The tool hydrosulfate, grafito-metal complex and method for the treatment of skin diseases (variants)

 

(57) Abstract:

The invention relates to medicine, namely oncodermatology, medical cosmetology, pharmacology. The invention lies in the fact that the proposed product contains the hydrosulphate of gravito-metal complex obtained by the processing of graphite with concentrated sulfuric acid in the presence of concentrated nitric acid, which dissolved copper and zinc. Ointment form means further comprises a filler having colloidal properties, at a certain ratio of filler and chemical complex. In addition, a method of treatment of patients with neoplastic, inflammatory and trophic skin lesions by applying a liquid or ointment form of this tool on the affected skin. The invention provides sinergicheskoe biological effect on all skin structure - immuno-phagocytic system, epidermis, connective tissue, the microcirculation and lymph circulation, with the restoration of homeostasis in tissues of the skin and subcutaneous tissue and simultaneous modeling of tissue and cell apoptosis in the occurrence of a particular type of tumor. 4 C. and 1 C.p. f-crystals.

The invention otoyti used in clinical medicine at the same time as for elimination of benign and malignant skin tumors (papillomas, melanoma, pigmented nevi, hemangiomas, squamous cell skin cancer and other tumor entities) and for the treatment of patients with various inflammatory and trophic lesions involving tissue autoimmune reactions with the formation of keloid scars.

Currently in oncodermatology and medical cosmetology uses a variety of tools to remove the tumors, the normalization of trophic processes in order to activate blood circulation, restore the function of the immune and lymphatic systems. For example, the treatment of patients with tumors of the skin is carried out by chemical methods the impact of using concentrated solutions of sulphuric, nitric and acetic acids and liquid nitrogen. At the same time the destruction of tumors acids is often accompanied by disruption of the structure of the connective tissue matrix of the skin and the formation of a cosmetic defect. Inflammatory processes are mainly applied antiseptic and cauterizing agents, antibiotics, anti-inflammatory, disinfectant, antibacterial and keratolytic substances in the form of ointments, gels, creams and other products that have beneficial therapeutic effect (O. S. Lakes the ranks forms of skin diseases requires the use of diverse drugs, often incompatible with the immune and vascular systems. It should be noted that the skin of a human body is a complex biological tissue system in which immune processes synergiczne connected to the microcirculation and lymphoblastoid.

In implementing the immune activity of the skin, the main role belongs to the T-populations of lymphocytes, tissue histiocytes and Langerhans cells. These dendritics cells located in the epidermis and represent approximately 5% of the total number of epidermal cells. This population of macrophages has a characteristic feature, because due to the long processes of these cells increases the area of reactivity and enhanced immune response. Langerhans cells are the most important part of the system of antigen-presenting cells, because delay the antigens enter the body, and present these foreign proteins to T cells to generate an immune response. Although dendritics cells are deprived of additional signals (SD, SD and CD86) to activate T-cells, they are well adapted to capture antigens that induce full maturation and mobilization of this cell population. For the generation of cytotoxic killer cells with sposobnosti complex antigenic peptides and molecules of the first class major histocompatibility complex T-cells, expressing the CD8 antigen. A critical factor in triggering the immune response is the maturation of Langerhans cells. It can be triggered by various stimuli, including microbial antigens and products of inflammation. Mature and differentiated dendrites cell can effectively activate T cells, after which they can make an adequate immune response. Thus, along with participation in phagocytic reactions, dendritics cells carry out antigen presenting function, which contributes to the activation of T cells and tissue histiocytes. It should be noted that the contact dendritics cells with molecules CD40 leads to secretion of IL-12 and other cytokines.

In experimental studies on animals have shown that dendritics cells incubated with tumor antigens capable of generating the formation of T-cell protective response against tumor cells, re-introduced into the body, and against existing tumors (Century. Century. Ptuskin "Dendritics cells and the role of cytokines in the differentiation and function" in kN. "Clinical Oncohematology", M.: Medicine, 2001, S. 72-76).

Tissue histiocytes (macrophages) ability to intense immune response. The interaction of T cells with histiocytes dramatically increases their bactericidal activity. On the surface these tissue histiocytes, like other macrophages have receptors for immunoglobulin and complement. Along with intracellular digestion of antigen histiocytes produce factor to increased phagocytic activity.

