The way to influence the process photoregulatory activity of biological objects and arrangement for implementing the method

 

(57) Abstract:

The invention relates to Biophysics and can be used in crop production. The use of a composition containing as phosphors compounds of rare earth elements, antimony, tin, manganese, boron, organic compounds or oxides with lifetimes of luminescence 10-12- 10 effective amount of 0.001 - 10 wt. % in the irradiation of biological objects pulsed light output provides increased utilization of solar energy bio-objects in the process of photosynthesis. 2 s and 5 C.p. f-crystals, 1 table.

The invention relates to Biophysics, specifically to methods of exposure to light of certain biological structures to encourage them in photochemical reactions, and can be used to stimulate biological processes in plants and animals, as photosynthesis, vision, photoreactivity, as well as for the reactions of photoperiodism, phototropism and phototaxis, photomorphogenesis, pattinase.

The problem of the biological effect of various physico-chemical factors in midget doses, including therapeutic effect on a living organism of low-intensity radiation with nebritogo thread today is one of the priority directions in physiology, and botany.

The transformation of light energy into various photobiological processes includes such stages as the absorption of light in biostructures, the migration energy and the excitation of the photoactive chromophore complex photophysical the act of transforming the electronic energy and charge separation, and the development of the resulting biological effects. In mammals and plants have similar systems of perception of light, performing regulatory functions, the main features of phytochromic system of regulation which are: high sensitivity to light; conjugation system with cellular membranes; control over the synthesis of DNA and RNA, protein.

Known effects of low-intensity fluorescent radiation on the hemoglobin of the blood vessels of the skin (M. M. Asimov, P. M. Asimov, L. I. Rubies. J. go active. spectroscopy, T. 65, 6, 1998, S. 877-880), as well as the strengthening of bioenergetic processes in the skin while stimulating them with low-intensity He-Ne laser (C. M. Zubkov. Biological Sciences, 7, 1978, S. 32-36).

It is also known that the rate of synthesis of nucleic acids Cultura not exceed 10-2J/cm2and the power density of the light flux not exceeding 1.25 mW/cm2(I. C. Bulakov. The influence of the radiation of helium-neon laser on the growth and development of newborn rats. - Reports of the Academy of Sciences, 1998, I. 358, 1, S. 130).

However, the application of laser sources for the implementation of such influence on metabolic processes in the body has several disadvantages: first, as a rule, it is not always possible to achieve the required small densities the power flow in concurrent excessive monochromatization, and secondly, it requires a fairly complex and expensive equipment, which involves the use of it in the hospital and provides, as a rule, point impact on the individual elements of the bio-object, which is not always justified.

In photosynthetic organisms, light energy is first absorbed by the colored pigment cells (chlorophylls, carotenoids: -carotene, -carotene, luteal, violaxanthin, fucoxanthin, phycoerythrine, phycocyanine and others ), then transported by him, is converted in the reaction centers in the energy of separated charges, stabilizes in time for effective interfacing with slow biochemical stages, produces MoLSA part of the pigments only absorbs the light and transfers the excitation energy in the photochemical centers. In all known cases, the primary photochemical process is to transfer electrons from chlorophyll to any electron acceptor.

Currently composed of the so-called Z-scheme of photosynthesis, from which it follows that the efficiency of transport energy to the reaction centers at various stages of photosynthesis is characterized by certain times of transfer, i.e., each photochemical center should have some time to complete, since each molecule is in the excited state for a certain time, and thus to carry out photochemical molecule can act only time that are characteristic of the excitation (ORT D, Govindjee, Whitmarsh j. and other Photosynthesis. M.: Mir, so 1, 1987, S. 728).

