Ointment for healing burns and infected wounds

 

(57) Abstract:

The invention relates to medicine and applies ointment to heal burns and infected wounds of various etiologies, as well as for the prevention and formation of keloid scars. The invention lies in the fact that the ointment for healing burns and infected wounds comprises a proteolytic enzyme collagenase Clostridium histolyticum, as the antibiotic gentamicin sulfate, and as ointment bases the oxides mol.m. 400 and 1500 and glycerol at a ratio of components. The invention provides gentle, bloodless enzymatic cleansing wounds, stimulates the development of healthy granulation and does not preclude the epithelization process. 3 table.

The present invention relates to medicine and can be used for healing burns and infected wounds of various etiologies, and also to prevent the formation of keloid scars.

Known healing wounds (ed. mon. USSR N 1729513, 1992), containing aspersa, citric acid, sodium phosphate dosimeky, glycerin, propylene glycol, vaseline, emulsifier N 1, and water. The disadvantage of this tool is the absence in the mA is liskay to the present invention is an ointment (prospect company "Pliva", Zagreb, Yugoslavia, 1990) enzymatic cleansing wounds "Iruksol, including proteolytic enzyme collagenase, antibiotic ointment base and distilled water.

The disadvantage of it is that the lipophilic base of the drug has not dehydrating ability, which is especially important in the treatment of purulent wounds in the first phase of wound healing.

The technical result of the invention is to enhance antimicrobial and necroticism of drug action and the possibility of preventing the formation of keloid scars.

Closest to the present invention is an ointment for enzymatic cleansing wounds "Iruksol", including the enzyme clostridiales A (collagenase Clostridium histolyticum) and chloramphenicol on lipophilic basis when the component content in 1 g of ointment:

Clostridiales A - 0.6 U

Chloramphenicol 10 mg

Lipophilic base - rest

(prospectus of the company "Pliva", Zagreb, Yugoslavia).

The combination of active enzymes and broad-spectrum antibiotic in the drug Iruksol" makes it possible to cleanse the wound of necrotic masses, and prevents the spread of infection, but the lipophilic base of the drug does not have the ome, this ointment is not available for use in General practice, as is the import of patented tool (manufactured by a company "Pliva" in cooperation with the firm KNOLL AS).

The technical result of the invention consists in enhancing antimicrobial and necroticism of drug action and the possibility of preventing the formation of keloid scars.

The invention consists in that the ointment "Collegeteen" for healing burns and infected wounds contains a proteolytic enzyme collagenase Clostridium histolyticum, the antibiotic gentamicin sulfate, and as ointment bases polyethylene oxides (PEO) and glycerol in the following ratio, wt.%:

Collagenase - 160-200 ED

Gentamicin sulfate - 0,08-0,1

PEO-400 - 66,0-68,0

PEO-1500 - 16,5-17,0

Glycerin - of 10.0 to 10.1

Distilled water - rest

Collagenase Clostridium histolyticum is a proteolytic enzyme which is able to specifically cleave the molecule of collagen - the main component of connective tissue. This is based on a therapeutic and prophylactic effect of the drug is used for cleansing wounds of necrotic tissue, and also to prevent the formation of keloid scars. eat gentamicin, promotes rapid cleanse wounds of any etiology, as well as accelerate the process of granulation. Collagenase activity of the enzyme preparation was determined using the ninhydrin method and was expressed in units of leucine/mg of lyophilized. The activity of 1 mg of lyophilized amounted 0,20 - 0,25 UNITS (Ámol leucine) 20 min incubation in the reaction mixture.

Gentamicin sulfate is a broad - spectrum antibiotic, is one means of combating severe purulent infection, particularly caused by gram-negative flora, including Pseudomonas daddy.

