The way to determine the level of iodine deficiency in the population

 

(57) Abstract:

The invention relates to the field of biological and medical research. The method provides a reduction in research time and reduce the complexity. Produce a sample of urine, making the sample a solution of salts of carbonic acid, calcined mixture, washing the residue with water, centrifuged, give centrifugal chemical reagent, which is used alternately introduced into centrifugal aqueous solution of potassium thiocyanate with a concentration of 0.6 g/DM3, an aqueous solution of sodium nitrate with a concentration of 20 g/DM3and an aqueous solution salesonline alum with a concentration of 100 g/DM3when a volume ratio of these solutions 1:2,5:4, respectively, heat the solution on a water bath to 30C, measure the optical density of the solution at a wavelength of 430 nm and a calibration curve to determine the concentration of iodine, which is judged on the level of iodine deficiency among the population. table 1.

The invention relates to the field of biological and medical research, in particular to methods for assessment of the severity of iodine-deficient state population method of determining the iodine content in urine, and can be used in carrying out the population for the development of preventive measures.

In recent years the Russian Federation has experienced an increase in the incidence of diseases associated with insufficient intake of this trace element, as iodine. Deficiency of vital trace element causes the formation of a spectrum of pathological conditions in humans, namely: hyperthyroidism (endemic goiter), retarded mental and physical development (endemic cretinism), impaired vision, deviations from musculoskeletal, impaired development of the reproductive function, deafness, etc.

To assess the severity of iodine deficiency among the population of the world health organization (who) proposes to use such a criterion, as the average level of iodine excretion in the urine, and when this figure 5-10 g% diagnosed with mild iodine deficiency; 2-5 g% - average degree and less than 2 mg% - severe iodine deficiency. Moreover, who found that in a population with mild iodine deficiency may increase thyroid cancer in 10-20% of the population with average - to 20-50% and severe (more than 50%. Moreover, this approach is due to the fact that iodine, which is excreted in the urine enters the human body produced can be used as an estimate iodine-deficient state of the population.

There is a method of determining the level of iodine deficiency among the population by examination of the urine, according to which produce fence urine sample, then the sample volume of 1 ml add 1 ml of 10% aqueous solution of zinc sulfate and 2 ml of a 2N solution of sodium carbonate, the resulting mixture was dried in a thermostat at 97oC, then calcined at 600oC for 2.5 h, cooled and rinse with water (6 ml), then centrifuged 10 min and 2 ml of centrifugate add 2 ml of a mixture of "COP", which is prepared by mixing 500 ml of water 120.5 ml of concentrated sulfuric acid; the resulting mixture of centrifugate and "CS" is placed in an ice bath (+4oC) for 10 minutes, then add 2 ml of chilled solution of cerium sulfate ammonium 0,0005 M, mix and produce heat on a water bath (40oC) for 20 min; then again placed in an ice bath for 10 min, add 0.5 ml of 1% aqueous solution of brucine and mix again heated for 15 minutes at 100oC and then measure the total optical density of the resulting solution on a spectrophotometer at a wavelength of 430 nm, carry out the calculation of iodine concentration on the calibration curve, and this concentration is judged on the level of iodine devicecapability endocrinology", 1963, No. 6, S. 64-68). The disadvantages of this known method are the complexity of the duration and complexity.

The aim of the present invention is to simplify the method, reducing the time of its performance and reducing the complexity.

This objective is achieved in that in the known method of determining the level of iodine deficiency among the population by examination of the urine, including a sample of urine, the introduction of the sample solution of salts of carbonic acid, calcining the mixture, washing the residue with water, centrifuging, the introduction of centrifugal chemical reagent and heating the solution on a water bath, followed by measurement of optical density of the solution at a wavelength of 430 nm and the calculation of the calibration curve the concentration of iodine, which is judged on the level of iodine deficiency among the population, what is new is the fact that as a solution of salt of carbonic acid used 3%aqueous solution of potassium carbonate, as a chemical reagent used alternately introduced into centrifugal aqueous solution of potassium thiocyanate with a concentration of 0.6 g/DM3, an aqueous solution of sodium nitrate with a concentration of 20 g/DM3and an aqueous solution of iron ammonium alum with a concentration of 100 g/DM3oC.

Neither of the patent or scientific literature we do not know how to determine the level of iodine-deficient state in population, through the above set of operations that allows to make a conclusion about the "newness" of the proposed solution.

However, as unexpectedly, the use of urine study as a solution of salts of carbonic acid 3% aqueous solution of K2CO3as a chemical reagent solutions of specified concentrations of potassium thiocyanate, sodium nitrite and iron ammonium alum which are entered one by one in this order and in the specified ratio, provides a simplified way, reducing its complexity and duration due to the fact that excluded a number of operations and simplified modes of definitions that have been determined experimentally. This allows to make a conclusion about the relevance of the proposed solutions to the criterion of "inventive step".

The proposed method is as follows:

- produce a sample of urine;

- add a 3% solution of potash;

- the mixture is evaporated to dryness;

- the remainder ol the n within 30 minutes;

- give centrifugal alternately with stirring, a solution of potassium thiocyanate with a concentration of 0.6 g/DM3solution of sodium nitrate with a concentration of 20 g/DM3and a solution of iron ammonium alum with a concentration of 100 g/DM3when a volume ratio of these solutions as of 1:2,5:4, respectively;

the mixture is placed for 15 min at 30oC in a water bath;

then the resulting solution photometrate on the spectrophotometer at a wavelength of 430 nm;

- determine the concentration of iodine in the urine sample from the calibration curve, which pre-build;

- at a set concentration of iodine in the urine sample is judged on the level of iodine deficiency in the human body, defining the scale who mild, moderate and severe iodine deficiency.

