The method of obtaining dried yeast
(57) Abstract:The invention relates to food biotechnology and can be used for baking yeast, but rather to get active dried yeast. The yeast is grown in a nutrient medium, where the mineral and organic food uses geothermal water nefrologia class with a defined qualitative and quantitative composition of existing components, in the absence of ions of aluminum and lithium, with a total mineralization of 5.2 to 5.4 g/l, diluted with tap water to a salinity of 4.0 to 4.2 g/l Method allows to reduce the consumption of scarce mineral components and organic food. table 4. The invention relates to food biotechnology and can be used for baking yeast, but rather to get active dried yeast.Known nutrient medium for the cultivation of Baker's yeast that is resistant to drying and rehydration containing ethanol as carbon source, ammonium sulphate, disubstituted phosphate potassium, one-deputizing potassium phosphate, magnesium sulfate, sodium chloride, -indelicato acid and water .The nutrient medium, containing sources of carbon, nitrogen, phosphorus, mineral salts and trace elements, followed by separation of the biomass, drying and dehydration, which is carried out in two stages .The closest is the way to obtain dried yeast, where nutrient medium for growing yeast, raw material for drying and contains uglevodsoderzhashchie materials, such as molasses, as well as a number of substances which are sources of mineral and nitrogen nutrition: ammonium sulphate, diammonium phosphate, ammonia water, potassium chloride, magnesium sulfate, desthiobiotin .Also known is a method of obtaining nutrient medium for the cultivation of Baker's yeast, where the mineral salts used geothermal water in the total mineralization 7.1-7.3 g/l, diluted with tap water to a salinity of 2.0-2.4 g/l of diammonium phosphate and molasses, but the final product is compressed yeast corresponding to GOST 171-81 (RF patent N 2084519)  . The disadvantage of this method is that you cannot use them as raw material for production of dried yeast, corresponding to GOST 28483-90.For reasons that impede the achievement of specified following technical result when ipolish how the process of obtaining yeast time-consuming, much more expensive, because it is associated with additional costs for the purchase of salts; low yield of the final product with lower biotechnology indicators of yeast.The challenge for the solution of the invention is to obtain a highly active dried yeast.The technical result is to increase the yield of yeast, faster and cheaper process, the increase in the number of viable cells during dehydration - rehydration.This object is achieved in that in order to simplify the technological process of production of yeast biomass is the raw material for drying and reducing consumption of scarce components of the original product is grown in a nutrient medium, where the mineral and organic food uses geothermal water with a total mineralization of 5.2 - 5.4 g/l, diluted with tap water to a salinity of 4.0 -4.2 g/l criterion for the selection of natural geothermal water is the absence of radioactivity, phenols, lead, mercury, aluminum, lithium, and the degree of mineralization, the organoleptic properties and the daily yield of water.The advantage of the composition of the used geothermal water in the present invention compared to the s of the N wells 26 and N36 (patent) have different degrees of mineralization: 5.2-5.4 g/l and 7.1-7.3 g/l (patent) and, accordingly, diluted with tap water to a salinity of 4.0 - 4.2 g/l and 2.0-2.4 g/l (patent). Water wells N 26 characterized as sulphate-chloride - hydrocarbonate sodium and has the formula
< / BR>Water well No. 36 (patent) is characterized as a sodium-sulphate-hydrocarbonate sodium and has the formula
< / BR>In addition, geothermal water differ in quantitative and qualitative value of mineral and organic components. The absence of ions of aluminum and lithium from geothermal water wells N 26, unlike geothermal water well No. 36, is a positive factor, as it is known for their toxic effects on living organism. In addition, geothermal water wells N 26, compared with well No. 36, in an advantageous position are quantitatively ions of copper and zinc, with a certain degree of toxicity (g/l environment): copper (0.000001:0.000024) and zinc (0.0000038: 0.000020). However, in natural water wells N 26, compared with the said water has a high content of such important minerals necessary for vital activity of organisms, as (g/l environment): sodium (1.24: 0.74), potassium (0.007:0.005), magnesium (0.0059:0.0031), calcium (0.0085: 0.0056), iron (0.0006:0.00004), manganese (0.0, the geothermal water wells N 26 higher content of organic substances, composed of humic substances (5.36 mg/l environment, the patent - 0.92 mg/l environment), which are stimulants physiological and biochemical processes and activators of membrane rearrangements in the cell. In addition, a significant increase in humic substances in 5.8 times increases its advantage as an ecological factor in the nutrient medium, because they perform a protective function as complexing agents, intensively linking cations of heavy metals, as a priority pollutant in