The method of obtaining iron-containing drug for the treatment and prevention of iron deficiency anemia in animals

 

(57) Abstract:

The invention relates to the field of veterinary medicine. The method of obtaining iron drug lies in the interaction of organic acids with gemostimuliruyuschee properties and composition of iron with the formation of water soluble complexes with organic acids with gemostimuliruyuschee properties use humic and / or fulvic acids isolated from peat extracts, as the iron compounds used water-soluble salts of divalent iron, which take in the amount equivalent to the content of carboxyl groups of humic and / or fulvic acids. Depending on the conditions of processing extracts of peat can get a variety of water-soluble iron-containing preparations. Complex Ferragamo produced by interaction of humic acids isolated from an extract of peat by treating it with a solution of hydrochloric acid at pH 1-2 with sea salt. Complex - ferrofluid obtained from the extract of peat after separation and removal of humic acid by interaction with salt Mora. Complex - verropoulou receive a direct effect on the extract of peat salt Mora. The method allows to obtain products with bale and safe environment. 3 C.p. f-crystals, 2 tab.

The invention relates to the field of drugs and their receipt for the treatment and prevention of iron deficiency anemia in animals.

A method of obtaining water iron drug /1/ in the interaction of compounds of iron and soluble polymers that can form complexes with iron ( III ) when heated, and the polymer used compounds obtained by the interaction in aqueous solution of a hydroxycarboxylic acid, a polyhydric alcohol and epoxidised component with subsequent processing of the reaction products by cyanide and hydrolysis of the resulting cyanhydrin.

The disadvantage of this method is that to obtain the drug use of soluble polymers that do not have hemodialysis properties, which leads to low efficiency of the final product. In addition, the source reagents-road complexing agents and their use greatly increases the cost of the target product. The use of cyanide and epoxidised component increases the risk that the target product is toxic.

A method of obtaining diglutamate iron (II) /2/ taken for the prototype and zakluchalsya metallic iron and the process is conducted in the presence of copper oxide (I) or (II) when the molecular ratio: glutinosa acid:iron:copper oxide 2:1.5 to 2:1.

The disadvantage of this method is the complexity of the process requires expensive equipment and strict observance of the rules of safety when working with hazardous substances (hydrogen).

The inventive method of obtaining iron drug aims to eliminate the above disadvantages and is in the interaction of organic acids with hemodialysis properties and composition of iron with the formation of soluble complexes, and the new is that as organic acids with gemostimuliruuchee properties apply humic and / or fulvic extracts of peat, as well as compounds of iron, for example, salt Mora, which is taken in an amount equivalent to the content of carboxyl groups of humic and / or fulvic acids.

Depending on the conditions of processing extracts of peat can get a variety of water-soluble iron-containing preparations with hemodialysis properties.

Complex Ferragamo produced by interaction of humic acids isolated from an extract of peat by treating it with a solution of hydrochloric acid at pH 1-2 and the compound iron (II), for example salt Mora. Obrazovanja peat after discharge from naked humic acids and the process of complexation interaction fulvic acids with a compound of iron (II), for example salt Mora. Formed a stable complex is dried.

Complex verropoulou obtained from the extract of peat containing humic and fulvic acids, the interaction of the extract of peat and compound iron (II), for example salt Mora. Formed a stable complex is dried.

The claimed invention differs from the prototype in that as organic acids with gemostimuliruyuschee properties apply humic and / or fulvic acids isolated from extracts of environmentally friendly natural product of peat. Humic and fulvic acids compared with glutamic acid used in the method prototype, have a higher gemostimuliruuchee activity.

Another difference is that as the iron compounds in the process of complexation apply water-soluble salt of Mora, which allows to obtain the final product, containing in its composition digestible iron (II). The product obtained by the method of the prototype, contains iron of different valency, which reduces iron absorption in the intestine. In addition, in order to convert the metallic iron in an ionized form, requires expensive about the conditions of complex formation eliminates the possibility of oxidation of divalent iron to trivalent trudnousvoyaemoe form. The use of iron (II) salt composition Mora facilitates obtaining water-soluble complexes. It should be noted that as compounds of iron (II) can be used not only salt Mora, but other water-soluble salts of divalent iron.

The advantage of the proposed method is that of the specified source reagents can be of various drugs with the specified range of therapeutic activity.

So, ferragut that you want to select from extracts of peat at pH 1-2 with a view to full highlight, actively stimulates the recovery of hemoglobin in the blood with iron deficiency anemia.

Ferrofluid, isolated from an extract of peat after removal of humic acids, actively stimulates cell division predecessors erythropoiesis.

Verropoulou receive a direct effect on the extract of peat ferrous iron salt, such as salt Mora. Verropoulou effectively (better than others) increases reticulocytes the reaction of the brain.

