Method for the production of biologically active additive

 

(57) Abstract:

The invention relates to biotechnology and can be used in animal husbandry. The method involves the cultivation of microorganisms in a nutrient medium, the separation of the bacterial mass from the culture medium, mixing it with a protective environment and drying. To improve therapeutic and prophylactic efficacy in the composition of the microflora additives used bifidobacteria two types: acidophilus wand and propionate-acid bacteria. This fermentation is carried out pure cultures of Bifidobacterium longum T-89, Bifidobacterium thermophilum, TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P-1, which contribute in the amount of 8 - 10% by volume of the medium, and the ratio of cultures set equal to 2.5 : 2.5 to 1 : 4. In the result of simultaneous action of all the cultures of microorganisms significantly increased therapeutic and prophylactic properties of biologically active additives.

The invention relates to biotechnology and can be used in animal husbandry.

In industrial livestock prevention of gastrointestinal diseases of young animals caused by conditionally pathogenic and pathogenic microflora, should be aimed at increasing the level of estastistica with biologically active agents, containing symbiotic intestinal flora as bifidobacteria. Bifidobacteria as natural factors of nonspecific defense of an organism, it is advisable to enter in the diets of young animals during the whole growth period: to accelerate the colonization of bifidobacteria in the intestine immediately after birth, which helps protect newborns from conditionally pathogenic and pathogenic microorganisms, as well as restore and maintain the required level of bifidobacteria in dysbacterioses, resulting from stress and treatment with antibiotics and antibacterial drugs, to replace antibiotics in animal feeding.

To enhance therapeutic and prophylactic properties bifidobacteria drugs appropriate sharing of bifidobacteria with other members of the normal intestinal microflora.

Known bacterial preparations "Bifidumbacterin", "actonelbuy", "biphidumbacterin", "bitonal", "probyvat" and others, as well as biologically active additive and tools /1,2,3/ possessing curative properties, the composition of the microflora which is administered bifidobacteria. The technology of obtaining bacterial preparato the x nutrient media for the purpose of accumulation of biomass, the concentration of biomass, as well as its preservation, involving in most cases the use of drying in a variety of ways.

The closest in technical essence to the invention is a method of obtaining a bacterial preparation for feed products containing bifidobacteria and lactic acid bacteria, providing for the accumulation of biomass of bifidobacteria in conjunction with Lactobacillus acidophilus and Lactobacillus Streptococcus (U.S. Pat. 2035184 Russia, MKI6A 61 K 35/66, C 12 N 1/20) /4/.

The disadvantage of this method is the use in zakvaskoy microflora only cultures of bifidobacteria (one species) and lactic acid bacteria, which reduces the preventive properties of the drug.

The aim of the invention is to increase therapeutic and prophylactic efficacy of biologically active feed additives.

The invention consists in the combined use in the composition of the microflora of biologically active additive Bifidobacterium, Lactobacillus acidophilus and propionate acid bacteria. To this end the fermentation of a nutrient medium is proposed to carry out the following cultures: Bifidobacterium longum T-89, Bifidobacterium thermophilum received on the proposed method, due to the simultaneous action of all cultures used. Additional introduction in the composition of the microflora of biologically active additives obtained by the present technology, propionate acid bacteria, which are active producers of vitamins of group B (B12B2, Pantothenic and nicotinic acid), helps to increase therapeutic and prophylactic properties of the additive in comparison with known probiotic preparations.

Example. When implementing the proposed method uses strains of bacteria, which is the property of the Museum of pure cultures of microorganisms of the Department of epidemiology and Microbiology ugma them. N. In.Vereshchagin and maintained in working condition. Cultivation of bacteria is carried out in a nutrient medium of the following composition: lactose - 5%, peptone - 5%, whey - 10%, yeast extract - 5%, corn extract - 1%, iron sulfate 7 - water - 0,5%, gidrolizovannogo milk - 10%, water to 100%, a pH of 6.6. The nutrient medium is sterilized at 121oC for 15 minutes, then cooled to 37oC and fermented pure cultures of Bifidobacterium longum T-89, Bifidobacterium thermophilum, TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P-1 with the initial value crops to 2.5 : 2.5 to 1 : 4, Cotecna 20-24 hours to reach pH 4.6. At the end of the growth culture medium is cooled to 14oC and carry out the separation of the biomass from the culture medium by centrifugation. The resulting biomass is mixed with a protective medium and dried.

