The method of identifying components of plant collecting microscopic method
(57) Abstract:The invention relates to medicine, in particular to pharmacognosy and is intended for the qualitative and quantitative determination of components in multicomponent herbal powder charges. The method provides improved quality control and simplifying it in the standardization of fees type "tan". Microscopic determination of components in the collection, make use of a grid, sequentially performing the operations of determining the number of particles of each component of the collection, and then calculate the index participation component Assembly quantitative component to the total Assembly. 3 table. The invention relates to pharmacognosy and is intended for the qualitative and quantitative determination of components in multicomponent herbal powder charges.The most common methods of analysis of multicomponent mixtures of vegetable origin are physico-chemical methods of analysis of biologically active substances.Existing methods for the analysis of herbal infusions require the development and use of multiple methods of analysis, taking into account all biologically activeholidays the analysis process and increases its cost.Many of the methods used are expensive and require highly skilled personnel.Quantitative assessment of herbal remedies on the amount of substances present in two or more components of herbal, does not give an accurate representation of the percentage of each component in the collection. Eventually faces the problem of finding a method of quality control and standardization compositions medicinal plants.Microscopic analysis method is less time consuming and more accurate in comparison with chemical and physico-chemical methods of analysis, where the substances are not representatives of the major groups of active substances may behave similarly to the latter, thereby distorting the results or requiring a more thorough cleaning of the main group of biologically active substances. However, the use of microscopy this purpose in the practice of pharmaceutical analysis is limited due to insufficient development of this method and because of the tradition of the application of well-developed methods of analysis. Still this method of analysis was used primarily to confirm the authenticity of medicinal plants, to assess its quality.The aim of the invention is to improve the quality control and simplifying it in the standardization of fees type "tan".This objective is achieved in that the microscopic determination of the components of the Assembly is performed using the grid sequentially the operation of determining the number of particles of each component of the collection and the index participation component Assembly quantitative component to their population in the collection.In the analysis allowed the presence of particles, which is characteristic only for the components of the Assembly. Basic anatomical and diagnostic characteristics of the powders of the components of the Assembly "Nard-4" is presented in the table. 1.To confirm the results are qualitative reactions according to standard techniques. Characteristic microscopic qualitative reactions of the components of the Assembly "Nard-4" are shown in table. 2.Method for quantitative determination of the components of the Assembly "Nard-4" in three different versions. Use 3 modification of the charge ratio of the components: the roots of elecampane, black elderberry flowers, roots of Sophora yellowish, ginger rhizome Le is as in the analysis of the qualitative determination of components of the Assembly. Next, count the number of particles of each component under the microscope (magnification 7(15) 1,5 10(20)) in the taken sample (about 1 mg) on a glass slide separated largest glass cover (16 x 16 mm) for 16 squares with a side of 4 mm, a nail file or a pencil on the glass.The calculation of the index participation elderberry is calculated by the formula
< / BR>where
a is the number of particles of an elder;
b - the number of particles of Valerian;
c is the number of particles Sophora;
d is the number of particles ginger.Similarly, calculate the index of participation for other components.The index participation components must be within the limits specified in table. 3.The obtained results were statistically processed, the method error does not exceed 5%.The developed method allows to determine with high precision the number of components in powdered 4-component Assembly "Nard-4". This herbal Tibetan medicine has several variations of the ratio of components, each of which has a different purpose and different pharmacological activity, so determining the number of components of the collection is of great importance to obtain the drug, with varying farmitalia Assembly, including qualitative determination of the components of the collection microscopic method, characterized in that the further conduct consistently operations determine the number of particles of each component of the collection using the grid and then calculate the index of participation of each component in the collection for the quantitative component to the total Assembly.
FIELD: microbiology, pharmaceutical agents.
SUBSTANCE: invention relates to method for determination of genome response of specific cells to vegetable extract action. Method for drug screening includes 1) treatment of specific cells with vegetable extract; 2) protein or RNA isolation from said treated cells; 3) identification of isolated protein or RNA; 4) determination of compound(s) in said vegetable extract; 5) treatment of said cells with determined compound(s); 6) protein or RNA isolation from said treated cells; and 7) determination of compounds providing expression or suppression of said protein or RNA which have another concentration than in untreated said specific cells.
EFFECT: identification of individual compound(s) for screening of new pharmaceutical agents or new pharmaceutical application of existing drugs.