In comparison with the known official drug ferrochromium /3/ made in Yugoslavia and recommended for treatment and prevention of iron deficiency anemia, one tablet catapano the release of iron in the small intestine, the products obtained in the described manner, also have advantages, because they have gemostimuliruyuschee properties. Yugoslav drug is inferior to the preparations obtained by the claimed method, as they are not fully eliminates the toxicity inherent in inorganic iron salts (table.2).

Example I. Receiving ferrohogbomite.

Salt Mora in the number 29,008 g (0.15 g-EQ Fe2+) dissolved in 200 ml of distilled water. In the resulting solution with constant stirring to 1000 ml of the extract of peat containing 4,2 g (0.15 g-EQ carboxyl groups) of dry organic matter. The resulting mixture was kept under stirring for 1 hour. Two days after the product is dried at a temperature of 20-25oC.

Example 2. Getting Ferragosto.

To 500 ml of the extract of peat containing 0.03 g-EQ carboxyl humic acid, poured a dilute solution of hydrochloric acid to pH 2, twice washed precipitated humic acid decontarea. Selected humic acid adjusted to pH 7.5 with ammonia, then gradually add 5,706 g (0.03 g-EQ Fe2+salt Mora, dissolved in 50 ml of distilled water, and incubated with constant stirring for 1 hour. Che is ramasami centrifugation. The product is dried at a temperature of 20-25oC.

Example 3. Getting perfoliata.

Salt Mora in the number 8,798 g (0,045 g-EQ Fe2+) dissolved in 50 ml of distilled water, poured into the filtrate obtained in example 2 and containing 0,045 g-EQ carboxyl groups of fulvic acids. The mixture was kept under stirring for 1 hour and dried at a temperature of 20-25oC.

Chemical composition and solubility in water all the preparations are presented in table.1. The effect of drugs on blood parameters (the value of the integral indicator in % from baseline PF) are presented in table.2.

Biological tests obtained herocomplex for the treatment of iron deficiency anemia were carried out on 60 outbred white mice-females. 50 mice was simulated iron deficiency anemia (single acute loss of 30% of circulating blood);10 animals served as background. An hour later after blood loss and then daily 1 time per day to mice 3 experimental groups were injected intragastrically through the probe herocomplex in 1% starch mucus. The positive control group were asked by an appropriate number of officinal drug ferrochromite in 1% starch mucus. The control group, p is considered only contained in a preparation of soluble iron.

The value of the integral index (PI) was calculated by the formula /4/:

< / BR>
where n is the number of terms measurement;

ij is the value of the j-th index in the i-th term of measurement;

j(o) is the initial value.

PI characterizes the dynamics of peripheral blood anemizirovannam mice (control) and received one of the iron-containing preparations (table.2).

The results of our experiments indicate that the drugs obtained by the proposed method is effective for the treatment of iron deficiency anemia and have a specified range of therapeutic activity; thus, Ferragamo more than other drugs stimulates the recovery of hemoglobin in the blood with iron-deficiency anemia, ferrofluid actively stimulates cell division predecessors erythropoiesis, verropoulou better than others enhances reticulocyte reaction in bone marrow.

The inventive method of obtaining iron drug differs from the prototype method that allows to obtain products with gemostimuliruyuschee properties of extracts of environmentally friendly natural product of peat in a soft and safe environment.

Sources of information

3. Drugs in Russia. Reference Vidal, 1995, Astra Pharm Service, S. 928.

4. Goldberg E. D., Dugay A. M., Khlusov, I. A. "the Role of the autonomic nervous system in the regulation of Genoese", Tomsk, 1997, S. 218.

1. The method of obtaining iron-containing drug for the treatment and prevention of iron deficiency anemia in animals, which consists in the interaction of organic acids with gemostimuliruyuschee properties and composition of iron with the formation of soluble complexes, characterized in that the quality of organic acids with gemostimuliruyuschee properties apply humic and / or fulvic extracts of peat, and as the iron compounds used water-soluble salts of divalent iron, which take in the amount equivalent to the content of carboxyl groups of humic and / or fulvic acids.

2. The method according to p. 1, characterized in that the humic acid extract of peat allocate by treating it with a solution of hydrochloric acid at pH 1-2, dissolve and carry out the process of complex formation, and the resulting complex - Ferragamo dried.

3. The method according to PP. 1 and 2, characterized in that the extract of peat, after precipitation and removal of guminova the complex - ferrofluid dried.

4. The method according to p. 1, characterized in that the extract of peat containing humic and fulvic acids, is subjected to the process of complex formation, and the resulting complex - verropoulou dried.

 

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