A method of obtaining a concentrate of vitamin raw materials (options)

 

(57) Abstract:

The invention relates to a method of obtaining medicines from plant materials and extracts for the food industry. The extraction is carried out crushed rosehips water in the presence of the enzyme, phase separation, preconcentration and separation. As the enzyme used pictometry GH in the amount of 0.04 to 0.05%, or pectofoetidin GH in the amount of 0,03-0,04%, or cellovyridin GH in the number of 0,06-0,07% of the weight of the swollen material. The extraction is carried out crushed rosehips water, add the enzyme pictometry GH in the amount of 0.03 to 0.04% by volume of the extract, carry out phase separation, preconcentration and separation. The invention improves the yield and quality of the product. 2 S. p. f-crystals, 1 table.

The invention relates to chemical-pharmaceutical industry, as well as related sectors of the food industry, in particular to a method for producing a concentrate of vitamin raw materials, hips, allowing to obtain medicinal syrups, dry extract and the extract for the manufacture of soft drinks.

A known method of obtaining a concentrate of the hips, including extrac the act, a pectinase, produced by the fungus Aspergillis niger, with glucosidase activity, filtration and evaporation /1/.

The disadvantages of this method is the fact that this enzyme is not currently available. In addition, this process is characterized by high concentration of enzyme preparation and the duration of enzymatic lysis by.

Also known is a method of obtaining an extract of rosehip, including fermentors the crushed material using multienzyme composition having cytolytic, amylolytic and pectolytic activity, division of solid and liquid phases, adding ethyl alcohol, settling and filtration /2/.

The disadvantages of this method are: the complexity of obtaining multienzyme composition of multienzyme preparations and their increased concentration, prolonged exposure of preferment on the processed raw materials, rework extract alcohol for his clarification that characterizes the inefficient enzymatic effects on macromolecular compounds raw materials and affects the quality reduce the viscosity of the extract and its OSVETLENIE.

Closest to the proposed method of the invention is a ski enzyme and solution of sulphurous anhydride, separation of solid and liquid phase, evaporation of the extract and filtering /3, 4/, which was chosen as a prototype.

The disadvantages of this method are: high concentration of pectolytic enzyme and sulfur dioxide, have a toxic effect and the complexity of its preparation, prolonged exposure of the enzyme raw materials, the narrow temperature range and, in our opinion, large loss with meal associated with a single contact of the raw material and the extractant.

The task of the invention are: to increase the yield of extractives and quality of plant extract, as well as the simplification of the production of concentrate.

This objective is achieved in that in the method of obtaining a concentrate comprising Fermentas raw materials pectolytic enzyme in the presence of sulfur dioxide, separating the solid and liquid phases, evaporation and filtration, according to the invention is used according to the pattern of enzyme complexes with pectinase-cellulase or cellulase-pectinase activity when exposed to the recovered dry raw material or water extract obtained after extraction of raw water without enzymatic lysis by/.

This allows in the viscosity of the obtained extract and reaching its effective lightening/, to reduce the duration of the technological process, to simplify the process and improve working conditions.

The method is implemented as follows: the crushed rosehips extracted with purified water at a temperature of 50-60oC for two hours, after which the reaction mass is acidified with 1% hydrochloric acid to a pH of 3.8-4.0 and make it activated enzyme complex with pectinase-cellulase action with a concentration of 0.04-0.05% or cellulase-pectinatum effect concentration 0,06-0,07% by weight of recovered raw materials (swollen raw materials) and are fermentors at the above temperature for 1.5 hours. At the same temperature, a further two extraction of raw materials purified water. The extraction process are on the scheme, which consists of two reactors, the first extract from each reactor is directed to evaporation at a temperature of 45-50oWith residual pressure 0,90-0,95 kg/cm2and then sephirot (filtered, centrifuged). The second and third extracts from each reactor is used for the extraction of raw materials in the first and second contact phases in the other reactor, and a third extraction in each reactor serves purified water.

The degree of evaporation of EXTRACTA of kolossa concentration lead to a solids content of 25%, for the production of syrup hips and dry extract of rose hips - up to 15-20%, for the preparation of soft drinks to concentrate with the desired solids content add alcohol to a concentration of 20%.

Physico-chemical properties of rosehip extract, obtained after enzymatic treatment, are presented in the table.

The efficiency of enzymatic lysis by confirmed by the decrease of kinematic viscosity of vegetable extract 1.3-1.6 times, while the percentage of light transmission is increased 5-8 times. The consumption of enzyme preparation is reduced to 20-22 times. The total duration of the extraction of raw materials is reduced by 1.6 times, and enzymatic lysis by 5 times.

Enzyme complexes pectinase-cellulase and cellulase-pectinase steps used for the clarification of aqueous extract, obtained without the use of an enzyme, a concentration of 0.03-0.04% of the volume of the extract.

