The method of obtaining the application tools

 

(57) Abstract:

The invention relates to pharmaceutical industry. Mix a solution of foaming agent (collagen of the swimming bladders of sturgeon and catfish), aqueous-alcoholic extracts, glycerin, dimethyl sulfoxide and water in the following ratio, wt. % of: water-alcohol extract of the leaves of eucalyptus or propolis, or kidneys various species of poplar, or purple Echinacea herb, or fruit, milk Thistle, or lavender flowers Kolosova, or leaves of Salvia officinalis, or buds of Betula pendula, or calendula flowers, or Melissa officinalis herbs, or roots of licorice, or bark of the common lilac, or rhizomes of Rhodiola rosea, or herb wormwood estragon (tarragon), or herb of monarda fistular - 7,8-39,0; collagen fish - 3,9-39,06; glycerin medical - 0,39-3,52; dimethyl sulfoxide - of € 0.195-0,98; purified water up to 100.0. The invention allows to obtain a product with more pronounced adhesive, antibacterial and regenerative properties. 3 table.

The invention relates to chemical-pharmaceutical industry, in particular the production of application of medicines in the form of phytoplankton body, and immune disorders.

In pharmaceutical technology a method of obtaining medicinal phytoplank [8] of the individual drug components and vegetable substances [9, 12, 13] , however, their production is carried out using as a film methylcellulose, sodium carboxymethylcellulose, gelatin, sodium alginate, agar-agar. This range of binders cannot be considered sufficient, given the high demand practical medical application in medicines. This prevents the expansion of the range of drug substances introduced into the composition of phytoplank.

A prototype of the invention taken us a way to get phytoplank drug, including the use of gelatin and methylcellulose (film), glycerol (plasticizer), polyvinylpyrrolidone (PVP) (osmotic agent), dimethyl sulfoxide (DMSO) (penetrator) and aqueous alcoholic extraction of medicinal plants.

This tool contains as the film-forming gelatin and methylcellulose, but not contains fish collagen, which provides greater adhesion force, electionaccording film-forming agents of natural origin for the production of application of medicines prolonged action.

This goal is achieved by the fact that as the basis of the dosage form using the collagen of fish obtained in the Caspian research Institute of fisheries (, Astrakhan) of the swimming bladders of sturgeon and large catfish. As an aid use glycerol (plasticizer) and DMSO (penetrator), and as a medicinal substances - water-alcohol extract of the leaves of eucalyptus, or propolis, or kidneys various species of poplar, or purple Echinacea herb, or fruit, milk Thistle, or lavender flowers Kolosova, or leaves of Salvia officinalis, or buds of Betula pendula, or calendula flowers, or Melissa officinalis herbs, or roots of licorice, or bark of the common lilac, or rhizomes of Rhodiola rosea, or herb wormwood estragon (tarragon), or herb of monarda fistular. When this separately prepared aqueous solution of the foaming agent by heating on a water bath. To the resulting solution was added plasticizer, the penetrator, mix, and add water-alcohol extract, and again mix. The resulting solution was poured into molds and dried at room temperature to a residual moisture content of 5%.

Conducted by the applicant additional search of the known technical solutions [1-8, 10, 11, 14-18] to detect in them the characteristics similar to the characteristics of the proposed method of obtaining, showed that these signs are absent, therefore, the claimed technical solution meets the criterion of "inventive step".

The inventive method is illustrated by the following example.

Example 1. In a mixer with a steam jacket (or water bath) place of 7.8 g (wt. %) fish collagen, add purified water 73,8 g and heated at the temperature of the water bath (60o(C) to dissolve the collagen. To the resulting clear solution was added 2.0 g of glycerol, 0.4 g of DMSO and stirred for low-speed mixer, and then add a water-alcohol extract (16.0 g) of medicinal plant materials: the leaves of eucalyptus, or propolis, or kidneys various species of poplar, or purple Echinacea herb, or fruit, milk Thistle, or lavender flowers Kolosova, or leaves of Salvia officinalis, or buds of Betula pendula, or calendula flowers, or herbs Melissa officinalis, or is Arjun), or herb of monarda fistular, and again mix. The prepared solution is poured into molds, pre-fat alcohol or coated with a hydrophobic layer. Dried at room temperature to a residual moisture content of 5%. After drying receive the application of the dosage form in the form of sticky and transparencies with the taste and smell weak source of vegetable raw materials.

