The method of obtaining tympanoplasties transplant
(57) Abstract:The invention relates to medicine, namely to otorhinolaryngology and may find application in chronic suppurative otitis media with tympanoplasty. The method of obtaining tympanoplasties material is that used Vartanov jelly, fragments of which are placed on a nylon mesh, forming monoplast necessary area for 5 h carry out the exposure by ultraviolet rays of the spectrum. After 3 h from the start of exposure on the surface of this layer is applied 1 ml of 1% solution dioksidina, then the resulting film is removed with a nylon base. The resulting material is acellular collagen-elastic formation with polymerized amorphous matrix with elasticising properties, low immunogenic properties and optimal physical qualities anatomical and functional tissue replacement. 1 C.p. f-crystals. The invention relates to medicine, namely to otorhinolaryngology and may find application in chronic suppurative otitis media.Long-term clinical experience of the use of tympanoplasty different grafts (the wall of the vein, litestat, allosterically graft, scar membrane, polymeric materials) demonstrate a significant percentage of rejections(1, 2, 3, 5).The existence of a large number of different materials for tympanoplasty, as well as the ongoing search for new fabrics testifies to their imperfection and lack of decisive advantages of one or another of the graft in terms of his healing.Therefore, the development of new effective materials for tympanoplasty is the actual problem.Currently, the most common in clinical practice material for tympanoplasty is the fascia of the temporal muscle of the patient (autopaste), the application of which leads to the most optimal anatomical and functional results(6, 7, 9, 10).The advantages of autowaschanlage transplant include: the ability to create thin, easily drying, simulated and laid transplant. In addition, mesoderm its origin excludes the formation of secondary cholesteatoma, epidermal cysts. Elastic fibers that form various sizes and shapes, the network is well stretched, that attaches to the fascia elastischem (4).However, taking the fascia of the temporal muscle is additional operating trauma for the patient, sometimes with a number of complications (bleeding, purulent infection) and in the subsequent occurs the limit reuse of this material in case of relapse.Along with many positive properties autowaschanlage graft and good long-term results when it is used, there are reports of the possibility of necrosis in transplanted graft followed by the formation of the perforation, the development of granulation tissue and fibrous thickenings (4).The novelty of the proposed solution is that the authors first used the cords Vartanova jelly of the umbilical cord of the fetus mammals in order to obtain a graft for tympanoplasty.This object is achieved in that the material for transplant use film obtained by coating the strands Vartanova jelly of the umbilical cord of the fetus on a nylon mesh carry out the exposure for 5 hours by the rays of the ultraviolet spectrum. After three hours from the start of exposure on the surface of this layer is applied 1 ml of 1% solution dio the ski transplant.As the source material used tissue elements (Vartanov jelly) full-term umbilical cord fetal mammals.Within 20 hours after birth resetinput the site of the umbilical cord of the fetus, which is thoroughly washed with saline solution, wring out from blood clots from umbilical veins and arteries. After the longitudinal cutting of the umbilical cord and separation of vascular cells produce layerwise separation Vartanova jelly in the form of individual fibers (thickness up to 3 mm and a length of 40 mm), which are then placed on a nylon mesh so as to form monoplast rounded necessary surface area. For polymerization of the laid strands within 5 hours carry out the exposure by ultraviolet rays of the spectrum. To impart antibacterial qualities tympanoplasties material in three hours from the start of exposure on the surface of this layer is applied 1 ml of 1% solution dioksidina. In the end, forms a transparent film of a thickness of 0.5 mm with elastic properties.Moreover, we observed that the polymerization cords Vartanova jelly takes place only in case of availability of oxygen in the air from two sides (upper and lower surfaces of the graft)th microscopy of this material with the use of dyes was determined by the following picture: the absence of cells; the presence of intercellular substance, represented by three types of concentrically arranged fibers (elastic, reticular, collagen) and hydrophilic rich amorphous matrix.This material contains collagen matrix, which serves as the basis for the growing epithelium. In its composition does not include cellular elements, on the membrane surface which, as you know, are antigenic system (8), which generally excludes immunological response of transplant rejection.Thus, we can conclude that this material is acellular collagen-elastic formation of polymerized with an amorphous matrix having elasticity.The proposed method for tympanoplasties material allows you to create a graft for tympanoplasty with low immunogenic properties and optimal physical qualities anatomical and functional tissue replacement.Literature
