The way to increase plant tolerance of rice to salinity

 

(57) Abstract:

The invention is intended for use in agriculture. The method includes pre-sowing seed treatment with a solution of saleproscar based ternary copolymer of acrylic acid, its amide, triacilglycerides-1,3,5-triazine (SPACH) in the following ratio of ingredients, wt. %: SPACH 0,0001-0,005, water the rest, while the seeds are treated using dry etching rate of 10 l/T. the Method allows to increase the yield on the background chloride salinity. table 2.

The invention relates to the field of agriculture, particularly to a method of improving plant tolerance of rice to salinity.

Known heterochain ternary copolymer of acrylic acid, its amide, triacilglycerides-1,3,5-triazine (SPACH) as an antistatic agent (see L. C. Novitskaya, V. N. Zaplishny, L. N. Skripnichenko, ,, Latysheva, H. I. The Sosinovich, G. M. Poghosyan, V. A. Soldatov. An antistatic composition comprising hexahydrotriazine copolymer. - Russian Academy of Sciences, 1989, 5, S. 115-117). The use of this copolymer as saleproscar to increase the resistance of rice plants to salinity is unknown.

Also known spooka in water for 16 to 20 h, followed by treatment of seeds with a solution of N-nitrosodiethylamine concentration 0,008-0.12% 20-25oC for 6-12 h (see Y. D. Beletsky, E. K. Razorflame, L. I. Sizova. The method of obtaining salestracking forms of sunflower. A. C. 1576063 (USSR); M. class. 5 a 01 N 1/06. Bull. Fig. 1988, 25 - prototype).

The disadvantages of this method is the need for lengthy pre-soaking seeds, used for seed treatment of toxic, mutagenic and carcinogenic N-nitrosodiethylamine, as well as a very weak increase solsticetm other crops, such as rice, and the impossibility of increasing the activity of photosynthesis, the length and weight of roots and seedlings, panicle weight and rice yield when growing it in saline sodium chloride soil (according to the authors).

Technical solution to the problem of increasing the length of sprouts, length and weight of roots, photosynthesis productivity, the mass of the grains in the panicle and yield of rice grown on the background of chloride salinity is the application tool that eliminates the need for lengthy pre-soaking of seeds in water, non-toxic, does not possess mutagenic and carcinogenic effect, with more active calprotectin effect and is able to increase the activity of photosynthesis.

The task is achieved tassa roots, weight of grain in a panicle, grain productivity, and to increase the activity of photosynthesis in growing rice plants in the background chloride salinity using a method based on pre-treatment of rice seeds means on the basis of a ternary copolymer of acrylic acid, its amide, triacilglycerides-1,3,5-triazine (SPACH)in a concentration of 0.0001-0.005 wt.% when the norm at the pre-processing 10 l/tonne of seed.

The novelty of the claimed proposal is seen that the use of SPACH can increase the length of sprouts, length and weight of roots, a lot of grains in the panicle and grain productivity, and increase the photosynthesis of rice plants when growing them in the background chloride salinization; exclude by prolonged pre-soaking of seeds in water, has no toxicity, mutagenic and carcinogenic effect and stronger calprotectin action.

This SPACH and get clear chain-radical copolymerization in solution, in water, acrylic acid, its amide, triacilglycerides-1,3,5-triazine at a concentration below the threshold are stapled by a known method (see L. C. Novitskaya, V. N. Zaplishny, L. N. Skripnichenko, gg L is aderrasi copolymer. - Russian Academy of Sciences, 1989, 5, S. 115-117). For comparison, using the sales of the drug N-nitrozometilmochevinu brand "chemically pure" in known concentration of 0.12 wt.%. Based on the SPACH and N-nitrosodiethylamine prepare aqueous solutions of funds for presowing treatment of seeds. In the case of the known method and the preparation of N-nitrosodiethylamine (prototype) subjects in seeds subjected to preliminary swelling in water for 16 h, whereas in the case of the proposed method and SPACH - this operation is excluded.

As object of research chose grown in flooded conditions grain rice varieties "Spalicek". For control used seeds, soaked in water. Specific application examples are presented below.

Example 1. Prepared for the experience of rice seeds in quantity 240 pieces are placed in a Petri dish, pour 62,8 of microlitres (10 l/t seed) of water, mix thoroughly until complete wetting and dried at 20-25oC for 0.5 hours Then, for laboratory experiences seeds are sown in filled with soil standard sizes germinators (60 seeds/restyling in triplicate) and grown according to the standard technique. For the growing experience of the seeds are sown in filled with soil standard is the IR.

