The method of feeding the chickens and the method of producing drug for feeding chickens

 

(57) Abstract:

The method of feeding the chickens includes adding to the feed Association Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33 in the ratio of 1:1 with a permissible deviation of 20% with the concentration of microorganisms from 50 to 500 million cells per 1 ml of solution, and adding the drug in food producing at a rate of 0.1 g at day 1, the head of the chicken. The method of producing drug for feeding chickens is that uterine culture containing a consortium of bacteria ARRIAM 1-33, sow in the amount of 1-5% in a nutrient medium consisting of 5% sunflower or soybean meal and 95% water, which is pre-sterilized for 20 min at a pressure of 1 atmosphere, and pH of 6.8 to 7.0, and then cooled to a temperature of 35-40oWith, and then spend cultivation in flasks or fermenters with a capacity up to 1000 l for 1 day. The obtained bacterial production culture containing a consortium of bacteria 1-33, mixed with sunflower or soybean meal in the ratio of from 1: 1 to 1:2, then, passing dry air, dried at a temperature of 40-60oC to a residual moisture content 7-7,2% and then dried the product is crushed to particle size of not more than 2 mm, 2 S. and 2 C.p. f-crystals, 1 table.

Known and the closest to the claimed method according to the technical nature of the method of feeding the chickens, described in the ed. St. N 1398796, MKI A 23 K 1/165, C 12 N 1/00, published in the Official Bulletin of the inventions of the USSR N 20 from 30.05.88, in which to improve the digestibility of feed in it add a consortium of bacteria ARRIAM N 1-32, including Ruminococcus sp., Lactobacillus sp., Azomonas sp. (prototype).

This method provides a high gain of Chicks: so, when you add in feed preparation consortium in the amount of 0.1 g per head gain increase of gain in live weight of chickens by 25.7% and the digestibility of cellulose by 10.3% compared to control.

The claimed method for producing a product for feeding chickens closest in technical essence is a method of obtaining the production of culture consortium, deposited in the culture collection of microorganisms ARRIAM number 1-32 described in the above author. St. N 1398796. The consortium 1-32 isolated from the contents of cattle, consists of bacteria Ruminococcus sp. N 35, Lactobacillus sp. N 35, Asomonas sp. N 35.

According to this method, bacterial production culture Association derived from lactic culture Association, which is seeded in the SS are in bottles with a capacity of 5-10 litres or in fermenters with a capacity of 50 l on the environment Mellanskog.

The preparation obtained by the above-mentioned method, has no toxicity and pathogenic for animals.

The disadvantages of this method are the complexity of the cultivation of the consortium in connection with the complexity, complex medium composition for culturing, and extremely unstable relationships between the components of the consortium.

The task of the claimed group of inventions is an increase in live weight of chickens by obtaining feed additives, including bacteria synthesizing enzymes and improves the digestibility of the feed, with the possibility of their use in commercial poultry production.

This problem is solved due to the fact that in the method of feeding the Chicks add in feed preparation containing microorganisms, the microorganisms use the consortium (Association) bacteria ARRIAM N 1-33, including Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33.

The consortium of bacteria 1-33 includes cellulolytic bacterium Ruminococcus albus 1-33 and the lactic acid bacterium Lactobacillus acidophilus 1-33, both bacteria represented in the consortium in the ratio of 1:1 with a permissible deviation of 20%, i.e. from 1:0.8 to 1:1,2.

The consortium of bacteria is th cattle by treatment and long-term multi-selection on the basis of "high cellulose activity".

Its components are deposited in the collection of the all-Russian State research Institute for control, standardisation and certification of veterinary preparations, October 18, 1999 under registration numbers Ruminococcus albus 1-33-DEPT and Lactobacillus acidophilus 1-33-DEPT.

The consortium 1-33 has no toxicity and pathogenic for animals. Due to the fact that the consortium can multiply in the gastrointestinal tract of animals, provides a positive aftereffect in its application.

The inventive method of feeding with the addition of the feed consortium of bacteria 1-33 increases the gain in live weight of chickens compared to control.