The proposed method of obtaining biologically active additive will allow you to:

to implement the integrated use of cultures of bifidobacteria, Lactobacillus acidophilus and propionate-acid bacteria;

to obtain 1 g of the additive content of viable cells of Bifidobacterium up to 1012, Lactobacillus acidophilus - up to 109, propionate acid bacteria - up to 109;

to use a dietary Supplement for prevention of gastrointestinal diseases of young farm animals or to apply for the enrichment of the feed means on the basis of raw milk.

Sources of information

1. Platonov A. C. Production of products for livestock on the basis of the symbiotic micro-organisms in the gastrointestinal tract. -M.: 1985, -44 C.

2. Grunska C. A., Ehrwalder T. M., Gudkov, S. A. the Use of bifidobacteria in the diets of young farm animals. -M.: Ahronheim. -1991. -24 C.

3. TU 10. 02.925-91. Substitute whole milk dry kislomolochnuyu nutrient medium, incorporate the culture of bifidobacteria and Lactobacillus acidophilus, the accumulation of biomass of microorganisms and separate it from the culture medium, mixing the biomass with a protective environment and drying, characterized in that, to improve health care properties, the composition of the leaven of the additionally injected culture propionate acid bacteria, fermentation environment carry out pure cultures of Bifidobacterium longum T-89, Bifidobacterium thermophilum, TP-8, Lactobacillus acidophilus 97 and Propionibacterium shermanii P-1 with the initial value crops 2,5:2,5:1:4 who contribute 8-10% the volume of medium and grown together at a temperature of 37-38C for 20-24 hours to achieve a pH of 4.6.

 

Same patents:

The invention relates to the microbiological industry and relates to a new strain used to obtain entomopathogenic drugs

The invention relates to medical Microbiology and comes to obtain strains of probiotics
The invention relates to medical Microbiology and represents the strain mikroskopische fungus Aspergillus fumigatus Frezenius 157/32

The invention relates to biotechnology and concerns bacteriocin from Micrococcus varians, a nucleotide fragment encoding the bacteriocins, strains of Micrococcus varians CNCM 1-1586 and CNCM 1-1587 producing the specified bacteriocins, as well as method of obtaining bacteriocin and its use to obtain food and cosmetics as an agent, active against pathogens

The invention relates to biotechnology and Microbiology, and can be used in veterinary medicine for the prevention and treatment of inflammatory disease and dyspepsia in animals

The invention relates to the field of Microbiology, relates to a new strain of Trichoderma sp

The invention relates to methods of water quality control

The invention relates to the field of biotechnology and medicine

The invention relates to the microbiological industry and relates to a new strain used to obtain entomopathogenic drugs

The invention relates to medical Microbiology and comes to obtain strains of probiotics
The invention relates to medical Microbiology and represents the strain mikroskopische fungus Aspergillus fumigatus Frezenius 157/32

The invention relates to biotechnology and concerns bacteriocin from Micrococcus varians, a nucleotide fragment encoding the bacteriocins, strains of Micrococcus varians CNCM 1-1586 and CNCM 1-1587 producing the specified bacteriocins, as well as method of obtaining bacteriocin and its use to obtain food and cosmetics as an agent, active against pathogens

The invention relates to biotechnology and Microbiology, and can be used in veterinary medicine for the prevention and treatment of inflammatory disease and dyspepsia in animals

The invention relates to the field of Microbiology, relates to a new strain of Trichoderma sp

The invention relates to methods of water quality control

The invention relates to the field of biotechnology and medicine

The invention relates to biotechnology, veterinary science, medicine and can be used for the prevention and treatment of infectious diseases of fish, animals and humans, caused by microorganisms
Up!