Biological preparation to enhance the productivity of bees

 

(57) Abstract:

The invention is intended for the production of biologically active probiotic supplements to stimulate the physiological functions of the bees. The preparation contains as biologically active substances dry backass Bifidobacterium Bifidobacterium globosum VGNKI BF-4/DEPT, dry backass strain Streptococcus Streptococcus faecium VGNKI 27/11-IN-DEPT. It also contains ascorbic acid, carbohydrates, aluminum oxide, aluminum hydroxide and the filler. The biological product is dissolved in a small amount of boiled water. Add in the sugar syrup at the rate of 1 g of the drug in 200 ml of sugar syrup. Feed it to the bees twice. First time with the first feed and the second time in 7-10 days based on 100 ml of the prepared syrup drug on one street. The drug has a high growth stimulating effect on the body of bees, helps to increase their physical activity and commodity output. 2 C.p. f-crystals, 1 table.

The invention relates to the field of biotechnology and veterinary medicine and for the production of biological products, in particular biologically active probiotic supplements to stimulate the physiological functions of bees.

the example amino acids, yeast or blue-green Alga Chlorella (SU N 793556, 07.01.81; SU N 829088, 25.01.81; SU N 546337, 21.03.77).

However, the known additives do not contain sufficient amounts of protein, vitamins, biologically active substances and therefore would not significantly affect the productivity of bees.

The closest analogue is the drug to stimulate the breeding of bees and increase their resistance to various extreme factors containing the biologically active substance is a mixture of ecdysone and vitamin B12and the filler is a mixture of sugar, starch and stearic acid (RU N 2034504, 10.05.95).

The disadvantage of the nearest analogue is that it mainly regulates the reproduction of bees and no significant impacts to these physiological functions of bees, as a summer activity and productivity of developing uterine milk and honey.

One of the modern approaches to increase the productivity of agricultural animals and poultry is the use of probiotics drugs, which include living microorganisms: representatives of normal microflora of the intestinal tract or saprophytes, living in the environment. Unlike antibiotics and other biologically activesoftware (Antipov C. A., Subbotin C. M. Effectiveness and prospects of application of probiotics // "veterinary medicine", 1980, No. 12, S. 55-57).

The mechanism of action of drugs based on adhesive and antagonistic properties of bacterial probionts, displacing from the composition of the intestinal population of conditionally pathogenic microorganisms and nonspecific controlling the redundancy of their growth.

It is known that in addition to their protective function of the intestinal normoflora takes an active part in the processes of microbial intestinal digestion, break down proteins to amino acids and peptides, produce acids, vitamins, bacteriocins and biologically active substances, as well as stimulate the nonspecific resistance of the organism.

Probiotic preparations for the normalization of intestinal biocenosis bees has not previously been used, although probably antagonistic relationship between opportunistic and normal intestinal microflora, the bees are.

Object of the invention is the creation of an effective probiotic preparation to stimulate the vitality of the bees and increase their productivity.

The technical result of the invention consists in obtaining a probiotic preparation with active Biol the spine.

The invention consists in the following: biological preparation to enhance the productivity of bees contains as biologically active substances dry backass Bifidobacterium Bifidobacterium globosum VGNKI N CF-4/DEPT, dry backass strain Streptococcus Streptococcus faecium VGNKI N 27/11-IN-the DEPOT, but also contains ascorbic acid, carbohydrates, aluminum oxide, aluminum hydroxide and a filler, in the following ratio of components per 1 kg of the drug:

Dry backmask Bifidobacterium gtobosum VGNKI N CF-4/DEPT content 30-1000 billion microbial cells (M. K.) 1 g - 2,0 - 4,0 g

Dry backmask strain of Streptococcus faecium VGNKI N 27/11-IN-DEPT with the content of 30-1000 billion, M. K. 1 g - 2,0 - 4,0 g

Carbohydrates - 20 - 40 g

Aluminum oxide is 10 to 30 grams

Aluminium hydroxide - 100 - 300 g

Ascorbic acid - 100 - 300 g

Filler - Rest

From carbohydrates biological product contains lactose or sucrose, and fillers - food sugar.

Strains of Bifidobacterium globosum VGNKI N CF-4/DEPT and Streptococcus faecium VGNKI N 27/11-IN-DEPT-known and are part of the preparation "Streptavidin", which is used for the correction of intestinal biocenosis in warm-blooded vertebrate animals and birds (RU N 2086248, publ. 10.08.97). However, unexpectedly it was found that well-known sreenu, proved effective for the stimulation of physiological functions in bees.