The proposed method is illustrated by the following examples:

Example 1. The crushed rosehips in the amount of 45 kg extracted with purified water under stirring for two hours at a ratio of solid and liquid phases of 1:10 and a temperature of 45-50oC. Then the reaction and stirring contribute pre-activated enzyme complex pictometry GH with a concentration of 0.05% by weight of recovered raw materials and carry out the enzymatic hydrolysis for 1.5 hours with periodic mixing every 10-15 minutes 2-3 minutes. After the specified time merged the first extract in the amount of 460 l and is directed to the process of evaporation. At the same temperature, a further two extraction of raw water in an amount equal merged the previous extract, the duration of the second attractie is 1 hour, and the third - 0.5 hours. Merge 460 l of the second and third extract. The process of extraction and enzymatic lysis by raw materials is carried out in two reactors, the first extract from each reactor alternately output to the next stage of the process - the process of evaporation. The second extract from the reactor 1 are served on fresh raw materials /first extraction/ reactor 2, add purified water to the ratio between solid and liquid phases of 1: 10 and lead extraction of raw materials and subsequent fermentors under the same process parameters as in the reactor 1. After the specified time is drained first extract from the reactor 2 in the amount of 460 l and send it to the next stage of the process - the process of evaporation. For the second extraction of raw materials to the reactor 2 serves the third extract from the reactor 1, the third - loci of the reactor 2, which is used for the extraction of fresh raw materials in the reactor 1 according to the above scheme. First rosehip extract from each reactor evaporated to 68 l and sephirot. Get water concentrate with a solids content of 25%, which is used to obtain kolossa corresponding to OD.

Example 2. The method is carried out as in example 1, characterized in that the enzymatic lysis by raw materials use enzyme complex pectofoetidin GH with a concentration of 0.04% by weight of recovered materials in the conditions of the above-described process. Get water concentrate (85 l) with a solids content of 20%, which is used for rosehip syrup corresponding to OD.

Example 3. The method is carried out as in example 1, characterized in that the enzymatic lysis by use of the enzyme complex - cellovyridin GH with a concentration of 0.07% by weight of recovered raw materials (swollen raw materials) in terms of the above process. Get water concentrate (85 l) with a solids content of 20%, which is used to obtain a dry extract of rose hips, the corresponding LP.

Example 4. Aqueous extract of rose (first extract) in an amount of 100 l, obtained according to the scheme, PR is under stirring contribute pre-activated enzyme complex pectinase-cellulase action pictometry GH in the amount of 0.03% by volume of the treated extract. Fermentas of the extract is carried out for one hour at a temperature of 45-50oC. the Extract during hydrolysis stirred every 15 min for 2-3 minutes After the specified time, the extract was filtered, evaporated to 15 l and sephirot. Get water concentrate hips with a solids content of 25%, the percentage of light transmission of 75% and a kinematic viscosity of about 2.5 times less.

The use of the proposed method in comparison with known provides the following benefits:

1. A more complete (exhaustive) extraction of active substances from raw materials, which is achieved by carrying out the extraction and enzymatic lysis by under the special scheme at least the duration of the process. The yield of extractives at this stage is 95%.

2. Reduced enzymatic hydrolysis of not less than 5 times.

3. Lower consumption of the enzyme preparation in 20-22 times when high quality water concentrate.

4. Reducing energy consumption by reducing the duration of the process.

5. Simplifying the process and reducing labor costs for ristoro gas in water to obtain a solution of desired concentration. The hydrochloric acid solution is prepared by simple mixing (13 ml concentrated acid and purified water up to 540 ml).

6. The improvement of environmental safety of the process by eliminating the use of sulfur dioxide.

7. Over a wide temperature range in which most effective enzyme preparations.

The developed method was verified to sue the SEC VILAR" in an industrial environment when processing four batches of raw materials (total weight of over 2 tons) in 1997-1999 and showed the above results.

On the basis of the proposed method is currently being drawn up PR for the production of an extract from the fruit of the wild rose.

Sources of information

1. Ant I. A. Technology of drugs. M., 1980, I. 1, pp. 215-220.

2. Filonova, L. "Development of technology for production of extracts of plant materials to improve the quality of non-alcoholic beverages". Abstract. Kida. dis. M., 1982

3. Novoselov N. In. and Deeva Century, that is, the Method of obtaining concentrates of vitamin C. Ed. testimony 62604, a 61 K 35/78.

4. Ignatiev B. D. Rose and its use. Novosibirsk, 1946

1. A method of obtaining a concentrate of vitamin raw materials, including the extras is the formation and separation, characterized in that the enzyme used pictometry GH in the amount of 0.04 to 0.05%, or pectofoetidin GH in the amount of 0,03-0,04%, or cellovyridin GH in the number of 0,06-0,07% by weight of recovered raw materials.

2. A method of obtaining a concentrate of vitamin raw materials, including extraction of the crushed rosehips water, adding enzyme, separating the solid and liquid phases, concentration and separation, characterized in that pictometry GH in the amount of 0.03-0.04% of the volume of extract added to the resulting aqueous extract.

 

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