Forms for the pouring of the films selected depending on their subsequent applications:

on the mucous membrane of the mouth - area of 2 cm2;

- for use in gynecology and dermatology - 4 cm2;

or pour out in the form of the total area of 553 cm2and then dosed according to the specified size.

Examples of other optimal proportions given in table. 1 (compounds 1-9). Manufacturing technology the application of the dosage form in the form of sticky phytoplank is carried out analogously to example 1.

Change quantities (beyond the parameters) of fish collagen and auxiliary substances (glycerol, DMSO) in the direction of decreasing or increasing (beyond the parameters) in relation to the listed ratios does not achieve a positive effect: the application and crumble, have an unpleasant taste.

To study the characteristics of the application dosage forms collagen-based fish taken two dosage forms containing an alcohol extract of poplar buds, propolis. Used microbiological method for the determination of antimicrobial activity of medicinal forms. As Comparators used phyto membrane-based methyl cellulose and gelatin, alginates (magnesium, potassium, sodium), polyvinyl alcohol, and collagen derived from cartilage of cattle in the meadows, in the form of 2% solution in acetic acid).

To conduct the study from each sample dosage forms carved in 5 films with a diameter of 1.3 mm and placed them on the surface of the nutrient medium. Previously on the surface of this nutrient medium by the method of the Lawn was planted reference culture of Staphylococcus aureus strain R-109. The disks studied phytoplank placed at equal distances over the entire area of the Petri dish. Crops were placed for one day in thermostat, and then measured the zone of growth inhibition in mm experiment was repeated three times. The results of microbiological tests are presented in table. 2.

So ootu compared to the rest of the basics.

To study the adhesive properties of the application medicines used a gravimetric method. Used scales manual, balanced Cup for weights and glass plate size 3,511 see was Determined by the total weight of the weights, causing loosening glass plates from medicinal application tools.

The analyzed samples of the films were cut exactly the same diameter (d= 1.3 cm), glued onto the glass surface, covered by a plate connected to the rocker weights, and pressed her cargo weight 100 g for 10 sec. Cup weights were loaded with weights to otkleivanie plate. For more accurate determination of the mass of the weights, causing loosening of the plate, the last mass was gained fractionally. 5 parallel measurements was calculated the average mass and determine the adhesion force according to the formula

F= mg,

where F is the force of adhesion, H;

G is 9.8 N/kg the acceleration of free fall.

The results obtained are presented in table. 3.

Thus, when comparing the results revealed the highest stickiness application for funds on the basis of fish collagen.

To study the possible interaction between the base (PL is in the thin layer of sorbent (TLC) plates "Silufol UV 254". As the mobile phase used solvent system: chloroform-ethanol in a ratio of 9: 1 and chloroform-methanol-water in a ratio of 26: 14: 3. The chromatogram showed in UV-light at a length of 254 nm and 366 nm. The analysis of the obtained chromatogram revealed all the major spots of biologically active compounds of alcoholic extracts with the corresponding values of Rfnot detected for more (foreign) spots.

Thus, film-forming agent fish collagen is the optimal basis for the manufacture on the basis of application of medicines in the form of sticky phytoplank.

Thus, using the proposed method of obtaining the application of the medicinal product provides the following benefits:

1. Application drug, obtained by the claimed method, has a more pronounced adhesive, antibacterial and regenerating properties compared with the prototype and known analogues.

2. The use of film-forming means fish collagen allows to expand the range of drug substances introduced into the composition of phytoplank, which creates a good basis for expanding the range lasalleman the way film-forming means fish collagen can produce drug phyto membrane as in an industrial environment based pharmaceutical factories and other pharmaceutical companies, and in pharmaceutical production.

Sources of information

1. Zapesochnaya gg , Kurkin Century A. Glycosides cinnamic alcohol from rhizomes of Rhodiola rosea // Chemistry of natural. connect. - 1982. - 6. - S. 723-727.

2. Zapesochnaya gg , Kurkin C. A. , Boyko VP , Kolher C. K. Containing promising natural biologically active substances of medicinal plants // Chem. - farmac. log. - 1995. , 29, 4. - S. 47-50.

