The strain of the fungus beauveria bassiana (bals) vuill bkm f-3732d used to obtain entomopathogenic drugs
(57) Abstract:The invention relates to the microbiological industry and relates to a new strain used to obtain entomopathogenic drugs. The strain of Beauveria bassiana (Bals) Vuill the resulting directional variability of natural microorganisms and deposited in the collection of microorganisms, Institute of biochemistry and physiology of microorganisms, Russian Academy of Sciences under VKM F-3732D. Has a high productivity in comparison with the known strain in terms of the depth of fermentation - 1,2-2,0109JV/ml When grown on solid nutrient medium productivity is 2.2-5,1109SP/, the Biological activity of the culture liquid strain when making food, as defined by the death of the larvae of the locust, is 72-92%, and at the soil - the destruction of egg pods locust is 69-92%. table 1. The invention relates to the microbiological industry, and can be used in the production of entomopathogenic products against pests of agricultural crops and forests.One way to control pests such as Colorado potato beetle, Codling moth, boyaryshnia, locusts and other,of the fungus Beauveria bassiana.A known strain of the fungus species Beauveria bassiana (A. S. N 1688820) is suitable for surface cultivation, which is less technological than deep cultivation.The aim of the invention is to obtain a strain of the entomopathogenic fungus, with increased productivity of viable conidia and able to conduct in-depth mode of fermentation.Strain the resulting directional variability in the productivity of the most active culture, isolated from natural sources in the field of mass mortality of insects in terms of the Siberian region.The strain of Beauveria bassiana deposited in the collection of microorganisms, Institute of biochemistry and physiology of microorganisms, Russian Academy of Sciences, Moscow, under the number VKM F-3732 D.The Beauveria bassiana strain BKM F-3732 D is characterized by the following cultural-morphological features.When grown on solid agar medium wort (1:3) at a temperature of 27+1oC for 10 days forms a colony rounded, opaque, conidial layer chalky consistency of white color, the pigment does not form.Morphological signs of strain:
During cultivation in liquid nutrient medium hyphae branched, segmentina the round 12-34oC (optimum 27+1oC).PH of 5.0-7.0 (optimal 5,0-5,6)
As a source of nitrogen use peptone, casein, fish bone meal, meat broth, yeast and their hydrolysates, nitrites, corn extract.The sources of carbon:
Metabolizes sucrose, glucose, lactose, maltose, fructose, rhamnose, arabinose, starch. Not absorbed cellulose. Starch hydrolyses.In relation to the oxygen - mushroom aerobe.Phages are not detected.The productivity of the strain on solid substrates (millet) to 5.1 billion JV/g substrate, liquid nutrient media to 2.0 billion SP in ml.Strain well kept on wort agar at a temperature of 4-6oC or in flasks on the millet at room temperature for 1.5-2 years.Example 1.In a liquid nutrient medium of the following composition, %:
Molasses - 7,0
Corn extract - 1,2
Sulphate of magnesium, 0.1 to
Potassium phosphate one-deputizing - 0,2
Vegetable oil - 0,5
pH - 5,6-6,2
make not less than 0.5% lively with millet seed and are fermented with stirring, a constant air flow of 0.4-0.8 l/l environment in minutes at a temperature of 26-2 Example 2.The nutrient medium of the following composition in %:
Molasses - 5,0
Corn extract - 1,0
Magnesium sulfate - 0,005
Potassium phosphate one-deputizing - 0,1
pH is 6.4 to 6.5
sowing with wheat grown seed within 24 hours and in the amount of 10-15% (inoculum) is served in a nutrient medium, similar to example 1, and carry out cultivation with constant stirring, the temperature of 26-28oWith the air flow of 0.8 l/l environment in min for 28-35 hours to obtain a grid with conidia.The obtained culture liquid (K. g) analyze productivity, biological activity and heat stability.The evaluation results presented in the table, are far superior in their properties known strain K-87 and will provide a more concentrated form of the entomopathogenic of the drug. The strain of Beauveria bassiana (Bals) Vuill VKM F-3732D used to obtain entomopathogenic drugs.
SUBSTANCE: the present innovation deals with immunization in plants, particularly, compositions and methods for inducing plant resistance to phytopathogenic organisms, such as phytopathogenic fungi. An agent indicated for inducing plant resistance to phytopathogenic microorganisms is being an extract out of biomass of non-phytopathogenic microorganisms. The method to obtain the above-mentioned agent includes the following stages: a) resuspending against 50-200 g (dry weight) biomass of non-phytopathogenic microorganisms in 1 l either inorganic or organic solvent, b) mixing at room temperature for 1-12 h, c) incubating, d) resuspending, e) cooling up to room temperature at maturing and f) filtrating, not obligatory. The innovation enables to induce plant resistance to phytopathogenic microorganisms.
EFFECT: higher efficiency of immunization.
12 cl, 6 ex, 5 tbl