The proposed method was tested in the laboratory of biochemistry and toxicometric Perm NICKY children's environmental pathology. This was formed by a group of children in the amount of 96 people aged 5 to 7 years, living in, Perm.

Each child took a urine sample (morning dose). Next, each sample was subjected to study in the following way. When the research took reagents mark "H. h".

In the crucible was placed 10 ml of the analyzed urine and dobivali in a muffle furnace at 480oC for 30 minutes, Cooled in a desiccator to room temperature. The residue was treated with water by 4-fold add 2.5 ml of water. The solution was centrifuged at a speed of 2000 rpm for 30 minutes and Then 4 ml of centrifugate alternately with stirring was added 0.4 ml of a solution of potassium thiocyanate with a concentration of 0.6 g/DM3, 1 ml of an aqueous solution of sodium nitrite with a concentration of 20 g/DM3and 1.6 ml of an aqueous solution of iron ammonium alum with a concentration of 100 g/DM3. The resulting mixture was placed for 15 min at 30oC in a water bath. And then using a spectrophotometer determines the optical density of the resulting solution (used cuvette 10 ml) at a wavelength of 430 nm. Previously on the same spectrophotometer constructed a calibration curve, which was determined by the concentration of iodine in the urine sample.

Then at a set concentration of iodine in the urine sample was defined mild, moderate or severe iodine deficiency each child of the target group of children and, on this basis, judged the severity of iodine-deficient state in the territory of their residence.

The obtained data are given in the table.

The results of the study n the AI who.

On the basis of the obtained results was given epidemiological assessment of prevalence and severity of iodine deficiency in the territory, Perm. According to who criteria the territory assigned to the zone of moderate endemic. Actions have been developed for the treatment of the surveyed children (by assigning thyroid hormone and iodine preparations and activities for the prevention of iodine-deficiency conditions in children of this area (the inclusion of iodine additives in food - bread, salt, confectionery and others).

The proposed method is simple, accessible and does not require a large investment of time and funds for its implementation.

Through the use of the proposed method in medicine, the opportunity to determine the level of iodine-deficient state of the broad masses of the population, which allows you to set the priority and amount of preventive measures to overcome iodine deficiency in some areas, and consequently, to improve the health status of the population.

The way to determine the level of iodine deficiency among the population by examination of the urine, including a sample of urine, the introduction of the sample solution of salts of carbonic acid, calcining the mixture, washing the residue with water, centrif the measurements of the total optical density of the solution at a wavelength of 430 nm and the calculation of the calibration curve the concentration of iodine, which judge about the level of iodine deficiency among the population, characterized in that as a solution of salts of carbonic acid using 3% aqueous solution of potassium carbonate, as a chemical reagent used alternately introduced into centrifugal aqueous solution of potassium thiocyanate with a concentration of 0.6 g/DM3, an aqueous solution of sodium nitrate with a concentration of 20 g/DM3and an aqueous solution salesonline alum with a concentration of 100 g/DM3when a volume ratio of these solutions 1 : 2,5 : 4, respectively, while heating the solution on a water bath to carry 30oC.

 

Same patents:
The invention relates to medicine and relates to the selection of homeopathic medicines
The invention relates to medicine, namely to cardiopulmonary, and can be used to investigate the state of the cardiovascular system and respiratory organs

The invention relates to the field definition quality dry food for dogs and cats

The invention relates to medicine and can be used to diagnose as the outer surface of the skin and internal organs, for example for the diagnosis of cervical cancer

The invention relates to agriculture and can be used to determine the quality of canned feed for dogs and cats

The invention relates to medicine, to methods of evaluating the effectiveness of drugs and can be used in experimental studies as a means of screening assessment of the efficacy of potential drugs for nitrosomonas therapy of tumors

The invention relates to medicine, in particular to pharmacognosy and is intended for the qualitative and quantitative determination of components in multicomponent herbal powder charges
The invention relates to experimental Hematology and applies methods for studying regenerative capacity of bone marrow in vivo

The invention relates to medicine, namely to Phthisiopulmonology, and can be used in the diagnosis of limited forms of pulmonary tuberculosis
The invention relates to medicine and relates to the selection of homeopathic medicines

The invention relates to medical technology and practice, in particular to methods of non-invasive diagnostics of functional state of biological membranes, analysis and evaluation of activity elements arc somatic reflex, reaction, reflex response to standard sensory stimulus and the nature of the metabolic processes in the body at the cellular level in terms of application of constant magnetic fields (MFS)
The invention relates to medicine, particularly cardiology, and can be used to predict coronary death within one year after myocardial infarction

The invention relates to medicine and relates to a method of diagnostics of the functional state of the stomach and duodenogastric reflux in patients with allergic diseases (atopic dermatitis, bronchial asthma, etc

FIELD: medicine, psychiatry.

SUBSTANCE: one should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.

EFFECT: more objective prediction of disease development.

3 ex

Up!