Biologically active additive to food-based nutritional yeast
(57) Abstract:The invention is used in the food industry. Biologically active food Supplement contains selectabase yeast subjected hydroacoustic processing and autolysis, and the autolysate contains not less than 50% of the peptide fraction with a molecular weight in the range of 3 to 24 kDa. Received a dietary Supplement has anti-allergenic and increase efficiency properties, and also extends the range of dietary supplements from nutritional yeast. table 2. The invention relates to food industry, namely, biologically active additives (BAA), obtained from nutritional yeast.Know the use of dried autolysates food Baker's yeast as a General tonic, which supplies amino acids and vitamins SUPPLEMENTS, possessing, however, relatively low nutritional value .The closest in essence to the proposed food additive is BAD, obtained from the autolysate food Baker's yeast , has the property of correction and tonic effect .Technical results of the claimed invention to provide a BUD, region of nutritional yeast.This is achieved through the use of food selenium yeast, which is exposed to sonar processing and subsequent autolysis to achieve in BAD not less than 50% of the peptide fraction with a molecular weight in the range 3 - 24 kDa.The objective of the invention is a product having anti-allergic activity, as well as new biological properties.The tests showed that the product with the desired properties can be obtained only from selenium yeast, which is subjected to acoustic processing, which improves the bioavailability of selenium and its digestibility.Earlier in the prevention of cardiovascular diseases used selectabase yeast, but the application of the autolysate of selenium-containing yeast significantly expands the range of biological effects, at the same time to achieve a positive effect requires significantly less BAD.The positive effect is achieved by the use of new raw materials, and sonar processing and subsequent autolysis allows to obtain a product with the maximum number of biologically active peptides.Below are examples of gender is S="ptx2">Example 1. Dry selectabase yeast dissolved in water to obtain yeast milk and c the concentration of dry matter (DM) 16%. The suspension process in sonar installation and subjected to further autolysis in a thermostat at a temperature of 50oC. the resulting autolysate composition of the peptide fraction is characterized by a molecular weight distribution, are given in table. 1.Example 2. Same as example 1, but dry yeast taken 200 g, the concentration of SP in the obtained fermented milk 18%. The suspension process in sonar installation and subjected to further autolysis in a thermostat at a temperature of 55oC for 16 h In the received autolysate composition of the peptide fraction is characterized by a molecular weight distribution, are given in table. 2.The resulting product is a loose powder, well preserving its properties in the next 12 months.The products of examples 1 and 2 to determine their biological activity was tested on mice and rats.Example 3. Antianaphylactic (Hypo-allergenic) effect of the preparation obtained according to example 1 was evaluated in experiments on laboratory animals (rats) using mods were divided into four groups of 10 animals each.Animals of the first experimental group was senzibilizirani intraperitoneally chicken ovalbumin (OVA) and, starting from the first day of sensitization, during 28 days was introduced in addition to fivarname diet intragastrically using a probe 120 mg yeast autolysate prototype and 60 mg of the product of example 1 (6,0 ág selenium per rat per day) in 2.0 ml of physiological solution.Animals of the second experimental group was senzibilizirani OVA similar animals first experimental group. In addition to fivarname ration they received intragastrically 180 mg of yeast autolysate prototype in 2.0 ml of physiological solution.Animals of the third experimental group was senzibilizirani OVA as well as animals first and second groups. They only had vivary diet.Animals-fourth (control) group did not senzibilizirani and kept on viburnum diet, entering daily intragastrically in 2.0 ml of saline. Sensitization was carried out according to the following scheme: in the first, third and fifth day, rats were injected intraperitoneally at 100 mg OVA with Al2(OH)3and on the twenty-first day booster injection 10.0 mg OVA with Al2(OH)3.On the twenty-ninth day sensibilizing. The severity of the symptoms of anaphylactic shock was evaluated by intestinal barrier, which is determined by the absorption of the indicator intestinal permeability of polyethylene glycol with a molecular weight of 4000 daltons (PEG-4000). The absorption of PEG-4000 was determined in turn by its excretion in the urine, which was analyzed using gel chromatography on a column of Toyopearl HW-50 . At the same time in urine colorimetrically was determined by the creatinine concentration . The absorption of PEG-4000 was expressed in % per 1 mmol secreted creatinine. The results of this determination are the following: the reception of Vitacel" reduced the severity of symptoms of anaphylaxis compared to sensitized animals of the third group by 25%. The reception of Vitacel-Se showed a positive effect on the state of the protective barrier of the small intestine sensitized animals, significantly reducing their severity of anaphylactic shock by 40%. The effect of this drug was so strong that defined the indicator does not differ from the corresponding index in the intact animals (control group). About the efficiency of utilization of animals selenium is part of Vitacel-Se", demonstrated by the increase of its content in blood serum and Zirovanii animals and has antianaphylactic (Hypo-allergenic) effect.Example 4. The influence of adaptation to physical loads of preparation example 2 was studied in experiments on mice. Mouse strain CBA were divided into two groups of 25 animals each. Animals of the experimental group during the 6 days were additionally by intragastric administration (probe) 3 mg "Vitacel-Se" and 20 mg of Vitacel in 0.1 ml of physiological solution against the standard diet. Animals of the control group were on standard vivarium diet. On the seventh day evaluated the performance of the animals in both groups by testing the length of their voyage (test "voyage to drowning") at a water temperature of 16oC. the Results of a study in which the time to drowning animals of the first group on average one and a half times more than the second, indicate a substantial, statistically significant increase in exercise tolerance in mice that received as a Supplement to fivarname diet "Vitacel-Se".References
1. Patent of Russia No. 2003262, CL A 23 J 1/18; A 61 K 35/66; A 61 K 35/78
2. Patent of Russia No. 20875531, 1997 - prototype.3. Materials of scientific conference "New information technologies in medicine and ecology". Ukraine, Crimea, Yalta-Gurzuf, may 1997,5. Biological methods of research in clinical practice (Ed. by A. Pokrovsky, A.). Biologically active additive to food-based nutritional yeast containing nutritional yeast autolysate, characterized in that the quality of nutritional yeast use selectabase yeast subjected to acoustic processing, the autolysate which contains at least 50% of the peptide fraction with a molecular weight in the range of 3 to 24 kDa.
FIELD: microbiological and food-processing industry, in particular, production from yeast biomass of biologically active food product enriched in components valuable for human feeding, such as phytoteas, beverages, various food products and dishes.
SUBSTANCE: method involves washing and removing mustard from food yeast; providing autolysis, boiling out and drying procedures. At autolysis stage, yeast suspension is diluted with water used in the ratio of 1:1 at temperature of 500C and pH value of 5; holding while mixing for 60-90 min. After autolysis operation, preliminarily diluted enzyme preparation of amylorisin is introduced into diluted mass in an amount of 0.2% by weight of basic amount of yeast and held at temperature of 500C for 5-6 hours, with mixing being provided during holding period. Mustard is removed from food yeast by means of sodium bicarbonate. Method allows clear and transparent product with increased content of substances useful for human organism to be obtained.
EFFECT: simplified method, wider range of use and improved quality of ready product.