Biotechnological method for integrated processing of waste palm oil production
(57) Abstract:The invention relates to a method of processing waste in palm oil production. Solid waste is subjected to acid hydrolysis. Hydrolyzed solid waste is mixed with liquid waste. Seeding is performed by the biomass of the bacterial strain Acinetobacter baumanni M-1. Conduct liquid-phase fermentation at pH of 6.5-7.5 and a temperature of 35-45°C. the Obtained microbial suspension sephirot. Carry out the purification of the liquid flow. Condensed biomass is subjected to heat treatment at 90°C for 1 h Then dried and receive feed protein. Solid waste and solid fraction formed after the liquid-phase fermentation is subjected to solid-phase fermentation at pH of 6.5-7.5 and a temperature of 35-45°C. Receive the finished product - compost. The method provides environmental cleanliness palm oil production and allows to obtain commercial products in the form of protein feed and compost. 4 C.p. f-crystals. The invention relates to the field of biotechnology and ecology.The closest in content to the invention is English patent "Method of wastewater treatment process of obtaining palm oil (GB 1601350, 28.10.81), which describes sposobnost activated sludge.The difference of the invention from similar is that complex biological utilization is subjected as the waste water generated during the production of palm oil, and solid waste significant amounts accumulated in the processing of palm fruit. Thus attain a twofold purpose: a) environmental - wastewater treatment from harmful impurities; b) economic - receive valuable high-protein feed for livestock and production of highly efficient organic fertilizer (compost). To reach this aim by complex methods of liquid-phase and solid state fermentation using specially selected bacterial culture. Liquid and solid waste mix to the dry matter content of 10% and subjected to liquid-phase fermentation using a strain of bacteria Acinetobacter baumanni M-1.However, solid waste previously subjected to acid hydrolysis using acid instead of liquid waste from palm oil production, having a low pH value. Formed during fermentation of the biomass is separated from the solids and then concentrated and dried. You get a finished product - cormocephalus fermentation, mixed with solid waste (conditioned and unconditioned) and subjected to solid-phase fermentation together with excess activated sludge ponds aeration tanks, manure or litter. You get a finished product-compost. Purification released after liquid-phase fermentation of wastewater is carried out in aerated ponds up to required parameters.The description of technology of complex processing of waste palm oil production
Liquid-phase fermentation.Liquid-phase fermentation of the mixture of liquid and solid waste carried out in the apparatus with stirring and aeration in batch and continuous modes of cultivation adding mineral sources of nitrogen and phosphorus, pH 6.5 - 7.5, temperature 35 - 45oC. however, solid waste is subjected to a preliminary acid hydrolysis at a temperature of 90oC for one hour. Instead of the acid used for hydrolysis liquid waste from palm oil production, having a pH of 3.5 - 4.5. Hydrolyzed solid waste is mixed with liquid waste to the dry matter content of 10%. Seeding is performed by the biomass of the bacterial strain Acinetobacter baumanni M-1.Characteristics of the strain of bacteria Acinetobacter baumanni M-1.Cells Merom 1,1-1,h,0-1,5 μm, single and dual, fixed, nesporoobrazuth, gram. Oxidatively, catalogoplantillas.Colony one-, two-day culture on meat-peptone agar, globose, size of 1-2 mm, with smooth edge, slightly convex, smooth, shiny, non-pigmented. Aerobe. Chemoorganotrophs. Assimilates: glucose, sucrose, fructose, maltose, ornithine, leucine, 2,3-butyleneglycol, malate, tyrosine, aspartate, lactate, triglyceride, urea, aspargine, ammonium sulfate, monoammonium phosphate, potassium nitrate. Not assimilates: D-xylose, arabinose, -alanine. Not hydrolyzes gelatin. Restores nitrates. Urease is not formed. Has aminocellulose activity. Temperature growth 25-48oC. non-Pathogenic, non-toxic.The suspension obtained after liquid-phase fermentation, divided into the drum sit on the microbial suspension and a solid fraction. Microbial suspension sephirot and condensed biomass is subjected to heat treatment at 90oC for one hour to "obuvki live cell culture-producer and dried in the spray dryer with inlet temperature of 500-600oC. thus receive the finished product - feed protein having the following characteristics:
the lipids of 2.5-3%,
the content of crude protein not less than 50%.The liquid stream after separation is subjected to a purification apparatus when aurelii 0.5-1.5 m3/m3hour. You get a purified water outlet with COD 100-300 mg/lSolid state fermentation.Solid state fermentation is carried out in a periodic mode in containers under conditions of mixing and aeration of 20-30 nm3/m3hour. Substrate are conditioned (humidity 70%) and unconditioned (humidity 25-50%) solid waste from palm oil production; solid fraction formed after the liquid-phase fermentation (humidity 65-70%); manure or litter (humidity 80-90%), and excess activated sludge aerated ponds and pond. The fermentation process is carried out at the initial humidity of 60-65%, pH 6.5-7.5, a temperature of 35-45oC during the first two days and periodic stirring 5-6 times a day. In the next day is the self heating to a temperature of 60-65oC, which was incubated for 5-8 days. At this time, there is the destruction of pathogens microflora, helminth eggs, viable weed seeds. After self-cooling get the finished product is a complete compost.
