The method of modeling constipation

 

(57) Abstract:

The invention relates to medicine, specifically to experimental physiology, pharmacology and pathophysiology related to the possibility of modeling violations of motor-evacuation function of the intestines - constipation. This object is achieved by the introduction of rats intragastrically 10% suspension of activated charcoal in a total dose of 2 g/kg Method can be used in research when studying the mechanism of constipation and means possessing laxative effect. table 1.

The invention relates to medicine, specifically to experimental physiology, pharmacology and pathophysiology, comes to play model constipation and can be used in scientific research in the study of means, having a laxative effect.

There is clinical evidence that the development of disorders of motor-evacuation function of the intestines - constipation can be caused by organic diseases and functional disorders resulting coprosmas, usually in the rectum and sigmoid colon. In the pathogenesis of constipation essential dyskinesia bowel (spastic or mixed), inflammatory changes, dibetou us literature is not found.

The problem solved by the invention is the creation of a model that displays the status of constipation in the experiment.

This goal is achieved by intragastric administration of 10% suspension of activated charcoal in a total dose of 2 g/kg

New in the invention is that intragastrically administered 10% suspension of activated charcoal in a total dose of 2 g/kg

The invention meets the criterion of "inventive step" because it is not obvious to a person skilled in the art. The invention meets the criterion of "industrial applicability" because it can be used for the experiment.

The proposed method of modeling constipation realized in experiments on outbred rats male weighing 190 to 210 g (n = 34), which twice was administered intragastrically 10% suspension of activated charcoal with an interval of 40 min to 1.5 h in a total dose of 2 g/kg of a Suspension of activated carbon prepared 1% solution of starch mucus. Activated carbon has a large surface area capable of adsorbing the physiologically active substances, gases, alkaloids, toxins and other substances (Mashkovsky, M. D., 1997). This reduces the chemical irritation of the walls of feces. The results of experiments obtained a clear picture of violations of motor-evacuation function of the gastrointestinal tract, continuing for 2 days, which can be considered as a model of constipation, as illustrated by the following examples.

Example 1. 13 outbred white rats-males weighing 190 to 210 g per 1 day prior to the experiment were placed on feeding dry grain blend (oats, wheat, sunflower). Animals had access to automatic drinking bowl with water. After 1 day, animals were placed in individual cages and intragastrically through a probe was administered twice with an interval of 40 min to 1.5 h in 2 ml of 1% starch mucus. Animals had free access to food (grain mixture) and water. After 1 and 2 days was determined by the number of allocated feces. The average weight of feces in the group for the 1st day was 3.4 0.3 g, 2-e d - 3,3 0,1 g (table. 1).

Example 2. 7 outbred white rats-males weighing 190 to 210 g per 1 day prior to the experiment were placed on feeding dry grain blend (oats, wheat, sunflower). Animals had access to automatic drinking bowl with water. After 1 day, animals were placed in individual cages and intragastrically via the probe was injected twice with intervals and free access to food (grain mixture) and water. After 1 and 2 days was determined by the number of allocated feces. The average weight of feces in the group for the 1st day was 3.0 0.2 g, and for the 2nd day - 3,3 0,1 g (table. 1).

Example 3. 14 outbred white rats-males weighing 190 to 210 g per 1 day prior to the experiment were placed on feeding dry grain blend (oats, wheat, sunflower). Animals had access to automatic drinking bowl with water. After 1 day, animals were placed in individual cages and intragastrically through a probe was administered twice with an interval of 40 min to 1.5 h in 2 ml of 10% suspension of activated charcoal in 1% starch mucilage in a total dose of 2 g/kg Animals had free access to food (grain mixture) and water. After 1 and 2 days was determined by the number of allocated feces. The average weight of feces in the group for the 1st day was 2.2 0.2 g, and for the 2nd day - 2,2 0,1 g (table. 1).

Thus, when introduced into the stomach of rats activated carbon in a total dose of 2 g/kg obtained in violation of motor-evacuation function of the gastrointestinal tract, manifested in significantly less faeces within 2 days. A smaller dose of activated charcoal (1 g/kg) does not cause a clear and significant violation of motor-evacuation function of the gastrointestinal tract. Vvedenie introduction into the stomach volume of 1.5 2 ml, and exceeding this amount may cause hyperextension of the stomach content, which in itself can change the motility of the gastrointestinal tract (Zapadnyj I. P. et al., 1974). The increase in the concentration of activated carbon in suspension increases its viscosity, leads to a rapid loss of coal sludge and makes it impossible for introduction into the stomach through a tube.

Sources of information

Pathological physiology /edited by ADO A. D., Ishimbai L. M. - M., 1973, S. 398 - 402.

Mashkovsky M. D. Medicines. - M., 1997, T. 1, S. 336.

Zapadnyj I. P., Zapadnyj Century. And., Zachariah E. A. Laboratory animals. - Kiev, 1974, S. 197 - 205.

The method of modeling constipation, characterized in that the rats injected intragastrically 10% suspension of activated charcoal in a total dose of 2 g/kg

 

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FIELD: medicine, pharmacy.

SUBSTANCE: invention relates to a pharmaceutical composition used for stabilization of homeostasis and arresting pathological processes in the body. Invention proposes a pharmaceutical composition as powder with particles size from 250 to 400 mcm comprising the following components by the first variant, wt.-%: carbon, 10.01-53.02; oxygen, 30.10-53.10; potassium, 0.26-1.99, and calcium, 0.20-31.37, and comprising the following components by the second variant, wt.-%: calcium, 0.35-31.20; carbon, 10.99-50.21; oxygen, 34.55-51.03; sulfur, 0.73-14.81, and phosphorus, 0.08-3.30. Invention provides compensation of trace elements unbalance that causes and accompanies many diseases, possibility for stabilization of trace element homeostasis and arresting pathological processes of different etiology.

EFFECT: improved and valuable medicinal properties of composition.

12 cl, 13 ex

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