The method of obtaining bone material

 

(57) Abstract:

The invention relates to medicine and can be used to obtain bone material used in dentistry, traumatology and orthopedics. The inventive conduct hydrolysis of milled bone tissue in a mixture solution of 0.4 n sodium hydroxide and ethanol in the ratio 1:1 with constant stirring within 24-48 h, the hydrolysate is drained, bones washed with water, treated with constant stirring, and then heated uniformly increasing the temperature from 600 to 1200oWith, and carry out the firing at 1200oC for 2-5 minutes effect: reduction of antigenicity and improve biocompatibility.

The invention relates to medicine and can be used to obtain bone material used in dentistry, traumatology and orthopedics.

A method of obtaining bone material (SU, patent 5167961), including milling, hydrolysis of bone tissue, washing, drying and calcination in air at a temperature varying between 250 and 600oC.

However, when using this method, you cannot get the bone material similar to native bone mineral.

The objective of the invention is the development of the Ala and increases its biocompatibility.

The technical result is achieved in that in the method of obtaining bone material including milling, hydrolysis of bone tissue, washing, heating and firing, the bone is crushed to the bone crumbs in size from 700 to 2000 μm, carry out the hydrolysis in a mixture solution of 0.4 n sodium hydroxide and ethanol in the ratio 1: 1 with constant stirring within 24-48 h, and then hydrolysat drained, bone chips washed with water, treated with constant stirring until complete neutralization of sodium hydroxide, then heated uniformly increasing the temperature from 600 to 1200oWith, and subjected to firing at a temperature of 1200oC for 2-5 minutes

According to the invention the bone is crushed to the bone crumbs the size of 700-2000 μm, because if the size is less than 700 μm is not optimum leaching of protein, and when larger than 2000 microns leaching of the protein will be difficult.

Hydrolysis of bone chips with constant stirring within 24-48 h is carried out in a mixture of 0.4 n sodium hydroxide solution and ethanol in a ratio of 1: 1, set experimentally to allow complete hydrolysis of the free protein and degrease the bone.

The process of hydrolysis and washing with water purified spend n the LASS="ptx2">

A uniform temperature rise of the heating bone chips from 600 to 1200oWith subsequent firing at a temperature of 1200-1300oC for 2-5 min helps to remove residual proteins (Lowry less than 10 μg/ml). According to laser densitometry, where as the standard selected bone hydroxyapatite received bone material fully contributes to the standard.

The method is as follows.

The bone is cleaned from the muscles, ligaments, and other things, is crushed to obtain bone crumbs the size of 700-2000 μm, followed by hydrolysis in a mixture solution of 0.4 n sodium hydroxide and ethanol in the ratio 1:1, then hydrolysat drained, bones washed with water, treated with constant stirring until complete neutralization of sodium hydroxide in air or in vacuum at a uniform temperature increases from 600 to 1200oWith, and subjected to firing at a temperature of 1200oC for 2-5 minutes

Example. 1 kg bone bull clear of muscles, ligaments, and other things, pulverized in liquid nitrogen to obtain bone crumbs the size of 1900 μm, then stirring constantly, for example, in magnetic stirrer carry out the hydrolysis in a mixture solution of 0.4 n sodium hydroxide and ethanol in zootoxin the Noah stirrer, then heated uniformly increasing the temperature from 600 to 1200oWith, and subjected to firing at a temperature of 1200oC for 3 min in vacuum.

The method of obtaining bone material including milling, hydrolysis of bone tissue, washing, drying and calcination, wherein the bone is crushed to the bone chips ranging in size from 700 to 2000 μm, carry out the hydrolysis in a mixture solution of 0.4 n sodium hydroxide and ethanol in the ratio 1: 1 with constant stirring within 24-48 h, and then hydrolysat drained, bone chips washed with water, treated with constant stirring until complete neutralization of sodium hydroxide, then heated uniformly increasing the temperature from 600 to 1200oWith, and are fired at 1200 - 1300oC for 2-5 minutes

 

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FIELD: surgical facilities.

SUBSTANCE: invention provides material suitable as hemostatic and wound-healing remedy in low-invasive internal surgery involving endoscopic techniques. Allogenic connective-tissue formations (tendons, fascias, dermis) are subjected to mechanical cleaning to remove the rests of adjoining tissues and foreign bodies, washed with running water during 5-10 min, degreased by cold (+4оС) acetone, placed for 5-10 min in 3% hydrogen peroxide solution to remove blood, and thrice rinsed with 0.9% sodium chloride solution. Treated tissues are frozen in cryogenic chamber at -45оС and dried in vacuo to constant weight using liophilic drying technique. Dried material is then wetted at 1:5 weight ratio in solution containing calculated quantities of hemostatic (fibrinogen), fibrinolysis inhibitor (aminocapronic acid), and antibiotic (cephalexin) for 10-20 min until biomaterial structure is completely and uniformly impregnated. Solution id prepared at +4оС by consecutively dissolving 0.2 g cephalexin and 1 g fibrinogen in 10 ml of 5% aminocapronic acid solution. Thereafter, biomaterial is once again frozen and subjected to liophilic drying, then ground on blade-type mill (e.g. "Cyclotec", Foss Tecator) to particle size 0.1-0.25 mm, which are packaged by 0.5 g doses into glass 20-ml bottles, tightly sealed, and sterilized by gamma radiation in dose 2.5 MRad (25 kGr).

EFFECT: simplified technology, improved quality and structure of material.

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