Gel for the treatment of wounds

 

(57) Abstract:

The invention relates to medicine. To reduce treatment time RAS features gel on gelatin-glycerol-based as a medicinal product containing 0.1 to 0.6% integrated product isolated from the cytoplasm of Staphylococcus aureus, having a peculiar effect on the pathogen. Selected optimum ratio of therapeutic doses and gel bases facilitate the active contact of the drug with wound tissue, which provides faster healing of wounds and reduces the treatment time. table 1.

The invention relates to medicine and veterinary medicine, and in particular to methods of treatment of wounds.

Known ointment containing drug gentamicin sulfate ointment and a basis in the ratio (grams) of 0.1:99,9 respectively, used for the treatment of pyodermia (M. D. Mashkovsky "Drugs", M,Medicine, 1993, so 2, page 288) prototype.

However, the known ointment has a nonspecific effect and requires long-term use.

The aim of the invention is the reduction of treatment time of pyodermia.

This goal is achieved by the use of "Sonatina" with specificamente drug use daily microbial culture, grown on agar of Hottinger. To obtain the "Tomatina" microbial mass of Staphylococcus containing 1 ml 250 billion microbial cells are subjected to degradation in the mechanical disintegrator (L-17) using the beads Bollotini N 11. After removal of intact microbial cells and cell walls by centrifugation (at 8000-10000 rpm, 30 minutes) get the cytoplasmic fraction. Electron microscopic examination shows complete absence of intact cells and cell walls in the cytoplasm.

The chemical composition of the cytoplasm: protein (58-60%),nucleic acids (11-13%), reducing sugar (11-13%), lipids (9-10%). In immunoelectrophoresis cytoplasm forms with homologous serum 7 bands of precipitation, electrophoresis in polyacrylamide gel is divided into 19 components.

Example 1. "Somethin" separated from the cytoplasm sequentially by acid precipitation at pH 3.5, followed by presidenial at pH 4.5. To remove protein impurities are trypsinization for 4 hours, allowing you to remove from the drug for more than 60% protein. Chemical composition Sonatina": protein (40-45%), nucleic acids (30-34%), reducing sugar (13-17%) and lipids (5-6%). In immunoelectrophoresis "Somethin" on the SJ 9 components. The drug cialiswhat against saline for 24 hours (d=0,045 mm, Visking dialysis tubing "SERVA"), and then freeze-dried. Gelfiltration drug on Sephadex g-75 determines that the output curve has two asymmetric peak, and the peak fraction 1 is identical to the original trypsinization fraction chemical composition, immunogenicity and serological activity. Fraction 2 peak poorly immunogenic and serologically inactive.

Thus "Somethin composition is complex, including substance nucleic nature, protein, polysaccharides and lipids, drug soluble in physiological solution pH 7,0-7,8.

Research on animals shows that the immunity created "Sonatina", is peculiar, because it creates a protection only against Staphylococcus. The basic mechanism of the immune system caused by the activation of intracellular digestion phagocytosing staphylococci. In the abdominal cavity immunized and infected animals there is a rapid influx of leukocytes and continuous decrease in the number of living microbes. Along with this process Somethin" is an activator of T-helper cells, which enhance the synthesis of specific antibodies.

ogie and hygienic regulation of biopreparations (NICTSP). Installed the complete absence of sensibilizatsii body substance "Sonatina" when applied to skin.

Example 2. Gel for the treatment of wounds produced as follows: gelatin soaked in distilled water, heated to dissolve, and after cooling to a temperature equal to 40oC add "Somethin" with glycerin and boric acid.

To prepare 100 g of gel containing 0.1-0.6% of "Sonatina", take the ingredients in the following quantities (g):

"Somethin" is 0.1 and-0.6

Glycerin - 25,0

Gelatin - 10,0

Boric acid and 2.0

Distilled water to 100.0

A method of treating wounds using "Sonatina" studied in experiments on animals. Use of adult dogs of both sexes.

Example 3. Under observation taken 5 animal groups in three dogs in each breed: German shepherd, Cocker Spaniel, French bulldog, great Dane, Neapolitan mastin, Royal poodle). Dogs three groups receiving treatment with a gel containing "Somethin in concentrations 0,1,0,3 and 0.6%. The fourth group of control (animals put gel base without "Sonatina"). Dogs of the fifth group to compare the effectiveness of the treatment applied is 0.1% gentamitsinovoy ointment. Srednes the s S. aureus, S. epedermidis, S. intermedius, Str.pyogenes

After debridement grease gel containing "Somethin in concentrations of 0.1, 0.3 and 0.6 percent, dried 5-7 minutes and apply a sterile dressing. Ligation spend every day. The results of the treatment of wounds in the table.

As can be seen in the table, treatment with a gel containing different concentrations of "Sonatina" leads to positive effect. Redness and swelling in the lesions reduced by treatment with 0.1% Satinover gel next day, when treating with 0.3% gel in 0.5 days and 0.6% gel through 0.6 days, and 0,1% gentamitsinovoy ointment on the 4th day. Purification of purulent-necrotic tissue occurs in the treatment of 0.1% Satinover gel on the 5th day, 0.3% and 0.6% gel on the 4th day, and 0,1% gentamitsinovoy ointment on the 7th day. The appearance of granulation observed in the treatment with 0.1% Satinover gel on the day 3, 0.3% and 0.6% gel on the 2nd day. Treatment with 0.1% gentamitsinovoy ointment leads to the appearance of granulations on the 4th day. Filling the wounds with granulation occurs on the 5th day for lubrication of 0.1% Satinover gel and after 4 days in the treatment of 0.3% and 0.6% gel. In the treatment of 0.1% gentamitsinovoy ointment granulation tissue fills the wound n is on the 5th day, and of 0.1% gentamitsinovoy ointment on the 8th day. Wound healing is observed on the 22nd day when lubricating RAS 0,1% Satinover gel, on the 20th day in the treatment of 0,3-0,6% gel and on 28th day of treatment with 0.1% gentamitsinovoy ointment.

Dogs in the control group, which is applied on the affected skin gel base without "Sonatina", therapeutic effect is not observed.

The treatment results show the advantages of the proposed method of treatment of wounds gel containing 0.1-0.6% of "Sonatina", compared with the 0.1% gentamitsinovoy ointment. The treatment of purulent wounds of the proposed method (gel containing 0.1-0.6% of "Sonatina") is more effective than treatment with 0.1% gentamitsinovoy ointment, as the treatment time is reduced on average 8 days.

Thus, therapeutic effect of the gel is achieved due to the specific impact Sonatina" on the protective properties of the wounded tissue. Optimal ratio of a therapeutic dose and a gel base. The proposed gel Foundation promotes the active contact of the drug with the wounded tissues, which speeds up the healing of wounds.

We offer gel containing "Somethin", has no side effects on the body of the dog.

Gel for the treatment of wounds, comprising l is the integrated product isolated from the cytoplasm of Staphylococcus aureus, consisting of nucleic acid (30 - 34%), protein (40 - 45%), reducing sugars (13 - 17%) and lipids (5 - 6%), and gel-based contains glycerin, gelatin, boric acid and distilled water in the following proportions of components:

The integrated product isolated from the cytoplasm of Staphylococcus aureus - 0,1 - 0,6

Glycerin - 25,0

Gelatin - 10,0

Boric acid and 2.0

Distilled water Up to 100.0

 

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