In the particular case of the implementation of the proposed method of feeding chickens produced with the addition of the feed is obtained on the basis of the consortium 1-33 drug saturated with microorganisms in the number of 50-500 million cells per 1 ml of solution, provided that its addition will be made in the amount of 0.1 g at day 1, the head of a chicken.

The optimal concentration of the consortium in the preparation and the daily rate of its feeding chickens established empirically. Determined that chick initial period, i.e. from the moment of hatching, the chick in age from 28 to 42 days the amount of the drug should be 1% of the mass is fed to the feed. However, given the fact that in the final period of the grown chickens eat more quantity of food, it turns out that they consume the same quantity of drug per day per head as in the initial period. Thus, it appears that the daily amount of each chicken of the drug does not depend on the age and rate of 0.1 grams per day.

The inventive method of feeding increases the gain in live weight of chickens at 28% and the digestibility of cellulose by 11% compared with the control, which exceeds the results achieved when using the prototype method.

Implementation of the proposed method is carried out by means of drug - bacterial production of crops for feeding chickens, method of production which includes the following steps: uterine culture containing a consortium of bacteria seeded in a certain amount in a nutrient medium, which is pre-sterilized with 1 ATM. within 20 minutes, providing a pH of 6.8 to 7.0, cooled to a temperature of 35-40oC, and then carry out the cultivation in flasks or fermenters.

Distinctive features of the proposed method we shall receive from the mother culture of the consortium of bacteria 1-33, which is seeded in the amount of 1-5% in a nutrient medium consisting of 5% sunflower or soybean meal and 95% water, and the process of cultivation are in flasks or fermenters with a capacity of up to 1000 liters per day.

While uterine culture contains a consortium of bacteria ARRIAM N 1-33, including cellulolytic bacterium Ruminococcus albus 1-33 and the lactic acid bacterium Lactobacillus acidophilus 1-33, presented in a ratio of about 1:1.

When grown in laboratory conditions uterine culture consortium of bacteria 1-33 as carbon source using a filter paper. The consortium has 1-33 high cellulolytic activity, it decomposes filter paper for 3 days at 90%. Cellulolytic activity of this consortium is higher than the activity of the consortium 1-32.

The consortium of bacteria ARRIAM N 1-33 stored and maintained on the environment Imshenetskii of the following composition: meat-peptone broth 500 ml tap water 500 ml, CaCO32 g, the filter paper 75, Wednesday pre-sterilized for 20 minutes at 1 ATM. and pH of 6.8 to 7.0, and then cooled to a temperature of 35-40oC.

Thus, as a consortium of bacteria 1-33 and podsolnechnikovye or soybean meal and tap water), it contains only two biological component and the ratio between them is stable. However, as a result of use of this method the drug (bacterial culture), as well as the preparation obtained by the method prototype is obtained in liquid form.

It is known that any drug in the liquid state retains its quality not more than 2 weeks due to the difficulty hermetic sealing and falling into the drug extraneous microflora, including toxins. As a consequence, obtained in this way the drug can not long-term storage, which prevents its use in commercial poultry production.

In the particular case of the implementation of the inventive method of producing drug for feeding chickens obtained bacterial production culture containing a consortium of bacteria 1-33, mixed with sunflower or soybean meal in the ratio of from 1:1 to 1:2, then, passing dry air, the dried mixture at a temperature of 40-60oC to a residual moisture content 7-7,2%, and then dried the product is crushed to a particle size not larger than 2 mm

Thus obtained dry product is fed to the chickens.

To obtain the product in a dry form was when Apolo, while getting dry preparation with the use of other compounds, such as bran, hay, flour or animal feed, after drying in a mixture is maintained from 5 to 20% of bacteria, and when mixed with sunflower or soybean meal (due to the presence of oil residues) from 50 to 70% of bacteria.

It was also established experimentally that in a dry drug, derived from the consortium 1-32 mixed with sunflower or soybean meal, preservation of bacteria is 10-20%, which is much lower than in the drug-based consortium 1-33.

In addition, in this way we obtain a dry product with a simple and cheap nutritional environment on the cake.