In the light stage of photosynthesis various stages of electron transport are characterized by intermittent transfer from 1 PS to a few seconds. For example, the oxidation reaction center chlorophyll R in the first stage proceeds in a relatively short time (1 NS). Oxidation and reduction of cytochrome - secondary vectors of electrons during photosynthesis can be recorded on the change of light absorption in the range of 400 to 430 nm and 550 - 560 nm for a time of about 1 msaada in sugar for S. 0,01

Also has the effect of impulseto light on the magnitude of the quantum yield of photosynthesis of Chlorella (Borodin Century B., Vavilov, M. J., bell, L. N. - Biophysics, I. XXVII, V. 2, 1983, 280-283 C.).

Well enough studied the effect of spectral composition and intensity of photosynthetically active radiation (PAR) on the growth, development, direction, biosynthesis, photoregulation and other processes affecting the formation of the most important components of plant biomass. However, it is shown that in these processes the efficiency of photosynthetic active solar light does not exceed 1% (D. Hall, K. RAO. The photosynthesis. - M.: Mir, 1983, S. 96).

At the moment the actual objectives are to increase the efficiency of photosynthetic active part of the light energy and the development of new ways of intensification as a production process of the plant as a whole and the individual components of the process photoregulatory activity of biological objects, such as photosynthesis, with a wide variation of spectral characteristics (intensity and kinetics of radiation) radiant flux.

Widespread way of influencing photoregulatory processes of plants, which plants the F. I. Sidko. The spectral composition of light and plant productivity. - Novosibirsk: Nauka, 1991, S. 166).

The number of known ways of influencing photoregulatory activity of plants by cultivation in the conditions of irradiation with light of fluorescent lamps, providing a certain part of the light flux.

For example, for growing seedlings with a long development cycle using fluorescent lamp low pressure with phosphor, comprising 70-95 wt. % Y2ABOUT3:Eu,b with a red glow and 5-30 wt. % BaO2MgO8Al2O3:Eu blue glow (p. RF 1677743, publ. 15.09.91).

Known to use several fluorescent lamps that provide different spectral composition of light in the field light for regulation of growth and photosynthetic reactions when growing tomatoes (a.c. CCCP 1754021, publ. BI. 30, 1992).

However, when using fluorescent lamps and fluorescent lamps consumes large amounts of electricity, and it also creates a luminous flux with a fairly wide frequency range and significantly different in composition from sunlight.

Closest to the claimed method is impact on the process batorego is the obtained by using mechanical devices (D. Hall, K. RAO. The photosynthesis. - M.: Mir, 1983, S. 29). Flashes of light, the duration of about one millisecond (10-3(C) receiving, by placing in the path of the beam constant light rotating disk with a slit or electrically charging the capacitor and discharging it through the vacuum tube. Use and lasers.

However, the method is technically very difficult to perform, requires energy consumption, and does not allow to achieve the required small time between outbreaks.

Known composition PA-based thermoplastic polymer containing a mixture of phosphors, giving a luminous flux, the composition of which is close to the LIGHTS (p. RF 2125069, publ. 20.07.98).

However, the composition due to the use of compounds having a low coefficient of conversion of sunlight, especially in the area of the HEADLIGHT, not effective enough.

Indeed, all inorganic bases, in which ions are introduced activators, in the known solution has absorption bands in the wavelength 150-250 nm (Hoefraad H. E. J. Solid State chem., 1975, v.l5, R. 175; Poluektov N. S. , L. Kononenko I. Spectrophotometric methods for the determination of individual REE. 1968), missing atobatele radiation only in the spectral range of 150-300 nm. At the same time it has a very low coefficient of conversion of light energy in the spectral range of >300 nm, since in this spectral range are all used within the inorganic matrix is optically transparent and absorb only the ions of the activator Eu2+, UOM (111) having a low extinction coefficients, /=1-100/ (Poluektov N. C. Spectrophotometric methods for the determination of individual REE. Kiev: Naukova Dumka, 1968, S. 169).

In addition, used in the composition the composition of the phosphors little compatible with the polymer base, whereupon the resulting material is low, has a low strength and a low extinction coefficients, which leads to inefficient conversion of sunlight.

