The way inulin from tubers of jerusalem artichoke

 

(57) Abstract:

The invention relates to methods of producing inulin from inulinsoderzhathego raw materials. Describes how inulin from tubers of Jerusalem artichoke, including its crystallization and drying, characterized in that from the crushed tubers of Jerusalem artichoke using physico-mechanical separation of water-soluble substances from the water-insoluble fibrous substances tubers get the juice from which by heating to 80-85oC for 1-3 min and filtration to remove protein and colored substances, after which the juice is purified by means of ultrafiltration, diafiltration and nanofiltration, lighten using activated charcoal, concentrated and the resulting solution is crystallized inulin, and separation of water-soluble substances from the water-insoluble fibrous substances tubers carry no later than 5-10 min after grinding of the latter. The proposed solution allows to increase the productivity and profitability of the way of inulin from Jerusalem artichoke.

The invention relates to methods of producing inulin from inulinsoderzhathego raw materials.

Inulin is a carbohydrate from the group of polysaccharides, the meeting shall nerastvorim in cold water, but soluble in hot water, molecular weight /mol.m./ 5000-6000 Yes with an average mol.m. approximately 5400 Yes.

From inulin can be obtained high fructose syrup, crystalline fructose, ethanol and other food products, and also use it as a food additive for regulating metabolic diseases diabetes mellitus, obesity.

According to a known method inulin tubers of Jerusalem artichoke are crushed, add acidified water /pH 5.0/ at ratio of 1 : 2 vol./about. and treated at 70oC for 30 minutes the Mixture was filtered and to the filtrate was added with stirring CaCo3to pH 8-10, and then the precipitate was separated by centrifugation. The supernatant, containing 10-12% inulin, discolor with activated charcoal and evaporated at 40oC under vacuum up to 50% of dry substance. From this solution inulin secrete ethanol /1:2 vol. /about./ and dried at 50-60oC in vacuum conditions /1/.

However, in this method, the same increase process through the use of for extraction of inulin water, acid and chalk. In addition, the spent coolant to heat the reaction mass to 70oC and holding at that temperature for 30 min, and at the UE as a solvent decreases the concentration of inulin in the solution to 10-12%, and processing inulinsoderzhathego solution chalk to pH 8-10 increases okrashivaemoy solution.

A method of obtaining inulin from tubers of Jerusalem artichoke /prototype/ by which inulin is extracted from the crushed tubers extractant - 25% aqueous solution 96oethanol at a ratio of 1:1 and a temperature of 1-4oC for 10 days. After the precipitate supernatant was diluted with distilled water 1:1 and purified by ultrafiltration and ion-exchangers /2/.

In this way the process of extraction of inulin from crushed tubers of Jerusalem artichoke is complex and lengthy. To provide the parameters of the extraction process requires additional costs for the use of the refrigerant, and a continuous process of extraction within 10 days of expensive technology and reduces the specific volume of production per unit of geometric volume of the equipment. The use of an aqueous solution of ethanol as extractant requires additional costs for the organization of fire - proof production. The use of water under extraction entails increased costs for evaporation of the solution when cleaning the product.

The claimed invention solves the problem of simplifying the Lin Jerusalem artichoke.

This is achieved in that in a method of producing inulin from tubers of Jerusalem artichoke, including its crystallization and drying of powdered tubers of Jerusalem artichoke using physico-mechanical separation of water-soluble substances from the water-insoluble fibrous substances tubers get the juice from which by heating to a temperature of 80-85oC for 1-3 min and filtration to remove protein and colored substances, after which the juice is purified by means of ultrafiltration, diafiltration and nanofiltration, lighten using activated charcoal, concentrated and the resulting solution is crystallized inulin, and separation of water-soluble substances from the water-insoluble fibrous substances tubers carry no later than 5-10 min after grinding the last.