1. Bystrany A. C. - Growing cicatricial membrane, replacing missing the eardrum. - //The Bulletin of torinla., 1986-N6-N-32-34.2. Zabirov R. A. and others - Results of surgical treatment of the sick who .18-19.3. Zabirov R. A. and others - Myringoplasty in patients with chronic mesotympanum. - In the book: Proceedings of the I Congress of torinla. Proc.Dokl.. Bishkek - 1999 - S. 41-42.4. Kulakov, L. A. Abstract.Kida.Diss... M, 1990.5. Ovchinnikov A. Y., Ball, I. A. - the Use of canned flap to form natinonally membranes in tympanoplasty. In the book: IX Congress of torinla. Proc.Dokl., Chisinau - 1988-N-286.6. Palacina O. K. - Surgical rehabilitation of patients with conductive hearing loss. In the book: VIII Congress of torinla. Proc.Dokl., M, - 1982 - N-21-23.7. Transfiguration N. A. Modern state and ways of improving microsurgery of the ear. in the book: VIII Congress of torinla. Proc.Dokl., M, - 1982 - S. 37-42.8. A. Rout fundamentals of immunology. M. Mir 1991, S. 55-57.9. Perrin, A. , R. Mendosa, Baril Ch. et.all. - Resultats anatomigues a long terme des homogreffes tympanigues. A propos de 170 cas. //Ann. Otolaryngol. chir. cervicovas. 1987-65-1-S. 26-28.10. Shenoi P. M. - Autogenous temporaus fascia grafts. //J. Laryng. - 1982-96-N4-S 801-810. 1. The method of obtaining tympanoplasties transplant, including the production of a material with elastic properties, characterized in that use fibers Vartanova jelly, fragments of which are placed on a nylon mesh, forming monoplast required area, t is I.2. The method according to p. 1, characterized in that after 3 h from the start of exposure on the surface of monoplast put 1 ml of a 5% solution dioksidina.
FIELD: medicine, in particular bioactive complex for organogenesis.
SUBSTANCE: claimed complex represents multicomponent, bulk, three-dimensional structure, containing human allogene mesenchyme and epithelial cells and at least one layer of biocompatible polymer in form of collagen structure on network matrix. Biological complex of present invention is useful in regenerative and reparative reconstruction of any biological structures of mesemchyme-epothelial or mesodermal and ectodermal origin. Also disclosed are unified method for rebuilding of three-dimensional tissue defects and effective method for treatment of various gullet, urinary bladder parries, skin, gorge, eardrum, kidney, etc. defects. Complex also may be used in production of donor tissue equivalent bank.
EFFECT: active complexes for organogenesis useful in medicine.
3 ex, 1 dwg, 24 cl
SUBSTANCE: method involves applying hydroxyapatite collagen material plate so that plate size is selected to be by 5-8 mm greater than trepanation opening.
EFFECT: enhanced effectiveness in making plastic repair of maxillary sinus; excluded allergic responses.
SUBSTANCE: method involves introducing sharp tip of hollow needle into bone cortical layer crack. The crack is filled through needle lumen by introducing Collapan gel mixed with radiopaque substance in minimum concentration providing radio contrast range excess above injured bone contrast. Soft tissues are pierced with the needle in the vicinity of crack filling area center. Needle bevel plane is set in crack plane projection and introduced into the crack. Then, needle tube is rotated with its bevel plane directed into crack space. The cerebral covering soft tissues are pressed to bone along the crack path above positioned needle tip projection and the mixture is introduced in the amount equal to crack cavity region under pressed sift tissue. The needle end is pulled and directed towards the opposite crack region without removing it from the soft tissues and the region is also filled. Collapan introduction into crack fissure is carried out under X-ray optoelectronic transducer control.
EFFECT: enhanced effectiveness of treatment; reduced risk of postoperative complications.
FIELD: medicine, ophthalmology.
SUBSTANCE: the present innovation deals with a drainage for treating glaucoma that includes collagen types II-III immobilized in polymer of acrylic and vinyl rows, includes a cytostatic covalently bound with collagen types II-III, hypotensive preparation and not less than one rigidity rib at certain ratio of components. The innovation provides improved ocular hydrodynamics, suppression of reparative and cicatricial processes around the drainage suggested.
EFFECT: higher efficiency.
6 dwg, 3 ex
SUBSTANCE: the present innovation deals with the method to accelerate mucosal healing due to the following technique: one should apply a membrane consisted of purified collagenic material obtained out of natural collagen-containing tissue onto the part of affected mucosa to provide the chance for mucosal reconstruction in this part and, also, it deals with mucosa-regenerating preparation and application of purified collagenic material obtained out of collagen-containing natural tissue for preparing mucosa-regenerating preparation. The innovation provides more modified method that accelerates mucosal regeneration, as a whole, and, particularly, after surgical operations associated with the plasty of oral fornix.
EFFECT: higher efficiency.