It should also be noted that the kinetics of the luminescence of phosphors known in the composition is in the range 1 to 102ISS and does not coincide with the majority of the time characteristics of the Z-scheme of electron transport in the light stage of photosynthesis, which, as a rule, are of picoseconds, nanoseconds, seconds intervals, and this in turn also leads to less efficient use of HEADLIGHTS (ORT D, Govindjee, Whitmarsh j. and other Photosynthesis. M.: Mir, t .1, 1987, S. 728).

However, using a well-known compositions as phosphors only compounds europium does not cover the entire area of the HEADLIGHT, which reduces the efficiency of electron transport in the light-dependent photosystems.

Closest to the claimed is a composition comprising a compound of europium, selected from the group consisting of salts of organic and inorganic acids, one or more compounds from the class-diketones and the connection of class bidentate nitrogenous heterocycles or one of a number of trialkyl(aryl)farinaccio (C.RF 96119574/04, publ. 27.11.98).

However, these compositions are not effective enough, as used to generate compounds of europium have lifetimes of luminescence in the range of 300 - 700 MS and only cover a small part of the temporary stages of photosynthesis, which reduces the utilization of solar energy.

The technical task of the invention is to intensify the differential effects of light on various stages photoregulatory process by creating svenni course those stages photoregulatory process, are sent to the effect.

This object is achieved by way of influencing the process photoregulatory activity of biological objects luminous flux certain (necessary) intensity and impulseto, in which the luminous flux generated by the light passing through the material containing phosphors having lifetimes of luminescence in the range of 10-12- 10 S.

The task is also solved by a composition for implementing the method, consisting of a support and a phosphor selected from the group of compounds of rare earth elements, antimony, tin, manganese, boron, organic phosphors, oxides with lifetimes of luminescence 10-12- 10 effective amount of 0.001-10 wt.%.

The proposed solution produces photobiological low intensity (less than 1 mW/cm2pulse conversion of visible light in a wide wavelength range, as well as to increase the coefficient of digestibility" of visible light by taking into account the pulsed nature photoregulatory processes.

The method is as follows. Put the selected biological object (a plant, a cell, a microorganism, an animal or a person) in place peristiani light flux on the impact and conduct the irradiation of the biological object within the required time. When this time is determined empirically. Material containing phosphors and creates the specified luminous flux, is a polymer (high density polyethylene - HDPE, polystyrene, polyvinyl chloride - PVC, polymethylmethacrylate - pmmc, polypropylene, polycarbonate and others), glass, ceramics, etc. and can be made in the form of foils, plates, tiles, etc.

As the luminescent compound to create the light beam with the desired characteristics using known organic or inorganic compounds with lifetimes of luminescence from 10-210 C. these compounds include compounds of europium and/or terbium, or dysprosium, and/or samarium, and/or nitima with chelat forming compounds and some compounds of antimony, tin, boron, manganese, organic compounds, oxides. Changing the structure of the complexes, select the phosphor, which gives not only the required set of frequencies of the incident light, but also creates the necessary kinetics of luminescence of the phosphor.

As complex compounds REE use of the compounds of the composition Lnx(L)3yDn2Oh, where Ln Is Eu3+, Tb3+Sm3+Nd3+, (x=3-4, y=l,2, n=0-4), L - anions organically is/SUB>), terephthalic (TFt), acrylic and its derivatives, acetic acid (AC) and its derivatives of type - triperoxonane (TN), trichloro-, diphenylalanine acid, Caproic (CAP) and its derivatives, naphthoic acid (NF), salicylic acid, fatty acids aliphatic series, quinoline-carboxylic acid, sorbic acid, arylpyrazole, polycarboxylic acid, sulfonic acid, amino acid and so on; and-diketones include acetylacetone (AA), benzoylacetone (BA), dibenzoylmethane (DBM), triflluoroacetylacetone (TFAA), benzoyltrifluoroacetone (BTFA), hexafluoroacetylacetone (GFAA), thenoyltrifluoroacetone (TTA), phenylbenzimidazole (PMBP), 2-azetidinone (2-AD).

