The saccharomyces cerevisiae strain to obtain alcohol and biomass of baker's yeast

 

(57) Abstract:

The invention relates to microbiological, alcohol and yeast industry. Acselectionsetall strain Saccharomyces cerevisiae Y2283 for alcohol production and biomass of Baker's yeast from starch-containing raw materials. The strain is characterized by a high glucoamylase activity ( 1-1,25 u/ml), which eliminates the use of glucoamylase in the process of saccharification of starch-containing raw materials for alcohol production and biomass of Baker's yeast.

The invention relates to microbiological, alcohol and yeast industry.

At the present time for the production of alcohol from starch-containing raw materials used thermotolerant strain of the yeast Saccharomyces cerevisiae Y1986 with glucoamylase activity. The use of this strain of yeast is not enough provides reduction saharaui the funds required for the hydrolysis of plant starch in ethanol production (1).

The disadvantage of this strain is ineffective production of endogenous glucoamylase.

The purpose of this invention is to provide a strain thermotolerant yeast with high glucoamylase activity, JV is required for saccharification of starch, used in the production of ethanol and biomass of Baker's yeast.

The Saccharomyces cerevisiae strain Y2283 obtained by removing carbon catabolite repression of the gene of glucoamylase. Conducted selection of clones for maximum production of the enzyme glucoamylase and excluded making costly component - glucoamylase for saccharification of starch for alcohol production and biomass of Baker's yeast.

The strain deposited at the all-Union collection of industrial microorganisms (VKPM) under number Y2283.

The strain is characterized by the following cultural-morphological and physiological-biochemical characteristics.

Vegetative cells of a two-day culture grown on solid nutrient medium with 2% starch as the sole carbon source, have an elongated, rarely rounded shape, the size of the cells of 3.0 x 6.5 μm, a homogeneous protoplasm, reproduction by budding.

When grown on solid medium containing yeast extract and peptone, (YEP) if 30oC after 72 h of growth of the colony are as follows:

1) on the environment YEP with glucose colonies white with a smooth edge, shiny surface, a conical profile, creamy Kohanim profile and krupeninoy consistency;

3) on the environment YEP with starch and galactose colony of white color with a matte finish, smooth edge, a convex profile and krupeninoy consistency.

Growth in liquid medium.

On malt wort at the 35oC during the first 24 h of cultivation - liquid cloudy, white precipitate, not crumpled, parietal film is not formed, the smell of ethanol.

On the environment YEP, containing 3% starch, within 20 h of cultivation - turbid liquid, characteristic odor of yeast.

Facultative aerobe. The temperature of the growth - 23 - 37oC (optimum - 32oC). The pH values under cultivation - 3,8 - 6,7 (optimum - 5,0).

Assimilation of carbon sources: sprayway glucose, galactose, fructose, maltose, sucrose, dextrin, starch.

Assimilation of nitrogen sources: absorbs amino acids, urea, ammonium sulphate, ammonium nitrate.

Distinctive features: under cultivation on solid medium YEP starch (2%) and galactose (2%) around the colonies formed a clear zone of enlightenment starch after incubation cups at +4oC for 24 h

The Saccharomyces cerevisiae strain Y2283 non-pathogenic.

The strain is stored on a rich agar medium with starch and the positive environment is unfiltered grain rye mash with a concentration of solids 16%, which is made from grinding rye, followed by cooking at 1.5 ATM for 1.5 hours Wort Osharova when 56oC for 60 minutes, adding the enzyme aminocoumarin at the rate of 0.5% per 1 g of the conventional starch. The glucoamylase is added. In saharienne thus rye mash seeded with cells of strains Y1986 and Y2283 in title 1 of 107and conduct the fermentation at 37oC for 72 h During fermentation occurs dosageiwant starch rye mash the glucoamylase produced by yeast cells. Glucoamylase activity after 60 hours in strain Y2283 is 1-1,25 u/ml, while in strain Y1986 only 0.2 to 0.25 u/ml (2). After 72 hours the content of soluble non-fermented carbohydrate is: strain Y1986 - 2.2 g/100 ml, Y2283 - 0.7 g/100 ml

Example 2. A nutrient medium is a liquid medium YEP, containing 3% starch. Enzymes do not add. Cells of strains Y2283 and Y1986 seeded in title 5 of 106and grown in the fermenter at 30oC; pH 4,8; pO240%. After 20 hours after the start of culturing dilutions of cells of strain Y2283 is 4 to 108and in strain Y1986 significantly lower, ranging from 5 to 107cells in 1 ml of medium. Strain Y2283 up to 90% starch with a conversion rate of 45%.

T and biomass bakery yeast, starch-containing raw materials without reducing their output, if the production of biomass even increases it.

Sources of information

1. RF patent N 2001097 C1, C 12 N 1/16, 1993.

2. The enzyme preparations. GOST 20264.4-74. Publishing house of standards, 1975.

The Saccharomyces cerevisiae strain VKPM Y 2283 to obtain alcohol and biomass of Baker's yeast.

 

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