Method of preparation of ointments wound healing, burns and anti-inflammatory action for animals

 

(57) Abstract:

The invention relates to veterinary medicine. The method involves pre-heating the petrolatum and lanolin, fractional flow of all components of the mixture with thorough mixing each portion, and the components are taken in the following ratio, wt.%: oil fir - 5-10, lanolin - 0,2-5, petrolatum else. The ointment is highly effective with a long shelf life, no side effects.

The invention relates to the field of veterinary medicine, namely to a method for producing a medicinal product based on vegetable raw materials used for the treatment of wounds, burns, eczema, dermatitis, burstow, injuries, traumatic tissue damage.

Known anti-inflammatory ointment "Picton" including pine resin, natural bee wax, chalk, fat basis and method of its manufacture (see patent RU 2097056 C1. 27.11.97).

However, this method is time-consuming, pine resin before mixing with other components must be subjected to mechanical cleaning, etc.

Known ointment for the treatment of skin diseases, containing a mixture of pine oil and aqueous extract of Siberian fir from fir LAPC the
However, the method of manufacturing this ointment is time-consuming, as it involves obtaining a mixture of pine oil and aqueous extract of Siberian fir, besides the content of active components in the extract of Siberian fir - waste production of fir oil - unstable, which affects the quality of the product, and heating of vaseline to a temperature of 35-40oC does not allow for microbiological purity of the product.

High lanolin content in the grease increases its value and makes it unsuitable for use in veterinary medicine.

The aim of the invention is to develop a low-overhead method of making a low cost effective ointment for the treatment of wounds, burns, eczema, dermatitis, burstow, injuries, and traumatic damage to the tissue in animals.

This objective is achieved in that the petrolatum is heated to a temperature of 65-75oC, maintained at this temperature for 50 min, lanolin pre-heated to a temperature of 60-65oC and incubated for 40 min, then separated 2/3 hours vaseline, stirred for 10 min, and then fractionally serves lanolin and the resulting mixture is stirred for 10-15 minutes Then serve the remaining roboy oil flow fir 10 min mixing each portion. The mixture was incubated at T=55-60oC for 1 h, then cooled to T=45-40oC, and packing is carried out at T=35-40oC, the components are taken in the following proportions (wt.%): oil fir - 5-10, lanolin - 0,2-5, petrolatum else.

The drug is a homogeneous pasty mass of light yellow color with a smooth consistency.

The fir oil meets the requirements of OST 13-221-86, as fillers use anhydrous lanolin to GF, X, p 373 or FS 42-25-20-88 and vaseline medical GOST 3582-84, or veterinary GOST 10037-84.

Lanolin and vaseline reduce sensitizing properties of pine oil and improve ergonomics.

The resulting ointment when applied to the skin of animals diffuses through the steel and sweat glands, which is gradually absorbed, causing expansion of vessels, improves blood circulation, accelerates the resorption of products for inflammation, growth of granulation tissue, epithelialization of wounds. The drug is applied in a thin layer on a clean white cloth or gauze, which previously covered the affected area of the body, then wipe with a drug covered with a bandage or special glue. The ligation PR is, gently rubbing it into the fabric.

When injuries, mastitis impose warming bandages.

Application of ointment no side effects and does not cause complications in animals.

Contraindications for the drug is not installed.

Example N 1

Vaseline, which is supplied in 200 litre drums, put on steam battery, after the vaseline will soften, his pump serves to measure where the measure required quantity. Heated vaseline to a temperature of 65-75oC. Maintaining at a predetermined temperature for 50 minutes This temperature required to create the microbiological purity of vaseline.

The lanolin in 200-liter barrels also pre-heated on a steam-powered. The softened lanolin is pumped into the measuring device. Create temperature 60-65oC for 40 min to obtain microbiological purity of this component.

On the scales weigh the required amount of pine oil.

Then pumped 2/3 hours of vaseline in the reactor, supported temperature 65-70oC, stir for 10 min, then added in small portions lanolin (for the a, the temperature of the mass withstand 65-70oC for 1 h after a specified time served weighted number of fir oil (filing of fir oil fraction), after submitting each 1/10 including pine oil produced 10 - minute mixing (i.e. download pine oil 10-fold). This dosage allows you to create the homogeneity of the composition.

After downloading the entire fir oil this mass is maintained at a temperature of 55-60oC for 1 h, then the mass is cooled to a temperature 45-40oC. After which the laboratory selects ointment for analysis. In case of positive conclusion of the laboratory quality is packing the ointment at a temperature of 35-40oC.

