The antidote biological poisons snakes

 

(57) Abstract:

The invention relates to medicine, namely to methods provide emergency self - help and mutual aid and can be used to prevent complications from snake bites in field conditions. The antidote contains a set of inhibitors of components of the venom, such as the venom of the Central Asian Cobra twin, zinc Valerianovich, caffeine, prednisolone, -aminocaproic acid, Riboflavin, neostigmine, glutathione and heparin, the antidote is injected into the tissue directly in the hit area for the poison immediately after a sting. The antidote can reduce complications and mortality in bites and more available for the provision of self - help and mutual aid.

The invention relates to medicine, namely to methods provide emergency self - help and mutual aid and can be used to prevent complications from bites and Ugolini poisonous insects, arthropods and snakes in the field.

Known antidotes when the snake bites: "Antihorse", "Anticore" and other Disadvantages are possible anaphylactic reactions, allowing their use only in stationary conditions [1, 2].

This method was adopted for the prototype.

their possible complications of medical care, of morbidity and mortality in case of bites and walayah.

This goal is achieved by introducing antidote containing complex inhibitors of components of the venom, such as poison sredneaziatskoi Cobra twin, zinc valerianaceae, caffeine, prednisolone, -aminocaproic acid, Riboflavin, neostigmine, glutathione and heparin, the antidote is injected into the tissue directly in the hit area for the poison immediately after the sting.

Comparative analysis of the claimed invention with the known solutions shows that they are significantly different according to the type of substances related to inhibitors of components of snake venom, and procedures that involve introducing them directly into the tissue in the area of the bite (sting) to themselves and others.

The biological feasibility of this methodological admission is based on the fact that the main components of snake venom proteins and peptides, which accounts for about 80% of its dry weight - are carriers of the most toxic and enzymatic properties of the poison.

Many poisons and their fractions studied by elemental and amino acid composition. It is established that the toxicity and some enzymatic properties Zmeev Cobra Viper Russell, rattlesnake, almost completely eliminating their clotting effect on the blood and phospholipase activity of the latter two poisons. Enzymes from snake venoms inhibits EDTA, binding calcium ions and preventing enzyme opportunities to form with it a complex with micelles of the substrate, and zinc ions [2].

The presence poisons the following components: phospholipase A2, phospholipase B, phospholipase, hyaluronidase, cholinesterase, phosphatase, alkaline phosphomonoesterase, 5-nucleotidase, phosphodieterase, ATP-ASE, proteolytic enzymes, oxidase-1-amino acids, coagulants and anticoagulant, and, in addition, knowledge of their properties and the presence among pharmacopoeial funds inhibitors of these components [2, 3], allowed us to develop a comprehensive antidote.

Examples of practical implementation of the method

The experiments were conducted on 48 outbred rats of both sexes weighing 130-150 g Rats in the thigh was injected venom of the Central Asian Cobra dose 0,067 mg/100 g (LD80). Then immediately after injection of the poison through the same needle without changing its provisions were introduced, we offer you the antidote.

With the introduction of antidote revealed an increase of survival rate (25-33%, and the time of onset of about the conclusion about the prospects of this approach chelation treatment of various etiologies, and, in practical terms, the possibility of creating a syringe-tubes used to reduce deaths when the snake bites, and prevent (reduce the severity of symptoms of intoxication.

Thus, the application of this non-toxic and non-allergenic antidote as an emergency means of self - help and mutual aid will reduce possible complications, morbidity and mortality in bites and walayah. In addition, delaying the onset of deterioration of the General condition will produce the most rapid evacuation to a treatment facility on their own.

Sources of information

1. Orlov N. N., Gelashvili D. B., Ibragimov A. K. Poisonous animals and plants of the USSR. - M.: Higher school, 1990. - 272 S.

2. Orlov N. N., Valcea I. A. Poisons of snakes. So: Medicine, 1977. - 252 S.

3. Sakhibov D. N., Sorokin, C. M., Kelson L. J. Chemistry and biochemistry of snake venoms. So: Fan, 1972. - 185 C.

Enticed biological poisons snakes, characterized in that it contains a set of inhibitors of components of the venom, including twin, zinc valerianaceae, caffeine, prednisolone, -aminocaproic acid, Riboflavin, Preseren, glutathione and heparin, the antidote is injected into the tissue directly in the area of p is

 

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