A method of obtaining a biocompatible material
(57) Abstract:The invention relates to medicine, namely to ophthalmology, and can be used for the manufacture intracorneal lenses and implants, keratoprosthesis and artificial irises. Spend the bulk radical polymerization of acrylic monomers and/or vinyl series in the presence of water and staple. In the polymerized mixture was added fibronectin or chemically modified fibronectin in the amount of 0.02 to 4.0 mg/ml When the polymerization is carried out using an exposure or by using a real initiation. The invention reduces the number of complications associated with excessive scarring or loss of transparency of the optical media of the eye in the area of the implant. 2 C.p. f-crystals. The invention relates to medicine, namely to ophthalmology, and can be used for the manufacture of intracorneal lenses and implants, keratoprosthesis, artificial irises, etc.A method of obtaining a plastic material made of collagen for ophthalmology (RF patent N 2033165), which is the copolymerization of sorbed on the polysilicon acid collagen monomers of acrylic and/or vinyl series put the it to the tissues of the eye, that is especially important for vnutripolostnyh implants.The objective of the invention is to develop a method of producing a biocompatible material for intracorneal lenses and implants, implants for epikeratophakia, keratoprosthesis and artificial irises, which has specific affinity and adhesion to the connective tissues of the eye, allowing implantation to minimize the process of encapsulation.The technical result that is achievable with the use of the invention is the reduction or absence of complications associated with excessive scarring or loss of transparency of the optical media of the eye in the area of the implant.The technical result is achieved by that conduct bulk radical polymerization of acrylic monomers and/or vinyl series in the presence of water and a binder, and according to the invention in the polymerized mixture was added fibronectin or chemically modified fibronectin in the amount of 0.02 to 4.0 mg/ml, the polymerization is carried out using an exposure or by using a real initiation.Fibronectin is a protein that is a key component of the extracellular matrix. One of the main function is in particularly important when the cornea surgery with implants, when it is necessary to minimize or avoid the formation of a capsule around the implant, reducing the optical properties. Introduction fibronectin in the polymer structure allows adhesion to the extracellular matrix and cells to the polymer by type of natural interaction without forming an additional layer capsule.Chemically modified fibronectin - fibronectin containing one or more reaktionstechnik groups in the molecule. One of the methods of chemical modification of the protein is acylation. The acylation is carried out using an acid chloride of acrylic or methacrylic acid (J. all-Union chemical society them. D. I. Mendeleev, I. XXX, N 4, 1985, 402 - 410).In the case of chemically modified fibronectin is more covalent joining of protein molecules to the polymer matrix. This is especially important when receiving redkostej polymers with high water content.The range of concentration of fibronectin is selected experimentally. For this purpose, we studied the adhesion of cells to the peritoneal number of rats radiated polymers. This was prepared cell suspension in medium 199 and brought a concentration of up to 1,5106cells/ml In the article is the temperature value 37oC for 1 hour, then the solution was decanted and count the cells per unit area of the polymer surface with a microscope. The results were compared with the control, which was a polymer synthesized in identical conditions, but without addition of fibronectin.When the concentration of fibronectin in the polymerized mixture is less than 0.02 mg/ml adhesion of the cells was identical reference samples of the polymer. In the concentration range of 0.02 to 4.0 mg/ml adhesion of cells in the test samples was significantly higher than the control. By increasing the concentration of fibronectin above 4.0 mg/ml did not observe a further increase in the number of adhered cells.Biocompatible material was prepared as follows. To the monomer of acrylic or vinyl series or their mixture was added water, a binder and an aqueous solution of fibronectin or chemically modified fibronectin. The mixture was stirred, was degirolami, saturated with an inert gas, again degirolami, and then polymerizable using radiation, such as gamma irradiation or ultraviolet irradiation. Instead irradiation, the polymerization was performed using real initiators. To this end, in the polymerized mixture dobavljaca illustrated by examples.Example 1. To 78,0 ml of monomer hydroxyethylmethacrylate add 2.0 ml of staple Triethylenetetramine and 20 ml of an aqueous solution of fibronectin with a concentration of 0.1 mg/ml and the concentration of fibronectin in the polymerized mixture is 0.02 mg/ml Solution is stirred, Tegaserod, is saturated with an inert gas and again Tegaserod. The mixture will polimerizuet using gamma radiation dose of 2 mrad. From the obtained polymer by the method of turning