The tool for an effective immune response in cattle to erythrocyte antigens

 

(57) Abstract:

The invention relates to the field of animal husbandry. The tool includes an emulsifier and oil-based. As the emulsifier, the tool contains an antimicrobial stimulator Dorogov fraction - 2, and as oil fundamentals - oil solution of fat-soluble vitamins A, D, E, F. the Tool further comprises calcium gluconate and glycerol calcium in the following ratio of initial components, wt.%: glycyrrhizinate calcium 0,050 - 0,100, calcium gluconate 0,020 - 0,050, ASD fraction-2 100 ml oil solution of fat-soluble vitamins to 1000 ml with activity AND 50000 IU/ml, activity D 25000 IU/ml, content of E 20 mg/ml, the content of F 5 mg/ml Tool allows you to increase by 20 - 30 % of production EA-tiirough sera, does not give in the application of side effects, 1 C.p. f-crystals.

The invention relates to immunogenetic methods in animal husbandry, in particular to the means of enhancing the immune response in cattle.

Known means for this purpose various immunomodulators (cultures of yeasts, adjuvant's adjuvant, stable lymph, and so on). However, such tools do not guarantee an effective immune is more close analog in the composition and physiological action is a tool including emulsifier (organic copolymer) and oil-based (see the patent of Russian Federation N 2108111, class A 61 K 39/39, 1998), which is accepted by us for the prototype.

However, the known tool developed for medical purposes, involves a complex and inaccessible components and there is absolutely no information about its effects on the immune response, for example for the generation of antibodies to some genetic markers in cattle and other farm animals.

The purpose of the invention to provide a means of improving the production of antibodies that reduce the manifestation of side effects during treatment with the drug, reducing time and costs for processing animal upon receipt of sera for immunogenetic testing of cattle.

This objective is achieved in that, for obtaining an effective immune response upon immunization of recipients of donor herds of cattle erythrocyte antigens (whole blood donors) proposed to use the immunomodulatory agent, which includes:

100 ml of antimicrobial stimulator Dorogov fraction-2 (ASD f-2), which is a product siloti, their amides and ammonium salts, kalinovye esters of carboxylic acids, choline, primary and secondary amines, sulfhydryl groups), and inorganic nitrogen compounds (ammonium salts of carbonic, acetic acid and others) and water up to 75%, anti - inflammatory drugs-calcium gluconate and glycerol calcium in the ratio, wt.%: calcium gluconate 0,020-0,050, glycerol calcium 0,050-0,100, in oil solution of vitamins A, D, E, F, (tetravit), with the activity of A - 50000 IU/ml, D - 25000 IU/ml, and vitamin E 20 mg/ml, F - 5 mg/ml

For immunization prepare a remedy by mixing at room temperature, 5 ml of 10% solution of calcium gluconate and 0.5 g of powder glycerol calcium with 100 ml of antimicrobial stimulator Dorogov fraction-2 and add the above tetravit (oil solution of vitamins A, D, E, F) up to 1000 ml.

The resulting tool is administered once in a dose of 0.025 ml per 1 kg body weight intramuscularly before immunization recipients of whole blood donors.

The biological activity of the proposed tool was tested on 80 bulls Yaroslavl breed 8-9 months of age, live weight 230-240 kg Of the animal formed 4 groups of 20 bulls each. Animals first is was heteropoly 15 ml of levamisole each animal in the same way. The third group was injected advent's adjuvant at a dose of 20 ml per animal. All groups of animals were immunized intramuscularly whole blood donors at a dose of 20 ml each animal.

The control were the recipients, immunization, which were the only whole blood intramuscularly at a dose of 20 ml per animal.

The results of the experiments showed that under the impact of the proposed substances the number of reactive animals was higher (70%) than in groups where it was used advent's adjuvant (60%), levamisole (60%) and whole blood (40%).

The titer of the resulting antibodies, depending on the applied immunomodulator, had significant differences. Most animals with antibody titer of 1: 32 and higher were observed (60%) in the group using the proposed tool. This group was not observed symptoms of side effects like swelling, pain at the injection site of the stimulator.

In the group of animals using adjuvant's adjuvant percentage of reactive animals with titers of 1:32 and higher than 50%, in the group using levamisole - 40%, in the group with whole blood - 30%.

The application of the proposed means increases the number of animals that produce antibodies with a higher titer, the negative signs means for the production of antibodies not detected. Modulating effect in the immunization of animals due to possible neurotropic holino-mimetic effect on the Central and autonomic nervous system, and increased activity of tissue enzymes involved in the active transport of ions and nutrients through cell membranes. The retention and use of available funds not later than 6 months from the date of manufacture.

1. The tool for an effective immune response in cattle to red blood cell antigens, comprising the emulsifier and oil-based, characterized in that the emulsifier contains antimicrobial stimulator Dorogov faction 2, oil fundamentals - complex fat-soluble vitamins a, D, E, F, and further includes a salt gluconate and glycerol calcium, while these components are contained in the following, wt%:

Glycyrrhizinate calcium - 0,050 - 0,100

Calcium gluconate - 0,020 - 0,050

ASD faction 2 - 100 ml

Oil solution of fat-soluble vitamins - Up to 1000 ml

2. Means under item 1, characterized in that the oil solution of fat-soluble vitamins include vitamins:

activity

A - 50000 IU,

D - 25000 IU

and content

E - 20 m

 

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