The method of obtaining funds with anti opisthorchosis action

 

(57) Abstract:

The invention relates to medicine. Shredded aspen bark is extracted with water at 70C mixing ratio of raw materials : water of from 1 : 8 to 1 : 10, infused over 4-6 hours, drained, defend extract within 2-4 h, concentrated in vacuo at 30-50C to 60 wt.%, the residue is extracted with 45% ethyl alcohol in a ratio of components 1 : 1 for 4-6 hours, drained, combine the water and alcoholic extracts, followed by evaporation and drying in vacuum. The invention improves the yield and specific activity funds to improve organoleptic properties.

The invention relates to medicine and can be used to obtain medicines from plant material with anti opisthorchosis action.

Known anthelmintic agent "Comelit", which is a product of the production of oil from pumpkin seeds (U.S. Pat. N 2113474, A 61 K 35/78, B N 20, 1998). However, this tool is not effective against opisthorchis.

Known choleretic (U.S. Pat. N 2126687, A 61 K 35/78, BI N 6, 1999) and antiulcer (U.S. Pat. N 2124899, A 61 K 35/78, BI N 2, 1999; U.S. Pat. N 2086251, A 61 K 35/78, BI N 22, 1997) funds from the bark of aspen.

Closest to the accom is BI N 27, 1996), which consists in the extraction of the bark of aspen repercolation method 70% aqueous alcohol. However, this tool has a very bitter taste, poor yield of the target product. In addition, its specific activity is insufficient.

The objective of the invention is the improvement of the yield of the target product, the increase of specific activity funds, improved organoleptic characteristics.

The technical result is achieved by the fact that the aspen bark, crushed to sizes less than 1 mm, pour water at a temperature of 70oC in the ratio of raw materials: water of from 1:8 to 1:10. Insisting lead for 4-6 hours, then the extract is poured into another container to advocate for 2-4 hours, concentrated in vacuo at a temperature of 30-50oC to a concentration of 60% wt. Pressed bark of aspen fill in 45% ethanol and leave for 4-6 hours. The extract is separated, connect it with the aqueous extract is then evaporated and dried in vacuum. Get the dry powder yellow-brown color.

Examples

Example 1. 10 kg of the bark of aspen (Populus tremula L) is crushed to sizes less than 1 mm, pour 80 l of water and leave in the 70oC for infusion for 4 hours. Then the bark of aspen press, the extract is poured into the other is elewaut 45% ethyl alcohol at a mass ratio of 1:1 and insist 4 hours. Alcohol extract is separated, combined with water, evaporated and dried in vacuum. The yield of dry product 1.6 kg (16% wt.).

Example 2. 10 kg of the bark of aspen (Populus tremula L) is crushed to sizes less than 1 mm, pour 100 litres of water and leave in the 70oC for infusion for 6 hours. Then the bark of aspen press, extract assert within 4 hours, concentrated in vacuo to 60% at a temperature of 50oC. Wrung the bark of aspen fill 45% ethyl alcohol at a mass ratio of 1:1 and leave for 6 hours. Alcohol extract is separated, combined with water, evaporated and dried in vacuum. The yield of dry product 2 kg (20% wt.).

Clinical trials of dry aspen bark extract, obtained by the proposed method and the method prototype, conducted on 26 patients with chronic opisthorchiasis (9 men and 17 women) aged 24-56 years with duration of the disease from 2 to 18 years. The diagnosis of chronic opisthorchiasis verify data microscopy of duodenal contents and feces.

Individuals were divided into 2 groups, identical by gender and age, depending on the specific therapy. Aspen bark extract is administered in the day, in 3 divided doses with an interval of 4 hours according to the following scheme: to the analysis of the effectiveness of the proposed drug (1) drug, obtained by the method prototype (2), all patients underwent a comprehensive clinical and laboratory research in dynamics before specific treatment, after 1 and 3 months after treatment using standard methods of laboratory diagnostics. The conclusion regarding complete parasitological recovery was based on the negative results of three aprovechamiento studies and one-time microscopy of bile.

All surveyed identified cholangiohepatitis, which was characterized by manifestations of pain, dyspeptic and asteno vegetative syndromes; the intensity of infection is less than 100 eggs of opisthorchis in 1 g of feces.

1 month after deworming both products (1 and 2) there was a significant decrease in the frequency and severity of the main clinical symptoms of the disease. Parasitological recovery occurred in 71,4% of those receiving the drug 1 and 58.3% of the drug 2. The results of the survey 3 months later testified exemption from opisthorchosis invasion 85,7% of the 14 individuals who were prescribed the drug, obtained by the proposed method (1) and in 66.7% of the 12 persons using the prototype method.

Thus, the proposed JV is less bitter taste, with a large 1.2-1.5 times the output compared to the prototype.

The method of obtaining funds with anti opisthorchosis action, by extraction of the bark of aspen aqueous solution of ethyl alcohol, characterized in that the crushed bark of aspen pre-extracted with water at 70oC in the ratio of components raw materials : water of from 1 : 8 to 1 : 10, infused for 4 to 6 hours, drained, defend extract for 2 to 4 h, concentrated in vacuo at 30 - 50oC to 60 wt.%, the residue is extracted with 45% ethyl alcohol in a ratio of components 1 : 1 for 4 - 6 hours, drained, combine the water and alcoholic extracts, followed by evaporation and drying in vacuum.

 

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