were formed intracorneal implants in the form of a ring thickness of 200 microns, with a diameter of 6.5 mm and a hole diameter of 5.5 mmPatient A. 35 years with a diagnosis of right eye myopia of a high degree, the myopic maculodystrophia - operation alloplastic keratophakia with implantation intracorneal implant from FIBROSIS in the form of a ring. The operation carried out on the valve, seamless technique without complications. In the postoperative period, the cornea was transparent, visually defined by a slight swelling of the front flap of the cornea above the lens held on the 2nd day after surgery. By the same date, the cornea has received an estimated ophthalmometry with improvement of visual acuity.Example 2. To 59,5 ml of monomer hydroxyethylmethacrylate add what situation 10 mg/ml The concentration of fibronectin in the polymerized mixture is 4 mg/ml Solution is stirred, Tegaserod and polimerizuet using gamma radiation dose of 2 mrad. From the obtained polymer by the method of turning were formed intracorneal implants in the form of a positive meniscus thickness of 180 microns and a diameter of 5 mmPatient B. 65 years with a diagnosis of right eye postoperative aphakia, complicated hernia vitreous body, "dry" maculodystrophia - operation alloplastic keratophakia with implantation intracorneal implant from FIBROSIS. The operation carried out on the valve, seamless technique without complications. In the postoperative period, the cornea was transparent, visually defined by a slight swelling of the front flap of the cornea above the lens held on the 2nd day after surgery. By the same date, the cornea has received an estimated ophthalmometry with improvement of visual acuity.Example 3. To 65 ml hydroxyethylmethacrylate add 25 ml of an aqueous solution of acidified fibronectin at a concentration of 4 mg/ml and 7.6 ml of water, the solution is thoroughly mixed, Tegaserod and add 0.8 ml of tetramethylethylenediamine and 1.6 ml of 10% aqueous solution of sodium persulfate. Received see the ducks polymer product is recovered from the mold, thoroughly washed and sterilized using gamma radiation.The size of this form is a diameter of 3.5 mm, a thickness of 100 microns. The disk was implanted in the corneal stroma of the rabbit. The implant showed a good biocompatibility. Within 6 months there was no trophic disorders of the cornea. Morphologically did not identify specific infiltration of leukocyte cells and macrophages.Example 4. To 60 ml of water, add 20 g of acrylamide and 40 ml of an aqueous solution of chemically modified fibronectin concentration 1 mg/ml Solution is stirred, Tegaserod and placed into a matrix having the form of a meniscus, with a diameter of 8 mm and a thickness of the center 100 microns. The polymerization mixture was performed in a matrix using gamma irradiation dose of 1.0 mrad. After that, the lens was removed from the matrix, thoroughly washed with water and sterilized.Then decapitalizing the surface of the cornea of the rabbit was placed the lens in the form of a meniscus thickness of 100 microns (edge 20 to 30 microns) with the holes on the periphery, through which it podshivalsya to the stroma. The follow - up period was 2 weeks. Under the bio micro scope on the 3rd day was found barely noticeable Ostrovki cells on the periphery of the lens. A number of them in the follow-up period of up to 2 weeks led produced by scanning electron microscopy lenses. Throughout its surface is detected islet growth of epithelial cells, interconnected "tracks" of cells. Cells are arranged in a monolayer, are flush with each other, uniform in shape. Some cells of smaller size, probably younger. Inflammatory cells are virtually absent.The results obtained indicate that FIBROGEN has a specific affinity to skin cells.Thus, the proposed method of producing a biocompatible material FIBROGEN allows the manufacture of implants for ophthalmic surgery, which has a higher affinity to the tissues of the eye. 1. A method of obtaining a biocompatible material, including bulk radical polymerization of acrylic monomers and/or vinyl series in the presence of water and a binder, characterized in that the polymerized mixture was added fibronectin or chemically modified fibronectin in the amount of 0.02 - 43,0 mg/ml2. The method according to p. 1, characterized in that the polymerization is carried out using an exposure.3. The method according to p. 1, characterized in that the polymerization is carried out with the help of a real initiation.
SUBSTANCE: method involves applying surgical intervention and antitumor therapy. After having removed a part or the whole lung, mediastinum is opened by cutting out horseshoe-shaped or rectangular flap of mediastinal pleura and carrying out lymphodissection. Then, hemostatic sponge is placed in mediastinum. The sponge is impregnated with antitumor chemotherapeutical preparations in intraoperative mode. Pleura is sutured above the sponge. Another hemostatic sponge impregnated with cytostatic preparations in intraoperative mode is attached to visceral pleura of interlobular sulcus.
EFFECT: prolonged chemotherapeutical preparations delivery to malignant neoplasm foci or subclinical metastases.
