The way to increase the fertilizing ability of sperm

 

(57) Abstract:

The invention relates to animal husbandry. The method consists of using a synthetic analogue of prostaglandin F2astropan - cloprostenol sodium salt as a biologically active substance, which is used at a concentration of 200 μg per 100 ml of diluted semen. The method allows to increase the fertility and prolificacy of sows during artificial insemination. 3 table.

The invention relates to livestock and can be used in the artificial insemination of pigs. For artificial insemination of sows use native, chilled or frozen semen of boars. In the latter two cases, it is diluted with protective environments in 2-3 times. Therefore, the content of biologically active components (for example, prostaglandins that regulate reproductive processes in females) in one spermatoza (100 ml) is 5-10 times less than in the boar ejaculate.

In this regard, the lower fertility of sows during artificial insemination diluted sperm in comparison with natural grass due to low content of prostaglandins in one sperm is army boars used in the artificial insemination of sows. For example, aminophylline, the substance is permitted by the Ministry of health for use in medicine as an antispasmodic remedy [1].

Aminophylline is administered in glucose-Gelato-citrate-sulfate (GHCS) environment with semen for 20-30 min Before insemination dose of 0,024 g per 100 ml of the diluted sperm.

However, this drug is used in medical practice as a vasodilator agent, cannot contribute to the full realization of the reproductive system of females, so as not involved in the endocrine regulation of reproduction cycles, i.e., does not stimulate the reproductive function of females.

The aim of the invention is to increase the efficiency of the method.

The objective is achieved by increasing the duration of the biological usefulness of the sperm of boars outside the body, improve the conception rate and multiple pregnancy of sows during artificial insemination.

The essence of the method consists of using a synthetic analogue of prostaglandin F2astropan - cloprostenol sodium salt [2], affect luteolysis yellow bodies, follicular growth and ovulation in females in CONV - glucose-chelating citrate-sulfate medium is prepared according to GOST [3]. Immediately prior to insemination of sows on Wednesday introduced estrofan rate of 200 mcg per spermatoza.

To select the optimal concentration of astropan increasing the fertility and prolificacy of sows experiment was carried out in the pig "mine" the Lipetsk region.

Example 1. Sperm was obtained from adult boars of large white breed by artificial vagina. After the capture of the sperm was determined by the volume of each ejaculate and the number of quality indicators (activity points, concentration in million/ml, the total number of sperm in the ejaculate in billion). Semen dilution was carried out according to the instructions in 2-4 times [3].

Research the sperm was taken to meet the requirements of the regulations (table.1).

Diluted semen was divided into 5 equal parts (100 ml). With one of the doses of each ejaculator was controlling it was administered aminophylline on 0,024 g per 100 ml of medium. Other groups (2-5) were experienced. Immediately before use they were introduced estrofan: 100 mg; 150, 200 and 250 μg/dose.

The absolute survival of spermatozoa (Sa) was determined after 24, 48, 72 h storage predelli the best survival rate (table.2).

The activity indicator control and experimental samples of spermatozoa differed slightly to 7.0-7.1 grade.

However, when you add in spermatozo 200 µg astropan absolute survival rate exceeded the control 40.E.

Example 2. For a comparative study of fertility and multiple pregnancy sows are inseminated offer a diluent, in the above mentioned doses of astropan and prototype - aminophylline was supplied to the experience of 185 sows, of which the control group was inseminated 35 heads (PL.3). In the production experiment, the influence of astropan at a dose of 250 mcg on the fertility of sows were not studied, because at this dose sperm quality was inferior to the control.

The obtained data (table.3) show that the fertility of sows in the 4th experimental group, which used the optimal dose of astropan (200 μg/dose) was higher than in the control group, 11.7%, and Farrow - 1.3 head (9.5 Piglet per litter).

Thus, the introduction into the environment of astropan at a concentration of 200 μg/dose immediately before artificial insemination of sows can significantly improve the biological integrity of sperm (absolute indicator vyzyvaemogo with control (medium with aminophylline 0,024 g/dose).

Sources of information

1. Means for increasing the fertilizing ability of sperm. A. S. N 1632495. BI N 9, 1991.

2. Guidance on the application of astropan. Led by spoof, United pharmaceutical factories, Prague, 1984.

3. Wednesday Lycosa-chelating citrate-sulfate for semen of boars. GOST-17637-72. M, Goskomstandart of the USSR, 1972.

The way to increase the fertilizing ability of sperm, including the introduction into the environment of biologically active substances (BAS), characterized in that as BAS use a synthetic analogue of prostaglandin F2-the sodium salt of cloprostenol at a concentration of 200 μg per 100 ml of diluted semen.

 

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