Example No. 1. The tubers of Jerusalem artichoke after thorough cleaning and inspection in the amount of 10 kg of pulverized by crushing to a particle size of 0.3 - 1 mm Immediately, within 5 min, obtained from clover begin separating the juice from the solid fibrous substances crushed Jerusalem artichoke by gravity. After extraction of juice /I/ pulp press on the press. The resulting juice /II/ attach to Samotechnaya juice /I/. Protein substances and painted with the>the content of dry matter /DM/ - 21.5 per cent; the optical density /OP/ - 0,41 at length cuvette of 10 mm and a wavelength of 440 nm light. Protein substances and painted compounds are coagulated, part of the coagulant is formed on the surface in the form of a "cap". Division koagulirovat solid particles from the liquid is effected by filtration. The volume of filtrate is 5740 cm3the DM content of 19.5 %, OP 0,11, dobrokacestvennosti 76,15%. The obtained filtrate is light yellow clear of high-molecular substances insulinomas nature, mol.m more than 6500 and colloid-dispersed substances ultrafiltrate on hollow fibers. Concentrate after ultrafiltration of the solution is diluted with water in the ratio 1 : 2 vol./about. and put diafiltration. Permeate after ultrafiltration and diafiltration unite. Amount equal 5580 cm3the DM content of 18.8%, the purity of which is 87.4%. The permeate is then cleaned of inorganic impurities and inuline mol.m less than 4500 Yes nanofiltration, passing it through a semi-permeable membrane with a threshold of retaining 4500 Yes. In ultrafiltrate pass and low-molecular inorganic impurities and inulin accumulates in the concentrate, which contains 26,8% SV, the purity of 94.3%, a pH of 6.7. PTS is a thief in the amount of 0.05 - 0.3% contact at a temperature of 50-60oC for 30-40 minutes, the Coal is separated by centrifugation. This process can lead to dynamic mode. The filtrate becomes colorless by 70-80%. Discolored solution evaporated to ST. 60-65% at a temperature of 55-60oC and vacuum 150-180 mm RT. Art. Of the concentrated solution with a volume of 1500 cm3containing 59.2% of SV, crystallized at 4oC inulin. The output is 740 g c considering returning the mother liquor. After recrystallization from alcohol and water solution and drying receive 710 g of inulin.

The resulting product is analytically determined 96.4% of the main substance /inulin/ angle of rotation []2D0equal to 37.2othe moisture content of 5.8%, a sulfated ash of 0.3%. The permeate is chromatographically homogeneous on the chromatogram detected one brown spot at the start. Impurities of the hydrocarbon in the form of monosaccharides is not detected; pH 6.7; in the lead content of 0.06 mg/kg; cadmium 0.02 mg/kg; Cs-137 less than 4.5 Bq/kg; Sr-90 21,3 Bq/kg; arsenic, mercury and copper are not contained.

Example No. 2. The tubers of Jerusalem artichoke wash thoroughly, inspect, and in the amount of 10 kg of milled at room temperature grated by abrasion to obtain the "clover", which is not later than across from protein and colloidal particles, and the pulp for recycling. In the centrifugation received 7100 cm3juice with a DM content of 21.2%, with an optical density of 0.39 at length cuvette of 10 mm and a wavelength of 440 nm. The juice is heated to 85oC and kept at this temperature for up to 3 minutes Coagulopathies protein substances and colloidal dispersed particles separated from the liquid by centrifugation when the separation factor, equal to not less than 6600. The volume of supernatant is 5700 cm3the DM content of 19.3%, OP 0,09%, the purity of 76.3%. Purification of the obtained supernatant carried out according to the scheme described in example No. 1. From the concentrated solution by volume of 1750 cm3with the content of 56.5% ST inulin is crystallized at a temperature of 8oC for 12 - 18 h, and then inulin is separated from the mother liquor by centrifugation at the separation factor, equal to 6600. Get 710 g wet inulin with regard to returns of liquor. The output of inulin after drying is 450, the product Obtained has the following physico-chemical characteristics: inulin content 97,2%, the rotation angle of 38.5othe moisture content of 5.9%, a sulfated ash of 0.3%. The product chromatographically homogeneous impurities carbohydrates not found.

Sources of information:

1. Abelyan Century A. Receive and note the>/P>2. RF patent N 2001621, A 61 K 35/78, 1993, BI N 39-40.

The way inulin from tubers of Jerusalem artichoke, including its crystallization and drying, characterized in that from the crushed tubers of Jerusalem artichoke using physico-mechanical separation of water-soluble substances from the water-insoluble fibrous substances tubers get the juice from which by heating up to 80 - 85oC for 1 to 3 minutes, and filtering to remove protein and colored substances, after which the juice is purified by means of ultrafiltration, diafiltration and nanofiltration, lighten using activated charcoal, concentrated and the resulting solution is crystallized inulin, and separation of water-soluble substances from the water-insoluble fibrous substances tubers carry no later than 5 - 10 min after grinding of the latter.

 

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