Thus, the immune system of the skin is largely reactive-phagocytic and presented by Langerhans cells and tissue histiocytes.

The immune system of the skin localized in the epidermis and in the dermis, which makes the unbreakable bond between the populations of immune cells with the structure of the connective tissue. Morphological features of the structure of the skin open to the possibility of performing its various immunologic reactions, showing that the ability to anamnestic response and at the same time participating in barrier protective functions.

Average or connective tissue of the skin - the dermis - takes the bulk of the skin. It is built of collagen, elastic and argyrophilic fibers arranged in a generally amorphous substance. In the dermis are fibroblasts, histiocytes, mast cells, melanocytes and leukocytes. Basics water-related. Base material binds the fibrous skeleton of the cellular elements and fibrillar proteins.

Microcirculation of the skin presents superficial and deep vascular networks. The superficial vascular plexus is located under the basal membrane and performs gas exchange and nutrient functions. Deep vascular network is on the border of the subcutaneous fatty tissue and consists of arterial-venous plexus, poor capillaries, which eliminates the possibility of an intensive exchange of substances between blood and tissue. With the circulatory system of the skin is closely related and lymphatic system, which consists of a surface network and subdermal lymphatic plexus, located on the lower border of the skin.

Received to date data histophysiology and pathomorphology of the skin suggests that cellular immune education (dendritics cells, tissue histiocytes and fibroblasts) in conjunction with the collagen of the skin, vascular and lymphatic networks combined into a single biological mechanism. In this regard, a fundamentally new ways of treating these or other drugs should combine simultaneous sinner is ecene benign and malignant skin tumors as well as inflammatory and trophic disorders is based on the use of such medicines and at the same time the chemical substances, which will be synergiczne to act on immune mechanisms, connective tissue and microcirculation of the skin with preservation of structures of the epidermis, the basement membrane, blood and lymphatic systems. This substance should be presented component transdermal permeability, substance effects on connective tissue and the immune system, and means to regulate the process of apoptosis in tumor, inflammatory and trophic diseases of skin.

When applying a variety of medicinal substances inevitably raises the question of the intervention of certain drugs in the process of homeostasis, which includes such phenomena as cell proliferation, tissue differentiation and cell death, which is an important component in the preservation of fundamental constants homeostasis.

It is known that the most common form of cell death is necrosis, which is caused by a variety of factors damage the cells. However, there is another form of cell death called programmed cleocin the Cam (A. Y. Baryshnikov "Programmed cell death (apoptosis) in kN. "Clinical Oncohematology", M.: Medicine, 2001, S. 36-42). Morphologically, apoptosis at the cellular level should be manifested by shrinkage of the cell, reducing its size and condensation of the chromatin at the periphery of the nuclear membrane. Unlike necrosis, apoptosis is evident asynchronously in scattered individual cells and is not associated with inflammation. The tissue form of apoptosis combines all the hallmarks of apoptosis with simultaneous involvement of the microvasculature (ischemic effect) and the destruction of the connective tissue matrix is primarily collagen structures. It should be noted that tumor growth is the result of imbalance between cell proliferation and programmed cell death.

The process of apoptosis can be divided into three independent phases: initiation, effector phase and degradation. The initiation phase can be achieved in various ways, including removal of growth factors, hypoxia, and subsections lesions resulting from the application of various chemical reagents. In the effector phase of tissue forms apoptosis play an important role in ischemic factors and immune-egress is ctur and homeostasis. Phase degradation is the completion of the apoptotic process and is an integral part of the rejection of alien structures.

In modeling the mechanism of apoptosis is the main role belongs receptore-ligand system SD. Structural organization of antigen CD makes it a member of the superfamily of TNF-receptor molecules, including two types of receptors of tumor necrosis factor, nismapentry nerve growth factor, T-cell activation antigen SD, b-cell antigen CD.

Presents brief information about the mechanisms of the simulation cell and tissue apoptosis indicate the complexity of the biological phenomena and clearly indicate the relationship of the so-called programmed cell death by homeostasis, which involves both the development and the degradation of tissues and cells.