D-nitrogen or oxygen-containing neutral ligands: 1,10-phenanthroline (Phen), water (H2O), dimethyl sulfoxide (DMSO), Vexillology (DGSA), pyridine (P), 2,2-dipyridyl (DP), trioctylphosphine (TOPO), triphenylphosphine (TFO), tributylphosphine (TBPO), triethylamine (GAA), diethylamine (deja), diphenylguanidine (DFGA) and others.

As organic compounds can be used, for example, some organic acid (Anthranilic, naphthoic acids and their derivatives, derivative of naphthalimide, diimide perylenetetracarboxylic acid, livremente life of luminescence from a few to several tens of seconds and y in HEADLIGHTS.

Described compounds of europium lumines cent with wavelength =612 nm, similar compounds of terbium with lumines cent =550 nm, a Sm3+and Dy3+when =630-660 nm, 560-580 nm, respectively, and Nd3+when =800-1100 nm.

To influence photoregulatory processes in the region of 400 - 550, 660 and 800 nm with the necessary kinetics of the emission of use not previously used as phosphors compounds of tin, boron, antimony, manganese General formula Q+3[SbHal6]-where Q is primary, secondary and tertiary derivatives of amines; Hal is chlorine, bromine, iodine, etc. They can be entered into the composition, both independently and in a mixture with REE-containing complexes, organic phosphors and/or oxides. While their relationship when sharing is chosen empirically, based on the objective to obtain an intense luminescence in the desired wavelength range and photostationary system with a high extinction coefficient.

The above compounds phosphors are crystalline powder with a melting point above 160oC, soluble in most polar and non-polar solvents.

As the base composition may contain TNA the basis of the claimed composition are molar extinction coefficients =104-105in the area of the HEADLIGHTS (300 - 700 nm) and the power density of the light flux of less than 1 mW/cm2.

The introduction of luminescense compositions of the above compounds leads to the creation of the material, which improves the conversion of visible light in a wide spectral range of wavelengths, to increase its photostationary luminescence, as well as by varying the composition, to influence, for example, at certain stages of growth and development of plants. In addition, full or partial replacement of complex compounds of europium compounds of elements such as boron, tin, antimony, manganese leads to the reduction of the claimed composition.

The optimal concentration of the luminescent compound in the inventive composition, determined empirically, is 0.001 - 10 wt. %. Going beyond the claimed range leads to deterioration of physical and mechanical properties of the material, and in addition, at high concentrations occurs concentration quenching of luminescence, while smaller - not achieved the necessary luminescence intensity.

For sensitization of the intensity of luminescence of rare earth ions in the composition is injected compounds of dipoledipole experimentally.

For photostabilization received material composition may contain agents that are selected from a number of tinuviel, diazeam 5 pamasol-P, chimassorb-944 and others, and as plasticizers - wax, metallic stearates and other, already used for this purpose.

Use to create a light flux with the desired impulse response of the material defined luminescense composition results in low intensity (less than 1 mW/cm2) radiation with a given kinetics of luminescence, while the impulse response and the composition of the light flux impinging on photoregulatory process depend on the process in need of correction, and are created by the selection of such a composition that would provide the necessary characteristics.

For example, a composition comprising a Unit(KOR)33H2O+F2(BA) creates a luminous flux in the spectral ranges 400-470; 610-630 nm and lifetimes of luminescence equal to 400 μs and 1 NS, which intensifies the processes of photosynthesis of chlorophyll a, b-carotene, luteola.

Use in the claimed method luminescent compositions with different lifetimes of luminescence, allows us to provide the aqueous cycle plants depending on the type and stage of development of the necessary luminous flux with certain characteristics.

For example, to enhance the active absorption of auxiliary pigments of carotinoids in the region of 450-500 nm using a composition containing ANT + Sm(Bq)33H2O.

Comparative analysis of the proposed composition with the prototype shows that the claimed compositions compositions differ from the known introduction to the composition of antimony compounds, tin, boron, manganese, possessing kinetics of luminescence from 100 NS to 1 µs and allows you to create a material with a coefficient of conversion of visible light in a wide spectral range of wavelengths, to increase photostationary luminescence, as well as to create favorable conditions for certain stages of growth and development of plants.

