The method of secondary prevention of disorders of spermatogenesis when exposed to radiation

 

(57) Abstract:

The invention relates to medicine, in particular for secondary prevention post-radiation impaired spermatogenesis, and can be used in medical and sanatorium-and-Spa institutions. For this animal in the early post-radiation period daily for 21-24 days administered intragastrically at 3.0 - 3.5 ml of one of the following mineral water: sulfate sodium-magnesium-calcium concentration of sulfate 2.6 g/l mineralization 3.1 g/l or the same sulphate sodium-magnesium-calcium water, but with the addition of the trace element silicon with a concentration of 150 g/L. This leads to the fact that decreases the degree of destructive processes in the spermatogenic epithelium remains significantly large number of germ cells, including their most Mature form (Spermatid and sperm), activated adaptive processes, and the index of spermatogenesis remains at a higher level compared with the control.

The invention relates to medicine, in particular to methods of secondary prevention post-radiation impaired spermatogenesis, and can be used in medical and sanatorium-and-Spa institutions.

Known methods of treatment and prevention of radiation sickness drugs (menadione and others ), but they have side effects and their influence on the processes of spermatogenesis remain outstanding. Drugs that stimulate spermatogenesis (lutrelle, perganol and others) also obraduyutsya.

There are also known ways of preventing radiation defects by the use of drugs of biological origin that increase the radioresistance of the body and at the same time do not have severe side effects (phyto - and toproperty, multivitamins and other). For example, biotrin, active substances which are metabolic products of fungi strain PS-64 (proteins, carbohydrates, unsaturated fatty acids, macro - and micronutrients and other) used for the prevention of disorders of haematopoiesis when exposed to radiation (Reference Vidal, 1998). To prevent spermatogenesis after exposure to radiation such drugs us not detected.

The closest to the essence of the proposed method is a method of rehabilitation of radiation injuries (patent N 2093161), consisting in the use of potable mineral waters iododerma sodium chloride content of iodide-ions 14 mg/l and salinity of 4.1 g/l; Moscow sulphate sodium-magnesium-calcium concentration of sulfate 2.8 g/l and salinity of 3.5 g/l; silicon-containing sulphate-hydrocarbonate calcium-sodium concentration of silicon 139 mg/l and salinity of 7.3 g/l; boron chloride-hydrocarbonate is asfiles persistent and long-lasting damage in internal organs (state testes are not described).

The technical result of the proposed method is to prevent and limit the development of post violations in the spermatogenic epithelium of the testes and preservation processes of spermatogenesis at a higher level in the early post-radiation period.

This technical result is achieved by the fact that animals early after radiation exposure daily for 21-24 days administered intragastrically at 3.0-3.5 ml of one of the following mineral water: sulfate sodium-magnesium-calcium concentration of sulfate 2.6 g/l and mineralization 3.1 g/l or the same sulphate sodium-magnesium-calcium, but with the addition of the trace element silicon with a concentration of 150 mg/L.

The use of mineral waters was carried out in the course of 21-24 days (common in balneology time). The volume of injected intragastrically mineral water, i.e., 3.0 to 3.5 ml was carried out on the basis of terms of body weight of the experimental animal and correspond to the same quantities that are used in the treatment of balneological practice.

The difference of our method is that used drinking mineral water sulphate sodium-magnie the ment of silicon with a concentration of 150 mg/l used in the early post-radiation period and their action is aimed at the protection of the testes from the effects of radiation treatment: radiation therapy of cancer patients, in regions with high background radiation, long-term radiation exposure in uranium mines and other

The effectiveness of these waters was assessed by histological, ultrastructural, and cytogenetic, morphological and radioimmunological parameters.

The basis for use of mineral waters was a native data capacity of their minerals and ion-salt framework to regulate intracellular metabolic processes, to accelerate the adaptation, to reduce the level of free radical oxidation, to reduce the number of pathological mitoses in radiosensitive tissues (small intestine, red bone marrow) and excrete toxic products. Adding silicon sulfate in water enhances the security and stability of cell membranes and contributes to the accumulation of glycogen in the liver. To prevent the development of more persistent irradiation of violation and correction of already existing primary changes at the molecular level it is advisable to use mineral water in the earliest periods after irradiation. This leads to early removal of toxic products formed, to reduce disturbance of cellular and intracellular membranes, bullseyeing the development of dystrophic and degenerative processes in the spermatogenic epithelium of the testes.

