The way to identify the effects of radiation on biological object

 

(57) Abstract:

The invention relates to medicine, in particular to methods of bioindication mainly small doses of radiation, and may find application in medicine and radiology. Determine a representative number of subpopulations of erythrocytes, reproduce morphogenically the reconstruction of all subpopulations and identify radiation exposure according to the characteristics of changes in their structure in the plane of observation and the structure part of the biological object. As a representative of a number of subpopulations of erythrocytes using disco and ferocity. The method provides increased reliability and accuracy of the information. 7 C.p. f-crystals, 4 Il.

The invention relates to methods of bioindication mainly small doses (less than 0.05 gr.) radiation and may find application in medicine and radiobiology.

Currently, medical ecology has developed a new area of research - the effect of small doses of ionizing radiation on biological objects. Examples of such radiation in life are: tower, microwave ovens, computer monitors, cordless phones. Due to new research concept appeared elec is medicinska studies have shown, what is the key element of the violation Biosystems intracellular violations and intercellular transfer of information. As we know from the biophysical and biochemical research - based information transfer is the conformational transition in the grounds of the NPD (the interphase chromatin) and biomembranes. The study of these processes is a necessary step to determine the effects of non-ionizing radiation.

The closest in technical essence is a Method for diagnosing exposure to radiation", consisting in the determination of damage to the genome, based on the definition of disconnection in DNA(1). However, the rapid and efficient DNA damage repair within 1 hour after exposure to radiation makes its practical application.

The essence of the method lies in the fact that as part of a biological object using supramolecular structures within intact cells, such as erythrocytes. As parameters to identify the effects of radiation can choose the form and volume of red blood cells before and after exposure to radiation, i.e., the number of disco and spherocytes, as well as their ratio. Moreover, a key bioindicator in erythrocyte selected biological Membranula the characteristics of images of 2-dimensional patterns in the plane of observation and the 3-dimensional structure of a biological object by measuring in the plane of observation and the following dependencies:

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where R is the distance element of the erythrocyte membrane (e.g., spicules) to the center of the erythrocyte;

- the angle in polar coordinates the orientation of the radius vector relative to the normal plane;

the orientation angle of the membrane element in the plane of incidence and convert these measurements into quantitative characteristics of the structure.

When changing subpopulations of erythrocytes after exposure, ask a representative number of subpopulations, for example, disco and ferocity, and changes to determine the impact. Also reproduce 3-dimensional MDM reconstruction of all subpopulations of erythrocytes before and after exposure, it is also possible partial (selective), for example, a representation class.

The technical result is expressed in consideration of the range of detection of the impact, improving the reliability and accuracy of the information, etc.

The invention is illustrated graphics, where: Fig. 1 shows a fixed IPS subpopulations of erythrocytes and their location in the control and irradiated the blood smears 1 and 2 mice, Fig. 2 is a three - dimensional reconstruction of the membrane discocyte, Fig. 3 and 4 three-dimensional reconstruction of the membrane with partial variance ">

As the primary biological indicator of exposure to low doses of radiation on biological object are encouraged to use the erythrocyte, and more specifically its biological membrane. This is due to the fact that this cell:

1) is present in most structures of the biological object, i.e. it is ubiquitous, for example, during the day in every part of the blood it is present three times.

2) due to their structure, namely, the presence of hemoglobin, which is the carrier of oxygen.

The aim of the experiment is to evaluate the changes:

1) shape of the erythrocyte;

2) its rheological properties.

In addition, try to assess the oxygen saturation of hemoglobin before and after exposure to a biological object, in particular blood, low dose radiation (of the order of 0.05 j/kg), for example, emitted by the computer.

Thus, to create a test system for evaluation of the impact of physical therapy on the human body.

As the source of biological material for research was used the blood of 2 of the experimental mice. For morfogeneticheskogo analysis of blood of each mouse was placed in two capillary vessel, and for rheological analysis in two UNDP radiation, was the area of least impact, where they set up a control sample (source 2 of capillary vessel and 2 tubes of blood, one from each mouse).

When the computer monitor in front of the screen at the level of the location of the operator by using the "frame" was defined area of influence from the monitor, where he subsequently established similar to the control samples, the remaining party 2 capillary vessel and 2 tubes of blood (one from each mouse).