Research conducted by pharmacochemical and homeopathic clinicians showed that some aggressive acids, for example sulfuric, have a high penetrating power through the epidermis and dermis, and therefore they are used to eliminate cancerous ulcers, boils, and various inflammatory processes. In homeopathic medicine the substances UE is s (James Tyler Kent, Lectures on homeopathic medicine Materia Medica, so 2. "Homeopathic medicine", Moscow, 1999, S. 1248-1253). Therefore, aggressive acids can be used for modeling transdermal permeability.

Medicine elective acting on connective tissue and immune-phagocytic system of the skin, graphite is widely used in homeopathic medicine as a means skin, lymphatic and venous (G. Keller, Homeopathy, Moscow, 1993, C. 247,252,260, 263). The use of graphite in homeopathy has revealed its selective action on the skin tissue, including directly onto the skin surface, as well as on the blood and lymphatic capillaries. This therapeutic drug has profound therapeutic effect and is used for elimination of malignant tumors, cancerous ulcers and chronic scarring of the skin.

Pharmacodynamic properties of graphite show that it is able to penetrate through the microcirculation and the lymphatic system in various structures of the skin. Along with the antitumor effect of graphite have been identified and its anti-inflammatory properties (especially when the erysipelatous lymphangitic), which indicates that direct stimulation of them the time, a significant drawback of this method is the inability of finely powdered graphite - powder or granulated to penetrate deeply through the capillary and lymphatic system of the skin in the microcirculatory bed.

The recent data on immunopharmacology trace elements showed that copper and zinc are directly involved in the regulation of apoptosis and the formation of immune reactions. At the present time can be considered definitely established fact that copper and zinc are both as mediators of apoptosis and directly participate in the formation of immune responses. (Kudrin, A. C., Rocky A. C., lark A. A., Rock, M. G. , Gromova, O. A. "Immunopharmacology trace elements", Moscow, 2000, S. 228-277).

Copper, being one of the indispensable essential trace elements, essential for human life because of its necessity for the synthesis of ceruloplasmin. Ceruloplasmin, as you know, is involved in the provision of basic homeostatic processes of the liver, heart, muscle, skin and other tissues. This is essential to the body protein is also involved in the degradation of biogenic amines and simultaneously performs a critical function in stimulation of cells of the immune system - CD4 - helper cells, b-lymphocytes and macrophages. Provoditsya cellular and humoral immunodeficiency. Recently, there is evidence that copper deficiency dramatically disrupts the regulation of homeostatic processes, which leads to damage of blood vessels, liver, blood and the development of tumors. Thus, the copper as the mediator homeostasis and non-specific cofactor immune processes simultaneously carries out the development and proliferation of tissues with subsequent biologically programmed cellular and molecular death.

An even more important role in the formation of apoptosis and immune responses plays zinc is required for normal functioning of every cell. It is proved that the treatment zinc potentiates cell mediated immune response against bacteria, viruses and tumor cells. Zinc deficiency leads to impairment of phagocytosis and T-cell responses. Now clearly established that zinc is one of the important intracellular mediators of apoptosis and is required for all homeostatic processes, because this trace element associated with the synthesis and DNA repair, embryogenesis, tissue regeneration, immunogenesis, the functioning of the endocrine and nervous systems.

Each of the above substances separately has certain Phi actions on the immune-phagocytic system, connective tissue structures of the skin, as well as on microcirculation and lymph.

The present invention is to remedy these disadvantages and create tools that provide simultaneous biological effect on the whole structure of the skin-immune-phagocytic system, epidermis, connective tissue, the microcirculation and the lymphatic circulation with the restoration of homeostasis in the skin tissues and simultaneous modeling of apoptosis in the occurrence of a particular type of tumor. In this regard, the medicinal substance can be used in the treatment of various skin diseases - neoplastic, inflammatory and trophic.

