The method of obtaining food

 

(57) Abstract:

The invention relates to the field of food industry. For receiving food product from prirodoslovni natural raw materials as enzymatic hydrolysate kollagensoderzhaschego tissue using a food product "Collabin", including amino acids, polypeptides, vitamins, water and fat soluble vitamins, minerals and residual moisture. On the basis of the "Calamina prepare a nutrient medium containing sodium chloride, lactose, agar-agar and purified water. Cultivate bifidobacteria or lactobacilli. The finished product contains 1 to 108- 1 109viable cultures. The product is enriched with biologically active polypeptides with a molecular mass of up to and above 2000 Da, trace elements, organic and unsaturated fatty acids. 7 C.p. f-crystals, 4 PL.

The invention relates to the field of food industry, in particular to a method of obtaining biologically active compounds on the basis of strains of bifidobacteria and lactobacilli, intended for the prevention of gastrointestinal diseases and for specialized nutrition for athletes.

A method of obtaining food product which ISM is servicia in certain weight ratios (RF patent N 2146484, class A 23 L 1/312, from 07.08.98).

However, the known method is suitable only in laboratory conditions for obtaining biologically active compounds.

The technical nature closest to the proposed invention is a method of obtaining a food product, which consists in the preparation of the nutrient medium on the basis of enzymatic hydrolysate kollagensoderzhaschego tissues enriched in chloride of sodium, lactose, agar-agar and purified water, sterilization of the nutrient medium and then cooled to a temperature of cultivation of microorganisms, making the culture medium was pure cultures of bifidobacteria or lactobacilli and their subsequent cultivation to the finished product (see RF patent N 2136167, CL A 23 C 9/12, from 02.06.98).

However, in the food product, obtained according to the method, not identified organic and unsaturated fatty acids, water - and fat-soluble vitamins and minerals are essential in the diet of man.

The technical result is the creation of a method of producing a food product containing a complete set of amino acids, vital organic and unsaturated fatty acids, water - and fat-soluble vitamins and food product, consisting in the preparation of the nutrient medium on the basis of enzymatic hydrolysate kollagensoderzhaschego tissues enriched in chloride of sodium, lactose, agar-agar and purified water, sterilization of the nutrient medium and then cooled to a temperature of cultivation of microorganisms, making the culture medium was pure cultures of bifidobacteria or lactobacilli and their subsequent cultivation to the finished product, according to the invention as enzymatic hydrolysate kollagensoderzhaschego tissue using food protein product, including a full set of amino acids, polypeptides, vitamins, water and fat soluble vitamins, minerals and residual moisture, in addition, dietary protein product is taken in the following ratio, wt.%:

Amino acids - 30-50

Di-, tri - and tetrapeptide - 35-55

The polypeptides are not above 2000 Yes - 11-16

The polypeptides of more than 2000 Yes - 0,05-0,9

Vitamins - 0,05-0,9

Trace elements - 0,05-0,5

Residual moisture - 2-5

In addition, preferably as a food protein product to use "Collabin" beyond 9186-002-18045931-98, and the enrichment of chloride, sodium and lactose to produce at room temperature with subsequent Nagravision components, vol.%:

"Collabin" - 255

Sodium chloride - 5,02

Lactose - 10,04

Agar-agar - 1,00,5

Water (purified) - the Rest is up to 1 litre

In addition, preferably the nutrient medium to prepare concentrations of hydrogen ions pH = 7,20,2, and sterilization of the nutrient medium to produce at t = 1128oC and a gauge pressure of 0.05-0.1 MPa during 2510 minutes, while the introduction into the nutrient medium pure cultures of bifidobacteria or lactobacilli to produce gradually over several generations, and the first step dose dry preparation of pure cultures to make into a test tube with a nutrient medium for growth and reproduction, and in the subsequent steps to produce further reproduction in large volumes of nutrient medium, with a dose of dry preparation of pure cultures contains 1108-1109microorganisms, and the temperature of the cultivation of microorganisms to maintain in the range of 35-38oC.

The invention consists in that the use of "calamina" food as enzymatic hydrolysate kollagensoderzhaschego tissues allowed us to obtain a food product, in addition to containing high titers of bifidobacteria and lactobacilli are also vital for a person of their IU"ptx2">

The method of obtaining food is to prepare nutrient medium on the basis of enzymatic hydrolysate kollagensoderzhaschego fabrics, which use food protein product, which should contain a complete set of amino acids, polypeptides, vitamins, water - and fat-soluble micronutrients.

Preferably, the food protein product had the following ratio of components, wt.%:

Amino acids - 30-50

Di-, tri and tetrapeptide - 35-55

The polypeptides are not above 2000 Yes - 11-16

The polypeptides of more than 2000 Yes - 0,05-0,9

Vitamins - 0,05-0,9

Trace elements is 0.05 to 0.9

Residual moisture - 2-5

So meet the requirements of food protein products "Collabin" on THE other-9186-002-18045931-98.

The nutrient medium on the basis of the "Calamina" enrich chloride of sodium, lactose, agar-agar and purified water, then sterilized, cooled to a temperature of cultivation of microorganisms and contribute to the nutrient medium pure culture of bifidobacteria or lactobacilli.

Cultivation of microorganisms produce in thermostatic volume.

The enrichment of chloride, sodium and lactose produced at room temperature and then heat the attachment of components, vol.%:

"Collabin" - 255

Sodium chloride - 5,02

Lactose - 10,04

Agar-agar - 1,00,5

Water (purified) - the Rest is up to 1 litre

A nutrient medium is prepared to concentrations of hydrogen ions pH = 7,2 0,2, and sterilization it is carried out at a temperature of 112 8oC and a gauge pressure of 0.05 - 0.1 MPa for 25 to 10 minutes.