The impact from the monitor blood was carried out for 10 minutes. From the blood in 4 capillary vessels exposed and non-exposed, made the strokes, and blood in 4 test tubes were tested for rheological properties.

After making blood smears before and after exposure was carried out their study on opto-electronic morphodencitometric system (IPS).

Control swabs and smears, exposed, placed in the IPS, where by a special technique was conducted studies of erythrocytes in them, namely, composition (subpopulations).

In Fig. 1 in the top row (left to right) presents fixed IPS subpopulations of erythrocytes and their location the scholar.

Identified representative subpopulations, namely, disco and ferocity.

From the picture, shown in Fig. 1, it is revealed that after exposure to radiation significantly decreased the number of discocytes and dramatically increased the number of spherocytes. In addition, after exposure increased the number machinewith cells and appeared leptonica.

Thus, we can assume that part of discocytes transformed into ferocity. Based on the above analysis was made of the statistical processing of the received data using the conversion Fisher and built eritrotsitami for each case.

After that, each subpopulation (can only representative of erythrocytes) martinstoller.com method were processed and constructed three-dimensional reconstruction of the membranes of each of the erythrocyte. This allowed to identify with greater certainty the nature of the effects of radiation, i.e., to define even a slight deviation of the profile of the membrane.

Further, similar studies were carried out on computers with different background radiation and are obtained from the corresponding results.

Method three-dimensional MDM-reconstitue structure mortalit. For its implementation produce a dimension in the plane of observation and using the formula:

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< / BR>
where R is the distance element of the erythrocyte membrane (e.g., spicules) to the center of the erythrocyte;

- the angle in polar coordinates the orientation of the radius vector relative to the normal plane;

the orientation angle of the membrane element in the plane of incidence.

This method was tested in an area with low background radiation after the accident an HOUR, and was confirmed in people living in areas of minor contamination. There is an imbalance between disco and spherocyte blood (the change of a biological membrane of an erythrocyte), which led to a significant increase in morbidity (II).

Suggested method allows to detect changes in biological membranes of red blood cells in the early stages of the disease, and is a test system for evaluation of the impact of physical therapy on the human body.

Sources

1. "Radiobiology", Mikhailov C. F., Potemkin, L. A., T. 25, vol. 6., pages 54-55, 1985 - prototype.

2. "Changes in the pool of peripheral lymphocytes of children living on the contaminated territories", Boykin C. M., "the dissertation of the candidate of med.) - Rev. make a comparison of the structures part of the biological object and their parameters before and after exposure to radiation, and determination of their changes, characterized in that as part of a biological object using the erythrocyte determine a representative number of subpopulations, and then reproduce morphogenically reconstruction of a representative number of subpopulations and identify radiation exposure according to the characteristics of changes in their structure morphodencitometric reconstruction characteristics obtained prior to the impact.

2. The method according to p. 1, characterized in that as a representative of a number of subpopulations using disco and ferocity.

3. The method according to PP.1 and 2, characterized in that use quantitative indicators disco and spherocytes.

4. The method according to PP.1 to 3, characterized in that use a ratio of disco and spherocytes.

5. The method according to PP.1 to 4, characterized in that as part of a biological object using the membrane of the erythrocyte, and identify the impact on changing her profile.

6. The method according to p. 5, characterized in that reproduce 3-dimensional morphogenetics reconstruction of all subpopulations of erythrocytes before and after exposure.

7. The method according to PP.5 and 6, characterized in that reproduce 3-dimensional morpho is audica fact, what 3-dimensional morphogenically reconstruction reproduce by identifying static mapping characteristics of the two-dimensional image of the structure on the plane of observation and the 3-dimensional structure of a biological object by measuring in the plane of observation and the following dependencies:

< / BR>
< / BR>
where R is the distance element of the erythrocyte membrane (e.g., spicules) to the center of the erythrocyte;

- the angle in polar coordinates the orientation of the radius vector relative to the normal plane;

the orientation angle of the membrane element in the plane of incidence and converting these measurements into quantitative characteristics of the structure.

 

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