12 cl, 3 dwg, 5 ex
SUBSTANCE: bone-and-mineral product contains porous bone mineral particles produced from natural bone and having crystalline structure practically corresponding to natural bone structure and practically containing no endogenous organic material. The particles have fibers of physiologically compatible type II resorbable collagen at least on their surface. Mass proportion of type II collagen fibers and porous bone mineral is at least equal to approximately 1:40.
EFFECT: enhanced effectiveness in recovering combined injuries of cartilage and bone tissue in articulations having defects.
8 cl, 6 dwg
SUBSTANCE: collagen tubes can be used for connection and regeneration of damaged nerves. Tube is formed of single sheet of collagen-containing material. Sheet has smooth small-sized external barrier surface preventing connecting tissue from growing through it. Tube also has internal sift fibrous surface which helps nerve to grow at higher speed.
EFFECT: higher speed of regeneration of damaged nerves' prevention of scars.
18 cl, 4 att, 4 dwg
SUBSTANCE: method of biomedical material production based on titanium and cobalt alloy in mode CBC includes as follows. Exothermal mixture of raw reagents made of powdered titanium and cobalt is prepared, added with max. 4 wt % titanium hydride and max. 15 wt % amorphous nanodisperse powdered calcium hydroxyapatite (CHA) or nanocomposite calcium hydroxyapatite with biopolymers. This powdered raw mixture is pressed to preparation blanks placed to reactor CBC. Preparation blank is preheated to temperature 350-580°C. Combustion action is initiated in inert atmosphere with following release of end product. Produced biomedical material represents mushy alloy of formula TiCo of total porosity 55-70% at open porosity portion 90-98% with prolate pores sized 200-800 mcm. Pore partitions are also porous with prevailing round pores of diameter ca 100 microns. Surface of mushy alloy is coated with calcium, phosphor and oxygen compounds which are hydroxyapatite decomposition products with prevailing content of calcium perovskite.
EFFECT: material is noncytotoxic, cells are characteristically split and actively migrate inside of mushy space.
4 cl, 4 dwg, 3 ex
SUBSTANCE: bone of natural origin is cleaned, sawed up to 0.2-2.0 cm thick plates, washed with heated to 65°C 0.1 M pH 5.8-6.0 phosphate buffer, digested in 0.1-0.4% activated papain solution at 65°C during 24 hours, then washed in five volumes of water at 40-80°C, treated with 0.4 N alkali at room temperature during 10-24 hours, rinsed in running water, degreased in ethanol/chloroform mixtures in ratio 1:2 firstly, and 2:1 secondly, decalcified in 0.4-1 N hydrochloric acid, treated with 1.5-3% hydrogen peroxide during 4 hours, washed with purified water, then with ethanol, dried at room temperature, packed up and sterilised. Material for osteoplasty and tissue engineering represents compound, in which native collagen matrix space structure and natural bone mineral component are preserved, containing 25% collagen and 75% mineral matter. According to dry material analysis it includes less than 1% non-collagen proteins.
EFFECT: method improvement.
3 cl, 5 ex
SUBSTANCE: group of inventions refers to pharmaceutics and medicine and concerns method of producing bioabsorbable matrix containing native nonreconstructible collagen type I, same bioabsorbable matrix used for filling defected soft and hard tissues, as implant, also method of treatment, reparation or replacement of tissue with using the disclosed bioabsorbable matrix bioabsorbable matrix. Method of producing the bioabsorbable matrix includes after treatment of animal skin; herewith derma treated with aqua-alkali solution containing sodium hydroxide, potassium dihydrogen phosphate and aqueous or anhydrous borax at temperature 1 to 10°C; herewith derma treated with aqueous solution of sodium sulphate and sodium hydroxide; herewith derma treated with aqueous solution of sodium sulphate; herewith derma treated with aqueous solution of boric acid. At the third fourth and fifth stages the derma is treated with periodic agitation of the solution periodically cooled to temperature 1 to 10°C; and each stage two to five includes water flushing of derma to ensure neutral pH of rinsing water.
EFFECT: invention ensures production of bioabsorbable matrix containing native nonreconstructible collagen type I with collagen fibre structure identical to natural collagen-containing tissue, possesses considerable mechanical properties and is applied as a matrix for growth, directional regeneration, improvement of soft and hard tissue trophism and structures.
32 cl, 1 ex, 3 dwg
FIELD: medicine, in particular fluid composition useful as implant with controlled releasing of pharmaceutical agent.