To achieve the objectives of the proposed group of inventions, United by a common inventive concept. For the treatment of skin diseases includes chemical composition C24+HSO4-H2SO4- hydrosulphate of graphite, in which the substance is engaged permeability is sulfuric acid, and therapeutic active impinging molecules of graphite. The graphite in this connection limits the action of sulfuric acid and at the same time synergiczne with sulfuric acid, the us copper and zinc, obtained by dissolving one gram of each metal in 50 ml of concentrated nitric acid. This substance is called the hydrosulphate of GRAFITO metal complex obtained in the processing of graphite with concentrated sulfuric acid in the presence of oxidant - concentrated nitric acid, which dissolved 1 g of copper and 1 g of zinc. This chemical substance is a compound inclusions of graphite in sulfuric acid and is formed by the introduction of atoms, ions or molecules between the carbon layers of the crystal lattice of graphite. As is known, the distance between carbon atoms in the layer of graphite is of 1.42 A. the Link between carbon atoms in one plane are covalent in nature. The interlayer distance between carbon atoms more at 3.35 A, and the connection between the interlayer carbon atoms is much weaker. In these interlayer voids graphite and can, under certain conditions, to diffuse the atoms and molecules of some compounds, including molecules of sulfuric acid. At different time intervals can be embedded variable amount of sulfuric acid, but in the limit formed by the compound (C24+HSO4-H2SO4) containing 1 g of graphite 1 g of sulfuric kisli solution. Graphite has a density equal to 2.2 g/cm3; concentrated sulphuric acid is 1.84 g/cm3. Forming a liquid has a density equal 1,815 g/cm3.

In addition, the proposed remedy for the treatment of skin diseases containing the hydrosulphate of gravito-metal complex and a filler having colloidal properties, at a volume ratio of the filler and complex 25:1-12,5:1.

In the treatment of skin diseases involving tumor formations in the affected areas are exposed to hydrosulfate, GRAFITO metal complex containing 1 ml of sulfuric acid and 20 mg of copper and 20 mg of zinc. The liquid is applied in a dose of 0.05 ml and the surrounding tissue smeared with vaseline. Large skin lesions treated two or three times with an interval of 10-20 minutes; Ceratophyllum and pigmented nevi process two or three times with an interval of three days within 2-6 weeks. In addition, a method of treatment of skin diseases involving inflammatory and trophic disorders, characterized by the fact that in the affected areas causing the same tool (the above chemical complex) in ointment form. With slightly pronounced skin lesions using the tool in the bulk with the diversified scars, 12,5:1.

Examples of receiving liquid and ointment forms of hydrosulphate of gravito-metal complex.

Example 1. Obtaining a liquid form.

In a glass container with a capacity of 700 cm3make a volume of 100 cm3graphite, pour in 500 ml of concentrated sulfuric acid solution by adding 50 ml of concentrated nitric acid, which dissolved 1 g of copper and 1 g of zinc. Tightly sealed and put in a warm dark place, withstanding chemical ingredients at a temperature of 20oWith in a month. Upon expiration of the above period, the liquid becomes intense brown color blends into another container, tightly corked and stored in a dark place at normal room temperature.

Example 2. Getting the wax forms a hydrosulfate, GRAFITO-metal complex.

To 25 g of vaseline or any other ingredient having colloidal properties and application in medical cosmetology, add 1 or 2 ml hydrosulfate, GRAFITO-metal complex (volume ratio vaseline or any colloidal substances to chemical complex is 25:1 or 12.5: 1). The contents thoroughly mixed glass blockades matter. After this ointment, which is a mixture of vaseline and this chemical complex, tightly corked and placed in the refrigerator for sealing the funds received.

Examples of the method of treatment of skin diseases

Example 1. Patient E. G., 44 years. Diagnosis: Toxic-allergic dermatitis of the skin of the finger on the right hand and the rear of the right foot.

Ill for 3 years, when they discovered the inflammation of the skin of the outer surface of the index finger of the right hand. The disease initially had a transient nature, and skin changes were noted only after contact with detergents synthetic means. A year later, the inflammation of the skin has become aggressive in nature and almost all of the outer surface of the skin of the index finger of the right hand were thinning and destruction, so that in some places, exposing the subcutaneous fatty tissue and there were numerous bleeding cracks in places of destruction. A similar phenomenon is less pronounced, were observed in the skin of the dorsal surface of the right foot. Carried by biocosmetics the treatment of various inflammatory and hormonal therapy were not able to eliminate the disease, although the patient is the definition of dermatitis.