The strain of Bifidobacterium Bifidobacterium globosum VGNKI N CF-4/DEPT and strain of Streptococcus faecium N 27/11-IN-DEPT have high growth and biological activity in the body of bees, capable of lactic acid and other biologically active substances. The synergistic actions of this combination lies in the fact that streptococci, actively absorbing oxygen, improve habitat for anaerobic bacteria. Ascorbic acid is introduced into the composition of the drug, acts as an additional component to bind molecular oxygen and as a vitamin component.

The biological product is dissolved in a small amount of boiled water, add in the sugar syrup at the rate of 1 g of the drug in 200 ml of sugar syrup and feed it to the bees twice: once with the first feed and the second time in 7-10 days based on 100 ml of the prepared syrup drug on one street.

The invention is illustrated by the following examples.

Example 1. Probiotic preparation is produced by a separate submerged cultivation of strains of bifidobacteria, streptococci liquid cus faecium VGNKI N 27/11 IN-DEPT and Bifidobacterium Bifidobacterium globosum VGNKI N CF-4/DEPT.

During cultivation of bacteria for 16+2 h biomass yield of each species of bacteria of at least 10 billion live microbial cells in 1 ml culture suspension.

The obtained culture was concentrated in 10-15 times depending on the density of the culture of sediments washed from the products of metabolism in the separators or microfiltration units.

Concentrated bacterial suspension is dried by sublimation method according to the conventional technology.

In the resulting dry semi-finished products of each strain contains at least 100 billion living microbial cells per gram.

Next dry backass each strain are mixed in a ratio of 1:1 and add carbohydrate, oxide and aluminium hydroxide, ascorbic acid and filler (sugar food, sugar), with the following ratio of 1 kg:

Dry backmask strain of Streptococcus content of 10 to 3000 billion, M. K. 1 g - 3 g

Dry backmask strain of bifidobacteria content 10-3000 billion, M. K. 1 g - 3 g

Carbohydrate (lactose or sucrose) - 30 grams

Aluminum oxide - a 20 g

Aluminium hydroxide - 200 grams

Ascorbic acid 200 g

Filler - Up to 1 kg

The composition allows the ratio from 60 million up to 6 billion, M. K., and ascorbic acid to 0.2 grams per gram.

Example 2. Obtained according to example 1 of the biological product have biostimulating activity. Define biostimulating activity of the drug with the total concentration of bacteria 60 million M. K., and 2 billion m K. in one gram.

As a biological model for testing drug use bee on low apiaries, not perform the delivery of the commodity output per colony.

Bee colonies in one apiary divided into three groups - two experimental and one control with an equal number of streets in each hive (6-8 lanes).

The drug concentration of microbial cells 60 million and 2 billion in one gram dissolved in a small amount (20-50 ml) of boiled water and mixed with sugar syrup at the rate of 1 gram of the drug in 200 ml of sugar syrup and fed his bee colonies the first and the second experimental group, respectively.

The prepared syrup drug ask the bees twice - first time with the first feed and the second time in 7-10 days based on 100 ml of the prepared syrup drug on one street.

Bee the third group used as control: they boards of drug activity summer bees (number of departures per min), the number of the grown brood in hundreds of cells on the family and on the yield as% of plan (plan is 0.2 kg of Royal jelly per colony and 10 kg of marketable honey). The results presented in the table.

As can be seen from the table above, the drug has a growth stimulating effect on the body of bees, contributes to their physiological activity and commodity output.

The drug has no negative side effects on the body of bees and does not reduce the quality of commercial products, all samples of honey from bee colonies experimental groups was consistent with the requirements of GOST in terms of quality.

1. Biological preparation to enhance the productivity of bees containing a biologically active substance and a filler, characterized in that it further comprises ascorbic acid, carbohydrates, aluminum oxide, aluminum hydroxide, and as biologically active substances it contains dry backass Bifidobacterium Bifidobacterium globosum VGNKI BF-4/DEPT, dry backass strain Streptococcus Streptococcus faecium VGNKI 27/11-IN-DEPT in the following ratio of components per 1 kg of the drug:

Dry backmask Bifidobacterium globosum VGNKI BF-4/DEPT maintained with the.m.to. 1 g - 2,0 - 4,0 g

Carbohydrates - 20 - 40 g

Aluminum oxide is 10 to 30 grams

Aluminium hydroxide - 100 - 300 g

Ascorbic acid - 100 - 300 g

Filler - Rest

2. The drug under item 1, characterized in that from carbohydrates it contains lactose or sucrose.

3. The drug under item 1, characterized in that the filler it contains food sugar.

 

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