The components of the mixture taken in the following proportions (wt.%):

Oil fir - 8

Lanolin - 2,6

Vaseline - Rest

As a result of researches it is established: ointment pale yellow of various shades, enjoys the smell of pine oil, the presence of visible particles is not more than 1 sample 4, the mass fraction of barcelonetta (DV-fir oil - 2,80-3,12), microbiological purity within the GFATM requirements.

Retention ointment - 2 years.

Example N 2

The study of skin-F. each rabbit was not called within 24-48 h of any visible changes. Irritation by pricking with a needle reaction was adequate strength of the shot.

However, when applied to white mice these ointments mow the area of the coat, in places where the skin was scarification, we noted the redness of the skin, in places slits noted the increased blood flow, as the peripheral vessels of the skin were filled directly proportional to the amount of the ointment. Reaction to the irritation in the first 3 h was abrupt and after three hours there is a weakening of redness of the skin with large quantities of ointment and ointment containing 0.2% lanolin, three hours did not cause further changes of the skin.

Three rabbits molten ointment instilled in the left eye in the amount of 0.2, 0.4 and 0.6, When viewed from the left eye through 3, 6, 9, 18, 24, 48 and 64 h it was found that the conjunctiva of the left eye in rabbits, red in proportion to the number of applied ointments. It should be noted that this did not cause redness swelling neither the lower nor the upper eyelid of the left eye. After 24 hours the redness has decreased, but it should be noted that the blood vessels of the conjunctiva of the left eye injected and it lasted for 36-48 hours depending on nonsenior N 3

The effect of the ointment on the wound process

After applying non-sterile scalpel wounds two sheep in the lumbar region, a day after the wound and treating the skin by vystrogana coat, began to treat wounds. Wounds in both sheep per day has undergone a dramatic inflammatory process. One sheep (females) in the "pocket" between the skin and subcutaneous fascia streamed blood has been putrid decay.

The first sheep (male) put ointment on all areas of the wound, the second sheep (female) laid additionally ointment in the "pocket". After four days the condition of the wounds in both sheep has improved, the length of the wound decreased wound healing proceeded by granulation. The resulting granulation of wounds in sheep swollen, crownpointe. Edges of wounds in sheep slightly swollen, reddened; palpation of the wound edges is felt hypersensitivity wound edges. The healing process for this period of time passes in a satisfactory condition. The ointment was applied to the plots wounds once per day.

In the home economy citizen Anisimov A. C. was treated wounds in the field of ungulate joint cows at the age of 7 years. Grazing contents cakes. When viewed from the rear extremity detected lameness, swelling in the area of the Corolla and mercapital cracks and damage soft tissue palpation was found to have pain, after a bloody fluid with presence of putrefactive processes.

When treatment was applied bandage with ointment and a tight bandage. Two days after the bandage is removed, the swelling of the right of the Corolla and mercapital cracks disappeared, slightly there is a slight lameness on the right rear limb.

Example N 4

Study of acute toxicity ointment

Sredneseriynoe dose ointment containing 0.2% lanolin, when administered orally to white mice is 82173,2 Ál/kg of body weight, which gives rise to attribute this drug to IV class of hazard (GOST 12.007-76).

Example N 5

Assessment of local skin-irritating ointment

Evaluation of local skin-irritating ointment conducted on rabbits by a single exposure by the open method on prepared skin with 6-hour exposure. The average weight of rabbits was 2-3 kg the results of the research indicate that skin-irritating ointment is not.

Studying irritating properties mnom direction were performed on white mice, rats, rabbits and Guinea pigs. The average body weight of animals was B. mice, 25-30 g, rats 180-220, To experience these animals were kept on a fixed diet (diet) during the week. For the experiment on the back of each animal's shaved skin area size: mouse 1 x 1 cm, rats 2 x 2 cm were Removed all wool and a day rubbed drug at the rate of 0.2 g/kg animal body weight. The drug was tested on 10 mice, 5 rats. Control groups of animals (10 mice and 5 rats) rubbed ointment Vishnevskogo. A visual assessment of the skin of experimental animals was made every day. Take into account the behavior of animals, the condition of skin in place of rubbing of the drug. Studies have shown that 1-fold and 7-fold rubbing ointment (1 per day) in the dose did not cause changes in the behavior of animals, did not lead them to ruin. Not marked redness, scratching thickening of the skin fold at the site of the drug even after 7-fold application.

Example N 6

Allergenic properties of ointments

The study was conducted on 10 white Mature Guinea pigs.