During the inspection a clear picture of destruction of the skin of the outer surface of the right index finger with numerous cracks in some places marked exposure of the subcutaneous fat. Affected areas of skin, bleeding in these areas felt the pain and phenomena unbearable itch.

With the consent of the patient treatment ointment form the hydrosulphate of gravito-metal complex (volumetric ratio of the substance and filler 1:25). On parts of the skin of the finger and the back surface of the foot was applied a thin layer of ointment twice a day (morning and evening) with a duration of 1 and 2 hours. A week later the patient noted a decrease in itching, and after 2 weeks, the first signs of skin regeneration. The treatment lasted 2 months. During this time there was a full restoration of tissue structure in the region of the skin of the right foot and almost complete restoration of the structure of the skin and subcutaneous tissue in the area of the outer surface of the right index finger, completely disappeared itching and pain. From a preventive point of view was held even one course of treatment. Recurrence of the disease in future.

In the observation of trophic disturbances, which is the trace elements copper and zinc, almost completely gone, which confirms the fact of recovery of tissue homeostatic processes after applying the above chemical complex.

Example 2. Patient E. N., 59 years. Diagnosis: Melanosis Dubreuil skin left temporal region.

Considers himself ill for 3 years, when he noted in his temple the appearance of dark brown spots. Gradually this education had increased and after a year from the onset of the disease was the size of a 5-ruble coin. The patient was referred to an oncologist, who was diagnosed with melanosis Dubreuil and proposed excision of the tumor with subsequent skin graft. Repeatedly visited various beauty rooms where she was given treatment by electrocoagulation method. At the time of treatment in biocosmology center of Rosica" melanosis Dubreuil has developed into a large dark brown tumor that occupied the skin of the whole temporal region. The patient was offered treatment with the use of the hydrosulphate of gravito-metal complex, in which the patient has given consent. Lying on tumor formation within 3 months 1 every 3 days was applied a thin layer of liquid hydrosulfate, GRAFITO-metal complex, the skin around the cheeks sharply turned pale, indicating that it occurs in those areas of the tissue effects of ischemia. The phenomenon of spasm of the microvasculature of the skin around the tumor continued for 2-3 min, after which it was a phase marked hyperemia, which covered the entire half of the head and down the neck up to the clavicle. Lubrication pigment formation was carried out regularly to the complete destruction of the tumor. Elimination of the tumor tissue was gradually decay of her began to form on the periphery and in the next was marked in the center of the tumor growth. By the end of treatment, there was a complete tumor resorption and regeneration of the skin to restore natural color and hair growth. Toksikoallergicheskie reactions during treatment the patient was not observed.

In this case the use of the hydrosulphate of gravito-metal complex containing trace elements of copper and zinc, resulted in the induction of apoptosis of the tumor and its destruction, which is outwardly manifested in severe ischemic effect. In subsequent destruction of the tumor occurred through the tissue of the immune process, which resulted in complete resolution of the tumor. The restoration of the natural structures of the skin confirms the activation of the homeostatic mechanism is 3. Patient W. K. 15 years. Diagnosis: Festering cyst in the upper-outer quadrant of the left breast.

The disease appeared suddenly after suffering a follicular tonsillitis, when the skin of the left breast there was swelling with redness and pain. Ultrasonography was diagnosed with festering cyst subcutaneous tissue of the left breast. From surgical treatment the patient refused.

With the consent of the mother, the patient started treatment ointment form the hydrosulphate of gravito-metal complex (volumetric ratio of the substance and filler 1:12,5). In the area of inflammation in the morning and in the evening was applied a thin layer of ointment for 1.5 hours. During treatment were noted slight burning and tingling in the lesion. After 2 weeks, the inflammatory process in the field of skin cancer and subcutaneous tissue is clinically determined, repeated ultrasound diagnosis stated resorption cysts. Recurrence of the disease in the following did not happen.

This observation inflammation of the skin and subcutaneous fatty tissue, leading to tissue damage and as a consequence, apoptosis, was eliminated by the application of the ointment form hydrosulfate, GRAFITO-meta is tructure affected tissues.