Example 2. Preparation and cultivation of rice seeds is carried out in conditions similar to those in example 1 with the only difference that the seeds sown in the soil, with a specially-made sodium chloride in the amount of 0.2 wt.% (2 g 998 g of soil).

Example 3. Prepared for the experience of rice seeds in quantity 240 pieces are placed in a Petri dish, pour 62,8 of microlitres (10 l/t seed) aqueous solution SPACH concentration of 0.01 wt.%, mix thoroughly until complete wetting and dried at 20-25oC for 0.5 hours Then the seeds are sown and grown in saline (0.2 wt.% sodium chloride) soil under conditions analogous to example 2.

Other examples (4-9) and the results of the experiments are presented in table. 1-2. In examples 4-9 seeds of rice grown in saline (0.2 wt.% NaCl) soil, and in example 9 seeds subjected to preliminary swelling in water (16 h at 20oC) and pre-treatment with an aqueous solution of N-nitrosodiethylamine (to 0.12 wt.%).

The length of sprouts, length and weight of roots of rice plants is determined on the 10th day of the results of laboratory experience according to the standard technique.

The value of the second maximum (m) of the induction curve of the delayed fluorescence (IR PD), as an indicator of the activity of the well-known technique Tretyakov (see G. I. Tretyakov. Physiological studies of salinity tolerance of rice. Diss. Kida. Biol. Sciences. Tbilisi, 1983.-186 C.).

The structure parameters of the yield (weight of grain in a panicle, 1000-grain weight and yield) determined to achieve full ripeness in pot experiment.

Obtained by counting and averaging the three replicates in each example, the data mathematically accurate and summarized in table. 1 and 2.

As can be seen from the data table. 1 and 2, the application of the proposed method improve solsticetm rice (examples 1-9) provides in comparison with control marked increase in the resistance of rice to salinity. Thus, from the comparison of examples 1 and 2 shows that in the absence of pre-sowing treatment of seeds SPACH, rice cultivation in the conditions of chloride salinity leads to a sharp (17-30% in comparison with control) decline all of the vital indicators of the development of the vegetation and productivity. Pre-sowing treatment of seeds of rice on the proposed method aqueous solution SPACH in optimal (of 0.0001-0.005 wt. %) the concentration of provides in comparison with control, increasing the length of shoots and roots 8.6-13,9 and 36.2-60,8%, respectively (134,9-141,5 mm 94.5-111,6 mm instead 124,2 and 69.4 mm, respectively) at that time, as obrinje mass of seedlings and roots seed treatment SPACH in comparison with control occurred 31.5-54,6 and 13.3-42,2%, respectively (42,6-50,1 and 39.2 and 49.2 mg instead of 34.2 and 34.6 mg, respectively) at the time as the processing of N-nitrosomethylurea increases these figures only 7.7 and 5.5%, respectively.

The value of the second maximum (m2IR PD in examples 4-7 (seed treatment SPACH in optimal concentration) of 2.5% and 6.6% superior to that of the control (24,8-25,8 Rel. units instead 24,2 Rel.ed. respectively) at the same time as the processing of N-nitrosomethylurea in General does not increase this figure (example 9).

The weight of grain in one panicle and grain yield in the proposed method with pre-treatment grain rice SPACH at optimal concentration (examples 4-7) increases in comparison with the control 26-63,1, and 45.6 to 58.2%, respectively (0,82-1.06 and 9,13-9,92 g instead of 0.65 and 6,27 g, respectively) at the same time as seed treatment of N-nitrosomethylurea only increases the mass of the grains in the panicle by 7.7% in comparison with the control, and the index yields even has a negative value in comparison with the control.

Thus, the application of the proposed method of increasing the resistance of rice plants to salinity pre-sowing seed treatment of rice solutions SPACH can increase the length and weight of sprouts and roots, to intensify the processes of photosynthesis and increase the productivity of rice plants at wires and use for pre-treatment mutagenic and carcinogenic N-nitrosodiethylamine.

The way to increase plant tolerance of rice to salinity, including pre-sowing seed treatment, characterized in that as saleproscar use the tool on the basis of a ternary copolymer of acrylic acid, its amide, triacilglycerides-1,3,5-triazine (SPACH) in the following ratio of ingredients, wt. %:

SPACH IS 0.0001-0,005

Water - the Rest

and the seeds are treated using dry etching rate of 10 l/tonne of seed.

 

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