trudnodostizhimye toxic elements or compounds, limiting their bioavailability to organisms. The environmentally clean source as one of the main ingredients of the nutrient medium for cultivation of high quality bakery yeast, raw material for drying is very important for the food industry. Thus, the data state the obvious distinguishing features of qualitative and quantitative composition of mineral and organic substances geothermal water wells N 26 from well No. 36.3. Growing yeast under conditions of aeration and paucity high quality raw materials with homogeneous physiologically active cells, protein fortified and spare carbohydrates, which is of paramount importance in the technology of production of dried yeast, corresponding to GOST 28483-90. Process regulations of the patent N 2084519, using geothermal water well No. 36, similar results cannot be achieved for the reasons listed above.To preserve the enzymatic activity and stability of dried yeast biomass feedstock should be a sufficient amount of protein, spare carbohydrates, mineral elements, such as sodium, potassium, magnesium, phosphorus, calcium. Sodium stimulates growth and reproduction, increases the fermentation activity, emacuulfehact yeast, potassium takes part in the formation of cells of aerobic type; phosphorus - energy processes occurring in the cells in the drying process; magnesium affects the resistance of dried yeast during storage; salts of calcium protects yeast cells from adverse external influences due to compaction of cytoplasm and thickening of the cell membrane. Because yeast is very sensitive to chemicals and their concentrations were carried out a preliminary study on the establishment of Optima yeast cells with a high biomass yield, active enzyme system and a fairly high content of spare carbs and protein.The method of obtaining dried yeast is that at the stage of cultivation compressed yeast, raw material for drying for growing yeast under conditions of aeration in a nutrient medium containing molasses, which is mixed with geothermal water nefrologia class with a defined qualitative and quantitative composition of existing components in the absence of ions of aluminum and lithium, with a total mineralization of 5.2 - 5.4 g/l, diluted with tap water to a salinity of 4.0 - 4.2 g/l when the carbohydrate content of 9.5% and adding diammonium phosphate 2.58 g/L.As optimization parameters were the most important indicators of the process of obtaining yeast: the biomass accumulation of compressed yeast, lifting force, the content of trehalose, protein, micro - and macro-elements, the number of damaged cells relative to control.The proposed method obtained the biomass of yeast with a more sustainable and viable cells, with less permeable membranes, because of the chemical composition and a specific ratio of protein and reserve carbohydrates in the cells. Rather you what s experiments are given in tables 1-4.Example
Cultivation of yeast using the obtained strain Saccharomyces cerevisiae -503 (and. C. the USSR N 1284998) is carried out for 12 hours in-depth method in periodic mode under conditions of aeration (0.25 l/min) in a laboratory setting at 30oC 1, the nutrient medium of the following composition (g/l):
Molasses (in terms of 46% sugar content) - 170-180
The diammonium phosphate - 2.58
Geothermal water mineralization of 4 g/l with a defined qualitative and quantitative composition required for the cultivation of yeast biomass production - raw material for drying - Rest
To add molasses diluted with tap water geothermal water to the content of 9.5% carbohydrates, 2.58 g/l of diammonium phosphate, concentrated sulfuric acid, to achieve in the medium of pH 5, at the rate of 0.4-0.6 ml per 100 g molasses; the contents mixed well. Sterile nutrient medium is poured into 1.5 l in vessels with a capacity of 3 l, then seeded vegetative cultures of the indicated strains in the amount of 12.5% of the sugar content in the medium, 1 ml of which is 63.13106cells. As antifoam use struktol - 0.1 ml/1.5 l medium. The criterion for the end of the process was the reduction of the residual sugar content up to 0.15-0.20%. At the end of the experience from cossy, calculate the biomass yield, molded in the form of bars size 4 x 5 mm, pre-freeze up to t = 20 2oC and dried in the freeze-up to a residual moisture content of 5% at Pleave= 0.4 mm RT.art., drying time is 5 hours.As a control using the method of growing yeast in the medium of the following composition, g/l:
Molasses (in terms of 46% sugar content) - 170-180
The diammonium phosphate - 1.53
Potassium chloride - 1.62
The ammonium hydroxide - 1.13
The ammonium sulfate - 4.6
Desthiobiotin - 0.0000476
Tap water - the Rest