Based on the proposed composition can be manufactured, for example, polymer films for growing plants in the greenhouse. For this purpose, the pre-polymers are thoroughly mixed with the appropriate amount of the claimed composition and then get the film one of the known methods, for example by extrusion or calandrinia.

The resulting polymeric materials physical and mechanical properties meet the requirements documentation. So, for a film based on polyethylene strength of linnie at break in the longitudinal direction to 250% in the transverse - 300%, which meets GOST 10354-82.

Photostationary polymeric material derived from the declared composition, 1.2 - 1.8 times higher than photostationary polymeric materials, activated only europicturesque the phosphors and more photostationary materials, activated inorganic phosphors.

To study photostationary phosphors samples of polymeric materials were subjected to accelerated laboratory testing in particularly harsh conditions of irradiation of a mercury lamp CES-250 for 5-15 hours, which corresponds to 120-360 days finding film under natural solar irradiation. Photostationary defined as the integrated intensity of luminescence in relative units after 10 h of irradiation. The intensity of luminescence was measured on device "NLL-1". The transparency of the films in the visible region spectrophotometer M-40.

Example 1.

A portion of the polymer composition 100.0 g, containing 0.1 g luminescense complex compounds UOM(NO3)32Phen and 0.1 g TV(NO3)3TFO (ratio 1: 1) and is 99.8 g of LDPE thoroughly mixed, and then pressed in a laboratory press at a temperature of 140is there film in the visible region of the spectrum. The resulting sample is irradiated under the lamp the TDR 250 for 10 hours and measure the residual intensity of the luminescence. The original intensity of the luminescence of the film, activated only connection europium, before irradiation take 1 at a concentration of activated additive is equal to the total concentration of both compounds.

Characteristics of the obtained film

The original intensity of the luminescence - 1,2

The intensity of the luminescence after 10 h of irradiation - 0,35

The extinction coefficient () - 1,0

Film characteristics, Eu-activated(NO3)32Phen

The original intensity of the luminescence - 1,0

The intensity of the luminescence after 10 h of irradiation - 0,18

The extinction coefficient () - 0,95

The table below shows examples of the materials on the basis of the claimed composition, prepared as described in example 1. Shows the spectral ranges luminescence intensity after 10 h of irradiation (fotostudio), extinction coefficients, the lifetimes of luminescence and specified stages of photosynthesis are affected by the following structure.

Thus, the proposed method of influence on the process photoregulatory activities.it is diversified conditions of life the light-induced biological objects, because they allow to create the desired spectral composition, impulseto and power, synchronous primary photoprocesses in biological objects.

1. The way to influence photoregulatory the process of biological objects by their irradiation luminous flux of a particular impulseto, characterized in that the luminous flux generated by the light passing through the material containing phosphors having lifetimes of luminescence in the range of 10-12- 10 S.

2. The method according to p. 1, characterized in that as phosphors use of compounds selected from the group of compounds of rare earth elements, antimony, tin, manganese, boron, and organic compounds and oxides.

3. Composition for luminescing material containing the base and the phosphors, characterized in that as phosphors it contains compounds selected from the group of compounds of rare earth elements, antimony, tin, manganese, boron, and organic compounds and oxides with lifetimes of luminescence 10-12- 10 effective amount of 0.001-10 wt. %.

4. The composition according to p. 3, characterized in that it additionally contains compounds of elements selected from the GRU is notice on PP. 3 and 4, characterized in that as the Foundation it contains a polymer selected from the group of thermoplastic or thermosetting polymers.

6. Composition according to one of paragraphs. 3-5, characterized in that it further comprises photostabilization selected from the group: tinuvin, diazeam 5 pamasol-P, chimassorb-944.

7. Composition according to one of paragraphs. 3-6, characterized in that it further comprises a plasticizer selected from the group of paraffin, metallic stearates.

 

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