1. The treatment for diseases of the skin, characterized in that it contains the hydrosulphate of gravito-metal complex obtained by treating graphite with concentrated sulfuric acid in a volume ratio of 1: 5 in the presence of concentrated nitric acid, which dissolved copper and zinc, based on 1 g of each of the metals in 50 ml of nitric acid.

2. The treatment for diseases of the skin, characterized in that it contains the hydrosulphate of gravito-metal complex obtained by treating graphite with concentrated sulfuric acid in a volume ratio of 1: 5 in the presence of concentrated nitric acid, which dissolved copper and zinc (1 g of each of the metals in 50 ml of nitric acid), and a filler having colloidal properties at a volume ratio of the filler and the hydrosulphate of gravito-metal complex 25: 1-12,5: 1.

3. Means under item 2, characterized in that the filler it contains petrolatum.

4. The method of treatment of diseases of the skin, characterized in that the induction of apoptosis of tumor formation and the destruction is carried out by applying to the affected areas of skin on p. 1 € 2-3 times at intervals of 10-20 minutes, Ceratophyllum and pigmented nevi 2-3 times with an interval of 3 days within 2-6 weeks.

5. The method of treatment of diseases of the skin, characterized by the fact that in the treatment of patients with inflammatory and trophic disorders use the tool under item 2, if slightly severe skin lesions using the tool containing the filler and the hydrosulphate of gravito-metal complex in a volume ratio of 25: 1, and in inflammatory processes involving keloid scars is 12.5: 1.

 

Same patents:

The invention relates to medicine and relates to a photosensitizer for photodynamic therapy and method of its production, including alkaline salt of chlorin e6, purpurine 5 and chlorin p6characterized the optimal rate of accumulation in the tumor and its removal of the tumor, as well as high stability

The invention relates to the field of medicine and for the application of sulfadimidine besmearing phthalocyanine and its magnesium complex of General formula (I) as a photosensitizer for photodynamic therapy (FDP)

The invention relates to new derivatives of phenanthridine General formula A, where R1substituted or unsubstituted lower aliphatic hydrocarbon group, R is an aliphatic hydrocarbon chain having 2 to 6 carbon atoms, which may be optionally substituted by a Deputy selected from the group comprising lower alkyl group, halogen or a hydroxy-group, each of Y and Z independently represents hydrogen, hydroxy or lower alkoxygroup, or Y and Z together with the formation of methylenedioxy or phenyl ring, and X-- acid residue or a residue hydrogen acid, and a derivative of phenanthridine General formula V, where R1, R, Y, Z are defined above for the formula And
The invention relates to medicine, namely to Oncology, and can be used in the treatment of malignant soft tissue tumors with an unfavorable course of the disease
The invention relates to medicine and for the creation of a means and method for the treatment of patients with benign and malignant skin tumors

Antitumor peptides // 2182911

The invention relates to medicine and relates to a method of destruction of breast cancer cells or other carcinoma cells, preparation for destroying cells and kit for performing the method
The invention relates to medicine

The invention relates to medicine, in particular to cancer pharmacology

The invention relates to medicine

The invention relates to medicine and relates to methods of suppressing excessive secretion of tumor necrosis factor alpha and interleukin-1 beta, as well as the treatment of diseases associated with excessive synthesis of these cytokines, Alzheimer's and Parkinson's disease using the derived indolocarbazole K-252a

The invention relates to medicine and relates to a method of obtaining radioprotector

The invention relates to medicine, namely to dermatology, venereology and medical cosmetology

The invention relates to a composition for topical application in the form of an emulsion for the treatment of inflammatory and hyperproliferative diseases of the skin and cutaneous manifestations of immunologically mediated diseases

The invention relates to solutions for surgical irrigation and methods of use thereof, in particular to anti-inflammatory, analgesic and anticonvulsant solutions
The invention relates to pharmaceutical industry and veterinary
The invention relates to pharmaceutical industry and veterinary
Enterosorbent // 2180577
The invention relates to medicine
Enterosorbent // 2180231
The invention relates to medicine and can be used as enterosorbent
Up!