3. Kurkin C. A. , Zapesochnaya gg , E. Avdeeva Century , Sentsov M. F. , fungal I. B. , Braslav Century B. , Pervushkin, S. C. , Tsybulko S. C. Quantitative determination silybin and amount flavolignans in the fruits of Silybum marianum (L. ) Gaertn. // Plant resources. - 1996. - T. 32, vol. 3. - S. 80-86.

4. Kurkin C. A. , Lebedev, A. A. , E. Avdeeva Century , Zapesochnaya gg , Pervushkin, S. C. , Simerini L. C. , Bulatova M. C. a Method of obtaining the extract, milk Thistle. - The patent of the Russian Federation 2102999. - A 61 K 35/78, A 61 K 9/08. - 27.01.98,, bull. 3.

5. Kurkin Century A. Containing promising natural biologically active compounds. - Samara. - 1996. - 80 S.

6. Kurkin C. A. , Avdeev O. I. , E. Avdeeva Century , Mizin, P. Quantitative determination of the amount hydroxycitric acids in aerial parts of Echinacea purpurea (L. ) Moench. //Plant resources. - 1998. , 34, in Oneida aerial parts of Artemisia dracunculus L. cultivated in the Samara region // Plant resources. - 1996. - T. 32, vol. 1-2. - S. 88-92.

8. Mashkovsky M. D. Drugs: In 2 volumes. - 14th ed. - M. : New Wave, 2000. - T. 1. - 539; So 2. - 608 S.

9. Mizin, P. , Kurkin C. A. , Kosarev centuries , Avdeeva, O. I. , E. Avdeeva Century , Braslav Century B. , Starostenko A. G. , Pravdivtseva O. that is, the Method of obtaining the medicinal phyto membrane // Patent RF 2155071. - A 61 K 47/34, 9/00 // (A 61 K 47/34, 47: 32, 47: 20, 47: 10, 35: 78), A 61 P 17/02, 37/00. - 27.08.2000,, bull. 24.

10. Muravyova D. A. Pharmacognosy. - M.: Medicine, 1991. - 560 C.

11. Muravyova D. A. tropical and sub-Tropical herbs. - M.: Medicine, 1983. - 336 S.

12. Oleshko L. N. , Oleshko, And. Means for treatment of the oral mucosa // Patent RF 2058783. - A 61 K 35/78. - 27.04.96,, bull. 12.

13. Oleshko L. N. Methodological aspects of creating phytoplank, suppositories, preventive and curative medicines on the basis of extracts of coniferous chlorophyllin sodium, propolis thick, Erakond". - Abstract. the dissertation on competition of a scientific degree of the doctor of pharmaceutical Sciences. - Perm, 2000. - 46 S.

14. Plants for us. Reference book / Ed. by G. P. Yakovlev, and K. F. Blinova. - S.-Petersburg: Academic book, 1996. - 653.

15. Register the drugs in Russia: a Handbook. - M. : Attraversare, 1997. - 1504 S.

17. Koch-Heitzmann I. , W. Schultze Melissa officinalis // z Phytotherapie. - 1991. - Vol. 11. N. 2. P. 50-58.

18. Leng-Peschlov E. , Strenge-Hesse A. Die Mariendistel (Silybum marianum) und Silymarin als Lebertherapeuticum // z Phytotherapie. - 1991. - Vol. 11, N. 2. - R. 50-58.

The method of obtaining the application of the medicinal product for the treatment of inflammatory diseases of the mucous membrane, skin and immune disorders, comprising mixing a solution of a film-forming, aqueous alcoholic extraction of medicinal raw materials, glycerin, medical dimethyl sulfoxide and purified water, filling in forms and drying, characterized in that the foaming agent used fish collagen from the swimming bladders of sturgeon and catfish and the mixing is carried out at the following ratio, wt. %:

Water-alcohol extract of the leaves of eucalyptus or propolis, or kidneys various species of poplar, or purple Echinacea herb, or fruit, milk Thistle, or lavender flowers Kolosova, or leaves of Salvia officinalis, or buds of Betula pendula, or calendula flowers, or Melissa officinalis herbs, or roots of licorice, or bark of the common lilac, or rhizomes of Rhodiola rosea, or herb wormwood estragon (tarragon), illsuited - of € 0.195 - 0,98

Purified water Up to 100.0

 

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