The introduction into a nutrient medium pure cultures of bifidobacteria produce gradually over several generations, and the first step dose dry preparation of pure cultures, such as strains of bifidobacteria: B. longum 379 B or B. bifidum 791 and strains of lactobacilli: L. casei 37, L. plantarum 38, L. fermentum 39, dose dry preparation of pure cultures contribute in a test tube with a nutrient medium for the growth and reproduction of large volumes of nutrient medium, with a dose of dry preparation of pure cultures contains 1108- 1109of microorganisms.

The temperature of the cultivation of microorganisms supported in the range of 35-38oC.

Example cooking food.

To implement the first generation of the ampoule with liofilizirovannam strain of bifidobacteria or lactobacilli nadelwald at the base of the stem, wipe it with cotton wool moistened with alcohol, burn the place Napili sterile nutrient medium. If lyophilized strain is penicillin bottle, aluminum bottle cap, wipe the Vatican with alcohol, flamborough in the flame of the burner, remove the forceps cap, treated with alcohol and flamborough tube, open it in the flame of the burner and sterile bring in a bottle the required amount of the nutrient medium. The contents of the ampoule or vial sterile transferred into two tubes (or bottle) with sterile nutrient medium, poured on 30 ml, and mix thoroughly. The test tubes are incubated at a temperature of 37.5 0,5oC for 20 2 hours. Thus obtained culture of bifidobacteria first generation used for secondary transplantation (second generation) by making at least 5% of the contents of the two tubes of the total volume of sterile nutrient medium, poured into special containers, depending on the required amount and timing of the cooking culture of the third generation. Crops maintained at a temperature of 37 1oC for 18 to 20 hours before intensive cloud environment, then use it for cooking culture of the third generation. The contents of the vial thoroughly mixed and transferred into a sterile large capacity (1.5 to 2 l), in which sterilmeden this time the food product is ready to use. It contains a complete set of amino acids, biologically active polypeptides, vitamins, water - and fat-soluble micronutrients, as well as the living cells of bifidobacteria and lactobacilli.

In table. 1 shows the qualitative and quantitative compositions of amino acids derived food product.

In table. 2 shows the qualitative and quantitative compositions of vitamins.

In table. 3 - organic and unsaturated fatty acids, and table. 4 presents microbiological indicators.

Thus in the proposed method provides the food product containing the full set of amino acids, vital organic and unsaturated fatty acids, water - and fat-soluble vitamins and minerals from prirodoslovni natural product, as well as the living cells of bifidobacteria and lactobacilli.

1. A method of obtaining a food product, which consists in the preparation of the nutrient medium on the basis of enzymatic hydrolysate kollagensoderzhaschego tissues enriched in chloride of sodium, lactose, agar-agar and purified water, sterilization of the nutrient medium and then cooled to a temperature of cultivation of microorganisms, unessecery finished product, characterized in that as enzymatic hydrolysate kollagensoderzhaschego tissue using dietary protein, including amino acids, polypeptides, vitamins, water and fat soluble vitamins, minerals and residual moisture taken in the following ratio, wt .%:

Amino acids - 30 - 50

Di-, tri - and tetrapeptide - 35 - 55

The polypeptides are not above 2000 Yes - 11-16

The polypeptides of more than 2000 Yes - 0,05-0,9

Vitamins - 0,05 - 0,9

Trace elements - 0,05 - 0,5

The residual moisture of 2 to 5

2. The method according to p. 1, characterized in that the quality of food protein product use "Collabin".

3. The method according to p. 1, characterized in that the enrichment of chloride, sodium and lactose produced at room temperature followed by heating after making agar-agar to its complete dissolution.

4. The method according to p. 3, characterized in that the enrichment is carried out at the following ratio of components,%:

Collain - 25 5

Sodium chloride - 5,0 2

Lactose - 10,0 4

Agar-agar - 1,0 0,5

Water (purified) - the Rest is up to 1 litre

5. The method according to p. 4, wherein the nutrient medium is prepared to concentrations of hydrogen ions pH 7,2 0,2.

6. The way p is 5 - 0.1 MPa within 25 10 min.

7. The method according to p. 1, characterized in that the introduction into a nutrient medium pure cultures of bifidobacteria or lactobacilli produce gradually over several generations, and the first step dose dry preparation of pure cultures contribute in a test tube with a nutrient medium for growth and reproduction, and in the subsequent steps produce a further multiplication of large volumes of nutrient medium, with a dose of dry preparation of pure cultures containing 1 to 108- 1 109of microorganisms.

8. The method according to p. 1, characterized in that the temperature of the cultivation of microorganisms supported in the range of 35 - 38oC.

 

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SUBSTANCE: method involves pasteurizing whole milk; cooling to fermentation temperature; introducing starter including bifidus bacteria and lactic streptococcuci; introducing vitamin additive; mixing; fermenting until live cell content in product is 109-1010 CFU/cm3 and acidity is 80-1000T; cooling; mixing and bottling. Vitamin additive is concentrated citrus juice, or Jerusalem artichoke juice, or Jerusalem artichoke powder juice, or Jerusalem artichoke syrup. Concentrated citrus juice is introduced in an amount of 30-50 ml per 1 l of milk, Jerusalem artichoke juice, powder juice or syrup is introduced in an amount of 0.3-0.5% by volume of milk. Method allows useful components to be extracted to maximal extent from basic product.

EFFECT: improved quality, increased assimilation of useful components by people of any age owing to development of natural microflora by product itself.

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