Antiherpetic agent

 

(57) Abstract:

The invention relates to medicine. The proposed use of aradena as antiherpetic funds. The drug exhibits antiviral activity. table 2.

The invention relates to the field of medicine and, in particular, ophthalmology.

Currently in ophthalmic practice as antiviral agents for the treatment of eye diseases, caused by herpes simplex virus, found the use of the following medications: 0.1% of R-R GO 3% ointment Vicurban, Halpin, Bonafton, Interferon, Zovirax [Y. F., Michuk Viral diseases of the eye" - M "Medicine" 1981, 272 S.].

However, the use of the above drugs are not always effective. This is due to many reasons - low-solubility drugs, inability to provide therapeutic concentrations at the site of reproduction of the virus, toxic effect of chemotherapeutic agents on membranes of the eyes, as well as the development of resistance to all the prescribed drugs. Therefore, for the successful therapy of diseases of the eye caused by the herpes simplex virus, need for new antiviral drugs and medications that improve trophism and regeneration by the VA is a 5-iodine-2 deoxyuridine (IMU), of 0.1% eye drops ARE widely used in ophthalmic practice for the treatment of eye diseases, caused by herpes simplex virus, and in addition, ARE still the main drug - a "marker" in the experimental evaluation of new anti-herpes funds (Y. F., Michuk "Eye infections" - Russian Honey. J., 1999, T. 7, N 1, 16-19].

Known drug atadan belonging to the group of stimulants regenerative processes in tissues, which are based on the activation processes of biosynthesis of nucleic acids. Atadan in medical practice for the treatment of thermal and radiation injuries of the skin, with long-term non-healing wounds in the form of a powder and 1% injection solution [Etagen: Avenue M, cbti Honey. prom., 1985 - 18 C.].

We have previously shown that the drug atadan in the form of a 0.5% aqueous solution as a reparative drug is effective in the treatment of ocular viral diseases involving destruction (ulcerative lesions) and trophic disorders of the cornea.

It is established that the combination as a specific anti-herpes drug instillation antiviral drug GO and as reparative means a 0.5% aqueous solution of aradena, to the cure [Patent N 2122844 from 17.06.96 "a Method for the treatment of herpetic keratitis with ulceration" Y. F. Michuk and others].

However, further experimental studies showed that the drug atadan independently exhibits antiviral activity against herpes simplex virus.

Antiherpetic activity of this drug in the existing literature is not described.

The essence of the invention lies in the fact that the drug reparative actions atadan exhibits antiviral activity against herpes simplex virus in the in vitro system to model cell culture and in vivo in experimental herpetic keratitis rabbits ' eyes.

Example 1. Antiherpetic activity of aradena in vitro.

The test was performed on the tissue culture of fibroblasts of chicken embryo, which was prepared by the standard technique.

Cell suspension at a concentration of 1 million/CL in ml contributed to 0.2 ml in each well of 96-aceing culture plates. Cells were incubated 48 hours in an incubator at 37oC (85% medium 199+15% bovine serum, without preservative). Then the monolayers were washed once with Hanks solution and infected with herpes simplex virus strain "1-C" type 1 consecutive 10-multiple dilutions. The virus and made the test drug in the following concentrations: 1000; 100; 10; 1,0; 0,10; 0,01 g/ml of cell Culture was placed in a thermostat at 37oC. the Results were considered by the cytopathic effect after 48 and 72 h

Served as control monolayers of cells treated only with the virus without drug (control virus), and monolayers of cells treated with the tested concentrations of the drug without virus (control toxicity).

The results are given in table 1.

Thus, it was established that atadan has antiviral activity in vitro against herpes simplex virus by inhibiting viral replication at concentrations 100,0-0.1 g/ml And it is shown that at a concentration of 100 g/ml atadan reduces the infectious titer of 3 lg 48 h after infection and 2.5 lg 72 hours of the Toxic effect of the drug on the cells at these concentrations is not marked.

Example 2. Antiherpetic activity of aradena in vivo. Study of antiviral activity in vivo has been studied in experimental models of ophthalmic herpes rabbits. In the experience took 10 rabbits (20 eyes). Reproduction of experimental models of ophthalmic herpes was carried out according to the usual method, by scarification of the cornea and subsequent rubbing virusanderica when due according to the clinical criteria of the group. Experimental group received instillation of 0.5% solution of aradena 3-4 times a day; the control group of animals received placebo. Treatment was continued until complete healing of the rabbits in the experimental group. About the effectiveness of the drug was assessed in terms of epithelialization of the cornea, as well as in terms of cure.

The research results are summarized in table 2.

As can be seen from the presented results, the instillation of 0.5% aqueous solution of aradena provides a pronounced therapeutic effect, accelerate the process of epitomization of the cornea, to accelerate the recovery of animals in a significantly short period of time (p<0.05), and also noted a higher percentage of recovered animals in the experimental group compared with the control (p<0,05) due to its anti-herpes activity.

The use of aradena as antiherpetic funds.

 

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