The nutrient composition of the control environment consistent with the GOST 28483-90 (prototype). The process of cultivation of raw materials for drying, obtaining dried yeast as well as in the experience.Table 1 shows the results of a study of biotechnological properties of biomass intended for drying, in a special mode of cultivation. The growth experienced biomass is higher by 21% compared with the control. Together with a high growth rate (generative activity of 0.38 h-1- experience; 0.23 h-1control) tend to have higher lift force, simana, maltana activity, the content of trehalose in the experience of 1.5%, protein - 2.7% higher than in the control. In OPC, cobalt, Nickel, iron, indicating a high physiological value of the original yeast for drying. A significant accumulation of minerals in the experimental biomass, is apparently associated with changes in cell permeability due to the positive influence of biologically active substances of geothermal water in the nutrient medium composition (table. 2). Experienced yeast cells are more uniform in shape and size, with fewer budding 1.7-2%, and more - viable by 8-10%, there is a lack of small cells and cells containing large vacuoles. In controlling the biomass of about 18% of small immature cells that are not needed for the drying process. The results of the study of morpho-physiological, biochemical and technological properties allow the use of experimental nutrient medium in the process of obtaining dried yeast.In table 3 the results of studies that have confirmed the positive impact of experimental nutritional environment for the stabilization of the physiological activity of the yeast during drying and showed a significant benefit of biotechnological properties experienced dried yeast compared with products obtained by techno is Noah particle size and porosity, that contributed to a more rapid recovery of the initial properties in flooding. The security of the experimental cells with trehalose to a greater extent (14.9% - experience, 13% of control) correlated with their survival (80% - experience, 73% of control), a very high ratio of lifting force, maltase and timesnow activity, protein is higher in comparison with control(55:70; 54:58; 28:32; 47.2:40) respectively.Dried yeast, obtained in the result of the experiment, were used for baking bread from a flour of 1st grade. Technological indicators of quality tin bread are presented in table 4. Samples of bread were notable increase in specific volume by 5% with some of the best porosity, dimensional stability. A significant proportion of mineral substances such as calcium, potassium, manganese, magnesium, experienced dried yeast enhances the enzymatic activity of the yeast and the fermentation process in the test. Yeast, rich in minerals with active enzyme system and a high biotechnological indices can be obtained through the use of geothermal water nefrologia class as one of the main ingredients of the technological process of obtaining dried doeskin information sources and identify sources contains information about the analogs of the invention found that the applicant had not discovered similar, characterized by signs, identical with all the essential features of the claimed invention.Thus, the presented data suggest the implementation of the use of the claimed invention, the following cumulative conditions:
The method in its implementation is intended for use in industry, namely to obtain active baking of dried yeast.For the inventive method, it is described in the independent clause sets out the claims, confirmed the possibility of its implementation using the steps described in the application of tools and techniques.Sources of information
1. A. C. the USSR N1521768.2. A. C. the USSR N1713928.3. Semikhatova N. M. Technology of production of dried yeast. M: Food industry, 1976. S.4. RF patent N 2084519 1997 BI N 20.0 The method of obtaining dried yeast, providing the raw material is grown for drying under conditions of aeration in a nutrient medium containing sources of carbon, nitrogen, phosphorus, mineral salts, followed by separation of the biomass, its formation is CA mineral and organic food uses geothermal water nefrologia class with a defined qualitative and quantitative composition of existing components in the absence of ions of aluminum and lithium, with General mineralization 5,2 - 5,4 g/l, diluted with tap water to a salinity of 4.0 to 4.2 g/L.
FIELD: fodder industry.
SUBSTANCE: invention relates to a method for preparing protein-vitamin fodder that involves solid or liquid waste in production and processing the natural raw (grains, milling waste, post-alcoholic distillery grains, beer pellets, fruit pulps or whey). Enzyme lysates are prepared from solid waste and starch waste. Cobalt salt is added to liquid waste or enzyme lysates. Prepared nutrient medium is used in incubation of lactobacillus and propionibacillus microorganisms taken by the following pairs: Lactobacillus acidophilus 1660/02 with Propionibacterium freudenreichii subsp. shermanii 103/12; or Lactobacillus acidophilus 1660/02 with Propionibacterium acnes 1450/28; or Lactobacillus plantarum 578/25 with Propionibacterium freudenreichii subsp. shermanii 103/12; or Lactobacillus plantarum 578/25 with Propionibacterium acnes 1450/28. This method provides preparing fodder enriched with vitamins and proteins and containing live cells of lactobacillus and propionibacillus microorganisms. Method enriches animal intestine microflora after feeding the prepared fodder to animals. Fodder comprises protective substances (organic acids, enzyme systems) and can be stored as crude form for the prolonged time.
EFFECT: improved preparing method, valuable properties of fodder.
13 cl, 1 tbl, 12 ex