All quantitative ratios obtained experimentally. The mixture of culture consortium 1-33 with sunflower or soybean meal, dried at a temperature of 40-60oC to a residual moisture content 7-7,2%, passing dry air, for example, an inner air-circulating dryer described in the textbook "Technology of medicinal forms", edited by L. A. Ivanova. - M.: Medicine, 1991, S. 74.

As a result of the drying process of the drug he formed "baked in" fractions of various sizes, including relatively large - unsuitable for STIs not more than 2 mm The milling process can be accomplished in any conventional manner, for example by means of the crusher-mill universal corporate governance-2, described in the Handbook "Feed. Preparation, storage, use" authors centuries Shcheglova and L. G boyar. - M.: Agropromizdat, 1990, S. 209.

The dry form of the drug received allows you to store it for more than 3 months.

Thus, the use of this particular case, the inventive method allows to obtain the drug for feeding chickens with a high degree of preservation of bacteria in a dry form suitable for industrial use in poultry.

The invention is illustrated by the following example:

Feeding chickens is carried out with the use of drug-based consortium of bacteria 1-33, including Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33, in the form of liquid culture and dry powder. Biological effectiveness of the consortium study on chickens 28 - day age. Parallel to test the influence of the drug, derived from the consortium 1-32 in the form of liquid culture (prototype). Control is the feeding of the chickens of the usual food without biological additives.

To this end, take 4 groups of chickens (growing chicken pot all groups are in the same conditions of feeding, care and maintenance. Each group of Chicks fed separately: the first (control) - ordinary food, the second (prototype) - with the addition of liquid culture 1-32), and the third with the addition of liquid culture consortium 1-33, but the fourth - with the addition to the feed of the dry drug-based consortium 1 - 33 is based on 0.1 g of any drug on the head of the chick in the day. After two weeks, the Chicks were weighed.

The results are given in the table.

From the data presented in the table shows that the claimed method of feeding the Chicks use as a biological additive drug-based consortium of bacteria 1-33 obtained also claimed process is more efficient compared to the prototype.

1. The method of feeding the chickens, introducing into the feed of the bacterial preparation, characterized in that as a bacterial drug use consortium of bacteria ARRIAM 1-33, including Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33 in the ratio of 1:1 and a possible deviation of 20%.

2. The method of feeding the chickens by p. 1, characterized in that the concentration of microorganisms in bacterial drug ranges from 50 to 500 million cells per 1 ml of solution, and adding the pre is that feeding chickens in the form of bacterial production, namely, that uterine culture containing a consortium of bacteria seeded in a certain amount in a nutrient medium and carry out cultivation in flasks or fermenters, and the nutrient medium is pre-sterilized for 20 min at a pressure of 1 ATM and a pH of 6.8 to 7.0, and then cooled to a temperature of 35-40C, characterized in that as a uterine culture using a consortium of bacteria ARRIAM 1-33, including Ruminococcus albus 1-33 and Lactobacillus acidophilus 1-33, which is seeded in the amount of 1-5% in a nutrient medium consisting of 5% sunflower or soybean meal and 95% water, and the culturing is carried out in flasks or fermenters with a capacity up to 1000 l for 1 day.

4. The method of producing drug for feeding chickens on p. 3, characterized in that kultivirovaniya bacterial production culture containing a consortium of bacteria 1-33, mixed with sunflower or soybean meal in the ratio of from 1:1 to 1:2, then, passing dry air, the resulting mixture is dried at a temperature of 40-60C. to a residual moisture content 7-7,2%, and then dried the product is crushed to a particle size not larger than 2 mm

 

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Feed additive // 2131678
The invention relates to poultry, in particular for feeding birds

FIELD: veterinary medicine.

SUBSTANCE: poultry's diet during the first and second phases of egg yield should be supplemented with roxazym enzymatic complex at the ratio towards husk-bearing components being 0.007 - 0.009 : 20. The present innovation prevents inflammation of oviducal vagina in laying hens.

EFFECT: higher efficiency of prophylaxis.

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