oC, the substrate serve as liquid waste palm oil production with a solids content of 3.5%, which add 6.5% of the dry weight of solid waste (a mixture of residues of analogmike, nutreplodnik, shell, seeds, remnants of pulp - pulp), which previously subjected to acid hydrolysis in the liquid waste palm oil production at pH 3.5, at a temperature of 90oC for one hour. In an environment of growing add 6 g of ammonium sulphate as a source of nitrogen and phosphorus. As a cultural producer using a strain of bacteria Acinetobacter baumanni M-1, which is made in suspension in quantities of 250 ml with a dry matter content of biomass 1, the fermentation Process takes place within 12 hours. The obtained bacterial suspension are separated from the solid fraction on drum sieve and then concentrate on the separator type "Westphalia". Then concentrated biomass is subjected to heat treatment at a temperature of 90oC for one hour and dried in the spray dryer at a temperature of 500-600oC. the result is a finished product - feed protein content crude protein 50%.1.2. The processing sub-standard and condition the radio. Mechanical mixing is carried out periodically 5-6 times a day. The air supply is carried out continuously with the intensity of 20-30 nm3/m3hour. The layer of recyclable solid waste is 1.4 to 1.7 m Unconditioned and conditioned solid waste is a mixture of solids formed after the liquid-phase fermentation with humidity 70%; conditioned pulp with a moisture content of 70%; substandard pulp stored in shoulders with a humidity of 25%; the excess activated sludge aerated ponds and pond. To add solid waste poultry manure with a humidity of 80% at a ratio of 1:1. The content of dry substances in solid state fermentation is 60%. The fermentation process is carried out using associative culture of microorganisms, which are formed by culture-producer with stage liquid-phase fermentation and spontaneous microflora of biocenoses of solid waste and activated sludge. The fermentation process is conducted during the first two days at a temperature of 30-45oC due to self-heating. Subsequently the temperature was raised from 45 to 60oC, which are fermented for 8 days. Then in the next two days undergoing maturation and self-cooling. You get th is B>) is 1.7%, the content of potassium (K2O) is 1.3%. The structure of the compost loose, color brown, dark brown, the smell of wet earth, a pH of 8.3. Pathogenic microflora, helminth eggs, insect larvae and flies, viable weed seeds are not available.1.3, the Treatment liquid flowing after phase separation (see 1.1), is carried out in an apparatus simulating aerated ponds with aeration of 0.5 nm3/m3hour, the temperature of the 35oC, pH 7.0. Stay 5 nights. After cleaning the COD of the liquid flow is 200 mg/L.Thus, the proposed integrated method for processing liquid and solid wastes from palm oil production, characterized by the following features:
- allows you to obtain commercial products in the form of feed protein content of crude protein not less than 50% of the compost as a complete organic fertilizer;
- provides ecological purity of palm oil production, exposing the processing of liquid and solid wastes without any associated emissions or waste to produce clean water, appropriate to the standards for discharge into the water bodies. 1. An eco-friendly way integrated waste proizvodi using selected bacteria cultures Acinetobacter baumanni M-I to obtain fodder protein, compost and purified water.2. The method according to p. 1, characterized in that the recycling is carried out by liquid-phase fermentation of a mixture of liquid and solid waste in the solids content of 10% to produce feed protein content of crude protein up to 50%.3. The method according to p. 1 or 2, characterized in that the solid waste to liquid-phase fermentation is subjected to preliminary hydrolysis at a temperature of 90oC for 1 h, using instead of the acid liquid waste from palm oil production, having a low pH value.4. The method according to p. 1, characterized in that the solid waste is carried out by solid state fermentation using as substrates of conditioned and unconditioned solid waste production of palm oil, solid fractions after liquid-phase fermentation of excess activated sludge aerated ponds and pond, manure or litter obtaining high-grade compost.5. The method according to p. 1, characterized in that at the stage of liquid-phase fermentation is the primary cleaning liquid effluents, and after aerated ponds obtained purified water, appropriate regulations.