The method of treatment of diabetes

 

(57) Abstract:

The invention relates to medicine, namely to endocrinology, and can be used in the treatment of diabetes. Recipient previously administered polyacrylamide gel, followed by transplantation into it the beta cells of the pancreas. The method can significantly reduce the dose of insulin. This increases the efficiency of treatment of the disease.

The invention relates to medicine, namely to the treatment of diabetes, mainly insulin, by transplantation of beta cells of the pancreas.

There is a method of treatment of diabetes, according to which produce implantation of cells benign insulinoma with material containing cells of the pancreas, are implanted into the straight muscle of the abdomen (RF patent N 2004247).

The disadvantages of this method include the problems arising in connection with the fight with a predominance of fibroblast growth when using transplantable culture cells and the need for accurate performance monitoring insulin specific fraction of the cell culture insulinoma, which is due to the fact that as the pulse is LASS="ptx2">

The closest to the proposed method according to the technical essence and the achieved result is a method of treatment of diabetes by transplanting material containing cells of the pancreas (RF patent N 2135193).

A method of treating diabetes, insulin dependent mainly, carried out using a material containing cells of the pancreas of mammals obtained by use of the phenomenon of migration of cells.

Material containing cells of the pancreas transplanted in various organs and tissues; intramuscularly, in a straight muscle of the abdomen, the liver (in the parenchyma or portal vein), in the pulp of the spleen, splenic artery, into the cavity of the peritoneum, omentum, in a specially created muscle pocket.

The disadvantage of this method of treatment of diabetes is the brevity producing cells of the donor of insulin in the body of the recipient because of the effect of exclusion of heterogeneous cells, which results in significant immunosupresivny therapy.

The essence of the proposed method of treatment of patients with diabetes is the duration and produtsirovaniya the introduction of patient polyacrylamide gel subcutaneously, followed by transplantation of cells formed in the capsule.

Material for transplantation of cells from the pancreas of mammals (newborn piglets, rabbits, Mature green monkeys). The cultivation is carried out with the use of standard environments and solutions. To maintain cells in an active state, use the gentle enzyme treatment of the pancreas, which consists in alternating contact of the fabric with enzyme and nutrient medium. As a result of processing stages fragments of pancreatic tissue and cells contribute to the culture vessels without centrifugation. Moreover, the disaggregation of tissue conduct of 0.1-0.25% solution of trypsin and himopsina in different sequences depending on the donor material. Complete enzymatic treatment of the tissue during its contact with the environment using bulb "biotech-m, providing adjustable stirring suspension on a magnetic table.

The obtained cell material is administered to the recipient in connection-woven capsule, which is formed previously introduced subcutaneously polyacrylamide gel. The number of cells depends on the severity of the disease of the recipient.

The patient subcutaneously, in the pre-formed capsule has entered the culture of the cells in the pancreas of newborn rabbits. After 7 days, the patient had improvement in General condition, reduced sense of thirst and dry mouth, decreased numbers of blood pressure to 140/90 mm RT.article After 15 days the patient's condition has reduced the need for exogenous insulin 30 units to 18 units (blood and urine). After 30 days, the need for exogenous insulin decreased to 12 IU/day, and by the end of the 2nd month up to 4 meals/day.

The patient is observed for 12 months. Clinical manifestations of General anti-diabetic drugs with mandatory adherence to diabetic diet and monitoring blood glucose, in the urine and glycated hemoglobin.

Example 2. Patient K., 52 years old. Insulin-dependent diabetes mellitus diagnosed 18 years due to severe stressful situation. The nature of the disease was first unstable heavy. Doses of exogenous insulin reached 70 IU/day. In recent years, the nature of the disease has stabilized, but the deterioration occurred after stressful situations and errors in diet.

In the last three years was noted deterioration of the vessels of the lower extremities, decreased libido, decrease in erection quality and sexual intercourse. Diagnosed with diabetic angiopathy of the lower extremities and the penis. The need for exogenous insulin over the last year from 20 IU/day up to 40 IU/day.

In the pre-formed capsule subcutaneously to the patient was administered a culture of pancreatic cells 14 day piglets. Two weeks later the patient noted improvement in General condition. One month later the need for exogenous insulin decreased to 12 IU/day. After 2 months - up to 6 units/day. 4 months after transplantation the patient was transferred to oral antidiabetic drugs. Normal sexual life of the patient significantly improved the tov, data of additional methods of investigation (blood, urine) allow you to talk about high efficiency of this method of treatment of diabetes, which leads to a significant dose reduction of patient use of exogenous insulin, and in some cases, and in the refusal of insulin therapy. The method does not require immunosupresivny therapy, reduces the risk of secondary complications of diabetes: retino-, neuro-, nephropathy, can significantly improve the quality of life of patients. therapeutic effect lasts, as a rule, from 10 to 20 months depending on the severity of the disease. The number of transplanted cells is also determined by the severity of diabetes, in particular the amount of sick of exogenous insulin.

A method of treating diabetes by transplanting cells of the pancreas, characterized in that the recipient previously administered polyacrylamide gel, followed by transplantation into him-cells of the pancreas.

 

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