Description of the way

Prevention of the consequences of the total radiation exposure (dose 1-2 G) was started after 5-6 hours after exposure. Animals (non-linear white rats-males) was administered intragastrically by syringe through a needle with an olive on the end of 3.0 - 3.5 ml of one of the following types of water: sulfate sodium-magnesium-calcium concentration of sulfate 2.6 g/l and mineralization 3.1 g/l or the same sulphate sodium-magnesium-calcium water, but with the addition of the trace element silicon with a concentration of 150 mg/L.

As control was used animals that had been irradiated in the same doses, but instead of mineral water they were introduced with ordinary tap water. All animals were kept in standard vivarium conditions and were under observation during the whole experiment. Evaluating the effectiveness of courses of drinking mineral water carried through 29-30 and 35-37 days after radiation exposure.

Example 1. In the group of 10 rats after a single total radiation dose of 2 Gy daily before the morning feeding was introduced 3.0 ml syringe through a needle with an olive on the end of sulphate sodium-magnesium-calcium water with the addition of silicon to 150 mg/l In rasulala: number of convoluted seminiferous tubules with the highest content of germ cells (3-4 generation) was significantly higher (P< 0.05), and the number of tubules with reduced number of cells (1-2 generation) - significantly less (P < 0,01). Decreased the degree of degenerative changes in germ cells, in particular the phenomena of their vacuolisation the destruction of cellular and intracellular membranes. Index of spermatogenesis remained at a higher level compared to control (P < 0,05).

Example 2. In the group of 10 rats after a single total radiation dose of 2 Gy daily before the morning feeding was introduced 3.0 ml syringe through a needle with an olive on the end of sulphate sodium-magnesium-calcium water. As a result of the course of drinking this water was shown a distinct tendency to increase the number of convoluted seminiferous tubules with 3-4 generations of cells and reduced their number from 2 generations. Decreased number of endothelial cells. Index of spermatogenesis remained above control levels, but lower than under the action of sulfate water with the addition of silicon.

Example 3. In the group of 12 rats after a single total radiation dose of 1 G daily before the morning feeding was introduced 3.0 ml syringe through a needle with an olive on the end of the natural sulphate sodium-magnesium-calcium water. In providen the OK was significantly higher than in control (P < 0.05) and the phenomenon of shedding cells and zapustevanie tubules manifested weaker, and the index of spermatogenesis was clearly higher than the control (P < 0,01).

Example 4. The group of 12 rats after a single total radiation dose of 1 G daily before the morning feeding was introduced 3.0 ml syringe through a needle with an olive on the end of sulphate sodium-magnesium-calcium water with the addition of silicon. As a result of the course of drinking this water, the number of convoluted seminiferous tubules with 3-4 generations of cells was higher than in control (P < 0.05), and the phenomenon of shedding cells into the lumen of the tubules were expressed weaker not only in comparison with the control, and compared with the effect of sulfate water without silicon. Electron-microscopically, it was noted the best preservation of the basal membrane of convoluted seminiferous tubules. Index of spermatogenesis remained above control levels (P < 0,01).

The proposed method of secondary prevention of disorders of spermatogenesis when exposed to radiation was applied at 110 outbred rats male. As a result of its use in the testes revealed a higher number of convoluted seminiferous tubules with the maximum number of generations pole shedding of cells into the lumen of the tubules was significantly less than in the control group of animals treated tap water. Reduces the severity of damage from cellular and intracellular membranes. To a lesser extent were changes in interstitial cells, ultrastructural analysis revealed the activation of processes of adaptive adjustment.

The proposed method of secondary prevention prevented and, thereby, limits the development of the main post violations in the testes. As a result of application of this method decreases the degree of destructive processes in the spermatogenic epithelium remains significantly higher number of germ cells, including their most Mature form (Spermatid and sperm), are activated adaptation processes, and the index of spermatogenesis remains at a higher level compared to control.

The most pronounced prophylactic effect identified by the effect of sulphate sodium-magnesium-calcium water with the concentration of sulfate 2.6 g/l and mineralization 3.1 g/l with the addition of 150 mg/l of silicon.

The method of secondary prevention of disorders of spermatogenesis when exposed to radiation, characterized in that the animals early after radiation irradiation is Fatou sodium-magnesium-calcium water with the concentration of sulfate 2.6 g/l and mineralization 3.1 g/l, or the same sulphate sodium-magnesium-calcium, but with the addition of the trace element silicon with a concentration of 150 g/L.

 

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