SUBSTANCE: the suggested multi-purpose heterogeneous collagen matrix for implantation is being flexible-elastic mass obtained out of two collagen sources, moreover, one source is a tissue of vertebrates of one and the same class, and another source - that of an animal of another class, moreover, collagen sources are animal tissues of, for example, mammalian class and avian class, matrix consists of two phases: solid phase - as microspheres out of mammalian tissue collagen, and liquid phase - out of denaturated avian tissue collagen at the ratio of phases being (1-10) : (1-10), at microspheres size being 100-300 mcm, as for final products of biodegradation they are represented by CO2 and H2O. Matrix, additionally, contains components of physiological culture media, and, also, additives that favor the growth and differentiation of cells and tissues, antibacterial and/or antiviral components, and, also, antiaggregation preparations in their efficient quantity, for example, additionally, it contains embryonic cells of nervous tissue. Another aspect of the present innovation deals with the method to obtain the matrix due to preparing mammalian collagen solution (MCS) and denaturated avian collagen solution (ACS), moreover, it is necessary to apply 0.3 M acetic acid, at final concentration for MCS being approximately 0.5-1.5%, and for ACS - approximately 3.0-5.0%, then MCS should be treated with γ -irradiation at the dosage of 1.0 Mrad to be further homogenized to obtain microspheres. Then both MCS and ACS should be washed off with distilled water up to pH of not less than 6.0 and with phosphate buffer solution to mix washed off mammalian collagen and avian collagen at 1:1 ratio at obtaining matrix. The matrix obtained should be additionally supplemented with antibacterial and/or antiviral components, and, also, stimulating agents for tissue regeneration and antiaggregation preparations. The matrix obtained should be sterilized due to γ -irradiation at the dosage of 0.5 Mrad/1 ml. The present innovation enables to obtain new heterogeneous collagen matrix which is considered to be a multi-purpose one applied for transplantology and substitution surgery of different tissues and organs in alive body in case of tissue lesions. Moreover, it is distinguished by controlled terms of biodegradation.
EFFECT: higher efficiency of application.
13 cl, 16 ex, 4 tbl
FIELD: medicine, traumatology.
SUBSTANCE: osseous defect after filling with collapan and usage of metallofixative should be covered with absorbable collagen film that provides orientation of osseous regenerate's organization.
EFFECT: higher efficiency of therapy.
FIELD: peptides, pharmacy.
SUBSTANCE: invention relates to low-molecular derivatives of peptides that are able to act as inhibitors in interaction between laminine and nidogen (interactions laminine/nidogen). Also, invention relates to a method for their preparing, pharmaceutical composition prepared on thereof and their using for preparing pharmaceutical agents, and for identification of inhibitors in interaction laminine/nidogen.
EFFECT: valuable properties of peptides.
5 cl, 12 dwg
FIELD: medicine, experimental medicine.
SUBSTANCE: one should introduce tripeptide Pro-Gly-Pro for laboratory animals as injections at the quantity of 0.09-1.0 mg/kg body weight, and, also, gelatin as fodder additive. The method suggested enables to suppress appetite, decrease the quantity of fodder intake that leads to decreased body weight as a result.
EFFECT: higher efficiency.
2 cl, 5 dwg, 5 ex
FIELD: medicine, dermatology, in particular treatment of trophic ulcer (TU) and long-term open septic wound (LTOSW).
SUBSTANCE: claimed method includes application of wound-healthing composition onto TU and LTOSW in the first step of wound process. Said composition contains (mass %): methyluracil 0.9-1.1; pepsin 3.4-4.4; bentaine hydrochloride 3.6-4.4; sodium chloride 9.0-11.0; and balance up to 100,0: distilled water. In the second and third phases fine cut collagen preparations in form of 3-4 mm thickness layer are applied on purified TU and LTOSW surface. Then cotton carrier freely moistened with wound-healthing composition diluted with water in ratio of 1:2 is layered onto collagen layer. Multihole microirrigator and further sterile cotton carrier are sequentially applied over abovementioned cotton carrier.
EFFECT: effective treatment due to accelerated abruption of necrotic tissues, inhibition of microbial growth, decreased risk of infective, toxic and allergic affects, and improved tissue regeneration.
2 ex, 3 cl
SUBSTANCE: the present innovation deals with the method to accelerate mucosal healing due to the following technique: one should apply a membrane consisted of purified collagenic material obtained out of natural collagen-containing tissue onto the part of affected mucosa to provide the chance for mucosal reconstruction in this part and, also, it deals with mucosa-regenerating preparation and application of purified collagenic material obtained out of collagen-containing natural tissue for preparing mucosa-regenerating preparation. The innovation provides more modified method that accelerates mucosal regeneration, as a whole, and, particularly, after surgical operations associated with the plasty of oral fornix.
EFFECT: higher efficiency.
12 cl, 3 dwg, 5 ex
FIELD: chemical engineering.
SUBSTANCE: method involves pretreating bone tissue of stock-farm animals, comminuting it, precipitating the end product and drying it by lyophilizing. The bone tissue is degreased with alcohol-ether mixture. Demineralization 0.5 and HCl is carried out after grinding during 20 h. Non-resolvable matrix is subjected to proteolysis in HCl of pH=2.0 in pepsin presence at 37°C during 18 h. Then it is centrifuged at 40000g. The end product is precipitated from supernatant with ammonium sulfate to 25% saturation and centrifuged at 6000g. The precipitate is lyophilized against distilled water and chromatographically purified using CM-Sephadex at pH=4.8 and dried with lyophilization.
EFFECT: low cost collagen production; high purity cosmetic preparation production.