Hydrophilic basis for this composition is also an active component. PEO-400 has a pronounced hydrophilicity caused by the formation of hydrogen bonds between macro - and macromolecule; improves the penetration of drugs into the depth of inflamed tissues, and dramatically enhances the activity of antibiotics and ensures maximum absorption of their ointments. PEO-400 is physiologically neutral connection, easy to apply on the wound surface and uniformly spreads all over them, does not interfere with gas exchange tissues, does not violate their physiological functions. xine and "give" them in the dressing providing a dehydrating effect. Due to the high hydrating ability of the hydrophilic base absorbs exudate, and with it, and microbial toxins, products of tissue destruction, dehydrates microbial cell, dramatically reduces its biological activity weakens virulent pathogenic properties of microbial pathogen. Pathogenic microflora, the original resistant to antimicrobial agents is becoming sensitive to them in the presence of PEO, resulting in substantially increases the antimicrobial activity of antibiotics, including gentamicin sulfate.

Glycerin, which is part of the ointment softens the drying action of the oxides.

The ointment was prepared as follows.

The oxides PEO-400 and PEO-1500 alloy at a temperature of +40oC, add the glycerin. The resulting mixture is cooled with stirring to 35-38oC. and Then lyophilized collagenase and the antibiotic gentamicin sulfate dissolved in distilled water and mixed with ointment base. The mixture is cooled under stirring to room temperature. 1 g ointment contains 1,6 - 2,0 ED collagenase activity. Ointment stored at a temperature of +4oC. Packed in tubes and the need to strengthen or speed up the action of the ointment used 2 times a day. Top dressings with warm saline solution.

Example 1. Get the ointment "Collegeteen" that contains components in the following amounts, wt.%:

Collagenase - 160 ED

Gentamicin sulfate - 0,08

PEO-400 - 66,0

PEO-1500 - 16,5

Glycerin - 10,0

Distilled water - rest

Example 2. Get the ointment "Collegeteen" that contains components in the following amounts, wt.%:

Collagenase - 200 U

Gentamicin sulfate 0.1 to

PEO-400 - 68,0

PEO-1500 - 17,0

Glycerin is a 10.1

Distilled water - rest

Experimental study of the effectiveness of our proposed ointment composition was performed on the model of local purulent and burn wounds in rabbits. The effectiveness of therapeutic action of ointment was evaluated by the dynamics of changes in the area of RAS, the timing of necrosis, granulation and epithelialization, temperature and body weight, total leukocyte count, and bacteriological, histological and cytological studies of biopsy specimens. Animals in the experimental group showed reduction of RAS in size, a significant reduction in terms of necrolysis and appearance of granules (PL. 1, 2, 3). It was found that the intensity lisaraye is pitalization bottom of the wound. In the control group of animals wounds for a long time remained covered by a thick scab and healing occurred significantly later than in the experimental group. Burn wounds experimental animals healed soft scar, excessive scarring was not observed. In addition, experiments were conducted on Guinea pigs to study the proposed funds for the regeneration of damaged tissue using the method of remotesite. It was found that the ointment "Collegeteen" significantly increases the fortress of postoperative scar on the 10th day, and therefore stimulates wound healing in the skin of Guinea pigs. When observing animals during the 30-day application of the ointment "Collegeteen" it was noted that the study ointment irritating and General resorptive action on the tissues of animals.

Preclinical study of ointment "Collegeteen" showed that she has multiple therapeutic effect due to the simultaneous bactericidal, necroticism, dehydrating and anti-inflammatory action. The use of materials leads to a gentle, bloodless enzymatic cleansing wounds, and is also a means of preventing the formation of keloid scars. Ointment stimulates granulation and processes of epitelial the and also in order to prevent the formation of keloid scars proposed tool is applied daily to the affected areas. The ointment can be applied after the reconstructive surgery that leads to complete resorption or flattening of the scar, less pain and itching in it.

Thus, we can conclude that the ointment "Collegeteen" is an effective tool for the treatment of purulent and burn wounds, as well as a means of prevention and treatment of keloids.

Ointment for healing burns and infected wounds containing a proteolytic enzyme collagenase, antibiotic ointment base and distilled water, characterized in that it contains antibiotic gentamicin sulfate, and as ointment bases polyethylene oxides (PEO) 400 and 1500 and glycerin in the following ratio, wt.%:

Collagenase - 160 - AD

Gentamicin sulfate - 0,08 - 0,1

PEO-400 - 66,0 - 68,0

PEO-1500 - 16,5 - 17,0

Glycerin - of 10.0 to 10.1

Water distillatory - Ostalnoe

 

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