The multiplicity of applications for the sensitization of animals - 1, dexterously - 10, exposure 4 hours a day. Square test epicutaneously 24, 48, 72 and 96 h using a scale Alekseeva O., Control (5 Guinea pigs) - distilled water. The lack of positive skin reactions to the drug indicates that the allergenic activity of the ointment does not possess.

Example N 7

Study of the effects of the ointment on the body of the sheep

The effect of the ointment on the clinical status and biochemical status of the blood of experimental animals was studied in sheep. In experimental animals (3 sheep) was vestigal and shaved in the area of cereal plot size of 15 x 15 cm Within 5 days on this stretch was applied 30 g ointment. Determination of hematological parameters was carried out by standard methods and included determination of HB, leukocytes, erythrocytes. The study of biochemical status consisted of the determination of total protein, glucose, LDH activity and MDY. Biochemical parameters were determined spectrophotometrically according to standardized methods (Menchov and others 1978). Blood from sheep took to experience 1.5, 10 day experiment. Throughout the experiment, the breath, the pulse of the animals did not change.

Appetite, natural origin were normal.

The data indicate that none of the studied parameters did not change during the experience. This fact with the N 8

The study of reparative properties of the ointment.

Experiments on wounds was carried out on 9 rabbits. When applying the RAS in the area of the outer surface of the right thigh certain area was vestigal and shaved hair. In the center section of scissors cut out flap with a diameter of about 1 cm Wound deepened 0.5 cm without complying with the rules of asepsis. The sizes of the wounds were determined using polyethylene film (method cephalogram), then counting the size of the wound surface (within 10 days). One day after injury in rabbits was observed depression, decreased appetite, increased local temperature colornews surface. Wounds to the level of the edges filled with blood clots in the lower right corner to reveal the accumulation of a small amount of clear fluid.

After injury to the skin blurring the edges of the wound was performed after 2-5 hours a wound Treatment was performed daily for 10 days. As a result of our research, it was found that on the second day the animals were no dead tissue, phase hydration and dehydration are weak. Therefore, wound healing occurred in a relatively short time, without signs of suppuration, on the background of weakly expressed tramarital a scab fibrin dark color, in every part of the accumulation of clear fluid. The muscles examined in the presence of exudate green. In this phase of wound healing is observed the increase of the inflammatory response with the development of exclusion fibrinogenesis mass. After 4 days in animals treated with ointment, appeared granulation roller (around the lesion), which was gradually extended, gradually developed the epithelization process. Full regeneration occurred after 12-14 days. In the control group (without treatment) the healing process continued 22-25 days.

Thus, the proposed method allows to obtain highly effective ointment with a long shelf life, which has no side effects, does not cause complications in animals and has no contraindications.

Method of preparation of ointments wound healing, burns and anti-inflammatory action for animals, comprising mixing the fir oil, lanolin and vaseline and packing of the drug, wherein the petrolatum is heated to a temperature of 65 - 75oC and maintained at this temperature for 50 min, and lanolin pre-heated to a temperature of 60 - 65oC and incubated for 10 m is fractional, the resulting mixture is stirred for 10 to 15 minutes, and then serve the remaining 1/3 of vaseline, the resulting mass is maintained at a temperature of 65 - 70oC for 1 h, then carry out a 10-cratonal fractional flow of oil fir with a 10-minute mixing each portion, after which the mixture was incubated at a temperature of 55 - 60oC for 1 h and cooled to a temperature of 45 - 40oC, and packing of the drug is carried out at a temperature of 35 - 40oC, the components are taken in the following ratio, wt.%:

Oil fir - 5 - 10

Lanolin is 0.2 - 5

Vaseline - Rest

 

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SUBSTANCE: invention relates to the field in development of an agent of vegetable origin used for improvement of the functional state and prophylaxis of organic alterations in cardiovascular system, in particular, atherosclerosis. The biologically active supplement used for prophylaxis of cardiovascular diseases, in particular, atherosclerosis comprises clover dry extract, haw-thorn flowers powder, ascorbic and nicotinic acids, and accessory substances taken in the definite ratio. The biologically active supplement is made in the form of tablet or capsule. No adverse responses observed in intake of the supplement. The blood cholesterol level was reduced to the normal value in 68% of patients and reducing morbidity with acute respiratory-viral infections has been noticed. In patients with ischemic heart disease with cholesterolemia the improvement of the state is noted on the background of conventional therapy: the moderate reducing the arterial pressure value in its elevation, improved in the state of health, enhanced working capacity, reduced retrosternal pains, ear noise, vertigo, headache.

EFFECT: valuable medicinal properties of supplement.

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