SUBSTANCE: claimed composition contains biodegradable thermoplastic polyester essentially insoluble in aqueous medium or body fluid. Composition also contains biocompatible polar aprotic solvent which is compatible with aqueous medium or body fluid in blendability and dispersability. Fluid composition also contains leuprolide acetate. Method for production of fluid composition also is disclosed. Invention also relates to solid biodegradable implant forming in situ in patient body after injection of fluid composition to patient. Said implant allows dissipation of biocompatible polar aprotic solvent. Invention also relates to kit.
EFFECT: fluid resin compositions for leuprolide delivery of improved effectiveness providing decreased testosterone levels in body.
45 cl, 5 dwg, 7 tbl, 9 ex
FIELD: composite polymer biomedicine materials containing polymer binder, biocompatible filler and carbon reinforcing filler.
SUBSTANCE: claimed composition contains polymer binder, namely mixture of polymethylmethacrylate or methylmethacrylate copolymer with methylacrylate and monomer methylmethacrylate in ratio of polymer part to monomer from 1:0.3 to 1:0.5 mass pts (50-72 mass pts); peroxide initiator (0.05-0.5 mass pts): carbon continuous fibers of 200-1000 filaments made of hydratcellulose fiber of polyacrylonitrile fiber (2-10 mass pts); and hydroxyapatite as filler (25-40 mass pts). Method for production of material from claimed composition useful in manufacturing of jowl implants also is disclosed.
EFFECT: polymer material having natural bone-like properties.
3 cl, 10 ex, 1 tbl
FIELD: medicine, gel composition.
SUBSTANCE: claimed composition contains calcium sulfate and viscous biopolymers and can be easily introduced into defect part of damaged bone. Composition of present invention is capable of penetration into adjacent organs and is useful as physiologically acceptable filling material for bone concretion.
EFFECT: composition to prevent growth of undesired conjunctive tissues and to induce blood vessel growth and bone osteoblast development in earlier stage.
7 ex, 9 dwg, 1 tbl, 6 cl
FIELD: biomedical engineering.
SUBSTANCE: device has composite materials containing biologically active microparticles stimulating human bone tissue regeneration. Silicon, calcium and phosphorus particles combination is used in given carcasses as biologically active substance stimulating human osteoblast proliferation and differentiation and promoting osteogenesis and new bone calcification. Beside that, organic polymer is used in given carcasses as carrier having three-dimensional structure and external anatomical shape. It shows several properties compatible with bone regeneration and blood vessel neogenesis.
EFFECT: enhanced effectiveness of treatment; improved safety and cost-effectiveness.
27 cl, 6 dwg, 1 tbl
SUBSTANCE: material which is used in the form of a detail, executed by formation by moulding is described, and it represents the biocompatible binding, containing one or several bonds providing addition of calcium or phosphorus, differing that it is exposed to operations of superficial clearing intended for deducing on a surface. This material is preferably used for manufacturing of ventplants or osteal prostheses.
EFFECT: providion of availability on a surface added to binding calcium and phosphorus.
20 cl, 1 ex
SUBSTANCE: present invention pertains to compositions, with low coefficient of friction for use in friction units of living organisms. Proposal is given of an antifriction composition, containing, as polymer binding mixture (with ratio of components in the binder): methyl methacrylate monomer (27-36 mass units), polymethyl methacrylate polymer powder (72-64 mass units), initiator - benzoyl peroxide 1 mass units (polymer binder A); or a mixture (with ratio of components in the binder): methyl methacrylate (27-36 mass units), copolymer powder (72-64 mass units) with the following content of components: methyl methacrylate - 89 mass units, ethyl methacrylate - 8 mass units, methyl methacrylate - 2 mass units, as well as initiator-benzoyl peroxide - 0.6-1.5 mass units (polymer, binder B) and ultrahigh-molecular polyethylene with 1·106 - 12·106 dalton molecular weight, in powder form (2-15 mass units per 85-98 mass units of polymer binder). Compared to the prototype, the value of coefficient of friction decreases by 2-4 times.
EFFECT: design of a composition, which reduces friction in the maxillotemporal joint of a polymer implant.
3 cl, 4 tbl
SUBSTANCE: bioplastic material includes matrix base of fibrous structure which is a native form of hyaluronic acid and forms a flexible plate. The bioplastic material contains antimicrobial substances.
EFFECT: higher efficiency of wound healing.
2 cl, 1 tbl, 7 dwg, 2 ex
SUBSTANCE: in a nanostructured bioplastic material containing a native form of hyaluronic acid, a base is nanostructured hyaluronic acid produced by photochemical cross-link and having a cellular texture in the range of 50 to 100 nm.
EFFECT: higher clinical effectiveness of using the bioplastic material ensured by better adhesion and creating the optimal cell migration conditions in a wound.
1 tbl, 1 dwg