Method for the production of baker's yeast

 

(57) Abstract:

The invention relates to the microbiological industry, in particular the production of Baker's yeast, enriched physiologically active substance. The method involves a multi-stage culturing yeast under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus, growth stimulants and source of iodine. The source of iodine periodically bring in the culture fluid when the dissolved oxygen concentration in the culture fluid of 1.5 - 3.5 mg/L. the First portion of the source of iodine applied after the exponential phase of yeast growth. The method allows to reduce the consumption of the source of iodine and get the yeast biomass containing 600 mg of iodine in 1 kg of dry matter of the yeast. 1 C.p. f-crystals.

The invention relates to the microbiological industry, in particular the production of Baker's yeast, enriched physiologically active substance is iodine. Based on the biomass of these yeast can be developed therapeutic and prophylactic drugs, food and feed additives.

A method of obtaining baking yeast, enriched with iodine. The method involves the introduction of dragieva is b.%, as the oxidant is hydrogen peroxide at a concentration of 2-6% of dry substances of yeast or ozone, you get a yeast biomass containing 600 mg of iodine in 1 kg of dry matter of the yeast [1].

The disadvantage of the proposed method is the complexity of its implementation, as in addition to iodine, you must enter additional components, for the use of ozone requires additional equipment.

The closest in technical essence and the achieved result to the present invention is a production method of baking yeast, enriched with iodine, by multi-stage cultivation in conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus, growth stimulants and source of iodine, periodically supplied into the culture fluid. As a source of iodine use potassium iodide or potassium Iodate. By yeast biomass with iodine content of up to 600 mg/kg of dry matter of the yeast [2].

The disadvantage of this method is the high consumption of the source of iodine.

The technical essence of the invention is to reduce the consumption of iodine by improving the efficiency of its consumption by the yeast.hashannah iodine, by multi-stage culturing them under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus, growth stimulants and source of iodine, periodically supplied into the culture fluid according to the invention a source of iodine contribute when the oxygen concentration in the culture fluid of 1.5-3.5 mg/l, while the first portion of the source of iodine applied after the exponential growth phase of the yeast.

In the process of culturing yeast dissolved oxygen in the culture fluid changes. As the consumption of nutrients concentration increases.

It was established experimentally that the optimal iodine intake in the yeast occurs when the dissolved oxygen concentration in the culture fluid of 1.5-3.5 mg/L. This is because in this period the yeast are most susceptible to the introduced additives and, in particular, to the source of the trace element (source of iodine). Therefore, at lower concentrations of the source of iodine, periodically supplied into the culture fluid in the cultivation process, the iodine content in 1 kg of dry matter of the yeast remains at the same level as in the known method. The first portion of the source of iodine CE is white yeast cells.

The method is as follows.

Multi-stage cultivation of the yeast Saccaromyces cerevisiae carried out sequentially in the apparatus capacity 8.30 and 100 m3the conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus and growth stimulants. The first portion of the source of iodine contribute in the culture liquid after the exponential phase of yeast growth when the oxygen concentration in the culture fluid of 1.5-3.5 mg/l, a further source of iodine periodically make with the indicated concentrations of dissolved oxygen during the entire cultivation process the process of culturing yeast for 15 h, the resulting yeast is separated from the culture fluid.

Example

The cultivation of the yeast Saccharomyces cerevisiae carried out sequentially in the apparatus capacity 8.30 and 100 m3the conditions of aeration on a commonly used nutrient medium containing sources of carbon, nitrogen, phosphorus, growth stimulants and source of iodine. The first portion of the source of iodine applied after the exponential phase of yeast growth while achieving the dissolved oxygen concentration in the culture fluid of 2.5 mg/l, potassium iodide contribute to the concentration of iodine is of Rogga carried out for 15 hours After culturing the yeast is separated from the culture fluid, get the yeast biomass containing 600 mg of iodine in 1 kg of dry matter of the yeast.

Literature

1. RF patent N 2119952, With 12 N 1/18, 1998

2. RF patent N 2104302, With 12 N 1/18, 1998 (prototype).

1. Method for the production of Baker's yeast, enriched with iodine by multi-stage culturing them under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus, growth stimulants and source of iodine, periodically supplied into the culture fluid, characterized in that the source of iodine contribute when the oxygen concentration in the culture fluid of 1.5 - 3.5 mg/l

2. The method according to p. 1, wherein the first portion of the source of iodine applied after the exponential phase of yeast growth.

 

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EFFECT: simplified method, wider range of use and improved quality of ready product.

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