The strain of filamentous fungus - producer substances that stimulate the growth and development of lupine

 

(57) Abstract:

The invention relates to the production of microbiological agents that stimulate the growth of plants, in particular lupine. The strain of filamentous fungus Mortierella stylospora Dixon-Stew deposited by the all-Russian collection of microorganisms IBPM RAS N VKM F-3604., He synthesizes biologically active substances both in the mycelium and releasing them into the culture fluid. Processing of Lupin seeds of 0.001% solution of the extract of the mycelium or the culture fluid stimulates plant growth lupine. Joint processing of Lupin seeds extract of the fungus and root nodule bacteria helps to increase the yield of lupine grains up to 8 t/ha table 3.

For General information.

According to the literature data it is known that microorganisms associated in nature with plants, there are not only pathogens, but also inducers of immunity, Symbiotropin, stimulants and inhibitors of growth, representatives of other physiological groups.

Symbiotropin lupine are nodule bacteria. However, we have found on the roots of the lupine symbiotrophic mushroom, morphologically corresponding mean Mortierella stylospora Dixon-Stewart (Mucorales).

The fungus isolated in pure culture and done is the Reda and save physiological properties during long-term storage of cultures and subcultures.

2. Morphological and cultural characteristics of the strain.

Colonies of strains grow well on we tested nutrient media: potato-glucose agar and agar of čapek.

Mycelium white, cobwebby, 7 day growth mode 23-24oC, reaches a diameter of 6-8 cm and a height of 2-2,5 cm Colonies grow in concentric zones. In mycelium formed numerous dark spherical asiaspace 12-20 µm in diameter. Other types of sporulation is not formed.

3. Physiological properties of strain.

3.1. Presowing cultivation of seeds of lupine in the homogenate of the colony of the fungus grown on potato-glucose agar or agar of čapek, affects the plant, reducing the height of the stalks in the field experiment by 40-60%.

3.2. Extracts from the colonies of the fungus at pre-sowing treatment of seeds have to plant different effects depending on the concentration of the solution (table 1).

Experiments by the method of growing plants in rolls (without contact with the soil) showed that the biologically active substance of the fungus at low concentrations have a stimulating effect.

3.3. The effectiveness of biostimulation growth of lupine in the greenhouse.

Seeds lupicia observations.

The test results are given in table 2.

Thus the extracts of the fungus have a stimulating effect on the growth and propagation phases of development of lupine. The most active is expressed by using an aqueous extract at a dilution of 1:1000.

3.4. The test extracts of the fungus in small-plot field experience.

The experience made in the fields all-Russian research Institute of lupine (,Bryansk) in 1995.

On the day of sowing lupine seeds treated nodule bacteria - strain a and water mushroom extract (ratio 1:1000). Seed treatment with the extract of the fungus obtained statistically significant at 5.3 t/ha increase in grain yield to the control without seed treatment. Established a highly stimulating effectiveness of the extract of the fungus compared to seed treatment nodule bacteria (table.3). The combined use of nodule bacteria and mushroom extract had a more stimulating effect on plants. Plants lupine on this variant differed thick stalk, intense green colour of the leaves. On the variant with the joint application of nodule bacteria and mushroom extract set the highest increase in grain yield of 8.0 kg/ha to control and 5.9 t/ha of green mass yield of lupine. Increase dry matter of green mass made up 37.4 kg/ha to control and 32.7 kg/ha to the reference variant (PL. 3).

The strain of filamentous fungus Mortierella stylospora Dixon-Stew VKM F-3604 D producing substances that stimulate the growth and development of plants lupine.

 

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FIELD: biotechnology.

SUBSTANCE: the present innovation deals with immunization in plants, particularly, compositions and methods for inducing plant resistance to phytopathogenic organisms, such as phytopathogenic fungi. An agent indicated for inducing plant resistance to phytopathogenic microorganisms is being an extract out of biomass of non-phytopathogenic microorganisms. The method to obtain the above-mentioned agent includes the following stages: a) resuspending against 50-200 g (dry weight) biomass of non-phytopathogenic microorganisms in 1 l either inorganic or organic solvent, b) mixing at room temperature for 1-12 h, c) incubating, d) resuspending, e) cooling up to room temperature at maturing and f) filtrating, not obligatory. The innovation enables to induce plant resistance to phytopathogenic microorganisms.

EFFECT: higher efficiency of immunization.

12 cl, 6 ex, 5 tbl

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