain in the same panicle, 1000-grain weight and grain yield in the proposed method, seed treatment of rice SPA at optimal concentration (examples 4-7) increases in comparison with control 12.3-50,8; 0.8 to 3.5 and a 3.5-22.3%, respectively (0,73-0,98; 27,4-28,15 and of 6.49-to 7.67 g instead of 0.65; 27,19 and 6,27 g, respectively) while seed treatment of N-nitrosomethylurea only increases the mass of the grains in the panicle 3.07% in comparison with control, and the rest of the data are even negative in comparison with control values.

Thus, the application of the proposed method improve solsticetm rice seed pre-sowing treatment of their aqueous solutions, SPA can increase the length and weight of sprouts and roots, to intensify the process of photosynthesis and increase the productivity of rice plants when growing them in ispolzovanie for presowing seed treatment with mutagenic and carcinogenic N-nitrosodiethylamine.

The way to increase solsticetm rice, including pre-sowing seed treatment with calprotectin, characterized in that as saleproscar use the tool based on the double-amide copolymer of acrylic acid and triacilglycerides-1,3,5-triazide (SPAA) in the following ratio of ingredients, wt.%:

SPA IS 0.0001 - 0,005

Water - the Rest

and the seeds are treated using dry etching rate of 10 l/tonne of seed.

 

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